Vps4a

Summary

Gene Symbol: Vps4a
Description: vacuolar protein sorting 4 homolog A
Alias: vacuolar protein sorting-associated protein 4A, vacuolar protein sorting 4a, vacuolar protein sorting protein 4a
Species: rat
Products:     Vps4a

Top Publications

  1. Bajorek M, Morita E, Skalicky J, Morham S, Babst M, Sundquist W. Biochemical analyses of human IST1 and its function in cytokinesis. Mol Biol Cell. 2009;20:1360-73 pubmed publisher
    ..In contrast, IST1 depletion did not inhibit human immunodeficiency virus-1 budding. Thus, IST1 and CHMP1 act together to recruit and modulate specific VPS4 activities required during the final stages of cell division. ..
  2. Kieffer C, Skalicky J, Morita E, De Domenico I, Ward D, Kaplan J, et al. Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding. Dev Cell. 2008;15:62-73 pubmed publisher
  3. Garrus J, von Schwedler U, Pornillos O, Morham S, Zavitz K, Wang H, et al. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001;107:55-65 pubmed
    ..These observations suggest that retroviruses bud by appropriating cellular machinery normally used in the Vps pathway to form multivesicular bodies. ..
  4. Stuchell Brereton M, Skalicky J, Kieffer C, Karren M, Ghaffarian S, Sundquist W. ESCRT-III recognition by VPS4 ATPases. Nature. 2007;449:740-4 pubmed
    ..Here we show that the microtubule interacting and transport (MIT) domains of human VPS4A and VPS4B bind conserved sequence motifs located at the carboxy termini of the CHMP1-3 class of ESCRT-III proteins...
  5. Morita E, Sandrin V, Chung H, Morham S, Gygi S, Rodesch C, et al. Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. EMBO J. 2007;26:4215-27 pubmed
    ..These experiments suggest that the ESCRT pathway may be recruited to facilitate analogous membrane fission events during HIV budding, MVB vesicle formation, and the abscission stage of cytokinesis. ..
  6. Scott A, Chung H, Gonciarz Swiatek M, Hill G, Whitby F, Gaspar J, et al. Structural and mechanistic studies of VPS4 proteins. EMBO J. 2005;24:3658-69 pubmed
  7. Langelier C, von Schwedler U, Fisher R, De Domenico I, White P, Hill C, et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006;80:9465-80 pubmed
    ..These observations indicate that there are probably multiple pathways for protein sorting/MVB vesicle formation in human cells and that HIV-1 does not utilize an ESCRT-II-dependent pathway to leave the cell. ..
  8. Bishop N, Woodman P. ATPase-defective mammalian VPS4 localizes to aberrant endosomes and impairs cholesterol trafficking. Mol Biol Cell. 2000;11:227-39 pubmed
    ..The phenotype described here is largely consistent with the defects in vacuolar sorting associated with class E vps mutants in yeast, and a role for mammalian VPS4 is discussed in this context. ..
  9. Dores M, Chen B, Lin H, Soh U, Paing M, Montagne W, et al. ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting. J Cell Biol. 2012;197:407-19 pubmed publisher
    ..This study reveals a novel MVB/lysosomal sorting pathway for signaling receptors that bypasses the requirement for ubiquitination and ubiquitin-binding ESCRTs and may be applicable to a subset of GPCRs containing YPX(n)L motifs. ..

More Information

Publications22

  1. Cashikar A, Shim S, Roth R, Maldazys M, Heuser J, Hanson P. Structure of cellular ESCRT-III spirals and their relationship to HIV budding. elife. 2014;3: pubmed publisher
    ..Interpolating between the observed structures suggests a new role for Vps4 in separating ESCRT-III from Gag or other cargo to allow centripetal growth of a neck constricting ESCRT-III spiral...
  2. Tanaka H, Fujita H, Katoh H, Mori K, Negishi M. Vps4-A (vacuolar protein sorting 4-A) is a binding partner for a novel Rho family GTPase, Rnd2. Biochem J. 2002;365:349-53 pubmed
    ..When Rnd2 was co-expressed with Vps4-A(E228Q), Rnd2 was recruited to the Vps4-A-bound early endosomes. These results suggest that Rnd2 is involved in the regulation of endosomal trafficking via direct binding to Vps4-A. ..
  3. Sahu R, Kaushik S, Clement C, Cannizzo E, Scharf B, Follenzi A, et al. Microautophagy of cytosolic proteins by late endosomes. Dev Cell. 2011;20:131-9 pubmed publisher
    ..Therefore, we propose that endosomal microautophagy shares molecular components with both the endocytic and autophagic pathways. ..
  4. Thoresen S, Campsteijn C, Vietri M, Schink K, Liestøl K, Andersen J, et al. ANCHR mediates Aurora-B-dependent abscission checkpoint control through retention of VPS4. Nat Cell Biol. 2014;16:550-60 pubmed publisher
    ..We propose that the abscission checkpoint is mediated by ANCHR and CHMP4C through retention of VPS4 at the midbody ring. ..
  5. Morita E, Sandrin V, Alam S, Eckert D, Gygi S, Sundquist W. Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell Host Microbe. 2007;2:41-53 pubmed
    ..Taken together, these studies define the composition of human ESCRT-I complexes and indicate that the MVB12 subunits play a unique role in regulating ESCRT-mediated virus budding. ..
  6. Scheuring S, Röhricht R, Schöning Burkhardt B, Beyer A, Muller S, Abts H, et al. Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking. J Mol Biol. 2001;312:469-80 pubmed
    ..Based on these results, we conclude that both human VPS4 proteins are involved in intracellular protein trafficking, presumably at a late endosomal protein transport step, similar to the Vps4p in yeast. ..
  7. Howard T, Stauffer D, Degnin C, Hollenberg S. CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins. J Cell Sci. 2001;114:2395-404 pubmed
    ..These observations identify the conserved CHMP/Chmp family as a set of proteins fundamental to understanding multivesicular body sorting in eukaryotic organisms. ..
  8. Sandrin V, Sundquist W. ESCRT requirements for EIAV budding. Retrovirology. 2013;10:104 pubmed publisher
    ..and mutant constructs revealed budding requirements for the following ESCRT proteins: ALIX, CHMP4B, CHMP2A and VPS4A or VPS4B...
  9. Ren J, Yuan D, Xie L, Tao X, Duan C, Bao Y, et al. Up-regulation of Vps4A promotes neuronal apoptosis after intracerebral hemorrhage in adult rats. Metab Brain Dis. 2017;32:565-575 pubmed publisher
    ..sorting 4, belongs to ATPases Associated with diverse cellular Activities (AAA) protein family which is made up of Vps4A and Vps4B...
  10. Rusten T, Stenmark H. How do ESCRT proteins control autophagy?. J Cell Sci. 2009;122:2179-83 pubmed publisher
    ..We propose that the involvement of ESCRT proteins in the fusion of autophagosomes with the endolysosomal system is the most plausible model. ..
  11. Silvestri L, Ruthel G, Kallstrom G, Warfield K, Swenson D, Nelle T, et al. Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction. J Infect Dis. 2007;196 Suppl 2:S264-70 pubmed
    ..These data show that EBOV can use vps proteins independently of TSG101 for budding and reveal VPS4 as a potential target for filovirus therapeutics. ..
  12. Sachse M, Strous G, Klumperman J. ATPase-deficient hVPS4 impairs formation of internal endosomal vesicles and stabilizes bilayered clathrin coats on endosomal vacuoles. J Cell Sci. 2004;117:1699-708 pubmed
    ..Moreover, our data suggest that disassembly of the coat is required for the formation of internal vesicles. ..
  13. Beyer A, Scheuring S, Müller S, Mincheva A, Lichter P, Köhrer K. Comparative sequence and expression analyses of four mammalian VPS4 genes. Gene. 2003;305:47-59 pubmed
    ..databases from completely or partially sequenced organisms revealed the existence of up to three paralogues, VPS4a, -b, and -c...