Gene Symbol: Stat5b
Description: signal transducer and activator of transcription 5B
Alias: signal transducer and activator of transcription 5B, Signal transducer and activator of transcription 5a
Species: rat
Products:     Stat5b

Top Publications

  1. Ripperger J, Fritz S, Richter K, Hocke G, Lottspeich F, Fey G. Transcription factors Stat3 and Stat5b are present in rat liver nuclei late in an acute phase response and bind interleukin-6 response elements. J Biol Chem. 1995;270:29998-30006 pubmed
    ..the 86- and 91-kDa species as two forms of the transcription factor Stat3 and the 92-kDa species as the factor Stat5b. This identification was confirmed by gel mobility shift-supershift experiments using specific antisera for Stat3 ..
  2. Chia D, Ono M, Woelfle J, Schlesinger Massart M, Jiang H, Rotwein P. Characterization of distinct Stat5b binding sites that mediate growth hormone-stimulated IGF-I gene transcription. J Biol Chem. 2006;281:3190-7 pubmed mechanisms independent of new protein synthesis, and recent studies have linked the transcription factor Stat5b to a regulatory network connecting the activated GH receptor on the cell membrane to the IGF-I gene in the nucleus...
  3. Moriggl R, Sexl V, Piekorz R, Topham D, Ihle J. Stat5 activation is uniquely associated with cytokine signaling in peripheral T cells. Immunity. 1999;11:225-30 pubmed
    ..Mice deficient in Stat5a and Stat5b have demonstrated the essential role that these highly related proteins play in cell cycle progression following ..
  4. Woelfle J, Rotwein P. In vivo regulation of growth hormone-stimulated gene transcription by STAT5b. Am J Physiol Endocrinol Metab. 2004;286:E393-401 pubmed
    ..Recent studies in mice have implicated the transcription factor STAT5b as part of the GH-regulated somatic growth pathway, because mice lacking this protein showed diminished growth ..
  5. Woelfle J, Chia D, Rotwein P. Mechanisms of growth hormone (GH) action. Identification of conserved Stat5 binding sites that mediate GH-induced insulin-like growth factor-I gene activation. J Biol Chem. 2003;278:51261-6 pubmed
    ..Recent studies implicating Stat5a and Stat5b in the growth response to GH in mice and observations linking Stat5b to control of IGF-I gene transcription in ..
  6. Chia D, Varco Merth B, Rotwein P. Dispersed Chromosomal Stat5b-binding elements mediate growth hormone-activated insulin-like growth factor-I gene transcription. J Biol Chem. 2010;285:17636-47 pubmed publisher
    ..Recently described inactivating mutations in Stat5b in humans with impaired growth have focused attention on this transcription factor as a key agent linking GH-..
  7. Sexl V, Piekorz R, Moriggl R, Rohrer J, Brown M, Bunting K, et al. Stat5a/b contribute to interleukin 7-induced B-cell precursor expansion, but abl- and bcr/abl-induced transformation are independent of stat5. Blood. 2000;96:2277-83 pubmed
    ..Using mice deficient for the 2 highly related transcription factors, Stat5a and Stat5b (Stat5a/b(-/-)), we investigated the role of Stat5 for B-cell differentiation, expansion, and function...
  8. Gerland K, Bataille Simoneau N, Basle M, Fourcin M, Gascan H, Mercier L. Activation of the Jak/Stat signal transduction pathway in GH-treated rat osteoblast-like cells in culture. Mol Cell Endocrinol. 2000;168:1-9 pubmed
    ..Altogether the results suggest that UMR-106.01 cells exhibit original features of the GH-dependent Jak/Stat signaling pathway. ..
  9. Woelfle J, Billiard J, Rotwein P. Acute control of insulin-like growth factor-I gene transcription by growth hormone through Stat5b. J Biol Chem. 2003;278:22696-702 pubmed
    ..Recent studies in mice have implicated Stat5b as part of a GH-regulated somatic growth pathway, because mice lacking this transcription factor show diminished ..

Scientific Experts

More Information


  1. Teglund S, McKay C, Schuetz E, van Deursen J, Stravopodis D, Wang D, et al. Stat5a and Stat5b proteins have essential and nonessential, or redundant, roles in cytokine responses. Cell. 1998;93:841-50 pubmed
    ..Among the Stats, the two highly related proteins, Stat5a and Stat5b, are activated by a variety of cytokines...
  2. Wu S, Morrison A, Sun H, De Luca F. Nuclear factor-kappaB (NF-kappaB) p65 interacts with Stat5b in growth plate chondrocytes and mediates the effects of growth hormone on chondrogenesis and on the expression of insulin-like growth factor-1 and bone morphogenetic protein-2. J Biol Chem. 2011;286:24726-34 pubmed publisher
    ..It has been shown that the transcription factor Stat5b mediates the GH promoting effect on IGF-1 expression and on chondrogenesis, yet it is not known whether other ..
  3. Park S, Waxman D. Inhibitory cross-talk between STAT5b and liver nuclear factor HNF3beta: impact on the regulation of growth hormone pulse-stimulated, male-specific liver cytochrome P-450 gene expression. J Biol Chem. 2001;276:43031-9 pubmed
    b>STAT5b is repeatedly activated in rodent liver by the male pattern of intermittent plasma growth hormone (GH) stimulation and is required to maintain the GH pulse-regulated, male-specific pattern of liver gene expression...
  4. Kang J, DiBenedetto B, Narayan K, Zhao H, Der S, Chambers C. STAT5 is required for thymopoiesis in a development stage-specific manner. J Immunol. 2004;173:2307-14 pubmed
  5. Anderson G, Beijer P, Bang A, Fenwick M, Bunn S, Grattan D. Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lact. Endocrinology. 2006;147:4996-5005 pubmed
    ..activation of dopamine synthesis in these neurons requires the Janus kinase/signal transducer and activator of transcription 5b (STAT5b) signaling pathway, we investigated whether prolactin-induced STAT5b signaling is ..
  6. Vidal O, Merino R, Rico Bautista E, Fernandez Perez L, Chia D, Woelfle J, et al. In vivo transcript profiling and phylogenetic analysis identifies suppressor of cytokine signaling 2 as a direct signal transducer and activator of transcription 5b target in liver. Mol Endocrinol. 2007;21:293-311 pubmed
    The GH-activated signal transducer and activator of transcription 5b (STAT5b) is an essential regulator of somatic growth. The transcriptional response to STAT5b in liver is poorly understood...
  7. Mihailovic M, Dinić S, Bogojevic D, Ivanovic Matic S, Uskoković A, Arambasić J, et al. Nuclear localization and binding affinity of STAT5b for the alpha(2)-macroglobulin gene promoter during rat liver development and the acute-phase response. Acta Biochim Pol. 2007;54:331-40 pubmed
    ..Here we examined the involvement of STAT5b in MG gene expression during development and the AP response...
  8. Venge P, Zetterstrom O, Dahl R, Roxin L, Olsson I. Low levels of eosinophil cationic proteins in patients with asthma. Lancet. 1977;2:373-5 pubmed
    ..Assays of serum "eosinophil" cationic proteins may be useful in the differentiation of atopic and intrinsic asthma and may throw light on pathogenesis. ..
  9. Murray P. The JAK-STAT signaling pathway: input and output integration. J Immunol. 2007;178:2623-9 pubmed
    ..New data suggests that SOCS proteins introduce additional diversity into the JAK-STAT pathway by adjusting the output of activated STATs that alters downstream gene activation. ..
  10. Rhodes J, York R, Tara D, Tajinda K, Druker B. CrkL functions as a nuclear adaptor and transcriptional activator in Bcr-Abl-expressing cells. Exp Hematol. 2000;28:305-10 pubmed
    ..This suggests a novel role for CrkL, functioning as a nuclear adaptor protein that can associate with and activate Stat proteins in Bcr-Abl-expressing cells. ..
  11. Fernandez D, Tuma D, Tuma P. Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3. Am J Physiol Gastrointest Liver Physiol. 2012;303:G1402-15 pubmed publisher
    ..To represent factors that undergo directed delivery, we examined growth hormone-induced STAT5B translocation and IL-6-induced STAT3 translocation...
  12. Chen Y, Sun D, Krishnamurthy V, Rabkin R. Endotoxin attenuates growth hormone-induced hepatic insulin-like growth factor I expression by inhibiting JAK2/STAT5 signal transduction and STAT5b DNA binding. Am J Physiol Endocrinol Metab. 2007;292:E1856-62 pubmed
    ..Nuclear phosphorylated STAT5b levels were significantly depressed to 61% of the control values and represent a potential cause of the reduced GH-..
  13. Grigorov I, Lazic T, Cvetkovic I, Milosavljevic T, Petrovic M. Opposite nuclear level and binding activity of STAT5B and STAT3 proteins with rat haptoglobin gene under normal and turpentine induced acute phase conditions. Mol Biol Rep. 2001;28:217-22 pubmed
    ..that under normal but not in the turpentine induced AP conditions, another member of the STAT family proteins, STAT5b is expressed and binds to the hormone regulatory element (HRE) of the rat Hp gene...
  14. Vignudelli T, Selmi T, Martello A, Parenti S, Grande A, Gemelli C, et al. ZFP36L1 negatively regulates erythroid differentiation of CD34+ hematopoietic stem cells by interfering with the Stat5b pathway. Mol Biol Cell. 2010;21:3340-51 pubmed publisher
    ..that ZFP36L1 negatively regulates erythroid differentiation by directly binding the 3' untranslated region of Stat5b encoding mRNA...
  15. Dhennin Duthille I, Nyga R, Yahiaoui S, Gouilleux Gruart V, Regnier A, Lassoued K, et al. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J Biol Chem. 2011;286:5034-42 pubmed publisher
    ..Because STAT5a and STAT5b differ mainly in the carboxyl-terminal transactivation domain, we sought to identify new proteins that bind ..
  16. Kazansky A, Spencer D, Greenberg N. Activation of signal transducer and activator of transcription 5 is required for progression of autochthonous prostate cancer: evidence from the transgenic adenocarcinoma of the mouse prostate system. Cancer Res. 2003;63:8757-62 pubmed
    ..The STAT family has at least seven members, and the two forms of the STAT5 protein, STAT5A and STAT5B, exhibit a high degree of sequence similarity...
  17. Fernando J, Faber T, Pullen N, Falanga Y, Kolawole E, Oskeritzian C, et al. Genotype-dependent effects of TGF-?1 on mast cell function: targeting the Stat5 pathway. J Immunol. 2013;191:4505-13 pubmed publisher
    ..These changes may partly explain the effects of TGF-?1, because Stat5B overexpression blocked TGF-mediated suppression of IgE-induced cytokine production...
  18. Jackerott M, Møldrup A, Thams P, Galsgaard E, Knudsen J, Lee Y, et al. STAT5 activity in pancreatic beta-cells influences the severity of diabetes in animal models of type 1 and 2 diabetes. Diabetes. 2006;55:2705-12 pubmed
    ..transgenic mice that expressed a dominant-negative mutant of STAT5a (DNSTAT5) or constitutive active mutant of STAT5b (CASTAT5) under control of the rat insulin 1 promoter (RIP)...
  19. Sun S, Li T, Yang P, Qiu Y, Rui Y. Modulation of signal transducers and activators of transcription (STAT) factor pathways during focal cerebral ischaemia: a gene expression array study in rat hippocampus after middle cerebral artery occlusion. Clin Exp Pharmacol Physiol. 2007;34:1097-101 pubmed
  20. Yi W, Kim S, Jiang J, Park S, Kraft A, Waxman D, et al. Growth hormone receptor cytoplasmic domain differentially promotes tyrosine phosphorylation of signal transducers and activators of transcription 5b and 3 by activated JAK2 kinase. Mol Endocrinol. 1996;10:1425-43 pubmed
    ..The present study investigates the importance of the GHR cytoplasmic domain in the activation of STAT3 and STAT5b. As the perimembranous Box1 region of the GHR cytoplasmic domain is necessary for activation of wild-type (WT) ..
  21. Gebert C, Park S, Waxman D. Regulation of signal transducer and activator of transcription (STAT) 5b activation by the temporal pattern of growth hormone stimulation. Mol Endocrinol. 1997;11:400-14 pubmed
    ..followed by serine or threonine phosphorylation, leading to activation of the DNA-binding activity of STAT5b, the major STAT5 form present in these cells...
  22. Guren T, Ødegård J, Abrahamsen H, Thoresen G, Susa M, Andersson Y, et al. EGF receptor-mediated, c-Src-dependent, activation of Stat5b is downregulated in mitogenically responsive hepatocytes. J Cell Physiol. 2003;196:113-23 pubmed
    ..We previously showed that Stat5b was activated by EGF in rat hepatocytes in primary monolayer culture...
  23. Wiwi C, Waxman D. Role of hepatocyte nuclear factors in growth hormone-regulated, sexually dimorphic expression of liver cytochromes P450. Growth Factors. 2004;22:79-88 pubmed
    ..These HNFs are proposed to collaborate with the GH pulse-activated latent cytoplasmic transcription factor STAT5b to regulate the sex-dependent expression of liver CYPs...
  24. Laz E, Holloway M, Chen C, Waxman D. Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver. Endocrinology. 2007;148:3327-37 pubmed
    ..of two transcription factors implicated in GH regulation of liver sex specificity, namely, signal transducer and activator of transcription 5b (STAT5b) and hepatocyte nuclear factor 4alpha (HNF4alpha), with sex-specific expression ..
  25. Cao H, Dronadula N, Rizvi F, Li Q, Srivastava K, Gerthoffer W, et al. Novel role for STAT-5B in the regulation of Hsp27-FGF-2 axis facilitating thrombin-induced vascular smooth muscle cell growth and motility. Circ Res. 2006;98:913-22 pubmed
  26. Yamazaki T, Kanzaki M, Kamidono S, Fujisawa M. Effect of erythropoietin on Leydig cell is associated with the activation of Stat5 pathway. Mol Cell Endocrinol. 2004;213:193-8 pubmed
    ..Expression of Stat5b protein and phosphorylated Stat5b protein were investigated by Western blotting using anti-Stat5b pAb and anti-..
  27. Zhang S, Wei J, Li C, Barnstable C, Fu X. Expression and activation of STAT proteins during mouse retina development. Exp Eye Res. 2003;76:421-31 pubmed
    ..Studies of STAT signal pathways in the eye may contribute to the understanding of molecular mechanisms in control of ocular development and pathogenesis. ..
  28. Joung Y, Lim E, Darvin P, Chung S, Jang J, Do Park K, et al. MSM enhances GH signaling via the Jak2/STAT5b pathway in osteoblast-like cells and osteoblast differentiation through the activation of STAT5b in MSCs. PLoS ONE. 2012;7:e47477 pubmed publisher
    ..MSM increased the expression of GH-related proteins including IGF-1R, p-IGF-1R, STAT5b, p-STAT5b, and Jak2 in osteoblastic cells and MSCs...
  29. Krebs C, Schultz D, Robins D. The KRAB zinc finger protein RSL1 regulates sex- and tissue-specific promoter methylation and dynamic hormone-responsive chromatin configuration. Mol Cell Biol. 2012;32:3732-42 pubmed publisher
    ..2 kb upstream of the Slp promoter, both in vitro and in vivo, within an enhancer containing response elements for STAT5b. Chromatin immunoprecipitation (ChIP) assays demonstrate that RSL1 recruits KAP1/TRIM28, the corepressor for KRAB ..
  30. Kieslinger M, Woldman I, Moriggl R, Hofmann J, Marine J, Ihle J, et al. Antiapoptotic activity of Stat5 required during terminal stages of myeloid differentiation. Genes Dev. 2000;14:232-44 pubmed
    ..findings with dnStat5-expressing chicken myeloblasts were confirmed with primary hematopoietic cells from Stat5a/Stat5b-deficient mice...
  31. Russell D, Richards J. Differentiation-dependent prolactin responsiveness and stat (signal transducers and activators of transcription) signaling in rat ovarian cells. Mol Endocrinol. 1999;13:2049-64 pubmed
    ..Rather PRL activation of Stat5, principally Stat5b, occurred in association with luteinization...
  32. Leung K, Doyle N, Ballesteros M, Sjogren K, Watts C, Low T, et al. Estrogen inhibits GH signaling by suppressing GH-induced JAK2 phosphorylation, an effect mediated by SOCS-2. Proc Natl Acad Sci U S A. 2003;100:1016-21 pubmed
    ..In conclusion, estrogen inhibits GH signaling, an action mediated by SOCS-2. This paper provides evidence for regulatory interaction between a sex steroid and the GHJAKSTAT pathway, in which SOCS-2 plays a central mechanistic role. ..
  33. Piekorz R, Gingras S, Hoffmeyer A, Ihle J, Weinstein Y. Regulation of progesterone levels during pregnancy and parturition by signal transducer and activator of transcription 5 and 20alpha-hydroxysteroid dehydrogenase. Mol Endocrinol. 2005;19:431-40 pubmed
    The two highly related signal transducers and activators of transcription (Stats), Stat5a and Stat5b, are major mediators of prolactin signaling in both the mammary gland and in the ovary...
  34. Imada K, Bloom E, Nakajima H, Horvath Arcidiacono J, Udy G, Davey H, et al. Stat5b is essential for natural killer cell-mediated proliferation and cytolytic activity. J Exp Med. 1998;188:2067-74 pubmed
    ..that can be overcome by high dose interleukin (IL)-2, we now demonstrate that defective proliferation in Stat5b-/- splenocytes cannot be corrected by this treatment...
  35. Brelje T, Stout L, Bhagroo N, Sorenson R. Distinctive roles for prolactin and growth hormone in the activation of signal transducer and activator of transcription 5 in pancreatic islets of langerhans. Endocrinology. 2004;145:4162-75 pubmed
    ..The activation of STAT5B by PRL was biphasic, with an initial peak within 30 min, a nadir between 1 and 3 h, and prolonged activation after ..
  36. Badger T, Ronis M, Frank S, Chen Y, He L. Effects of chronic ethanol on hepatic and renal CYP2C11 in the male rat: interactions with the Janus-kinase 2-signal transducer and activators of transcription proteins 5b pathway. Endocrinology. 2003;144:3969-76 pubmed
    ..Renal CYP2C11 is regulated by testosterone, not GH. The involvement of the JAK/STAT5b signal transduction pathway in renal CYP2C11 signaling has not been studied...
  37. Varco Merth B, Mirza K, Alzhanov D, Chia D, Rotwein P. Biochemical characterization of diverse Stat5b-binding enhancers that mediate growth hormone-activated insulin-like growth factor-I gene transcription. PLoS ONE. 2012;7:e50278 pubmed publisher
    ..growth factor I (IGF-I), a 70-amino acid secreted peptide whose gene expression is rapidly induced by GH via the Stat5b transcription factor...
  38. Yiu G, Hecht N. Novel testis-specific protein-DNA interactions activate transcription of the mouse protamine 2 gene during spermatogenesis. J Biol Chem. 1997;272:26926-33 pubmed
    ..These data suggest that the testis-specific PAF-1 and a Y-box-binding protein are needed to activate mP2 transcription in postmeiotic male germ cells. ..
  39. Schirra H, Anderson C, Wilson W, Kerr L, Craik D, Waters M, et al. Altered metabolism of growth hormone receptor mutant mice: a combined NMR metabonomics and microarray study. PLoS ONE. 2008;3:e2764 pubmed publisher
  40. Chia D, Subbian E, Buck T, Hwa V, Rosenfeld R, Skach W, et al. Aberrant folding of a mutant Stat5b causes growth hormone insensitivity and proteasomal dysfunction. J Biol Chem. 2006;281:6552-8 pubmed
    A predicted alanine to proline substitution in Stat5b that results in profound short stature, growth hormone insensitivity, and immunodeficiency represents the first natural mutation of this transcription factor in a human...
  41. Peng S, Lai Y, Huang H, Huang H, Huang Y. A novel role of CPEB3 in regulating EGFR gene transcription via association with Stat5b in neurons. Nucleic Acids Res. 2010;38:7446-57 pubmed publisher
    ..Here, we have identified a novel function of CPEB3 as to interact with Stat5b and inhibit its transcription activity in the nucleus without disrupting dimerization, DNA binding and nuclear ..
  42. Ji S, Guan R, Frank S, Messina J. Insulin inhibits growth hormone signaling via the growth hormone receptor/JAK2/STAT5B pathway. J Biol Chem. 1999;274:13434-42 pubmed
    ..insulin treatment of hepatoma cells results in a time-dependent inhibition of acute GH-induced phosphorylation of STAT5B. Whereas total protein levels of JAK2 were not reduced after insulin pretreatment for 16 h, GH-induced JAK2 ..
  43. Miyoshi K, Shillingford J, Smith G, Grimm S, Wagner K, Oka T, et al. Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium. J Cell Biol. 2001;155:531-42 pubmed
    ..These data demonstrate that signaling via the PrlR and Stat5 is critical for the proliferation and differentiation of mammary alveoli during pregnancy. ..
  44. Youngren J. Regulation of insulin receptor function. Cell Mol Life Sci. 2007;64:873-91 pubmed
    ..The impact of these processes on the conformational changes and phosphorylation events required for full signaling activity, as well as the role of these mechanisms in human disease, is reviewed in this article. ..
  45. Thangavel C, Shapiro B. A molecular basis for the sexually dimorphic response to growth hormone. Endocrinology. 2007;148:2894-903 pubmed
    ..Dynamic changes in the Janus kinase-2 (Jak2)/signal transducers and activators of transcription (Stat5B) signaling pathway [as determined by transducer activation, Stat5B binding to the GH-responsive promoter of the ..
  46. Olsen H, Hedengran Faulds M, Saharinen P, Silvennoinen O, Haldosén L. Effects of hyperactive Janus kinase 2 signaling in mammary epithelial cells. Biochem Biophys Res Commun. 2002;296:139-44 pubmed
    ..Proliferation was not affected by any of the mutants whereas the ability to undergo apoptosis was decreased implicating a transforming potential of the JAK2 mutants. ..
  47. Timsit Y, Riddick D. Stimulation of hepatic signal transducer and activator of transcription 5b by GH is not altered by 3-methylcholanthrene. Endocrinology. 2002;143:3284-94 pubmed
    ..In follow-up studies we evaluated the effect of MC on GH-stimulated signal transducer and activator of transcription 5b (STAT5b) phosphorylation, nuclear translocation, and DNA-binding activity...
  48. Volgin D, Kubin L. Chronic intermittent hypoxia alters hypothalamic transcription of genes involved in metabolic regulation. Auton Neurosci. 2006;126-127:93-9 pubmed
    ..mRNA levels of three members of the family of signal transducers and activators of transcription, STAT1, STAT3 and STAT5b, were also increased 2-4 times...