Gene Symbol: Vamp8
Description: vesicle-associated membrane protein 8
Alias: vesicle-associated membrane protein 8, EDB, VAMP-8, endobrevin
Species: rat
Products:     Vamp8

Top Publications

  1. Wong S, Zhang T, Xu Y, Subramaniam V, Griffiths G, Hong W. Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome. Mol Biol Cell. 1998;9:1549-63 pubmed
    ..The amino acid sequence of endobrevin has 32, 33, and 31% identity to those of synaptobrevin/VAMP-1, synaptobrevin/VAMP-2, and cellubrevin, ..
  2. Itakura E, Kishi Itakura C, Mizushima N. The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell. 2012;151:1256-69 pubmed publisher
    ..Stx17 interacts with SNAP-29 and the endosomal/lysosomal SNARE VAMP8. Depletion of Stx17 causes accumulation of autophagosomes without degradation...
  3. Polgar J, Chung S, Reed G. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion. Blood. 2002;100:1081-3 pubmed
    ..In contrast, rVAMP-2, which formed SNARE complexes in vitro, had no effect on platelet exocytosis. We conclude that VAMP-3 and VAMP-8 form SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion. ..
  4. Hubert V, Peschel A, Langer B, Groger M, Rees A, Kain R. LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes. Biol Open. 2016;5:1516-1529 pubmed publisher
    ..event is mediated by the interaction between the Qa-SNARE syntaxin-17 (STX17) on autophagosomes and the R-SNARE VAMP8 on lysosomes...
  5. Antonin W, Holroyd C, Fasshauer D, Pabst S, von Mollard G, Jahn R. A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO J. 2000;19:6453-64 pubmed publisher
    ..We report that syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8 form a complex that functions in the fusion of late endosomes...
  6. Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, et al. ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes. Nature. 2015;520:563-6 pubmed publisher
    ..factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8)...
  7. Antonin W, Fasshauer D, Becker S, Jahn R, Schneider T. Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs. Nat Struct Biol. 2002;9:107-11 pubmed
    ..crystal structure of an endosomal SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8...
  8. Williams D, Pessin J. Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes. J Cell Biol. 2008;180:375-87 pubmed publisher
    ..the initial biosynthetic entry of GLUT4 from the Golgi apparatus into the insulin-responsive vesicle compartment, VAMP8, for plasma membrane endocytosis and VAMP2 for sorting to the specialized insulin-responsive compartment after ..
  9. Messenger S, Jones E, Holthaus C, Thomas D, Cooley M, Byrne J, et al. Acute acinar pancreatitis blocks vesicle-associated membrane protein 8 (VAMP8)-dependent secretion, resulting in intracellular trypsin accumulation. J Biol Chem. 2017;292:7828-7839 pubmed publisher
    ..a first phase (0-2 min) mediated by VAMP2 and second (2-10 min) and third phases (10-30 min) mediated by VAMP8. Induction of acinar pancreatitis by supramaximal cholecystokinin (CCK-8) stimulation inhibits VAMP8-mediated mid- ..

More Information


  1. Pryor P, Mullock B, Bright N, Lindsay M, Gray S, Richardson S, et al. Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events. EMBO Rep. 2004;5:590-5 pubmed
    ..A trans-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes...
  2. Logan M, Lacy P, Odemuyiwa S, Steward M, Davoine F, Kita H, et al. A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils. Allergy. 2006;61:777-84 pubmed
    ..Our observations indicate for the first time a critical role for VAMP-7 in both eosinophil and neutrophil mediator release. ..
  3. Fasshauer D, Antonin W, Margittai M, Pabst S, Jahn R. Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties. J Biol Chem. 1999;274:15440-6 pubmed
    ..When the Q-SNAREs syntaxin 2, 3, and 4, and the R-SNARE endobrevin/VAMP 8 were used in various combinations, heat-resistant complexes were formed...
  4. Jones L, Moussa L, Fulcher M, Zhu Y, Hudson E, O Neal W, et al. VAMP8 is a vesicle SNARE that regulates mucin secretion in airway goblet cells. J Physiol. 2012;590:545-62 pubmed publisher
    ..In this study, we identify VAMP8 as one of the SNAREs regulating mucin granule exocytosis...
  5. Bilan F, Nacfer M, Fresquet F, Norez C, Melin P, Martin Berge A, et al. Endosomal SNARE proteins regulate CFTR activity and trafficking in epithelial cells. Exp Cell Res. 2008;314:2199-211 pubmed publisher
    ..Syntaxin 8 belongs to the endosomal SNARE complex which also contains syntaxin 7, vti1b and VAMP8. Here, we report that these four endosomal SNARE proteins physically and functionally interact with CFTR...
  6. Cosen Binker L, Binker M, Wang C, Hong W, Gaisano H. VAMP8 is the v-SNARE that mediates basolateral exocytosis in a mouse model of alcoholic pancreatitis. J Clin Invest. 2008;118:2535-51 pubmed publisher
    ..Here, we investigated the role of the zymogen granule (ZG) SNARE vesicle-associated membrane protein 8 (VAMP8) in mediating basolateral exocytosis...
  7. Antonin W, Holroyd C, Tikkanen R, Honing S, Jahn R. The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes. Mol Biol Cell. 2000;11:3289-98 pubmed
    b>Endobrevin/VAMP-8 is an R-SNARE localized to endosomes, but it is unknown in which intracellular fusion step it operates...
  8. Verges M, Havel R, Mostov K. A tubular endosomal fraction from rat liver: biochemical evidence of receptor sorting by default. Proc Natl Acad Sci U S A. 1999;96:10146-51 pubmed in proteins previously shown to be associated with recycling endosomes, such as rab 11, cellubrevin, and endobrevin, but relatively poor in early endosome antigen 1...
  9. Peters C, Michelson A, Flaumenhaft R. Granule exocytosis is required for platelet spreading: differential sorting of ?-granules expressing VAMP-7. Blood. 2012;120:199-206 pubmed publisher
  10. Low S, Marmorstein L, Miura M, Li X, Kudo N, Marmorstein A, et al. Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype. J Cell Sci. 2002;115:4545-53 pubmed
    ..In addition, syntaxin 4 and the v-SNARE endobrevin/VAMP-8 localize to this sub-tight junctional domain, which suggests that this is a region of preferred vesicle ..
  11. Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner R, Wang A, et al. Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion. EMBO J. 2016;35:1810-21 pubmed publisher
    ..a proteomics approach using a PKCB knockout mast cell model and found that the key mast cell secretory protein VAMP8 becomes phosphorylated by PKC at multiple residues in the SNARE domain...
  12. Sander L, Frank S, Bolat S, Blank U, Galli T, Bigalke H, et al. Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells. Eur J Immunol. 2008;38:855-63 pubmed publisher
    ..In summary, our data show that mature human MC express a specific pattern of SNARE and that VAMP-7 and VAMP-8, but not VAMP-2, are required for rapid degranulation...
  13. Lippert U, Ferrari D, Jahn R. Endobrevin/VAMP8 mediates exocytotic release of hexosaminidase from rat basophilic leukaemia cells. FEBS Lett. 2007;581:3479-84 pubmed
    ..Of a complete set of recombinant mammalian R-SNAREs, only vesicle associated membrane protein (VAMP8)/endobrevin consistently blocked hexosaminidase release, which was also insensitive to treatment with clostridial ..
  14. Weng N, Thomas D, Groblewski G. Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion. J Biol Chem. 2007;282:9635-45 pubmed
    Previous studies have demonstrated roles for vesicle-associated membrane protein 2 (VAMP 2) and VAMP 8 in Ca(2+)-regulated pancreatic acinar cell secretion, however, their coordinated function in the secretory pathway has not been ..
  15. Hasan N, Corbin D, Hu C. Fusogenic pairings of vesicle-associated membrane proteins (VAMPs) and plasma membrane t-SNAREs--VAMP5 as the exception. PLoS ONE. 2010;5:e14238 pubmed publisher
    ..These data indicate that VAMPs have differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs. ..
  16. Steegmaier M, Lee K, Prekeris R, Scheller R. SNARE protein trafficking in polarized MDCK cells. Traffic. 2000;1:553-60 pubmed
    ..Here we show that VAMP8/endobrevin cycles through the apical but not through the basolateral plasma membrane...
  17. Sakiyama H, Tadokoro S, Nakanishi M, Hirashima N. Membrane fusion between liposomes containing SNARE proteins involved in mast cell exocytosis. Inflamm Res. 2009;58:139-42 pubmed publisher
    ..The core components of SNAP23, syntaxin-3, and VAMP-8 appear to be minimal machinery to induce membrane fusion, while VAMP-7 appears to be unessential for membrane fusion. ..
  18. Low S, Li X, Miura M, Kudo N, Quiñones B, Weimbs T. Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells. Dev Cell. 2003;4:753-9 pubmed
    ..Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells...