appA

Summary

Gene Symbol: appA
Description: AppA, antirepressor of ppsR, sensor of blue light
Species: Rhodobacter sphaeroides 2.4.1

Top Publications

  1. Han Y, Meyer M, Keusgen M, Klug G. A haem cofactor is required for redox and light signalling by the AppA protein of Rhodobacter sphaeroides. Mol Microbiol. 2007;64:1090-104 pubmed
    The AppA protein of Rhodobacter sphaeroides is unique in its ability to sense and transmit redox signals as well as light signals...
  2. Anderson S, Dragnea V, Masuda S, Ybe J, Moffat K, Bauer C. Structure of a novel photoreceptor, the BLUF domain of AppA from Rhodobacter sphaeroides. Biochemistry. 2005;44:7998-8005 pubmed publisher
    The flavin-binding BLUF domain of AppA represents a new class of blue light photoreceptors that are present in a number of bacterial and algal species. The dark state X-ray structure of this domain was determined at 2.3 A resolution...
  3. Dragnea V, Waegele M, Balascuta S, Bauer C, Dragnea B. Time-resolved spectroscopic studies of the AppA blue-light receptor BLUF domain from Rhodobacter sphaeroides. Biochemistry. 2005;44:15978-85 pubmed publisher
    b>AppA is a blue-light and redox-responding regulator of photosynthesis gene expression in Rhodobacter sphaeroides...
  4. Udvarhelyi A, Domratcheva T. Glutamine rotamers in BLUF photoreceptors: a mechanistic reappraisal. J Phys Chem B. 2013;117:2888-97 pubmed publisher
    ..Finally, we demonstrate that the tautomerized glutamine is consistent with our criteria and observations of the BLUF light state...
  5. Laptenok S, Lukacs A, Brust R, Haigney A, Gil A, Towrie M, et al. Electron transfer quenching in light adapted and mutant forms of the AppA BLUF domain. Faraday Discuss. 2015;177:293-311 pubmed publisher
    ..resolved infra-red (TRIR) spectroscopy to investigate the primary photophysics of the BLUF domain of the protein AppA (AppABLUF) a light activated antirepressor. Here a number of mutations at Y21 and W104 in AppABLUF are investigated...
  6. Happ H, Braatsch S, Broschek V, Osterloh L, Klug G. Light-dependent regulation of photosynthesis genes in Rhodobacter sphaeroides 2.4.1 is coordinately controlled by photosynthetic electron transport via the PrrBA two-component system and the photoreceptor AppA. Mol Microbiol. 2005;58:903-14 pubmed publisher
    ..this signal leads to activation of photosynthesis genes and interferes with a blue-light repression mediated by the AppA photoreceptor and the PpsR transcriptional repressor in R. sphaeroides...
  7. Gomelsky L, Moskvin O, Stenzel R, Jones D, Donohue T, Gomelsky M. Hierarchical regulation of photosynthesis gene expression by the oxygen-responsive PrrBA and AppA-PpsR systems of Rhodobacter sphaeroides. J Bacteriol. 2008;190:8106-14 pubmed publisher
    ..We also more accurately assessed the spectrum of gene targets of the PrrBA system. First, expression of the appA gene, encoding the PpsR antirepressor, is PrrA dependent, which establishes one level of hierarchical dominance of ..
  8. Metz S, J ger A, Klug G. In vivo sensitivity of blue-light-dependent signaling mediated by AppA/PpsR or PrrB/PrrA in Rhodobacter sphaeroides. J Bacteriol. 2009;191:4473-7 pubmed publisher
    ..of photosynthesis complexes in Rhodobacter sphaeroides is regulated in a redox- and light-dependent manner by the AppA/PpsR and PrrB/PrrA systems...
  9. Arents J, Perez M, Hendriks J, Hellingwerf K. On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein. FEBS Lett. 2011;585:167-72 pubmed publisher
    The redox-midpoint potential of the FAD chromophore in the BLUF domain of anti-transcriptional regulator AppA from Rhodobacter sphaeroides equals ?-260mV relative to the calomel electrode...

More Information

Publications14

  1. Goyal P, Hammes Schiffer S. Role of active site conformational changes in photocycle activation of the AppA BLUF photoreceptor. Proc Natl Acad Sci U S A. 2017;114:1480-1485 pubmed publisher
    ..Free-energy simulations elucidate the active site conformations in the AppA (activation of photopigment and puc expression) BLUF domain before and following photoexcitation...
  2. Gomelsky M, Kaplan S. appA, a novel gene encoding a trans-acting factor involved in the regulation of photosynthesis gene expression in Rhodobacter sphaeroides 2.4.1. J Bacteriol. 1995;177:4609-18 pubmed
    ..4.1, has been identified. The isolation of this gene, designated appA (activation of photopigment and puc expression), was based on its ability, when provided in extra copies, to ..
  3. Jung A, Reinstein J, Domratcheva T, Shoeman R, Schlichting I. Crystal structures of the AppA BLUF domain photoreceptor provide insights into blue light-mediated signal transduction. J Mol Biol. 2006;362:717-32 pubmed publisher
    ..of the dark-adapted state and of a photo-excited, red-shifted photocycle intermediate of the BLUF unit of AppA, a purple bacterial photoreceptor involved in the light-dependent regulation of photosynthesis gene expression...
  4. Yin L, Dragnea V, Feldman G, Hammad L, Karty J, Dann C, et al. Redox and light control the heme-sensing activity of AppA. MBio. 2013;4:e00563-13 pubmed publisher
    The DNA binding activity of the photosystem-specific repressor PpsR is known to be repressed by the antirepressor AppA. AppA contains a blue-light-absorbing BLUF domain and a heme-binding SCHIC domain that controls the interaction of AppA ..
  5. J ger A, Braatsch S, Haberzettl K, Metz S, Osterloh L, Han Y, et al. The AppA and PpsR proteins from Rhodobacter sphaeroides can establish a redox-dependent signal chain but fail to transmit blue-light signals in other bacteria. J Bacteriol. 2007;189:2274-82 pubmed publisher
    The AppA protein of Rhodobacter sphaeroides has the unique ability to sense and transmit redox and light signals...