Gene Symbol: ABF1
Description: DNA-binding protein ABF1
Alias: BAF1, OBF1, REB2, SBF1, DNA-binding protein ABF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yarragudi A, Miyake T, Li R, Morse R. Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:9152-64 pubmed
    Autonomously replicating sequence binding factor 1 (ABF1) and repressor/activator protein 1 (RAP1) from budding yeast are multifunctional, site-specific DNA-binding proteins, with roles in gene activation and repression, replication, and ..
  2. Kawasaki Y, Kim H, Kojima A, Seki T, Sugino A. Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells. 2006;11:745-56 pubmed
  3. Schlecht U, Erb I, Demougin P, Robine N, Borde V, van Nimwegen E, et al. Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast. Mol Biol Cell. 2008;19:2193-207 pubmed publisher
    The autonomously replicating sequence binding factor 1 (Abf1) was initially identified as an essential DNA replication factor and later shown to be a component of the regulatory network controlling mitotic and meiotic cell cycle ..
  4. Miyake T, Reese J, Loch C, Auble D, Li R. Genome-wide analysis of ARS (autonomously replicating sequence) binding factor 1 (Abf1p)-mediated transcriptional regulation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:34865-72 pubmed
    ..Here we report findings from a genome-wide comparison of the gene expression profiles in the wild-type and abf1-1 temperature-sensitive mutant. The study identifies a total of 86 Abf1p-regulated genes (1...
  5. Miyake T, Loch C, Li R. Identification of a multifunctional domain in autonomously replicating sequence-binding factor 1 required for transcriptional activation, DNA replication, and gene silencing. Mol Cell Biol. 2002;22:505-16 pubmed
    Autonomously replicating sequence-binding factor 1 (ABF1) is a multifunctional, site-specific DNA binding protein that is essential for cell viability in Saccharomyces cerevisiae...
  6. Yarragudi A, Parfrey L, Morse R. Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae. Nucleic Acids Res. 2007;35:193-202 pubmed
    b>Abf1 and Rap1 are general regulatory factors (GRFs) that contribute to transcriptional activation of a large number of genes, as well as to replication, silencing and telomere structure in yeast...
  7. Beinoraviciute Kellner R, Lipps G, Krauss G. In vitro selection of DNA binding sites for ABF1 protein from Saccharomyces cerevisiae. FEBS Lett. 2005;579:4535-40 pubmed
    The autonomously replicating sequence-binding factor 1 (ABF1) from Sacchramoyces cerevisiae is known as a multifunctional DNA binding protein that is involved in transcriptional regulation, DNA-replication, and in restructuring of ..
  8. Lascaris R, Groot E, Hoen P, Mager W, Planta R. Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene. Nucleic Acids Res. 2000;28:1390-6 pubmed
    ..We conclude that Abf1p is a strong organizer of local chromatin structure that appears to act as a nucleosomal boundary factor requiring the downstream T-rich element to create a nucleosome-free region. ..
  9. Yu S, Smirnova J, Friedberg E, Stillman B, Akiyama M, Owen Hughes T, et al. ABF1-binding sites promote efficient global genome nucleotide excision repair. J Biol Chem. 2009;284:966-73 pubmed publisher
    ..We have reported that autonomously replicating sequence-binding factor 1 (ABF1) protein forms a stable complex with Rad7 and Rad16 proteins...

More Information


  1. Aranda C, Colón M, Ishida C, Riego L, DeLuna A, Valenzuela L, et al. Gcn5p contributes to the bidirectional character of the UGA3-GLT1 yeast promoter. Biochem Biophys Res Commun. 2006;348:989-96 pubmed
    ..The phenotype displayed by a double mutant impaired in GCN5 and in the Abf1p binding site indicates that the combined action of these two elements determines the bidirectional capacity of the UGA3-GLT1 intergenic region. ..
  2. Chanet R, Heude M. Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet. 2003;43:337-50 pubmed
    ..genes involved in repair and genome stability (MET18/ MMS19), in the control of origin-firing and/or transcription (ABF1, SRB7), in the S/G2 checkpoint (RAD53), in the Ras-cAMP signal transduction pathway (MKS1), in nuclear pore ..
  3. Bosio M, Fermi B, Spagnoli G, Levati E, Rubbi L, Ferrari R, et al. Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast. Nucleic Acids Res. 2017;45:4493-4506 pubmed publisher
    ..Ribi gene promoters also contain binding sites for one or more General Regulatory Factors (GRFs), most frequently Abf1 and Reb1, and that these factors are enriched in vivo at Ribi promoters...
  4. Upton T, Wiltshire S, Francesconi S, Eisenberg S. ABF1 Ser-720 is a predominant phosphorylation site for casein kinase II of Saccharomyces cerevisiae. J Biol Chem. 1995;270:16153-9 pubmed
    b>ABF1 is a multifunctional phosphoprotein that binds specifically to yeast origins of replication and to transcriptional regulatory sites of a variety of genes...
  5. Feng L, Wang B, Jong A. Saccharomyces cerevisiae Cdc6 stimulates Abf1 DNA binding activity. J Biol Chem. 1998;273:1298-302 pubmed
    In budding yeast Saccharomyces cerevisiae, an ARS binding factor 1 (Abf1) binds to the sequence-specific DNA element involved in DNA replication and transcription...
  6. Zou Y, Yu Q, Bi X. Asymmetric positioning of nucleosomes and directional establishment of transcriptionally silent chromatin by Saccharomyces cerevisiae silencers. Mol Cell Biol. 2006;26:7806-19 pubmed
    ..We conclude that the HML-I and HMR-E silencers promote asymmetric positioning of nucleosomes, leading to unequal potentials of transcriptional silencing on their sides and, hence, directional silencing. ..
  7. Ganapathi M, Palumbo M, Ansari S, He Q, Tsui K, Nislow C, et al. Extensive role of the general regulatory factors, Abf1 and Rap1, in determining genome-wide chromatin structure in budding yeast. Nucleic Acids Res. 2011;39:2032-44 pubmed publisher
    ..Here, we use high resolution tiling arrays to examine the contributions of two general regulatory factors, Abf1 and Rap1, to nucleosome occupancy in Saccharomyces cerevisiae...
  8. Diffley J, Stillman B. Purification of a yeast protein that binds to origins of DNA replication and a transcriptional silencer. Proc Natl Acad Sci U S A. 1988;85:2120-4 pubmed
    ..ABF-I also binds within an ARS at the HMR silent mating locus. This ARS functions as a cis-acting transcriptional silencer, and the ABF-I-binding site within this ARS is important for both ARS and silencer function. ..
  9. Fermi B, Bosio M, Dieci G. Promoter architecture and transcriptional regulation of Abf1-dependent ribosomal protein genes in Saccharomyces cerevisiae. Nucleic Acids Res. 2016;44:6113-26 pubmed publisher
    In Saccharomyces cerevisiae, ribosomal protein gene (RPG) promoters display binding sites for either Rap1 or Abf1 transcription factors...
  10. Shin Y, Goo D, So I, Rhode P, Campbell J, Kim J. Isolation and characterization of Saccharomyces cerevisiae SAB2, a suppressor gene for temperature-sensitive phenotype of ARS-binding factor 1 mutant. Biochem Mol Biol Int. 1996;40:915-21 pubmed
    A high-copy number suppressor of yeast abf1-5 mutant, a temperature-sensitive lethal mutant, was isolated and named SAB2 (suppressor of ABF1)...
  11. Zhou W, Ryan J, Zhou H. Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem. 2004;279:32262-8 pubmed
    ..Taken together, these results show that protein sumoylation is broadly involved in many cellular functions and this mass spectrometry-based proteomic approach is useful in studying the regulation of protein sumoylation in the cells. ..
  12. Matveenko A, Belousov M, Bondarev S, Moskalenko S, Zhouravleva G. [Identification of new genes that affect [PSI^(+)] prion toxicity in Saccharomyces cerevisiae yeast]. Mol Biol (Mosk). 2016;50:803-813 pubmed
    ..It was found that ABF1, FKH2, and REB1 overexpression decreased the growth of strains in a prion-dependent manner and, thus, might ..
  13. Loo S, Laurenson P, Foss M, Dillin A, Rine J. Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae. Genetics. 1995;141:889-902 pubmed
    ..This screen identified temperature-sensitive alleles of ORC2 and ORC5, as described elsewhere, and ABF1, NPL3, and YCL54, as described here...
  14. Yoo H, Jung S, Kim Y, Kim J, Jung G, Rho H. Transcriptional control of the Saccharomyces cerevisiae ADH1 gene by autonomously replicating sequence binding factor 1. Curr Microbiol. 1995;31:163-8 pubmed
    Autonomously replicating sequence (ARS)-binding factor 1 (ABF1) is a multifunctional protein involved in transcriptional activation and repression, as well as DNA replication, in yeast...
  15. So I, Rhode P, Campbell J, Kim J. Molecular cloning and characterization of the Saccharomyces cerevisiae SAB1 gene that suppresses a temperature-sensitive phenotype of the ARS-binding factor 1 mutant. Mol Cells. 1997;7:532-6 pubmed
    A high-copy number suppressor gene of the yeast temperature-sensitive lethal abf1 mutant was isolated and named SAB1 (suppressor of ABF1)...
  16. Woo D, Phang T, Trawick J, Poyton R. Multiple pathways of mitochondrial-nuclear communication in yeast: intergenomic signaling involves ABF1 and affects a different set of genes than retrograde regulation. Biochim Biophys Acta. 2009;1789:135-45 pubmed publisher
    ..Together, these findings indicate that intergenomic signaling is distinct from retrograde regulation both in the nuclear genes that it regulates and in the way in which it affects their expression. ..
  17. Cremona C, Sarangi P, Yang Y, Hang L, Rahman S, Zhao X. Extensive DNA damage-induced sumoylation contributes to replication and repair and acts in addition to the mec1 checkpoint. Mol Cell. 2012;45:422-32 pubmed publisher
    ..We also show that DNA damage-induced sumoylation does not require Mec1 checkpoint signaling, and the presence of both enables optimal DNA damage resistance. ..
  18. McBroom L, Sadowski P. DNA bending by Saccharomyces cerevisiae ABF1 and its proteolytic fragments. J Biol Chem. 1994;269:16461-8 pubmed
    The ABF1 protein of the yeast Saccharomyces cerevisiae has been found to bend the DNA containing the target site for DNA binding...
  19. Yu S, Owen Hughes T, Friedberg E, Waters R, Reed S. The yeast Rad7/Rad16/Abf1 complex generates superhelical torsion in DNA that is required for nucleotide excision repair. DNA Repair (Amst). 2004;3:277-87 pubmed
    ..We previously isolated a stable heterotrimeric complex of Rad7/Rad16/Abf1 from yeast which functions in the conserved global genome repair (GGR) pathway...
  20. Buchman A, Kimmerly W, Rine J, Kornberg R. Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:210-25 pubmed
    ..Together, these results indicate that both GRFI and ABFI play multiple roles within the cell. ..
  21. Loch C, Mosammaparast N, Miyake T, Pemberton L, Li R. Functional and physical interactions between autonomously replicating sequence-binding factor 1 and the nuclear transport machinery. Traffic. 2004;5:925-35 pubmed
    ..We propose that the link between Abf1p and the nuclear transport machinery may also be important for partitioning multiple Abf1p-mediated nuclear processes. ..
  22. Silver H, Nissley J, Reed S, Hou Y, Johnson E. A role for SUMO in nucleotide excision repair. DNA Repair (Amst). 2011;10:1243-51 pubmed publisher
    ..Collectively, these results suggest that SIZ-dependent sumoylation may modulate the activity of multiple proteins to promote efficient NER. ..
  23. Reed S, Akiyama M, Stillman B, Friedberg E. Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair. Genes Dev. 1999;13:3052-8 pubmed
    ..Here, we report the identification of autonomously replicating sequence binding factor 1 (ABF1) as a component of the Rad7/Rad16 NER subcomplex...
  24. Denison C, Rudner A, Gerber S, Bakalarski C, Moazed D, Gygi S. A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics. 2005;4:246-54 pubmed
    ..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes. ..
  25. Bodmer Glavas M, Edler K, Barberis A. RNA polymerase II and III transcription factors can stimulate DNA replication by modifying origin chromatin structures. Nucleic Acids Res. 2001;29:4570-80 pubmed
    ..The natural ARS1 activator Abf1 and the other transcription factors that stimulated replication remodeled chromatin in a very similar manner...