Gene Symbol: ACR3
Description: Arr3p
Alias: ACR3, Arr3p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Thorsen M, Lagniel G, Kristiansson E, Junot C, Nerman O, Labarre J, et al. Quantitative transcriptome, proteome, and sulfur metabolite profiling of the Saccharomyces cerevisiae response to arsenite. Physiol Genomics. 2007;30:35-43 pubmed
  2. Wysocki R, Bobrowicz P, Ułaszewski S. The Saccharomyces cerevisiae ACR3 gene encodes a putative membrane protein involved in arsenite transport. J Biol Chem. 1997;272:30061-6 pubmed
    The cluster of three genes, ACR1, ACR2, and ACR3, previously was shown to confer arsenical resistance in Saccharomyces cerevisiae. The overexpression of ACR3 induced high level arsenite resistance...
  3. Ghosh M, Shen J, Rosen B. Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999;96:5001-6 pubmed
    ..Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity...
  4. Bobrowicz P, Wysocki R, Owsianik G, Goffeau A, Ułaszewski S. Isolation of three contiguous genes, ACR1, ACR2 and ACR3, involved in resistance to arsenic compounds in the yeast Saccharomyces cerevisiae. Yeast. 1997;13:819-28 pubmed
    ..Homology searches revealed a cluster of three new open reading frames named ACR1, ACR2 and ACR3. The hypothetical product of the ACR1 gene is similar to the transcriptional regulatory proteins, encoded by YAP1, ..
  5. Thorsen M, Di Y, Tängemo C, Morillas M, Ahmadpour D, Van der Does C, et al. The MAPK Hog1p modulates Fps1p-dependent arsenite uptake and tolerance in yeast. Mol Biol Cell. 2006;17:4400-10 pubmed
    ..Understanding how arsenite/antimonite uptake and toxicity is modulated may prove of value for their use in medical therapy. ..
  6. Di Y, Tamás M. Regulation of the arsenic-responsive transcription factor Yap8p involves the ubiquitin-proteasome pathway. J Cell Sci. 2007;120:256-64 pubmed
    ..arsenic tolerance by stimulating enhanced transcription of the arsenic-specific detoxification genes ACR2 and ACR3. Here, we report that Yap8p is regulated at the level of degradation...
  7. Maciaszczyk Dziubinska E, Wawrzycka D, Sloma E, Migocka M, Wysocki R. The yeast permease Acr3p is a dual arsenite and antimonite plasma membrane transporter. Biochim Biophys Acta. 2010;1798:2170-5 pubmed publisher
    The Acr3p permease from the yeast Saccharomyces cerevisiae is a prototype member of the arsenical resistance-3 (Acr3) family of transporters, which are found in all domains of life...
  8. Maciaszczyk Dziubinska E, Migocka M, Wysocki R. Acr3p is a plasma membrane antiporter that catalyzes As(III)/H(+) and Sb(III)/H(+) exchange in Saccharomyces cerevisiae. Biochim Biophys Acta. 2011;1808:1855-9 pubmed publisher
    ..Saccharomyces cerevisiae as well as in a growing number of prokaryotes and eukaryotes is mediated by members of the Acr3 family of transporters...
  9. Ali W, Isner J, Isayenkov S, Liu W, Zhao F, Maathuis F. Heterologous expression of the yeast arsenite efflux system ACR3 improves Arabidopsis thaliana tolerance to arsenic stress. New Phytol. 2012;194:716-23 pubmed publisher
    ..Here, we heterologously expressed the yeast (Saccharomyces cerevisiae) arsenite efflux transporter ACR3 into Arabidopsis to evaluate how this affects plant tolerance and tissue arsenic contents...

More Information


  1. Maciaszczyk Dziubinska E, Migocka M, Wawrzycka D, Markowska K, Wysocki R. Multiple cysteine residues are necessary for sorting and transport activity of the arsenite permease Acr3p from Saccharomyces cerevisiae. Biochim Biophys Acta. 2014;1838:747-55 pubmed publisher
    ..It has been shown for bacterial Acr3 proteins that just a single cysteine residue, which is located in the middle of the fourth transmembrane region and ..
  2. Markowska K, Maciaszczyk Dziubinska E, Migocka M, Wawrzycka D, Wysocki R. Identification of critical residues for transport activity of Acr3p, the Saccharomyces cerevisiae As(III)/H+ antiporter. Mol Microbiol. 2015;98:162-74 pubmed publisher
    ..a systematic alanine-replacement analysis of charged/polar and aromatic residues that are conserved in the Acr3 family and located in putative transmembrane segments...
  3. Wysocki R, Fortier P, Maciaszczyk E, Thorsen M, Leduc A, Odhagen A, et al. Transcriptional activation of metalloid tolerance genes in Saccharomyces cerevisiae requires the AP-1-like proteins Yap1p and Yap8p. Mol Biol Cell. 2004;15:2049-60 pubmed reside in the nucleus where it mediates enhanced expression of the arsenic detoxification genes ACR2 and ACR3. Using chromatin immunoprecipitation assays, we show that Yap8p is associated with the ACR3 promoter in untreated ..
  4. Maciaszczyk Dziubinska E, Migdal I, Migocka M, Bocer T, Wysocki R. The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel. FEBS Lett. 2010;584:726-32 pubmed publisher
    ..Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux...
  5. Indriolo E, Na G, Ellis D, Salt D, Banks J. A vacuolar arsenite transporter necessary for arsenic tolerance in the arsenic hyperaccumulating fern Pteris vittata is missing in flowering plants. Plant Cell. 2010;22:2045-57 pubmed publisher
    ..Here, we describe the isolation and characterization of two P. vittata genes, ACR3 and ACR3;1, which encode proteins similar to the ACR3 arsenite effluxer of yeast...
  6. Wawrzycka D, Markowska K, Maciaszczyk Dziubinska E, Migocka M, Wysocki R. Transmembrane topology of the arsenite permease Acr3 from Saccharomyces cerevisiae. Biochim Biophys Acta Biomembr. 2017;1859:117-125 pubmed publisher
    b>Acr3 is a plasma membrane transporter, a member of the bile/arsenite/riboflavin transporter (BART) superfamily, which confers high-level resistance to arsenicals in the yeast Saccharomyces cerevisiae...
  7. Bouganim N, David J, Wysocki R, Ramotar D. Yap1 overproduction restores arsenite resistance to the ABC transporter deficient mutant ycf1 by activating ACR3 expression. Biochem Cell Biol. 2001;79:441-8 pubmed
    Ycf1 and Acr3 are transporters that have been previously shown to protect Saccharomyces cerevisiae cells from the toxic effects of arsenite...