Gene Symbol: Trhde
Description: thyrotropin-releasing hormone degrading enzyme
Alias: PAP-II, thyrotropin-releasing hormone-degrading ectoenzyme, TRH-DE, TRH-degrading ectoenzyme, TRH-specific aminopeptidase, pyroglutamyl-peptidase II, thyroliberinase
Schauder B, Schomburg L, Kohrle J, Bauer K. Cloning of a cDNA encoding an ectoenzyme that degrades thyrotropin-releasing hormone. Proc Natl Acad Sci U S A. 1994;91:9534-8 pubmed
..Finally, transient transfection of COS-7 cells with this cDNA led to the expression of an active ectopeptidase that displayed the characteristics of the TRH-degrading ectoenzyme. ..
Papadopoulos T, Heuer H, Bauer K. Analysis of the thyrotropin-releasing hormone-degrading ectoenzyme by site-directed mutagenesis of cysteine residues. Cys68 is involved in disulfide-linked dimerization. Eur J Biochem. 2000;267:2617-23 pubmed
..Covalent dimerization of the subunits, however, does not seem to be essential for efficient biosynthesis, enzymatic activity and trafficking to the cell surface. ..
Schmitmeier S, Thole H, Bader A, Bauer K. Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin. Eur J Biochem. 2002;269:1278-86 pubmed
..These data strongly support the hypothesis that the TRH-DEs are all derived from the same gene, whereby the serum enzyme is generated by proteolytic cleavage of the particulate liver enzyme. ..
Chávez Gutiérrez L, Matta Camacho E, Osuna J, Horjales E, Joseph Bravo P, Maigret B, et al
. Homology modeling and site-directed mutagenesis of pyroglutamyl peptidase II. Insights into omega-versus aminopeptidase specificity in the M1 family. J Biol Chem. 2006;281:18581-90 pubmed
b>Pyroglutamyl peptidase II (PPII), a highly specific membrane-bound omegapeptidase, removes N-terminal pyroglutamyl from thyrotropin-releasing hormone (<Glu-His-Pro-NH(2)), inactivating the peptide in the extracellular space...
Sanchez E, Vargas M, Singru P, Pascual I, Romero F, Fekete C, et al
. Tanycyte pyroglutamyl peptidase II contributes to regulation of the hypothalamic-pituitary-thyroid axis through glial-axonal associations in the median eminence. Endocrinology. 2009;150:2283-91 pubmed publisher
b>Pyroglutamyl peptidase II (PPII), a highly specific membrane-bound metallopeptidase that inactivates TRH in the extracellular space, is tightly regulated by thyroid hormone in cells of the anterior pituitary...
Konishi H, Ogawa T, Kawahara S, Matsumoto S, Kiyama H. Continuous stress-induced dopamine dysregulation augments PAP-I and PAP-II expression in melanotrophs of the pituitary gland. Biochem Biophys Res Commun. 2011;407:7-12 pubmed publisher
..Because the PAP family members are implicated in cell survival and proliferation, PAP-I and PAP-II secreted from melanotrophs may function to sustain homeostasis of the IL under CS conditions in an autocrine or a paracrine manner. ..
Lazcano I, Uribe R, Martínez Chávez E, Vargas M, Matziari M, Joseph Bravo P, et al
. Pyroglutamyl peptidase II inhibition enhances the analeptic effect of thyrotropin-releasing hormone in the rat medial septum. J Pharmacol Exp Ther. 2012;342:222-31 pubmed publisher
..It is hydrolyzed in vitro by pyroglutamyl peptidase II (PPII), a narrow specificity ectoenzyme with a preferential localization in the brain, but evidence that ..
Alvarez Salas E, Aceves C, Anguiano B, Uribe R, García Luna C, Sanchez E, et al
. Food-restricted and dehydrated-induced anorexic rats present differential TRH expression in anterior and caudal PVN. Role of type 2 deiodinase and pyroglutamyl aminopeptidase II. Endocrinology. 2012;153:4067-76 pubmed publisher
..Increased type 2 deiodinase activity seemed responsible for low medial PVN TRH synthesis, whereas increased medial basal hypothalamic pyroglutamyl-aminopeptidase II activity in DIA rats might counteract their high TRH release. ..
Lazcano I, Cabral A, Uribe R, Jaimes Hoy L, Perello M, Joseph Bravo P, et al
. Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of the Mediobasal Hypothalamus of Male Adult Rats. Endocrinology. 2015;156:2713-23 pubmed publisher
..Tanycytes express pyroglutamyl peptidase II (PPII), the TRH-degrading ectoenzyme that controls the amount of TRH that reaches the anterior pituitary...