Gene Symbol: Tsg101
Description: tumor susceptibility 101
Alias: tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, Tumor supressor
Species: rat
Products:     Tsg101

Top Publications

  1. Turpin E, Dalle B, De Roquancourt A, Plassa L, Marty M, Janin A, et al. Stress-induced aberrant splicing of TSG101: association to high tumor grade and p53 status in breast cancers. Oncogene. 1999;18:7834-7 pubmed
    The TSG101 gene, identified through insertional mutagenesis, is localized in a region that exhibits LOH in human cancers, suggesting that TSG101 might be a tumor suppressor gene...
  2. Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001;276:11735-42 pubmed
    ..We have identified the mammalian orthologue of Vps28p as a 221- amino acid cytosolic protein that interacts with TSG101/mammalian VPS23 to form part of a multiprotein complex...
  3. Malerød L, Stuffers S, Brech A, Stenmark H. Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation. Traffic. 2007;8:1617-29 pubmed
    ..kinase1/2 downstream of the EGF-activated receptor were sustained by depletion of Hrs or the ESCRT-I subunit Tsg101. In contrast, this was not the case when Vps22 was depleted...
  4. Klaes R, Kloor M, Willeke F, Melsheimer P, von Knebel Doeberitz M, Ridder R. Significant increase of a specific variant TSG101 transcript during the progression of cervical neoplasia. Eur J Cancer. 1999;35:733-7 pubmed
    The human tumour susceptibility gene TSG101 has recently been identified on chromosomal locus 11p15.1-15.2 which is frequently affected by genetic alterations in neoplastic lesions of the uterine cervix...
  5. Lo Y, Chen T, Chen S, Chao C. Aberrant expression of TSG101 in Taiwan Chinese breast cancer. Breast Cancer Res Treat. 2000;60:259-66 pubmed
    Functional inactivation of the tsg101 gene in mouse fibroblasts results in cell transformation and the ability to form metastatic tumors in nude mice. The human tsg101 gene was mapped to chromosome 11q15...
  6. Tsunematsu T, Yamauchi E, Shibata H, Maki M, Ohta T, Konishi H. Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications. Biochem Biophys Res Commun. 2010;399:232-7 pubmed publisher
    ..of ubiquitinated cargo protein from the plasma membrane to the endosomal vesicle, comprises a heterotetramer of TSG101 (Vps23), Vps28, Vps37 and MVB12 protein...
  7. Bishop N, Horman A, Woodman P. Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates. J Cell Biol. 2002;157:91-101 pubmed
    ..Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-..
  8. Leithe E, Kjenseth A, Sirnes S, Stenmark H, Brech A, Rivedal E. Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101. J Cell Sci. 2009;122:3883-93 pubmed publisher
    ..proteins Hrs (hepatocyte growth factor-regulated tyrosine kinase substrate; also known as Hgs) and Tsg101 (tumor susceptibility gene 101)...
  9. Kim B, Olzmann J, Barsh G, Chin L, Li L. Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking. Mol Biol Cell. 2007;18:1129-42 pubmed
    ..Here, we report the identification of TSG101, a key component of the endosomal sorting complex required for transport (ESCRT)-I, as a specific Mahogunin ..

More Information


  1. Young T, Rosen D, Mei F, Li N, Liu J, Wang X, et al. Up-regulation of tumor susceptibility gene 101 conveys poor prognosis through suppression of p21 expression in ovarian cancer. Clin Cancer Res. 2007;13:3848-54 pubmed
    The function of tumor susceptibility gene 101 (TSG101) in ovarian carcinogenesis is largely unexplored...
  2. Zhou H, Yuen P, Pisitkun T, Gonzales P, Yasuda H, Dear J, et al. Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int. 2006;69:1471-6 pubmed
    ..These urine collection, storage, and processing conditions may be useful for future biomarker discovery efforts. ..
  3. Silvestri L, Ruthel G, Kallstrom G, Warfield K, Swenson D, Nelle T, et al. Involvement of vacuolar protein sorting pathway in Ebola virus release independent of TSG101 interaction. J Infect Dis. 2007;196 Suppl 2:S264-70 pubmed
    ..EBOV virus matrix protein VP40 recruits TSG101, an ESCRT-1 (host cell endosomal sorting complex required for transport-1) complex protein in the vacuolar protein ..
  4. Blot V, Perugi F, Gay B, Pr vost M, Briant L, Tangy F, et al. Nedd4.1-mediated ubiquitination and subsequent recruitment of Tsg101 ensure HTLV-1 Gag trafficking towards the multivesicular body pathway prior to virus budding. J Cell Sci. 2004;117:2357-67 pubmed publisher
    ..ligase from the Nedd4 family, whereas those of the human immunodeficiency virus interact through a PTAP motif with Tsg101, a protein of the ESCRT-1 complex...
  5. Malbert Colas L, Nicolas G, Galand C, Lecomte M, Dhermy D. Identification of new partners of the epithelial sodium channel alpha subunit. C R Biol. 2003;326:615-24 pubmed
    ..screenings, we identified new potential partners of alpha-ENaC: WWP1 (E3 ubiquitin-ligase protein), UBC9 and TSG101 (E2 ubiquitin/SUMO-conjugating enzymes) and confirmed these interactions in GST pull-down assays...
  6. Rountree M, Bachman K, Baylin S. DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat Genet. 2000;25:269-77 pubmed
    ..DMAP1 has intrinsic transcription repressive activity, and binds to the transcriptional co-repressor TSG101. DMAP1 is targeted to replication foci through interaction with the far N terminus of DNMT1 throughout S phase, ..
  7. Stefani F, Zhang L, Taylor S, Donovan J, Rollinson S, Doyotte A, et al. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Curr Biol. 2011;21:1245-50 pubmed publisher
    ..Mammalian ESCRT-I comprises TSG101, VPS28, VPS37A-D, and MVB12A/B...
  8. Zheng Y, Campbell E, Lucocq J, Riches A, Powis S. Monitoring the Rab27 associated exosome pathway using nanoparticle tracking analysis. Exp Cell Res. 2013;319:1706-13 pubmed publisher
    ..These data show that nanoparticle tracking analysis can be used effectively and rapidly to monitor the disruption of exosome secretion. ..
  9. Tsunemi T, Hamada K, Krainc D. ATP13A2/PARK9 regulates secretion of exosomes and α-synuclein. J Neurosci. 2014;34:15281-7 pubmed publisher
    ..Together, these data suggest the involvement of PARK9 in the biogenesis of exosomes and α-syn secretion and raise a possibility that disruption of these pathways in patients with KRS contributes to the disease pathogenesis. ..
  10. Burgdorf S, Leister P, Scheidtmann K. TSG101 interacts with apoptosis-antagonizing transcription factor and enhances androgen receptor-mediated transcription by promoting its monoubiquitination. J Biol Chem. 2004;279:17524-34 pubmed
    ..In search of novel interaction partners of AATF, we identified the tumor susceptibility gene product TSG101, which had also been recognized as a co-regulator of nuclear hormone receptors...
  11. McDonald B, Martin Serrano J. Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase. Mol Biol Cell. 2008;19:754-63 pubmed
    As part of the endosomal sorting complex required for transport (ESCRT) machinery, Tsg101 is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis...
  12. Kantamneni S, Holman D, Wilkinson K, Corrêa S, Feligioni M, Ogden S, et al. GISP binding to TSG101 increases GABA receptor stability by down-regulating ESCRT-mediated lysosomal degradation. J Neurochem. 2008;107:86-95 pubmed publisher
    ..Here, we show that the protein tumour susceptibility gene 101 (TSG101), an integral component of the endosomal sorting machinery that targets membrane proteins for lysosomal ..
  13. Boura E, Hurley J. Structural basis for membrane targeting by the MVB12-associated ?-prism domain of the human ESCRT-I MVB12 subunit. Proc Natl Acad Sci U S A. 2012;109:1901-6 pubmed publisher
  14. Bache K, Stuffers S, Malerød L, Slagsvold T, Raiborg C, Lechardeur D, et al. The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor. Mol Biol Cell. 2006;17:2513-23 pubmed
    ..Here, we have compared the importance of the ESCRT-I subunit tumor susceptibility gene 101 (Tsg101) and the ESCRT-III subunit hVps24/CHMP3 for endosomal functions and receptor signaling...
  15. Okumura M, Ichioka F, Kobayashi R, Suzuki H, Yoshida H, Shibata H, et al. Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101. Biochem Biophys Res Commun. 2009;386:237-41 pubmed publisher
    Alix and TSG101, known to physically interact with each other, have Pro-rich regions that are bound by ALG-2 Ca2+-dependently...
  16. O Boyle J, Proctor M, Fong K, Lin W, Miller D, Muller C. Role of TSG101 in uterine cervix cancer. Gynecol Oncol. 1999;75:401-5 pubmed
    b>TSG101 was first described as a possible tumor suppressor gene in breast cancer...
  17. Garrus J, von Schwedler U, Pornillos O, Morham S, Zavitz K, Wang H, et al. Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001;107:55-65 pubmed
    Like other enveloped viruses, HIV-1 uses cellular machinery to bud from infected cells. We now show that Tsg101 protein, which functions in vacuolar protein sorting (Vps), is required for HIV-1 budding...
  18. Morita E, Sandrin V, Alam S, Eckert D, Gygi S, Sundquist W. Identification of human MVB12 proteins as ESCRT-I subunits that function in HIV budding. Cell Host Microbe. 2007;2:41-53 pubmed
    ..All ESCRT-I complexes contain three common subunits (TSG101, VPS28, and VPS37), and a fourth subunit of yeast ESCRT-I was recently identified (Mvb12p)...
  19. Nabhan J, Hu R, Oh R, Cohen S, Lu Q. Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Proc Natl Acad Sci U S A. 2012;109:4146-51 pubmed publisher
    ..Budding viruses exploit the TSG101 protein and endosomal sorting complex required for transport (ESCRT) machinery used for MVB formation to mediate ..
  20. Rusten T, Stenmark H. How do ESCRT proteins control autophagy?. J Cell Sci. 2009;122:2179-83 pubmed publisher
    ..We propose that the involvement of ESCRT proteins in the fusion of autophagosomes with the endolysosomal system is the most plausible model. ..
  21. Oh K, Stanton M, West W, Todd G, Wagner K. Tsg101 is upregulated in a subset of invasive human breast cancers and its targeted overexpression in transgenic mice reveals weak oncogenic properties for mammary cancer initiation. Oncogene. 2007;26:5950-9 pubmed
    Previous studies reported that the Tumor Susceptibility Gene 101 (TSG101) is upregulated in selected human malignancies, and the expression of exogenous Tsg101 was suggested to transform immortalized fibroblasts in culture...
  22. Oh H, Mammucari C, Nenci A, Cabodi S, Cohen S, Dotto G. Negative regulation of cell growth and differentiation by TSG101 through association with p21(Cip1/WAF1). Proc Natl Acad Sci U S A. 2002;99:5430-5 pubmed
    b>TSG101 was discovered in a screen for tumor susceptibility genes and has since been shown to have a multiplicity of biological effects. However, the basis for TSG101's ability to regulate cell growth has not been elucidated...
  23. Langelier C, von Schwedler U, Fisher R, De Domenico I, White P, Hill C, et al. Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. J Virol. 2006;80:9465-80 pubmed
    ..N-terminal GRAM-like ubiquitin-binding in EAP45 (GLUE) domain, both EAP45 and EAP30 bound the C-terminal domain of TSG101/ESCRT-I, and EAP20 bound the N-terminal half of CHMP6/ESCRT-III...
  24. Puertollano R. Interactions of TOM1L1 with the multivesicular body sorting machinery. J Biol Chem. 2005;280:9258-64 pubmed
    ..substrate), whereas a PTAP motif, located between the VHS and GAT domain of Tom1L1, is responsible for binding to TSG101 (tumor susceptibility gene 101)...
  25. Li L, Li X, Francke U, Cohen S. The TSG101 tumor susceptibility gene is located in chromosome 11 band p15 and is mutated in human breast cancer. Cell. 1997;88:143-54 pubmed
    Recent work has identified a mouse gene (tsg101) whose inactivation in fibroblasts results in cellular transformation and the ability to produce metastatic tumors in nude mice...
  26. Colombo M, Moita C, van Niel G, Kowal J, Vigneron J, Benaroch P, et al. Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles. J Cell Sci. 2013;126:5553-65 pubmed publisher
    ..Silencing of HRS, STAM1 or TSG101 reduced the secretion of EV-associated CD63 and MHC II but each gene altered differently the size and/or protein ..
  27. Sun Z, Pan J, Bubley G, Balk S. Frequent abnormalities of TSG101 transcripts in human prostate cancer. Oncogene. 1997;15:3121-5 pubmed
    b>TSG101 has been identified as a candidate tumor suppressor gene and abnormal transcripts have been identified in a substantial fraction of breast cancers...
  28. Chang J, Su T, Wei H, Wang J, Chen Y, Chang C, et al. Analysis of TSG101 tumour susceptibility gene transcripts in cervical and endometrial cancers. Br J Cancer. 1999;79:445-50 pubmed
    ..Recent studies have localized the TSG101 gene in this region, and also demonstrated a high frequency of abnormalities of this gene in human breast cancer...
  29. Stuchell M, Garrus J, Muller B, Stray K, Ghaffarian S, McKinnon R, et al. The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding. J Biol Chem. 2004;279:36059-71 pubmed
    ..budding requires an interaction between the PTAP late domain in the viral p6(Gag) protein and the cellular protein TSG101. In yeast, Vps23p/TSG101 binds both Vps28p and Vps37p to form the soluble ESCRT-I complex, which functions in ..
  30. Amit I, Yakir L, Katz M, Zwang Y, Marmor M, Citri A, et al. Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes Dev. 2004;18:1737-52 pubmed
    The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101...
  31. Torres G, Bitran M, Huidobro Toro J. Co-release of neuropeptide Y (NPY) and noradrenaline from the sympathetic nerve terminals supplying the rat vas deferens; influence of calcium and the stimulation intensity. Neurosci Lett. 1992;148:39-42 pubmed
    ..In the absence of external Ca2+, neurotransmission was blocked and co-release of ir-NPY and NA was prevented. ..
  32. Felten A, Leister P, Burgdorf S, Uhlmann L, Scheidtmann K. Characterization of rat BLOS2/Ceap, a putative yeast She3 homolog, as interaction partner of apoptosis antagonizing transcription factor/Che-1. Biol Chem. 2007;388:569-82 pubmed
    ..We propose that BLOS2 functions as an adapter in processes such as protein and vesicle processing and transport, and perhaps transcription. ..