Gene Symbol: Vcp
Description: valosin-containing protein
Alias: transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase
Species: rat
Products:     Vcp

Top Publications

  1. Meyer H, Wang Y, Warren G. Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4. EMBO J. 2002;21:5645-52 pubmed
    ..The UBA domain is required for the function of p47 in mitotic Golgi reassembly. Together, these data suggest that ubiquitin recognition is a common feature of p97-mediated reactions. ..
  2. Beuron F, Dreveny I, Yuan X, Pye V, McKeown C, Briggs L, et al. Conformational changes in the AAA ATPase p97-p47 adaptor complex. EMBO J. 2006;25:1967-76 pubmed
    The AAA+ATPase p97/VCP, helped by adaptor proteins, exerts its essential role in cellular events such as endoplasmic reticulum-associated protein degradation or the reassembly of Golgi, ER and the nuclear envelope after mitosis...
  3. Ye Y, Shibata Y, Kikkert M, van Voorden S, Wiertz E, Rapoport T. Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane. Proc Natl Acad Sci U S A. 2005;102:14132-8 pubmed
    ..and subsequent extraction from the membrane by the p97 ATPase [also called valosin-containing protein (VCP) or, in yeast, Cdc48]...
  4. Ye Y, Shibata Y, Yun C, Ron D, Rapoport T. A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol. Nature. 2004;429:841-7 pubmed
    ..Derlin-1 interacts with US11, a virally encoded ER protein that specifically targets MHC class I heavy chains for export from the ER, as well as with VIMP, a novel membrane protein that recruits the p97 ATPase and its cofactor. ..
  5. Kondo H, Rabouille C, Newman R, Levine T, Pappin D, Freemont P, et al. p47 is a cofactor for p97-mediated membrane fusion. Nature. 1997;388:75-8 pubmed
    ..As a homologue of p47 exists in budding yeast, this indicates that it might also be involved in other membrane fusion reactions catalysed by p97, such as karyogamy. ..
  6. Uchiyama K, Jokitalo E, Kano F, Murata M, Zhang X, Canas B, et al. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo. J Cell Biol. 2002;159:855-66 pubmed
    ..a novel essential factor for p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein, p135), and show that it binds to the p97/p47/syntaxin5 complex and ..
  7. Boyault C, Gilquin B, Zhang Y, Rybin V, Garman E, Meyer Klaucke W, et al. HDAC6-p97/VCP controlled polyubiquitin chain turnover. EMBO J. 2006;25:3357-66 pubmed
    ..We further show that HDAC6-interacting chaperone, p97/VCP, dissociates the HDAC6-ubiquitin complexes and counteracts the ability of HDAC6 to promote the accumulation of ..
  8. Watts G, Wymer J, Kovach M, Mehta S, Mumm S, Darvish D, et al. Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia is caused by mutant valosin-containing protein. Nat Genet. 2004;36:377-81 pubmed
    ..We found six missense mutations in the gene encoding valosin-containing protein (VCP, a member of the AAA-ATPase superfamily) exclusively in all 61 affected individuals...
  9. Wang Q, Li L, Ye Y. Regulation of retrotranslocation by p97-associated deubiquitinating enzyme ataxin-3. J Cell Biol. 2006;174:963-71 pubmed
    ..We present evidence that atx3 may promote p97-associated deubiquitination to facilitate the transfer of polypeptides from p97 to the proteasome. ..

More Information


  1. Mazzucchelli S, De Palma A, Riva M, D Urzo A, Pozzi C, Pastori V, et al. Proteomic and biochemical analyses unveil tight interaction of ataxin-3 with tubulin. Int J Biochem Cell Biol. 2009;41:2485-92 pubmed publisher
    ..These findings suggest an involvement of ataxin-3 in directing aggregated protein to aggresomes, and shed light on the mode of interaction among the different molecular partners participating in the process. ..
  2. Dai R, Li C. Valosin-containing protein is a multi-ubiquitin chain-targeting factor required in ubiquitin-proteasome degradation. Nat Cell Biol. 2001;3:740-4 pubmed
    ..We have shown previously that valosin-containing protein (VCP) physically and functionally targets the ubiquitinated nuclear factor kappaB inhibitor, IkappaBalpha to the ..
  3. Ye Y, Meyer H, Rapoport T. Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains. J Cell Biol. 2003;162:71-84 pubmed
  4. Mori Konya C, Kato N, Maeda R, Yasuda K, Higashimae N, Noguchi M, et al. p97/valosin-containing protein (VCP) is highly modulated by phosphorylation and acetylation. Genes Cells. 2009;14:483-97 pubmed publisher
    p97/valosin-containing protein (VCP) is a member of the AAA family proteins, which plays various important roles in cells by using its ATPase activity. But mechanism of regulating its ATPase activity is mostly unknown...
  5. Bartolomé F, Wu H, Burchell V, Preza E, Wray S, Mahoney C, et al. Pathogenic VCP mutations induce mitochondrial uncoupling and reduced ATP levels. Neuron. 2013;78:57-64 pubmed publisher
    Valosin-containing protein (VCP) is a highly expressed member of the type II AAA+ ATPase family...
  6. DeLaBarre B, Brunger A. Nucleotide dependent motion and mechanism of action of p97/VCP. J Mol Biol. 2005;347:437-52 pubmed
    ..One of the members of this family, the hexameric p97/valosin-containing protein p97/VCP, is essential for the dislocation of misfolded membrane proteins from the endoplasmic reticulum...
  7. Meyer H, Shorter J, Seemann J, Pappin D, Warren G. A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. EMBO J. 2000;19:2181-92 pubmed
    ..The fact that the binding of p47 and Ufd1/Npl4 is mutually exclusive suggests that these protein complexes act as adapters, directing a basic p97 activity into different cellular pathways. ..
  8. Roy L, Bergeron J, Lavoie C, Hendriks R, Gushue J, Fazel A, et al. Role of p97 and syntaxin 5 in the assembly of transitional endoplasmic reticulum. Mol Biol Cell. 2000;11:2529-42 pubmed
    ..Thus, p97 and syntaxin 5 regulate assembly of the smooth ER domain of tER and hence one of the earliest membrane differentiated components of the secretory pathway...
  9. Rabouille C, Kondo H, Newman R, Hui N, Freemont P, Warren G. Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro. Cell. 1998;92:603-10 pubmed
    ..p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to alpha-SNAP, which mediates the binding of NSF. ..
  10. Tresse E, Salomons F, Vesa J, Bott L, Kimonis V, Yao T, et al. VCP/p97 is essential for maturation of ubiquitin-containing autophagosomes and this function is impaired by mutations that cause IBMPFD. Autophagy. 2010;6:217-27 pubmed
    b>VCP (VCP/p97) is a ubiquitously expressed member of the AAA(+)-ATPase family of chaperone-like proteins that regulates numerous cellular processes including chromatin decondensation, homotypic membrane fusion and ubiquitin-dependent ..
  11. Kobayashi T, Tanaka K, Inoue K, Kakizuka A. Functional ATPase activity of p97/valosin-containing protein (VCP) is required for the quality control of endoplasmic reticulum in neuronally differentiated mammalian PC12 cells. J Biol Chem. 2002;277:47358-65 pubmed
    ..We previously identified p97/valosin-containing protein (VCP), an AAA ATPase with two conserved ATPase domains (D1 and D2), as an interacting partner of the Machado-Joseph ..
  12. Faouzi S, Medzihradszky K, Hefner C, Maher J, Correia M. Characterization of the physiological turnover of native and inactivated cytochromes P450 3A in cultured rat hepatocytes: a role for the cytosolic AAA ATPase p97?. Biochemistry. 2007;46:7793-803 pubmed
    ..These findings clearly reveal that native CYPs 3A undergo UPD and implicate a role for p97 in this process. ..
  13. Jerath N, Crockett C, Moore S, Shy M, Weihl C, Chou T, et al. Rare Manifestation of a c.290 C>T, p.Gly97Glu VCP Mutation. Case Rep Genet. 2015;2015:239167 pubmed publisher
    Introduction. The valosin-containing protein (VCP) regulates several distinct cellular processes. Consistent with this, VCP mutations manifest variable clinical phenotypes among and within families and are a diagnostic challenge. Methods...
  14. Gonzalez M, Feely S, Speziani F, Strickland A, Danzi M, Bacon C, et al. A novel mutation in VCP causes Charcot-Marie-Tooth Type 2 disease. Brain. 2014;137:2897-902 pubmed publisher
    Mutations in VCP have been reported to account for a spectrum of phenotypes that include inclusion body myopathy with Paget's disease of the bone and frontotemporal dementia, hereditary spastic paraplegia, and 1-2% of familial ..
  15. Zhang L, Ashendel C, Becker G, Morre D. Isolation and characterization of the principal ATPase associated with transitional endoplasmic reticulum of rat liver. J Cell Biol. 1994;127:1871-83 pubmed
    ..An ATPase with a monomer molecular weight of 100 kD by SDS-PAGE has been isolated from TER and designated as TER ATPase. The native TER ATPase has been characterized as a hexamer of six 100-kD subunits by gel filtration...
  16. Pécheur E, Martin I, Maier O, Bakowsky U, Ruysschaert J, Hoekstra D. Phospholipid species act as modulators in p97/p47-mediated fusion of Golgi membranes. Biochemistry. 2002;41:9813-23 pubmed
    ..We therefore propose that at physiological conditions PE-induced conformational changes in p97/p47 are relevant in triggering this activity. ..
  17. Hänzelmann P, Schindelin H. Structural Basis of ATP Hydrolysis and Intersubunit Signaling in the AAA+ ATPase p97. Structure. 2016;24:127-139 pubmed publisher
  18. Schuller J, Beck F, Lössl P, Heck A, Förster F. Nucleotide-dependent conformational changes of the AAA+ ATPase p97 revisited. FEBS Lett. 2016;590:595-604 pubmed publisher
    ..The largest conformational changes of p97 are 'swinging motions' of the N-terminal domains, which may enable segregation of ubiquitylated substrates from their environment. ..
  19. Mosbech A, Gibbs Seymour I, Kagias K, Thorslund T, Beli P, Povlsen L, et al. DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol. 2012;19:1084-92 pubmed publisher
    ..Our findings establish DVC1 as a DNA damage-targeting p97 adaptor that protects cells from deleterious consequences of replication blocks and suggest an important role of p97 in ubiquitin-dependent regulation of TLS...
  20. Koike M, Fukushi J, Ichinohe Y, Higashimae N, Fujishiro M, Sasaki C, et al. Valosin-containing protein (VCP) in novel feedback machinery between abnormal protein accumulation and transcriptional suppression. J Biol Chem. 2010;285:21736-49 pubmed publisher
    ..Genetic and biochemical analyses indicate that valosin-containing protein (VCP) is a crucial molecule in the pathogenesis of human neurodegenerative disorders...
  21. Olzmann J, Richter C, Kopito R. Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover. Proc Natl Acad Sci U S A. 2013;110:1345-50 pubmed publisher
    UBXD8 is a membrane-embedded recruitment factor for the p97/VCP segregase that has been previously linked to endoplasmic reticulum (ER)-associated degradation and to the control of triacylglycerol synthesis in the ER...
  22. Panier S, Boulton S. Double-strand break repair: 53BP1 comes into focus. Nat Rev Mol Cell Biol. 2014;15:7-18 pubmed publisher
    ..From these studies, a model is emerging in which 53BP1 recruitment requires the direct recognition of a DSB-specific histone code and its influence on pathway choice is mediated by mutual antagonism with breast cancer 1 (BRCA1). ..
  23. Tiwari A, Schuiki I, Zhang L, Allister E, Wheeler M, Volchuk A. SDF2L1 interacts with the ER-associated degradation machinery and retards the degradation of mutant proinsulin in pancreatic ?-cells. J Cell Sci. 2013;126:1962-8 pubmed publisher
    ..We suggest that SDF2L1 increases the time that misfolded proteins have to achieve a correctly folded conformation and thus that SDF2L1 can act as a buffer for substrate availability for ERAD in pancreatic ?-cells. ..
  24. Sato T, Sako Y, Sho M, Momohara M, Suico M, Shuto T, et al. STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein. Mol Cell. 2012;47:99-110 pubmed publisher
    ..Hence we postulate that STT3B-dependent posttranslational N-glycosylation is part of a triage-salvage system recognizing cryptic N-glycosylation sites of secretory proteins to preserve protein homeostasis. ..
  25. Panier S, Durocher D. Push back to respond better: regulatory inhibition of the DNA double-strand break response. Nat Rev Mol Cell Biol. 2013;14:661-72 pubmed publisher
  26. Dolan P, Jin Y, Hwang W, Johnson G. Decreases in valosin-containing protein result in increased levels of tau phosphorylated at Ser262/356. FEBS Lett. 2011;585:3424-9 pubmed publisher
    b>VCP/p97 is a multifunctional AAA+-ATPase involved in vesicle fusion, proteasomal degradation, and autophagy...
  27. Yeo B, Hong C, Chung K, Woo H, Kim K, Jung S, et al. Valosin-containing protein is a key mediator between autophagic cell death and apoptosis in adult hippocampal neural stem cells following insulin withdrawal. Mol Brain. 2016;9:31 pubmed publisher
    ..It is unknown how interconnection between ACD and apoptosis is mediated in HCN cells. Valosin-containing protein (VCP) is known to be essential for autophagosome maturation in mammalian cells...
  28. Flack J, Mieszczanek J, Novcic N, Bienz M. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell. 2017;67:181-193.e5 pubmed publisher
    ..Inactivation of TLE by UBR5-dependent ubiquitylation also involves VCP/p97, an AAA ATPase regulating the folding of various cellular substrates including ubiquitylated chromatin proteins...
  29. Pleasure I, Black M, Keen J. Valosin-containing protein, VCP, is a ubiquitous clathrin-binding protein. Nature. 1993;365:459-62 pubmed
    ..The 100K protein has been identified as valosin-containing protein (VCP; ref. 1), an early substrate for tyrosine phosphorylation on T-cell receptor activation...
  30. Banerjee S, Bartesaghi A, Merk A, Rao P, Bulfer S, Yan Y, et al. 2.3 Ã… resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science. 2016;351:871-5 pubmed publisher
  31. Chen S, Wu C, Lee Y, Tam K, Tsai Y, Liou J, et al. Caveolin-1 interacts with Derlin-1 and promotes ubiquitination and degradation of cyclooxygenase-2 via collaboration with p97 complex. J Biol Chem. 2013;288:33462-9 pubmed publisher
    ..Cav-1 may be a cofactor in the interaction of Derlin-1 and N-glycosylated COX-2 and may facilitate Derlin-1- and p97 complex-mediated COX-2 ubiquitination, retrotranslocation, and degradation. ..
  32. Wang H, Shih Y, Chen C, Chao H, Lee M, Hsueh Y. Valosin-containing protein and neurofibromin interact to regulate dendritic spine density. J Clin Invest. 2011;121:4820-37 pubmed publisher
    ..Although it is known to be caused by mutations in the gene encoding valosin-containing protein (VCP), the underlying disease mechanism remains elusive...
  33. Zhang H, Wang Q, Kajino K, Greene M. VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells. DNA Cell Biol. 2000;19:253-63 pubmed
    ..Here, we demonstrate that the BRCA1 protein physically associates with valosin-containing protein (VCP), a member of the ATPases associated with a variety of cellular activities (AAA) superfamily...
  34. Wang Q, Liu Y, Soetandyo N, Baek K, HEGDE R, Ye Y. A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation. Mol Cell. 2011;42:758-70 pubmed publisher
    ..Our results reveal a ubiquitin ligase-associated holdase that maintains polypeptide solubility to enhance protein quality control in mammalian cells. ..
  35. Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, et al. VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy. EMBO J. 2017;36:135-150 pubmed publisher
    ..Thus, our data reveal an important mechanism of how p97 maintains lysosomal homeostasis, and implicate the pathway as a modulator of degenerative diseases. ..
  36. Teng Y, Rezvani K, De Biasi M. UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP. Biochem Pharmacol. 2015;97:518-530 pubmed publisher
    ..UBXN2A also cross-talks with VCP/p97 and HSC70/HSP70 proteins in a complex where α3 is likely to be targeted by CHIP...
  37. McNeill H, Knebel A, Arthur J, Cuenda A, Cohen P. A novel UBA and UBX domain protein that binds polyubiquitin and VCP and is a substrate for SAPKs. Biochem J. 2004;384:391-400 pubmed
    ..SAKS1 bound ubiquitin tetramers and VCP (valosin-containing protein) in vitro via the UBA and UBX domains respectively...
  38. Kobayashi T, Manno A, Kakizuka A. Involvement of valosin-containing protein (VCP)/p97 in the formation and clearance of abnormal protein aggregates. Genes Cells. 2007;12:889-901 pubmed
    ..We have reported that valosin-containing protein (VCP) co-localizes with protein aggregates in patients' neurons and in cultured cells expressing diseased proteins...
  39. Blount J, Burr A, Denuc A, Marfany G, Todi S. Ubiquitin-specific protease 25 functions in Endoplasmic Reticulum-associated degradation. PLoS ONE. 2012;7:e36542 pubmed publisher
    ..Our data support a model where USP25 counteracts ubiquitination of ERAD substrates by the ubiquitin ligase HRD1, rescuing them from degradation by the proteasome. ..
  40. Livingstone M, Ruan H, Weiner J, Clauser K, Strack P, Jin S, et al. Valosin-containing protein phosphorylation at Ser784 in response to DNA damage. Cancer Res. 2005;65:7533-40 pubmed
    ..These include histone H2AX and DNA-PK itself. We identify here valosin-containing protein (VCP) as a novel substrate of DNA-PK and other PIKK family members...
  41. Noi K, Yamamoto D, Nishikori S, Arita Morioka K, Kato T, Ando T, et al. High-speed atomic force microscopic observation of ATP-dependent rotation of the AAA+ chaperone p97. Structure. 2013;21:1992-2002 pubmed publisher
    p97 (also called VCP and CDC-48) is an AAA+ chaperone, which consists of a substrate/cofactor-binding N domain and two ATPase domains (D1 and D2), and forms a homo-hexameric ring...
  42. Grelle G, Kostka S, Otto A, Kersten B, Genser K, Müller E, et al. Identification of VCP/p97, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners using membrane-based human proteome arrays. Mol Cell Proteomics. 2006;5:234-44 pubmed
  43. Ritz D, Vuk M, Kirchner P, Bug M, Schütz S, Hayer A, et al. Endolysosomal sorting of ubiquitylated caveolin-1 is regulated by VCP and UBXD1 and impaired by VCP disease mutations. Nat Cell Biol. 2011;13:1116-23 pubmed publisher
    The AAA-ATPase VCP (also known as p97) cooperates with distinct cofactors to process ubiquitylated proteins in different cellular pathways...
  44. Kadowaki H, Nagai A, Maruyama T, Takami Y, Satrimafitrah P, Kato H, et al. Pre-emptive Quality Control Protects the ER from Protein Overload via the Proximity of ERAD Components and SRP. Cell Rep. 2015;13:944-56 pubmed publisher
    ..These findings demonstrate that Derlins-mediated substrate-specific rerouting and Bag6- and p97-mediated effective degradation contribute to the maintenance of ER homeostasis without the need for translocation. ..
  45. Arita M, Wakita T, Shimizu H. Valosin-containing protein (VCP/p97) is required for poliovirus replication and is involved in cellular protein secretion pathway in poliovirus infection. J Virol. 2012;86:5541-53 pubmed publisher
    ..We identified valosin-containing protein (VCP/p97) as a host factor of PV replication required after viral protein synthesis, and its ATPase activity was ..
  46. Rao R, Poksay K, Castro Obregon S, Schilling B, Row R, Del Rio G, et al. Molecular components of a cell death pathway activated by endoplasmic reticulum stress. J Biol Chem. 2004;279:177-87 pubmed
    ..Our data identify two molecules, valosin-containing protein and apoptosis-linked gene-2 (ALG-2), that appear to play a role in mediating ER stress-induced cell death. ..
  47. Besche H, Haas W, Gygi S, Goldberg A. Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins. Biochemistry. 2009;48:2538-49 pubmed publisher
    ..By altering buffer conditions, we also purified by this approach complexes of the ATPase p97/VCP associated with its adaptor proteins Ufd1-Npl4, p47, SAKS1, and FAF1, all of which contain ubiquitin-binding motifs...
  48. Dai R, Chen E, Longo D, Gorbea C, Li C. Involvement of valosin-containing protein, an ATPase Co-purified with IkappaBalpha and 26 S proteasome, in ubiquitin-proteasome-mediated degradation of IkappaBalpha. J Biol Chem. 1998;273:3562-73 pubmed
    ..We identify valosin-containing protein (VCP), an AAA (ATPases associated with a variety of cellular activities) family member, that co-precipitates with ..
  49. Lizano P, Rashed E, Kang H, Dai H, Sui X, Yan L, et al. The valosin-containing protein promotes cardiac survival through the inducible isoform of nitric oxide synthase. Cardiovasc Res. 2013;99:685-93 pubmed publisher
    ..We tested both in vivo and in vitro the hypothesis that such an effector is the valosin-containing protein (VCP), an Akt substrate, which activates the transcription factor NF-?B, using a transgenic mouse with cardiac-specific ..
  50. Kaneko Y, Tamura K, Totsukawa G, Kondo H. Isolation of a point-mutated p47 lacking binding affinity to p97ATPase. FEBS Lett. 2010;584:3873-7 pubmed publisher
    ..In addition, mutation corresponding to the p47 F253S mutation in p37 and ufd1 also abolished their binding ability to p97. ..
  51. Ballar P, Zhong Y, Nagahama M, Tagaya M, Shen Y, Fang S. Identification of SVIP as an endogenous inhibitor of endoplasmic reticulum-associated degradation. J Biol Chem. 2007;282:33908-14 pubmed
    ..To test this hypothesis, we investigated the potential regulatory role of the small p97/VCP-interacting protein (SVIP) on the formation of the ERAD machinery that includes ubiquitin ligase gp78, AAA ATPase ..
  52. Laco M, Cortes L, Travis S, Paulson H, Rego A. Valosin-containing protein (VCP/p97) is an activator of wild-type ataxin-3. PLoS ONE. 2012;7:e43563 pubmed publisher
    ..with two proteins previously described to interact with ataxin-3, hHR23A and valosin-containing protein (VCP/p97). We confirmed ataxin-3 affinity for both hHR23A and VCP/p97...
  53. Bebeacua C, Forster A, McKeown C, Meyer H, Zhang X, Freemont P. Distinct conformations of the protein complex p97-Ufd1-Npl4 revealed by electron cryomicroscopy. Proc Natl Acad Sci U S A. 2012;109:1098-103 pubmed publisher
    ..Our results suggest a model for substrate remodeling by p97 and also explains how p97-Ufd1-Npl4 could form other complexes in a hierarchical model of p97-cofactor assembly. ..
  54. Ortak H, Cayli S, Tas U, Ocakli S, Sogut E, Demir H. Expression of p97/VCP and ubiquitin during postnatal development of the degenerating rat retina. J Mol Histol. 2012;43:17-25 pubmed publisher
    In this study, we aimed to investigate the distribution pattern of ubiquitin and p97/VCP in the rat retina during postnatal development...
  55. Cayli S, Erdemir F, Ocakli S, Ungor B, Kesici H, Yener T, et al. Interaction between Smad1 and p97/VCP in rat testis and epididymis during the postnatal development. Reprod Sci. 2012;19:190-201 pubmed publisher
    ..Valosin-containing protein (p97/VCP) is required for the degradation of some UPS substrates...
  56. Briggs L, Baldwin G, Miyata N, Kondo H, Zhang X, Freemont P. Analysis of nucleotide binding to P97 reveals the properties of a tandem AAA hexameric ATPase. J Biol Chem. 2008;283:13745-52 pubmed publisher
    ..ATPgammaS binding triggers a downstream cooperative conformational change of at least three monomers, which involves conserved arginine fingers and is necessary for ATP hydrolysis. ..
  57. Zhang S, Liu J, Kobayashi R, Tonks N. Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. J Biol Chem. 1999;274:17806-12 pubmed
    ..The PTPH1-D811A mutant trapped primarily a 97-kDa tyrosine-phosphorylated protein, which was determined to be VCP (also named p97 or yeast CDC48), from various cell lysates in vitro...
  58. Altier C, Garcia Caballero A, Simms B, You H, Chen L, Walcher J, et al. The Cav? subunit prevents RFP2-mediated ubiquitination and proteasomal degradation of L-type channels. Nat Neurosci. 2011;14:173-80 pubmed publisher
    ..Thus, Cav?? regulates the ubiquitination and stability of the calcium channel complex. ..
  59. Boyault C, Zhang Y, Fritah S, Caron C, Gilquin B, Kwon S, et al. HDAC6 controls major cell response pathways to cytotoxic accumulation of protein aggregates. Genes Dev. 2007;21:2172-81 pubmed
    ..HDAC6 therefore appears as a master regulator of the cell protective response to cytotoxic protein aggregate formation. ..
  60. Uchiyama K, Totsukawa G, Puhka M, Kaneko Y, Jokitalo E, Dreveny I, et al. p37 is a p97 adaptor required for Golgi and ER biogenesis in interphase and at the end of mitosis. Dev Cell. 2006;11:803-16 pubmed
    ..Interestingly, although VCIP135 is also required, its deubiquitinating activity is unnecessary for p97/p37-mediated activities. ..
  61. Cayli S, Ocakli S, Senel U, Eyerci N, Delibasi T. Role of p97/Valosin-containing protein (VCP) and Jab1/CSN5 in testicular ischaemia-reperfusion injury. J Mol Histol. 2016;47:91-100 pubmed publisher
    ..Furthermore, Jab1/CSN5 expression was gradually upregulated and p97/VCP expression was downregulated in IR injury according to western blotting and immunohistochemistry...
  62. Nakamura N, Harada K, Kato M, Hirose S. Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6. Exp Cell Res. 2014;328:207-16 pubmed publisher
    ..However, elimination of this processing had no evident effect on MARCH6 stabilization. These results suggest that USP19 is involved in the regulation of ERAD by controlling the stability of MARCH6 via deubiquitination...
  63. Acs K, Luijsterburg M, Ackermann L, Salomons F, Hoppe T, Dantuma N. The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks. Nat Struct Mol Biol. 2011;18:1345-50 pubmed publisher
    ..we show that RNF8-mediated ubiquitylation facilitates the recruitment of the AAA-ATPase valosin-containing protein (VCP, also known as p97) and its cofactor NPL4 to sites of double-strand breaks...
  64. Acharya P, Liao M, Engel J, Correia M. Liver cytochrome P450 3A endoplasmic reticulum-associated degradation: a major role for the p97 AAA ATPase in cytochrome P450 3A extraction into the cytosol. J Biol Chem. 2011;286:3815-28 pubmed publisher
    ..Intriguingly, the accumulated parent CYP3A was functionally active, indicating that p97 can regulate physiological CYP3A content and thus influence its clinically relevant function. ..