Tomm20

Summary

Gene Symbol: Tomm20
Description: translocase of outer mitochondrial membrane 20
Alias: mitochondrial import receptor subunit TOM20 homolog, mitochondrial 20 kDa outer membrane protein, outer mitochondrial membrane receptor Tom20, outer mitochondrial membrane receptor rTOM20, translocase of outer mitochondrial membrane 20 homolog
Species: rat
Products:     Tomm20

Top Publications

  1. Abe Y, Shodai T, Muto T, Mihara K, Torii H, Nishikawa S, et al. Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20. Cell. 2000;100:551-60 pubmed
    ..Although the positive charges of the presequence are essential for import ability, presequence binding to Tom20 is mediated mainly by hydrophobic rather than ionic interactions. ..
  2. Yamamoto H, Itoh N, Kawano S, Yatsukawa Y, Momose T, Makio T, et al. Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria. Proc Natl Acad Sci U S A. 2011;108:91-6 pubmed publisher
    ..Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency...
  3. Di Maio R, Barrett P, Hoffman E, Barrett C, Zharikov A, Borah A, et al. ?-Synuclein binds to TOM20 and inhibits mitochondrial protein import in Parkinson's disease. Sci Transl Med. 2016;8:342ra78 pubmed publisher
    ..This study characterizes a pathogenic mechanism in PD, identifies toxic species of wild-type ?-synuclein, and reveals potential new therapeutic strategies for neuroprotection. ..
  4. Igura M, Ose T, Obita T, Sato C, Maenaka K, Endo T, et al. Crystallization and preliminary X-ray analysis of mitochondrial presequence receptor Tom20 in complexes with a presequence from aldehyde dehydrogenase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:514-7 pubmed
    ..1 and 1.9 A. Crystal dehydration and subsequent annealing was essential to obtain good diffraction data for the 2.1 A crystal form. ..
  5. Yano M, Terada K, Mori M. AIP is a mitochondrial import mediator that binds to both import receptor Tom20 and preproteins. J Cell Biol. 2003;163:45-56 pubmed
    ..These results suggest that AIP functions as a cytosolic factor that mediates preprotein import into mitochondria. ..
  6. Wenz L, OpaliÅ„ski Å, Wiedemann N, Becker T. Cooperation of protein machineries in mitochondrial protein sorting. Biochim Biophys Acta. 2015;1853:1119-29 pubmed publisher
    ..Therefore, we propose that the protein transport systems are part of a complicated protein network for mitochondrial biogenesis. ..
  7. Korrodi Gregório L, Vieira S, Esteves S, Silva J, Freitas M, Brauns A, et al. TCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network. Biol Open. 2013;2:453-65 pubmed publisher
    ..In essence TCTEX1D4/PPP1C complex appears to be involved in microtubule dynamics, sperm motility, acrosome reaction and in the regulation of the blood-testis barrier. ..
  8. Craig E, Chesley A, Hood D. Thyroid hormone modifies mitochondrial phenotype by increasing protein import without altering degradation. Am J Physiol. 1998;275:C1508-15 pubmed
  9. Kanaji S, Iwahashi J, Kida Y, Sakaguchi M, Mihara K. Characterization of the signal that directs Tom20 to the mitochondrial outer membrane. J Cell Biol. 2000;151:277-88 pubmed

More Information

Publications28

  1. Schulz C, Schendzielorz A, Rehling P. Unlocking the presequence import pathway. Trends Cell Biol. 2015;25:265-75 pubmed publisher
    ..Here, we review recent advances in understanding of the mechanisms underlying protein transport along the presequence pathway. ..
  2. Mashimo M, Kato J, Moss J. ADP-ribosyl-acceptor hydrolase 3 regulates poly (ADP-ribose) degradation and cell death during oxidative stress. Proc Natl Acad Sci U S A. 2013;110:18964-9 pubmed publisher
    ..Thus, PARG release of PAR attached to nuclear proteins, followed by ARH3 cleavage of PAR, is essential in regulating PAR-dependent AIF release from mitochondria and parthanatos. ..
  3. Takahashi M, Chesley A, Freyssenet D, Hood D. Contractile activity-induced adaptations in the mitochondrial protein import system. Am J Physiol. 1998;274:C1380-7 pubmed
    ..This increase in protein import is likely an important adaptation in the overall process of mitochondrial biogenesis. ..
  4. Ellenrieder L, MÃ¥rtensson C, Becker T. Biogenesis of mitochondrial outer membrane proteins, problems and diseases. Biol Chem. 2015;396:1199-213 pubmed publisher
    ..We summarize current models and open questions of biogenesis of mitochondrial outer membrane proteins and discuss the impact of malfunctions of protein sorting on the development of diseases. ..
  5. Valentijn A, Metcalfe A, Kott J, Streuli C, Gilmore A. Spatial and temporal changes in Bax subcellular localization during anoikis. J Cell Biol. 2003;162:599-612 pubmed
    ..Our data suggest that Bax regulation occurs at several levels, with formation of clusters a late event, and with critical changes determining cell fate occurring earlier. ..
  6. Singh K, Hood D. Effect of denervation-induced muscle disuse on mitochondrial protein import. Am J Physiol Cell Physiol. 2011;300:C138-45 pubmed publisher
    ..e., increased ROS production and decreased respiration) during chronic disuse, which likely contribute to the activation of degradative pathways leading to muscle atrophy. ..
  7. Saitoh T, Igura M, Miyazaki Y, Ose T, Maita N, Kohda D. Crystallographic snapshots of Tom20-mitochondrial presequence interactions with disulfide-stabilized peptides. Biochemistry. 2011;50:5487-96 pubmed publisher
  8. Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, et al. Autophagosomes form at ER-mitochondria contact sites. Nature. 2013;495:389-93 pubmed publisher
    ..These results provide new insight into organelle biogenesis by demonstrating that the ER-mitochondria contact site is important in autophagosome formation...
  9. Yano M, Terada K, Mori M. Mitochondrial import receptors Tom20 and Tom22 have chaperone-like activity. J Biol Chem. 2004;279:10808-13 pubmed
    ..These results suggest that the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface. ..
  10. Komuro Y, Miyashita N, Mori T, Muneyuki E, Saitoh T, Kohda D, et al. Energetics of the presequence-binding poses in mitochondrial protein import through Tom20. J Phys Chem B. 2013;117:2864-71 pubmed publisher
    ..The REMD simulations thus provide insights into the energetics of the multiple-binding forms and help to detail the progressive conformational states in the dynamic-equilibrium model based on the experimental data. ..
  11. Cleland M, Norris K, Karbowski M, Wang C, Suen D, Jiao S, et al. Bcl-2 family interaction with the mitochondrial morphogenesis machinery. Cell Death Differ. 2011;18:235-47 pubmed publisher
  12. Saitoh T, Igura M, Obita T, Ose T, Kojima R, Maenaka K, et al. Tom20 recognizes mitochondrial presequences through dynamic equilibrium among multiple bound states. EMBO J. 2007;26:4777-87 pubmed
    ..We suggest that the dynamic, multiple-mode interaction is the molecular mechanism facilitating the broadly selective specificity of the Tom20 receptor toward diverse mitochondrial presequences. ..
  13. Zara V, Ferramosca A, Papatheodorou P, Palmieri F, Rassow J. Import of rat mitochondrial citrate carrier (CIC) at increasing salt concentrations promotes presequence binding to import receptor Tom20 and inhibits membrane translocation. J Cell Sci. 2005;118:3985-95 pubmed
    ..We conclude that presequences can only act as mediators of mitochondrial protein import if they allow rapid release from import receptor sites. Release from receptors sites may be rate-limiting in translocation. ..
  14. Wurm C, Neumann D, Lauterbach M, Harke B, Egner A, Hell S, et al. Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient. Proc Natl Acad Sci U S A. 2011;108:13546-51 pubmed publisher
    ..We conclude that the nanoscale distribution of the TOM complex is finely adjusted to the cellular conditions, resulting in distribution gradients both within single cells and between adjacent cells. ..
  15. Richter V, Palmer C, Osellame L, Singh A, Elgass K, Stroud D, et al. Structural and functional analysis of MiD51, a dynamin receptor required for mitochondrial fission. J Cell Biol. 2014;204:477-86 pubmed publisher
    ..MiD51 foci are also dependent on the presence of Drp1, and after scission they are distributed to daughter organelles, supporting the involvement of MiD51 in the fission apparatus...
  16. Höhr A, Straub S, Warscheid B, Becker T, Wiedemann N. Assembly of β-barrel proteins in the mitochondrial outer membrane. Biochim Biophys Acta. 2015;1853:74-88 pubmed publisher
  17. Guarani V, McNeill E, Paulo J, Huttlin E, Fröhlich F, Gygi S, et al. QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology. elife. 2015;4: pubmed publisher
    ..Collectively, our work uncovers a previously unrecognized subunit of the MICOS complex, necessary for CJ integrity, cristae morphology, and mitochondrial function and provides a resource for further analysis of MICOS architecture. ..
  18. Zhu Y, Swanson B, Wang M, Hildeman D, Schaefer B, Liu X, et al. Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells. Proc Natl Acad Sci U S A. 2004;101:7681-6 pubmed
    ..Our results indicate that, in T cells, Bim function is regulated by interaction with Bcl-2 family members on mitochondria rather than by sequestration to the microtubules. ..
  19. Iwashita S, Tsuchida M, Tsukuda M, Yamashita Y, Emi Y, Kida Y, et al. Multiple organelle-targeting signals in the N-terminal portion of peroxisomal membrane protein PMP70. J Biochem. 2010;147:581-90 pubmed publisher
    ..Cooperation of the organelle-targeting signals enables PMP70 to correctly target to peroxisomal membranes...