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Genomes and Genes | rpsLSummaryGene Symbol: rpsL Description: 30S ribosomal subunit protein S12 Alias: ECK3329, JW3304, asuB, strA Species: Top Publications
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Publications
The contribution of a novel ribosomal S12 mutation to aminoglycoside resistance of Escherichia coli mutantsA E Gill
Division of Medical Microbiology, School of Biomedical and Clinical Laboratory Sciences, University of Edinburgh, Medical School, Teviot Place, Edinburgh EH8 9AG, UK
J Chemother 16:347-9. 2004..DNA sequencing of a 423bp region of the rpsL gene encoding S12 revealed a novel Lys87-->Glu mutation in the streptomycin selected resistant mutants, while there ..- Ribosomal protein S12 and aminoglycoside antibiotics modulate A-site mRNA cleavage and transfer-messenger RNA activity in Escherichia coliLaura E Holberger
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106 9610, USA
J Biol Chem 284:32188-200. 2009..Streptomycin did not inhibit A-site cleavage in rpsL mutants, which express streptomycin-resistant variants of ribosomal protein S12... - The 30S ribosomal P site: a function of 16S rRNAHarry F Noller
Department of Molecular, Cell and Developmental Biology, Center for Molecular Biology of RNA, University of California at Santa Cruz, CA 95064, USA
FEBS Lett 579:855-8. 2005..Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells... - The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunitsJ Mattias Lövgren
Department of Molecular Biology, Umea University, SE 90187 Umea, Sweden
RNA 10:1798-812. 2004..A GST-RimM but not a GST-RimM(YY-->AA) protein bound strongly to S19 in 30S. Thus, RimM likely facilitates maturation of the region of the head of 30S that contains S13 and S19 as well as helices 31 and 33b... - A novel GTPase activated by the small subunit of ribosomeHyouta Himeno
Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036 8561, Japan
Nucleic Acids Res 32:5303-9. 2004..We also found that 17S RNA, a putative precursor of 16S rRNA, was contained in the small subunit of the ribosome from the RsgA-deletion strain. RsgA is a novel GTPase that might provide a new insight into the function of ribosome... - Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitroDenis M Daigle
Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
J Bacteriol 186:1381-7. 2004..Taken together, these data indicate that the YjeQ protein participates in a guanine nucleotide-dependent interaction with the ribosome and implicate this conserved, essential GTPase as a novel factor in ribosome function... - The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coliT Hosaka
National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
Mol Genet Genomics 271:317-24. 2004Resistance to streptomycin in bacterial cells often results from a mutation in the rpsL gene that encodes the ribosomal protein S12... - The DnaK chaperone system facilitates 30S ribosomal subunit assemblyJennifer A Maki
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames 50011, USA
Mol Cell 10:129-38. 2002..These studies reveal a novel role for the DnaK/hsp70 chaperone system, in addition to its well-documented role in protein folding, and suggest that 30S subunit assembly can be facilitated... - Strand asymmetry of +1 frameshift mutagenesis at a homopolymeric run by DNA polymerase III holoenzyme of Escherichia coliM Seki
Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
J Biol Chem 274:33313-9. 1999We have recently shown that single-base frameshifts were predominant among mutations induced within the rpsL target sequence upon oriC plasmid DNA replication in vitro... - Assaying RNA chaperone activity in vivo using a novel RNA folding trapE Clodi
Institute of Microbiology and Genetics, Vienna Biocenter, Dr Bohrgasse 9, A 1030 Vienna, Austria
EMBO J 18:3776-82. 1999..Thus, this structural trap enables detection of RNA chaperone activity in vivo... - Modelling in Escherichia coli of mutations in mitoribosomal protein S12: novel mutant phenotypes of rpsLJ M Toivonen
Institute of Medical Technology, Tampere, Finland
Mol Microbiol 31:1735-46. 1999The rpsL gene of Escherichia coli encodes the highly conserved rps12 protein of the ribosomal accuracy centre. We have used the E... - A ribosomal function is necessary for efficient splicing of the T4 phage thymidylate synthase intron in vivoK Semrad
Institute of Microbiology and Genetics, Vienna Biocenter, 1030 Wien, Austria
Genes Dev 12:1327-37. 1998..Point mutations in this region efficiently alleviate the effect of a nonsense codon. We infer from these results that the ribosome acts as an RNA chaperone to facilitate proper folding of the intron... - Host mutations (miaA and rpsL) reduce tetracycline resistance mediated by Tet(O) and Tet(M)D E Taylor
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada
Antimicrob Agents Chemother 42:59-64. 1998..In addition, mutations in E. coli rpsL genes, generating both streptomycin-resistant and streptomycin-dependent strains, were also shown to reduce the .. - Induction of the heat shock regulon in response to increased mistranslation requires oxidative modification of the malformed proteinsAsa Fredriksson
Department of Cell and Molecular Biology Microbiology, Goteborg University, Medicinaregatan 9C, 413 90 Goteborg, Sweden
Mol Microbiol 59:350-9. 2006..The data further suggest that it is the oxidative modification of mistranslated DnaK substrates rather than oxidation of DnaK itself that triggers Hsp gene expression upon starvation... - Mistranslation and genetic variability: the effect of streptomycinRosa Nagel
CEFYBO, CONICET, Serrano 669, Buenos Aires, Argentina
Mutat Res 601:162-70. 2006..coli map that relieves the high translational fidelity conferred by the rpsL mutation... - An array of Escherichia coli clones over-expressing essential proteins: a new strategy of identifying cellular targets of potent antibacterial compoundsH Howard Xu
Department of Biological Sciences, California State University, Los Angeles, 90032, USA
Biochem Biophys Res Commun 349:1250-7. 2006..Target identification via target protein over-expression was demonstrated using both mixed clone and individual clone assay formats... - A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coliDevashish Rath
Molecular Biology Division, Bhabha Atomic Research Center, Trombay, Mumbai 400 085, India
Res Microbiol 160:576-80. 2009..We provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells... - Concurrent nucleation of 16S folding and induced fit in 30S ribosome assemblyTadepalli Adilakshmi
T C Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218 2685, USA
Nature 455:1268-72. 2008..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA... - Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coliJunmei Zhang
Department of Biochemistry, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Mol Cell Proteomics 8:215-25. 2009..Furthermore, we demonstrate that bacterial lysine acetylation is regulated in response to stress stimuli... - Selection of random RNA fragments as method for searching for a site of regulation of translation of E. coli streptomycin mRNA by ribosomal protein S7A V Surdina
Belozersky Institute of Physico Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia
Biochemistry (Mosc) 73:652-9. 2008..Location of S7 binding site on the mRNA, as well as putative mode of regulation of coupled translation of S12 and S7 cistrons have been hypothesized... - Synthesis of beta-(S-methyl)thioaspartic acid and derivativesJorge Heredia-Moya
Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, DHHS, Bethesda, MD 20892, USA
Bioorg Med Chem 16:5908-13. 2008..Following this key transformation, we were able to prepare protected beta-(S-methyl)thioaspartic acid derivative suitable for peptide coupling... - Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) RecombinationRalf Heermann
Ludwig Maximilians Universitat Munchen, Department Biologie I, Bereich Mikrobiologie, Maria Ward Str, 1a, D 80638 München, Germany
Microb Cell Fact 7:14. 2008..abstract:.. - New light on methylthiolation reactionsM Fontecave
Laboratoire de Chimie et Biologie des Metaux, UMR 5249, Universite Joseph Fourier, CEA, CNRS, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
Chem Biol 15:209-10. 2008..2008) catalyzes the methylthiolation of aspartate 88 of the S12 ribosomal protein in Escherichia coli and shows a strong similarity with the iron-sulfur enzyme MiaB involved in the methylthiolation of tRNAs... - RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coliBrian P Anton
New England Biolabs, Ipswich, MA 01938, USA
Proc Natl Acad Sci U S A 105:1826-31. 2008..The initial results presented here constitute a bioinformatics-driven prediction with preliminary experimental validation that should serve as the starting point for several interesting lines of further inquiry... - Structural Insights into ribosome recycling factor interactions with the 70S ribosomeRaj D Pai
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
J Mol Biol 376:1334-47. 2008..The structures also reveal contacts between domain II of RRF and protein S12 in the 30S subunit that may also play a role in ribosome recycling... - Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosomeDivya Sharma
Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
J Mol Biol 374:1065-76. 2007.... - Modulation of 16S rRNA function by ribosomal protein S12Anton Vila-Sanjurjo
Berkeley Center for Synthetic Biology, University of California, Berkeley, 717 Potter St, Berkeley, CA 94720 3224, USA
Biochim Biophys Acta 1769:462-71. 2007..The C912U mutation on its own confers resistance to streptomycin and restricts miscoding, properties that distinguish it from a majority of the previously described error-promoting ram mutants that also reverse streptomycin dependence... - Roles of RecA protein in spontaneous mutagenesis in Escherichia coliSatoshi Kanie
Department of Molecular Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan
Genes Genet Syst 82:99-108. 2007..both forward mutations and recombination events occurring within about 600 base pairs of a transgenic rpsL target sequence located on Escherichia coli chromosome... - IS1 transposition is enhanced by translation errors and by bacterial growth at extreme glucose levelsArun S Kharat
Plasticité et Expression des Génomes Microbiens, CNRS FRE2383 CEA LRC12 Université Joseph Fourier, Grenoble, France
Acta Biochim Pol 53:729-38. 2006..Since starved cells are known to enhance ribosome frameshifting, our data suggests that growth conditions applied in this study could affect IS1 transposition by increasing translation infidelity... - Ribosomal proteins S12 and S13 function as control elements for translocation of the mRNA:tRNA complexAnthony R Cukras
Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mol Cell 12:321-8. 2003..These data support a model where S12 and S13 function as control elements for the more ancient rRNA- and tRNA-driven movements of translocation... - Beta-methylthio-aspartic acid: identification of a novel posttranslational modification in ribosomal protein S12 from Escherichia coliJ A Kowalak
Department of Biochemistry, University of Washington, Seattle 98195, USA
Protein Sci 5:1625-32. 1996..The modified residue is located at position 88 in ribosomal protein S12 from Escherichia coli, a phylogenetically conserved protein that has been implicated in maintaining translational accuracy of the ribosome... - Dissociation rates of peptidyl-tRNA from the P-site of E.coli ribosomesR Karimi
Department of Molecular Biology, BMC, Box 590, S 751 24, Sweden
EMBO J 15:1149-54. 1996..The results are also used to interpret some in vivo experiments on translational processivity... - Mutations in ribosomal proteins S4 and S12 influence the higher order structure of 16 S ribosomal RNAP N Allen
Thimann Laboratories, University of California, Santa Cruz 95064
J Mol Biol 208:457-68. 1989..This suggests that the opposing effects of these two classes of mutations influence the proof-reading process by somewhat different mechanisms... - The nucleotide sequence of rpsL and its flanking regions in Salmonella typhimuriumD Hughes
Department of Molecular Biology, Uppsala University, Sweden
Gene 104:123-4. 1991The ribosomal protein (r-protein)-encoding gene, rpsL, and regions flanking it, from Salmonella typhimurium, have been sequenced directly from polymerase chain reaction-amplified chromosomal DNA... - Base 2661 in Escherichia coli 23S rRNA influences the binding of elongation factor Tu during protein synthesis in vivoS Tapio
Department of Microbiology, Stockholm University, Sweden
Eur J Biochem 202:981-4. 1991..either the wild-type or the mutant gene (tufA) for EF-Tu as well as normal or mutant ribosomal protein S12 (rpsL)... - Interaction between 16S ribosomal RNA and ribosomal protein S12: differential effects of paromomycin and streptomycinM O'CONNOR
Section of Biochemistry, Brown University, Providence, RI 02912
Biochimie 73:1493-500. 1991..The results demonstrate a functional interaction between the 1409-1491 region of rRNA and ribosomal protein S12... - A functional pseudoknot in 16S ribosomal RNAT Powers
Sinsheimer Laboratories, University of California, Santa Cruz 95064
EMBO J 10:2203-14. 1991..Data from earlier in vitro assembly studies suggest that the pseudoknot structure is stabilized by ribosomal protein S12, mutations in which have long been known to confer streptomycin resistance and dependence... - Kinetic properties of Escherichia coli ribosomes with altered forms of S12N Bilgin
University of Uppsala, Department of Molecular Biology, Sweden
J Mol Biol 224:1011-27. 1992..We use our in vitro findings to discuss the in vivo physiology of these mutants as well as mechanistic features of E. coli translation... - Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesisA R Timms
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Great Britain
Mol Gen Genet 232:89-96. 1992..These mutants, characterized as streptomycin resistant (Smr) or dependent (Smd), carry mutations in the rpsL gene. This gene was amplified using the polymerase chain reaction and sequenced... - Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coliU Johanson
Department of Molecular Biology, Uppsala University, Sweden
Gene 120:93-8. 1992..The order of conservation at the nt and aa level is rpsL greater than tufA greater than rpsG greater than f usA... - Primary stucture of protein S12 from the small Escherichia coli ribosomal subunitG Funatsu
FEBS Lett 73:12-7. 1977 - Selective perturbation of G530 of 16 S rRNA by translational miscoding agents and a streptomycin-dependence mutation in protein S12T Powers
Sinsheimer Laboratories, University of California, Santa Cruz 95064
J Mol Biol 235:156-72. 1994..These results are discussed in terms of a model wherein the conformation of the 530 loop is correlated with the affinity of the ribosome for elongation factor Tu... - Escherichia coli proteins, including ribosomal protein S12, facilitate in vitro splicing of phage T4 introns by acting as RNA chaperonesT Coetzee
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, Albany 12201 0509
Genes Dev 8:1575-88. 1994..These results suggest that this protein facilitates splicing by acting as an RNA chaperone, promoting the assembly of the catalytically active tertiary structure of ribozymes... - Peptide environment of the peptidyl transferase center from Escherichia coli 70 S ribosomes as determined by thermoaffinity labeling with dihydrospiramycinO Bischof
Max Delbruck Centrum fur Molekulare Medizin, Abteilung Proteinchemie, Berlin Buch, Germany
J Biol Chem 270:23060-4. 1995..This approach is a valuable tool to characterize the binding site of spiramycin as well as the peptidyl transferase center at the molecular level... - Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translationK Saito
Department of Biological Chemistry, University of California, Irvine 92717 1700
J Mol Biol 235:111-24. 1994The str operon of Escherichia coli consists of the genes for ribosomal proteins S12 (rpsL) and S7 (rpsG) and elongation factors G (fusA) and Tu (tufA)... - IF-3 crosslinking to Escherichia coli ribosomal 30 S subunits by three different light-dependent procedures: identification of 30 S proteins crosslinked to IF-3--utilization of a new two-stage crosslinking reagent, p-nitrobenzylmaleimideB S Cooperman
Arch Biochem Biophys 208:554-62. 1981 - DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coliL E Post
Cell 15:215-29. 1978..Extensive sequence similarity between the str and spc promoter regions is found downstream from the Pribnow box-that is, in a transcribed region preceding the translation start sites... - Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunitsL A MacKeen
J Biol Chem 255:10526-31. 1980..The target proteins have been identified as S7, S11, S12, S18, and S21 by immunochemical techniques... - DNA sequences from the str operon of Escherichia coliL E Post
J Biol Chem 255:4660-6. 1980..operon at 72 min on the Escherichia coli chromosome contains genes for ribosomal proteins (r-proteins) S12 (str or rpsL) and S7 (rpsG) and elongation factors G (fus) and Tu (tufA)... - Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolaneG Boileau
Biochemistry 22:3162-70. 1983..Cross-links between factors IF1-IF2 and IF3-IF2 were also identified. A model integrating these findings with others on the protein topography of the ribosome is presented... - Codon-specific missense errors in vivoF Bouadloun
Department of Molecular Biology, University of Uppsala, Sweden
EMBO J 2:1351-6. 1983..to 10(-3) for wild-type bacteria, decreases to 4 x 10(-4) in streptomycin-resistant bacteria containing mutant S12 (rpsL), and is virtually unchanged in Ram bacteria containing mutant S4 (rpsD)... - Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNAS Stern
Thimann Laboratories, University of California, Santa Cruz 95064
J Mol Biol 201:683-95. 1988..Altogether these results show that many of the small subunit proteins, which have previously been shown to be functionally important, appear to be associated with functionally implicated segments of 16 S rRNA... - A conformational switch in Escherichia coli 16S ribosomal RNA during decoding of messenger RNAJ S Lodmell
Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
Science 277:1262-7. 1997..These data indicate that the switch affects codon-anticodon arrangement and proper selection of tRNA at the ribosomal A site, and that the switch is a fundamental mechanism in all ribosomes...