Gene Symbol: Vps28
Description: VPS28, ESCRT-I subunit
Alias: vacuolar protein sorting-associated protein 28 homolog, vacuolar protein sorting 28 homolog
Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001;276:11735-42 pubmed
..We conclude that TSG101 and its interacting components are directly involved in endosomal sorting. ..
Bishop N, Horman A, Woodman P. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates. J Cell Biol. 2002;157:91-101 pubmed
..Similar defects are found in cells overexpressing Hrs, further emphasizing the links between class E protein function, receptor trafficking, and endosomal ubiquitination. ..
McDonald B, Martin Serrano J. Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase. Mol Biol Cell. 2008;19:754-63 pubmed
..However, accessibility to these lysines is prevented by the presence of the other ESCRT-I proteins. We show that VPS28 is a limiting factor, and consequently Tsg101 expression surplus to ESCRT-I function is vulnerable to degradation...
Stefani F, Zhang L, Taylor S, Donovan J, Rollinson S, Doyotte A, et al
. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Curr Biol. 2011;21:1245-50 pubmed publisher
..Mammalian ESCRT-I comprises TSG101, VPS28, VPS37A-D, and MVB12A/B...
Silvestri L, Ruthel G, Kallstrom G, Warfield K, Swenson D, Nelle T, et al
. Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction. J Infect Dis. 2007;196 Suppl 2:S264-70 pubmed
..particle-release assays, and confocal microscopy to investigate the potential role of ESCRT-1 proteins VPS4, VPS28, and VPS37B in EBOV budding...
Morita E, Sandrin V, Alam S, Eckert D, Gygi S, Sundquist W. Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell Host Microbe. 2007;2:41-53 pubmed
..All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37), and a fourth subunit of yeast ESCRT-I was recently identified (Mvb12p)...
Tsunematsu T, Yamauchi E, Shibata H, Maki M, Ohta T, Konishi H. Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications. Biochem Biophys Res Commun. 2010;399:232-7 pubmed publisher
..cargo protein from the plasma membrane to the endosomal vesicle, comprises a heterotetramer of TSG101 (Vps23), Vps28, Vps37 and MVB12 protein. In humans, the structurally similar subtypes MVB12A and MVB12B are subunits of ESCRT-I...
Boura E, Hurley J. Structural basis for membrane targeting by the MVB12-associated ?-prism domain of the human ESCRT-I MVB12 subunit. Proc Natl Acad Sci U S A. 2012;109:1901-6 pubmed publisher