Slc8a1

Summary

Gene Symbol: Slc8a1
Description: solute carrier family 8 member A1
Alias: Ncx, Ncx1, sodium/calcium exchanger 1, Na(+)/Ca(2+)-exchange protein 1, Na-Ca exchanger, solute carrier family 8 (sodium/calcium exchanger), member 1, solute carrier family 8 member 1
Species: rat
Products:     Slc8a1

Top Publications

  1. Kieval R, Bloch R, Lindenmayer G, Ambesi A, Lederer W. Immunofluorescence localization of the Na-Ca exchanger in heart cells. Am J Physiol. 1992;263:C545-50 pubmed
    ..Our finding that the Na-Ca exchanger is widely distributed over the cell surface may provide further insight into the role of Na-Ca exchange in the heart...
  2. Okumura R, Shibukawa Y, Muramatsu T, Hashimoto S, Nakagawa K, Tazaki M, et al. Sodium-calcium exchangers in rat ameloblasts. J Pharmacol Sci. 2010;112:223-30 pubmed
    ..RT-PCR and immunohistochemical analyses revealed expression of NCX1 and NCX3 in ameloblasts, localized in the apical membrane...
  3. Tsumura M, Sobhan U, Muramatsu T, Sato M, Ichikawa H, Sahara Y, et al. TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. Cell Calcium. 2012;52:124-36 pubmed publisher
    ..KB-R7943, an NCX inhibitor, yielded an increase in the decay time constant during TRPV1-mediated Ca(2+) entry...
  4. Scriven D, Dan P, Moore E. Distribution of proteins implicated in excitation-contraction coupling in rat ventricular myocytes. Biophys J. 2000;79:2682-91 pubmed
    ..We conclude that if either the slip mode conductance of the Na(+) channel or the reverse mode of the Na(+)/Ca(2+) exchanger are to contribute to the contractile force, the fuzzy space must extend outside of the dyad. ..
  5. Lencesova L, O Neill A, Resneck W, Bloch R, Blaustein M. Plasma membrane-cytoskeleton-endoplasmic reticulum complexes in neurons and astrocytes. J Biol Chem. 2004;279:2885-93 pubmed
    ..Na(+)/Ca(2+) exchanger type 1 (NCX1), a key PM Ca(2+) transporter, was immunoprecipitated from the detergent-soluble fraction...
  6. Minelli A, Castaldo P, Gobbi P, Salucci S, Magi S, Amoroso S. Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat. Cell Calcium. 2007;41:221-34 pubmed
    ..we investigated by immunocytochemistry the cellular and subcellular localization of the three known NCX isoforms, NCX1, NCX2 and NCX3, in adult rat neocortex and hippocampus...
  7. Pott C, Goldhaber J, Philipson K. Genetic manipulation of cardiac Na+/Ca2+ exchange expression. Biochem Biophys Res Commun. 2004;322:1336-40 pubmed
    The Na+/Ca2+ exchanger (NCX) is the primary Ca2+ extrusion mechanism in cardiomyocytes...
  8. Shimizu J, Yamashita D, Misawa H, Tohne K, Matsuoka S, Kim B, et al. Increased O2 consumption in excitation-contraction coupling in hypertrophied rat heart slices related to increased Na+ -Ca2+ exchange activity. J Physiol Sci. 2009;59:63-74 pubmed publisher
    ..However, the increase in NCX current was not due to the increase in NCX1 protein expression, but was attributable to attenuation of the intrinsic inactivation mechanisms...
  9. Li J, Kline C, Hund T, Anderson M, Mohler P. Ankyrin-B regulates Kir6.2 membrane expression and function in heart. J Biol Chem. 2010;285:28723-30 pubmed publisher
    ..Collectively, our new findings define a new role for cardiac ankyrin polypeptides in regulation of ion channel membrane expression in heart...
  10. Kokubo Y, Inamoto N, Tomoike H, Kamide K, Takiuchi S, Kawano Y, et al. Association of genetic polymorphisms of sodium-calcium exchanger 1 gene, NCX1, with hypertension in a Japanese general population. Hypertens Res. 2004;27:697-702 pubmed
    ..Therefore, we examined whether genetic variations in NCX1 were associated with hypertension...

Detail Information

Publications95

  1. Kieval R, Bloch R, Lindenmayer G, Ambesi A, Lederer W. Immunofluorescence localization of the Na-Ca exchanger in heart cells. Am J Physiol. 1992;263:C545-50 pubmed
    ..Our finding that the Na-Ca exchanger is widely distributed over the cell surface may provide further insight into the role of Na-Ca exchange in the heart...
  2. Okumura R, Shibukawa Y, Muramatsu T, Hashimoto S, Nakagawa K, Tazaki M, et al. Sodium-calcium exchangers in rat ameloblasts. J Pharmacol Sci. 2010;112:223-30 pubmed
    ..RT-PCR and immunohistochemical analyses revealed expression of NCX1 and NCX3 in ameloblasts, localized in the apical membrane...
  3. Tsumura M, Sobhan U, Muramatsu T, Sato M, Ichikawa H, Sahara Y, et al. TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. Cell Calcium. 2012;52:124-36 pubmed publisher
    ..KB-R7943, an NCX inhibitor, yielded an increase in the decay time constant during TRPV1-mediated Ca(2+) entry...
  4. Scriven D, Dan P, Moore E. Distribution of proteins implicated in excitation-contraction coupling in rat ventricular myocytes. Biophys J. 2000;79:2682-91 pubmed
    ..We conclude that if either the slip mode conductance of the Na(+) channel or the reverse mode of the Na(+)/Ca(2+) exchanger are to contribute to the contractile force, the fuzzy space must extend outside of the dyad. ..
  5. Lencesova L, O Neill A, Resneck W, Bloch R, Blaustein M. Plasma membrane-cytoskeleton-endoplasmic reticulum complexes in neurons and astrocytes. J Biol Chem. 2004;279:2885-93 pubmed
    ..Na(+)/Ca(2+) exchanger type 1 (NCX1), a key PM Ca(2+) transporter, was immunoprecipitated from the detergent-soluble fraction...
  6. Minelli A, Castaldo P, Gobbi P, Salucci S, Magi S, Amoroso S. Cellular and subcellular localization of Na+-Ca2+ exchanger protein isoforms, NCX1, NCX2, and NCX3 in cerebral cortex and hippocampus of adult rat. Cell Calcium. 2007;41:221-34 pubmed
    ..we investigated by immunocytochemistry the cellular and subcellular localization of the three known NCX isoforms, NCX1, NCX2 and NCX3, in adult rat neocortex and hippocampus...
  7. Pott C, Goldhaber J, Philipson K. Genetic manipulation of cardiac Na+/Ca2+ exchange expression. Biochem Biophys Res Commun. 2004;322:1336-40 pubmed
    The Na+/Ca2+ exchanger (NCX) is the primary Ca2+ extrusion mechanism in cardiomyocytes...
  8. Shimizu J, Yamashita D, Misawa H, Tohne K, Matsuoka S, Kim B, et al. Increased O2 consumption in excitation-contraction coupling in hypertrophied rat heart slices related to increased Na+ -Ca2+ exchange activity. J Physiol Sci. 2009;59:63-74 pubmed publisher
    ..However, the increase in NCX current was not due to the increase in NCX1 protein expression, but was attributable to attenuation of the intrinsic inactivation mechanisms...
  9. Li J, Kline C, Hund T, Anderson M, Mohler P. Ankyrin-B regulates Kir6.2 membrane expression and function in heart. J Biol Chem. 2010;285:28723-30 pubmed publisher
    ..Collectively, our new findings define a new role for cardiac ankyrin polypeptides in regulation of ion channel membrane expression in heart...
  10. Kokubo Y, Inamoto N, Tomoike H, Kamide K, Takiuchi S, Kawano Y, et al. Association of genetic polymorphisms of sodium-calcium exchanger 1 gene, NCX1, with hypertension in a Japanese general population. Hypertens Res. 2004;27:697-702 pubmed
    ..Therefore, we examined whether genetic variations in NCX1 were associated with hypertension...
  11. Shubair M, Oriowo M, Khan I. Expression of alternatively spliced variants of Na-Ca-exchanger-1 in experimental colitis: role in reduced colonic contractility. Mol Cell Biochem. 2012;370:15-21 pubmed publisher
    ..The main objective of this study was to identify the types of Na-Ca-exchanger-1 (NCX-1) variants expressed in the rat colon, and how this was affected by colitis...
  12. Lines G, Sande J, Louch W, Mørk H, Grøttum P, Sejersted O. Contribution of the Na+/Ca2+ exchanger to rapid Ca2+ release in cardiomyocytes. Biophys J. 2006;91:779-92 pubmed
    ..In this article, we investigate the contribution of the Na(+)/Ca(2+) exchanger (NCX) to the trigger Ca(2+)...
  13. Papa M, Canitano A, Boscia F, Castaldo P, Sellitti S, Porzig H, et al. Differential expression of the Na+-Ca2+ exchanger transcripts and proteins in rat brain regions. J Comp Neurol. 2003;461:31-48 pubmed
    ..and cellular distribution for both transcripts and proteins encoded by the three known Na(+)-Ca(2+) exchanger genes NCX1, NCX2, and NCX3...
  14. Schulze D, Pyrski M, Ruknudin A, Margolis J, Polumuri S, Margolis F. Sodium-calcium exchangers in olfactory tissue. Ann N Y Acad Sci. 2002;976:67-72 pubmed
  15. Miyamoto S, Howes A, Adams J, Dorn G, Brown J. Ca2+ dysregulation induces mitochondrial depolarization and apoptosis: role of Na+/Ca2+ exchanger and AKT. J Biol Chem. 2005;280:38505-12 pubmed
    ..Indeed, the Na(+)/Ca(2+) exchanger (NCX), which removes Ca(2+) from the cell, was markedly down-regulated at the mRNA and protein levels...
  16. Ahlers B, Zhang X, Moorman J, Rothblum L, Carl L, Song J, et al. Identification of an endogenous inhibitor of the cardiac Na+/Ca2+ exchanger, phospholemman. J Biol Chem. 2005;280:19875-82 pubmed
    Rapid and precise control of Na(+)/Ca(2+) exchanger (NCX1) activity is essential in the maintenance of beat-to-beat Ca(2+) homeostasis in cardiac myocytes...
  17. Wang J, Zhang X, Ahlers B, Carl L, Song J, Rothblum L, et al. Cytoplasmic tail of phospholemman interacts with the intracellular loop of the cardiac Na+/Ca2+ exchanger. J Biol Chem. 2006;281:32004-14 pubmed
    ..PLM), a member of the FXYD family of small ion transport regulators, inhibits cardiac Na+/Ca2+ exchanger (NCX1)...
  18. Pulina M, Rizzuto R, Brini M, Carafoli E. Inhibitory interaction of the plasma membrane Na+/Ca2+ exchangers with the 14-3-3 proteins. J Biol Chem. 2006;281:19645-54 pubmed
    The three Na+/Ca2+ exchanger isoforms, NCX1, NCX2, and NCX3, contain a large cytoplasmic loop that is responsible for the regulation of activity...
  19. Bojarski C, Meloni B, Moore S, Majda B, Knuckey N. Na+/Ca2+ exchanger subtype (NCX1, NCX2, NCX3) protein expression in the rat hippocampus following 3 min and 8 min durations of global cerebral ischemia. Brain Res. 2008;1189:198-202 pubmed
    There is increasing evidence that the sodium-calcium exchanger (NCX) subtypes, NCX1, NCX2 and NCX3 play an important role in intracellular calcium homeostasis/dysregulation following cerebral ischemia...
  20. Jacobs B, Liu Y, Pulina M, Golovina V, Hamlyn J. Normal pregnancy: mechanisms underlying the paradox of a ouabain-resistant state with elevated endogenous ouabain, suppressed arterial sodium calcium exchange, and low blood pressure. Am J Physiol Heart Circ Physiol. 2012;302:H1317-29 pubmed publisher
    Endogenous cardiotonic steroids (CTS) raise blood pressure (BP) via vascular sodium calcium exchange (NCX1.3) and transient receptor-operated channels (TRPCs)...
  21. Hong S, Lee J, Seo H, Lee C, Yoo K, Kim S, et al. Na(+)-Ca(2+) exchanger targeting miR-132 prevents apoptosis of cardiomyocytes under hypoxic condition by suppressing Ca(2+) overload. Biochem Biophys Res Commun. 2015;460:931-7 pubmed publisher
    ..One of the key regulators of the intracellular Ca(2+) level during IR injury is Na(+)-Ca(2+) exchanger 1 (NCX1), whose down-regulation during IR injury conferred protection of heart...
  22. Tappia P, Dent M, Aroutiounova N, Babick A, Weiler H. Gender differences in the modulation of cardiac gene expression by dietary conjugated linoleic acid isomers. Can J Physiol Pharmacol. 2007;85:465-75 pubmed
    ..reticulum Ca2+-ATPase (SERCA) 2a, ryanodine receptor, phospholamban, calsequestrin, Na+-Ca2+-exchanger (NCX), and L-type Ca2+ channel in the left ventricle (LV) by RT-PCR...
  23. Dong H, Dunn J, Lytton J. Stoichiometry of the Cardiac Na+/Ca2+ exchanger NCX1.1 measured in transfected HEK cells. Biophys J. 2002;82:1943-52 pubmed
    The stoichiometry with which the Na+/Ca2+ exchanger, NCX1, binds and transports Na+ and Ca2+ has dramatic consequences for ionic homeostasis and cellular function of heart mycocytes and brain neurons, where the exchanger is highly ..
  24. Dunn J, Elias C, Le H, Omelchenko A, Hryshko L, Lytton J. The molecular determinants of ionic regulatory differences between brain and kidney Na+/Ca2+ exchanger (NCX1) isoforms. J Biol Chem. 2002;277:33957-62 pubmed
    The Na(+)/Ca(2+) exchanger gene NCX1 undergoes alternative splicing leading to several isoforms that differ in a small portion of the large cytoplasmic loop...
  25. von Lewinski D, Stumme B, Fialka F, Luers C, Pieske B. Functional relevance of the stretch-dependent slow force response in failing human myocardium. Circ Res. 2004;94:1392-8 pubmed
    ..endothelin (ET) receptors (PD145065 or BQ123), Na+/H+ exchange (NHE1; HOE642), or reverse-mode Na+/Ca+ exchange (NCX; KB-R7493)...
  26. Sergeeva O, Amberger B, Eriksson K, Scherer A, Haas H. Co-ordinated expression of 5-HT2C receptors with the NCX1 Na+/Ca2+ exchanger in histaminergic neurones. J Neurochem. 2003;87:657-64 pubmed
    ..The majority of TM neurones express NCX1, NCX2 and NCKX3...
  27. Li X, Kiedrowski L, Tremblay F, Fernandez F, Perizzolo M, Winkfein R, et al. Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in motor learning and memory. J Biol Chem. 2006;281:6273-82 pubmed
    ..Neurons express several different Na+/Ca2+-exchangers belonging to both the K+-independent NCX family and the K+-dependent NCKX family...
  28. Sisalli M, Secondo A, Esposito A, Valsecchi V, Savoia C, Di Renzo G, et al. Endoplasmic reticulum refilling and mitochondrial calcium extrusion promoted in neurons by NCX1 and NCX3 in ischemic preconditioning are determinant for neuroprotection. Cell Death Differ. 2014;21:1142-9 pubmed publisher
    ..Considering the increased expression of the two sodium calcium exchanger (NCX) isoforms, NCX1 and NCX3, during cerebral ischemia and the relevance of nitric oxide (NO) in IPC modulation, we investigated ..
  29. Berra Romani R, Raqeeb A, Guzman Silva A, Torres Jácome J, Tanzi F, Moccia F. Na+-Ca2+ exchanger contributes to Ca2+ extrusion in ATP-stimulated endothelium of intact rat aorta. Biochem Biophys Res Commun. 2010;395:126-30 pubmed publisher
    The role of Na(+)-Ca(2+) exchanger (NCX) in vascular endothelium is still matter of debate...
  30. Formisano L, Guida N, Valsecchi V, Pignataro G, Vinciguerra A, Pannaccione A, et al. NCX1 is a new rest target gene: role in cerebral ischemia. Neurobiol Dis. 2013;50:76-85 pubmed publisher
    The Na(+)-Ca(2+) exchanger 1 (NCX1), a bidirectional transporter that mediates the electrogenic exchange of one calcium ion for three sodium ions across the plasma membrane, is known to be involved in brain ischemia...
  31. Yatabe M, Yatabe J, Takano K, Murakami Y, Sakuta R, Abe S, et al. Effects of a high-sodium diet on renal tubule Ca2+ transporter and claudin expression in Wistar-Kyoto rats. BMC Nephrol. 2012;13:160 pubmed publisher
    ..3% or 8% NaCl diet for 8 weeks. Renal cortical expressions of Na+/Ca2+ exchanger 1 (NCX1), Ca2+ pump (PCMA1b), Ca2+ channel (TRPV5), calbindin-D28k, and claudins (CLDN-2, -7, -8, -16 and -19) were ..
  32. Boscia F, Gala R, Pannaccione A, Secondo A, Scorziello A, di Renzo G, et al. NCX1 expression and functional activity increase in microglia invading the infarct core. Stroke. 2009;40:3608-17 pubmed publisher
    The sodium-calcium exchanger NCX1 represents a key mediator for maintaining [Na(+)](i) and [Ca(2+)](i) in anoxic conditions...
  33. Wang S, Hu D, Xi Q, Su S, Bai J, Liu J, et al. The expression and implication of TRPV5, Calbindin-D28k and NCX1 in idiopathic hypercalciuria. J Huazhong Univ Sci Technolog Med Sci. 2008;28:580-3 pubmed publisher
    The expression of calcium epithelium TRPV5, alcium binding protein Calbindin-D28k and Na(+)/Ca(2+) exchanger NCX1 was detected in renal distal convoluted tubule, and their effects on urine calcium reabsorption and the possible pathogenic ..
  34. Pignataro G, Gala R, Cuomo O, Tortiglione A, Giaccio L, Castaldo P, et al. Two sodium/calcium exchanger gene products, NCX1 and NCX3, play a major role in the development of permanent focal cerebral ischemia. Stroke. 2004;35:2566-70 pubmed
    ..In the brain, there are 3 different genes coding for 3 different proteins: NCX1, NCX2, and NCX3...
  35. Soliman D, Hamming K, Matemisz L, Light P. Reactive oxygen species directly modify sodium-calcium exchanger activity in a splice variant-dependent manner. J Mol Cell Cardiol. 2009;47:595-602 pubmed publisher
    The sodium-calcium exchanger isoform 1 (NCX1) operating in calcium-efflux mode plays an important role in maintaining calcium homeostasis in the heart...
  36. Yeung H, Kravtsov G, Ng K, Wong T, Fung M. Chronic intermittent hypoxia alters Ca2+ handling in rat cardiomyocytes by augmented Na+/Ca2+ exchange and ryanodine receptor activities in ischemia-reperfusion. Am J Physiol Cell Physiol. 2007;292:C2046-56 pubmed
    ..activity of (45)Ca(2+) flux of SR-Ca(2+)-ATPase, ryanodine receptor (RyR) and sarcolemmal Na(+)/Ca(2+) exchange (NCX) in ventricular myocytes from the CIH and normoxic groups before and during I/R...
  37. Ankorina Stark I, Amstrup J, Novak I. Regulation of the Na+/Ca2+ exchanger in rat pancreatic ducts. J Membr Biol. 2002;186:43-53 pubmed
    ..RT-PCR analysis indicated Na+/Ca2+-exchanger isoforms NCX1.3 and NCX1.7 in acini and pancreas...
  38. Reuter H, Henderson S, Han T, Mottino G, Frank J, Ross R, et al. Cardiac excitation-contraction coupling in the absence of Na(+) - Ca2+ exchange. Cell Calcium. 2003;34:19-26 pubmed
    We investigate cardiac excitation-contraction coupling in the absence of sarcolemmal Na(+) - Ca(2+) exchange using NCX1 knock out mice...
  39. Secondo A, Esposito A, Sirabella R, Boscia F, Pannaccione A, Molinaro P, et al. Involvement of the Na+/Ca2+ exchanger isoform 1 (NCX1) in neuronal growth factor (NGF)-induced neuronal differentiation through Ca2+-dependent Akt phosphorylation. J Biol Chem. 2015;290:1319-31 pubmed publisher
    ..We investigated whether NCX1, NCX2, and NCX3 isoforms could play a relevant role in neuronal differentiation through the modulation of [Ca(2+)]i ..
  40. Zhang X, Wang J, Song J, Ji A, Chan T, Cheung J. Residues 248-252 and 300-304 of the cardiac Na+/Ca2+ exchanger are involved in its regulation by phospholemman. Am J Physiol Cell Physiol. 2011;301:C833-40 pubmed publisher
    Using split cardiac Na(+)/Ca(2+) exchangers (NCX1), we previously demonstrated that phospholemman (PLM) regulates NCX1 by interacting with the proximal linker domain (residues 218-358) of the intracellular loop of NCX1...
  41. Sirabella R, Secondo A, Pannaccione A, Scorziello A, Valsecchi V, Adornetto A, et al. Anoxia-induced NF-kappaB-dependent upregulation of NCX1 contributes to Ca2+ refilling into endoplasmic reticulum in cortical neurons. Stroke. 2009;40:922-9 pubmed publisher
    The 3 gene products of the Na(+)/Ca(2+) exchanger (NCX), viz, NCX1, NCX2, and NCX3, may play a pivotal role in the pathophysiology of brain ischemia...
  42. Lee S, Yu A, Lytton J. Tissue-specific expression of Na(+)-Ca2+ exchanger isoforms. J Biol Chem. 1994;269:14849-52 pubmed
    ..Therefore, we propose that the structural complexity of the single NCE gene allows it to respond independently to the unique demands of different environments. ..
  43. Mohler P, Davis J, Bennett V. Ankyrin-B coordinates the Na/K ATPase, Na/Ca exchanger, and InsP3 receptor in a cardiac T-tubule/SR microdomain. PLoS Biol. 2005;3:e423 pubmed
    ..Ankyrin-B also is not abundantly expressed in smooth muscle. We propose that the ankyrin-B-based complex is a specialized adaptation of cardiomyocytes with a role for cytosolic Ca2+ modulation. ..
  44. Matayoshi T, Kamide K, Takiuchi S, Yoshii M, Miwa Y, Takami Y, et al. The thiazide-sensitive Na(+)-Cl(-) cotransporter gene, C1784T, and adrenergic receptor-beta3 gene, T727C, may be gene polymorphisms susceptible to the antihypertensive effect of thiazide diuretics. Hypertens Res. 2004;27:821-33 pubmed
    ..Forty-eight SNPs in 17 genes (ADD1, GNB3, TSC [SLC12A3], MLR [NR3C2], NCX1 [SLC8A1], WNK1, WNK4, AGT, ACE, AT1 [AGTR1], CYP11B2, ADRB1, ADRB2, ADRB3, ADRA1A, ADRA1B, ADRA2A) were genotyped in the ..
  45. Swift F, Franzini Armstrong C, Øyehaug L, Enger U, Andersson K, Christensen G, et al. Extreme sarcoplasmic reticulum volume loss and compensatory T-tubule remodeling after Serca2 knockout. Proc Natl Acad Sci U S A. 2012;109:3997-4001 pubmed publisher
    ..T-tubule proliferation occurs without loss of the original ordered transverse orientation and thus constitutes the basis for compensation of the declining SR function without structural disarrangement...
  46. Komuro I, Wenninger K, Philipson K, Izumo S. Molecular cloning and characterization of the human cardiac Na+/Ca2+ exchanger cDNA. Proc Natl Acad Sci U S A. 1992;89:4769-73 pubmed
    ..The levels of mRNA encoding the exchanger were significantly lower in fetal hearts than in adult hearts but were unchanged in the myocardium from patients with end-stage heart failure. ..
  47. Brini M, Manni S, Carafoli E. A study of the activity of the plasma membrane Na/Ca exchanger in the cellular environment. Ann N Y Acad Sci. 2002;976:376-81 pubmed
    ..Exchanger isoform 1 (NCX1) has been used for experiments in which the effect of its overexpression has been evaluated on the Ca homeostasis ..
  48. Kip S, Strehler E. Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress. Ann N Y Acad Sci. 2007;1099:436-9 pubmed
    ..found that after 2-3 h exposure to 300 microM H2O2, all NCXs and PMCAs were significantly downregulated at the RNA (NCX) and protein (PMCA) level. Rapid internalization and aggregation of the PMCA was also observed...
  49. Valsecchi V, Pignataro G, Del Prete A, Sirabella R, Matrone C, Boscia F, et al. NCX1 is a novel target gene for hypoxia-inducible factor-1 in ischemic brain preconditioning. Stroke. 2011;42:754-63 pubmed publisher
    The sodium-calcium exchanger-1 (NCX1) represents a key mediator for maintaining [Na(+)](i) and [Ca(2+)](i) homeostasis...
  50. Dong H, Light P, French R, Lytton J. Electrophysiological characterization and ionic stoichiometry of the rat brain K(+)-dependent NA(+)/CA(2+) exchanger, NCKX2. J Biol Chem. 2001;276:25919-28 pubmed
  51. Pulina M, Zulian A, Baryshnikov S, Linde C, Karashima E, Hamlyn J, et al. Cross talk between plasma membrane Na(+)/Ca (2+) exchanger-1 and TRPC/Orai-containing channels: key players in arterial hypertension. Adv Exp Med Biol. 2013;961:365-74 pubmed publisher
    Arterial smooth muscle (ASM) Na(+)/Ca(2+) exchanger type 1 (NCX1) and TRPC/Orai-containing receptor/store-operated cation channels (ROC/SOC) are clustered with ?2 Na(+) pumps in plasma membrane microdomains adjacent to the underlying ..
  52. Mace L, Palmer B, Brown D, Jew K, Lynch J, Glunt J, et al. Influence of age and run training on cardiac Na+/Ca2+ exchange. J Appl Physiol (1985). 2003;95:1994-2003 pubmed
    ..exchange after caffeine-induced Ca2+ release from the sarcoplasmic reticulum or cardiac Na+/Ca2+ exchanger protein (NCX1) expression...
  53. Wanichawan P, Hafver T, Hodne K, Aronsen J, Lunde I, Dalhus B, et al. Molecular basis of calpain cleavage and inactivation of the sodium-calcium exchanger 1 in heart failure. J Biol Chem. 2014;289:33984-98 pubmed publisher
    Cardiac sodium (Na(+))-calcium (Ca(2+)) exchanger 1 (NCX1) is central to the maintenance of normal Ca(2+) homeostasis and contraction. Studies indicate that the Ca(2+)-activated protease calpain cleaves NCX1...
  54. Kortus S, Srinivasan C, Forostyak O, Zapotocky M, Ueta Y, Sykova E, et al. Sodium-calcium exchanger and R-type Ca(2+) channels mediate spontaneous [Ca(2+)]i oscillations in magnocellular neurones of the rat supraoptic nucleus. Cell Calcium. 2016;59:289-98 pubmed publisher
    ..report in depth analysis of the contribution of plasmalemmal ion channels (Ca(2+), Na(+)), Na(+)/Ca(2+) exchanger (NCX), intracellular Ca(2+) release channels (InsP3Rs and RyRs), Ca(2+) storage organelles, plasma membrane Ca(2+) pump ..
  55. Ousingsawat J, Wanitchakool P, Schreiber R, Wuelling M, Vortkamp A, Kunzelmann K. Anoctamin-6 controls bone mineralization by activating the calcium transporter NCX1. J Biol Chem. 2015;290:6270-80 pubmed publisher
    ..The Na(+)/Ca(2+) exchanger NCX1 was found to interact with Ano6 in a two-hybrid split-ubiquitin screen...
  56. Nakasaki Y, Iwamoto T, Hanada H, Imagawa T, Shigekawa M. Cloning of the rat aortic smooth muscle Na+/Ca2+ exchanger and tissue-specific expression of isoforms. J Biochem. 1993;114:528-34 pubmed
    ..Alignment of the nucleotide sequences of these isoforms and Southern blot analysis of rat genomic DNA suggested that each isoform is generated through alternative splicing of the primary transcript. ..
  57. Wu M, Kao L, Liao H, Pan C. Reverse mode Na+/Ca2+ exchangers trigger the release of Ca2+ from intracellular Ca2+ stores in cultured rat embryonic cortical neurons. Brain Res. 2008;1201:41-51 pubmed publisher
    ..Using RT-PCR, several exchanger isoforms, ncx1, ncx3 and nckx2-4 were found to be expressed in freshly isolated and cultured cortical neurons...
  58. Li X, Lytton J. A circularized sodium-calcium exchanger exon 2 transcript. J Biol Chem. 1999;274:8153-60 pubmed
    Previous reports of Na/Ca exchanger gene 1 (NCX1) expression have revealed a major RNA transcript of 7 kilobase pairs (kb), minor transcripts of approximately 13 and approximately 4 kb, and a relatively abundant 1.8-kb RNA band...
  59. Riedel M, Baczkó I, Searle G, Webster N, Fercho M, Jones L, et al. Metabolic regulation of sodium-calcium exchange by intracellular acyl CoAs. EMBO J. 2006;25:4605-14 pubmed
    The sodium-calcium exchanger (NCX) is a critical mediator of calcium homeostasis. In the heart, NCX1 predominantly operates in forward mode to extrude Ca(2+); however, reverse-mode NCX1 activity during ischemia/reperfusion (IR) ..
  60. Mohammadi E, Bigdeli M. Effects of preconditioning with normobaric hyperoxia on Na?/Ca²? exchanger in the rat brain. Neuroscience. 2013;237:277-84 pubmed publisher
    ..Preconditioning with HO decreased NDS and infarct volume, and increased the expression of NCX1, NCX2 and NCX3 in the penumbra, NCX2, NCX3 in the core and NCX1 and NCX3 in the subcortex...
  61. Thurneysen T, Nicoll D, Philipson K, Porzig H. Immunohistochemical detection of the sodium-calcium exchanger in rat hippocampus cultures using subtype-specific antibodies. Ann N Y Acad Sci. 2002;976:367-75 pubmed
    All of the known Na+/Ca2+ exchanger subtypes, NCX1-3, are expressed in the brain, albeit with marked regional differences...
  62. Huang W, Dai B, Wen Z, Millard R, Yu X, Luther K, et al. Molecular strategy to reduce in vivo collagen barrier promotes entry of NCX1 positive inducible pluripotent stem cells (iPSC(NCX¹?)) into ischemic (or injured) myocardium. PLoS ONE. 2013;8:e70023 pubmed publisher
    ..In vitro: A tri-cell patch (Tri-P) consisting of cardiac sodium-calcium exchanger-1 (NCX1) positive iPSC (iPSC(NCX1+)), endothelial cells (EC), and mouse embryonic fibroblasts (MEF) was created, co-..
  63. Scheller T, Kraev A, Skinner S, Carafoli E. Cloning of the multipartite promoter of the sodium-calcium exchanger gene NCX1 and characterization of its activity in vascular smooth muscle cells. J Biol Chem. 1998;273:7643-9 pubmed
    The sodium-calcium exchange activity is mediated by proteins encoded in a small gene family, of which the gene NCX1 is ubiquitously expressed in mammalian tissues...
  64. Kobayashi K, Uchida S, Mizutani S, Sasaki S, Marumo F. Intrarenal and cellular localization of CLC-K2 protein in the mouse kidney. J Am Soc Nephrol. 2001;12:1327-34 pubmed
    ..These aspects of CLC-K2 localization suggest that CLC-K2 is important in Cl(-) transport in the distal nephron segments. ..
  65. Zhang S, Dong H, Rubin L, Yuan J. Upregulation of Na+/Ca2+ exchanger contributes to the enhanced Ca2+ entry in pulmonary artery smooth muscle cells from patients with idiopathic pulmonary arterial hypertension. Am J Physiol Cell Physiol. 2007;292:C2297-305 pubmed
    ..Here we report that protein expression of NCX1, an NCX family member of Na(+)/Ca(2+) exchanger proteins is upregulated in PASMC from IPAH patients compared with ..
  66. Lu J, Tong X, Wang X. Altered gene expression of Na+/Ca2+ exchanger isoforms NCX1, NCX2 and NCX3 in chronic ischemic rat brain. Neurosci Lett. 2002;332:21-4 pubmed
    To investigate the effect of chronic global cerebral ischemia on gene expression of Na(+)/Ca(2+) exchanger isoforms NCX1, NCX2 and NCX3 in rat brain...
  67. Cha S, Shin S, Jung S, Kim Y, Park Y, Kwak J, et al. Evidence for Na+/Ca2+ exchanger 1 association with caveolin-1 and -2 in C6 glioma cells. IUBMB Life. 2004;56:621-7 pubmed
    The purpose of this study is to understand the interaction of Na + -Ca2+ exchanger (NCX1), that is one of the essential regulators of Ca2+ homeostasis, with caveolin (Cav)-1 and Cav-2 in Cav-3 null cell (rat C6 glioma cell)...
  68. Dostanic I, Schultz J, Lorenz J, Lingrel J. The alpha 1 isoform of Na,K-ATPase regulates cardiac contractility and functionally interacts and co-localizes with the Na/Ca exchanger in heart. J Biol Chem. 2004;279:54053-61 pubmed
    ..These results demonstrate that the alpha 1 isoform regulates cardiac contractility, and that both the alpha 1 and alpha 2 isoforms are functionally and physically coupled with the Na/Ca exchanger in heart. ..
  69. Nagano T, Kawasaki Y, Baba A, Takemura M, Matsuda T. Up-regulation of Na(+)-Ca2+ exchange activity by interferon-gamma in cultured rat microglia. J Neurochem. 2004;90:784-91 pubmed
    ..RT-PCR showed that all of the known NCX isoforms, NCX1-3, are expressed in cultured microglia...
  70. Furman I, Cook O, Kasir J, Low W, Rahamimoff H. The putative amino-terminal signal peptide of the cloned rat brain Na(+)-Ca2+ exchanger gene (RBE-1) is not mandatory for functional expression. J Biol Chem. 1995;270:19120-7 pubmed
    ..Our results suggest that the putative signal peptide of the cloned Na(+)-Ca2+ exchanger gene does not play a mandatory role in functional expression of the protein in HeLa cells. ..
  71. Jordan M, Quednau B, Roos K, Ross R, Philipson K, Nicholas S. Cyclosporin A regulates sodium-calcium exchanger (NCX1) gene expression in vitro and cardiac hypertrophy in NCX1 transgenic mice. Ann N Y Acad Sci. 2002;976:259-67 pubmed
    The cardiac-specific sodium-calcium exchanger (NCX1) is a GATA-4 dependent gene that is upregulated during cardiac hypertrophy and heart failure...
  72. Magi S, Lariccia V, Castaldo P, Arcangeli S, Nasti A, Giordano A, et al. Physical and functional interaction of NCX1 and EAAC1 transporters leading to glutamate-enhanced ATP production in brain mitochondria. PLoS ONE. 2012;7:e34015 pubmed publisher
    ..colocalization, mutual activity dependency, physical interaction between EAAC1 and the sodium/calcium exchanger 1 (NCX1) both in neuronal and glial mitochondria, and that NCX1 is an essential modulator of this glutamate transporter...
  73. Sánchez J, López Zapata D, Francis L, De Los Reyes L. Effects of estradiol and IGF-1 on the sodium calcium exchanger in rat cultured cortical neurons. Cell Mol Neurobiol. 2011;31:619-27 pubmed publisher
    The Na(+)/Ca(2+) exchanger (NCX) is an important bidirectional transporter of calcium in neurons and has been shown to be involved in neuroprotection...
  74. Kitao T, Takuma K, Kawasaki T, Inoue Y, Ikehara A, Nashida T, et al. The Na+/Ca2+ exchanger-mediated Ca2+ influx triggers nitric oxide-induced cytotoxicity in cultured astrocytes. Neurochem Int. 2010;57:58-66 pubmed publisher
    ..In addition, SNP-induced (45)Ca(2+) influx and cytotoxicity were reduced in NCX1-deficient cells which were transfected with NCX1 siRNA...
  75. Boyman L, Hiller R, Lederer W, Khananshvili D. Direct Loading of the purified endogenous inhibitor into the cytoplasm of patched cardiomyocytes blocks the ion currents and calcium transport through the NCX1 protein. Biochemistry. 2008;47:6602-11 pubmed publisher
    The Na(+)-Ca(2+) exchanger in mammalian heart muscle (NCX1) is the central transporter protein that regulates extrusion of Ca(2+) from the heart cell...
  76. Hwang E, Williams I, Kovacs G, Peti Peterdi J, Siroky B, Rice W, et al. Impaired ability of the Na+/Ca2+ exchanger from the Dahl/Rapp salt-sensitive rat to regulate cytosolic calcium. Am J Physiol Renal Physiol. 2003;284:F1023-31 pubmed
    We previously cloned Na(+)/Ca(2+) exchanger (NCX1) from mesangial cells of salt-sensitive (SNCX = NCX1.7) and salt-resistant (RNCX = NCX1.3) Dahl/Rapp rats...
  77. Mohammadi E, Bigdeli M. Time course of neuroprotection induced by normobaric hyperoxia and NCX1 expression. Brain Inj. 2014;28:1127-34 pubmed publisher
    ..hyperoxia (HO) pre-conditioning offers durable neuroprotection against cerebral ischaemia and its effects on NCX1 expression. Rats were divided into two experimental groups...
  78. Unlap M, Peti Peterdi J, Bell P. Cloning of mesangial cell Na(+)/Ca(2+) exchangers from Dahl/Rapp salt-sensitive/resistant rats. Am J Physiol Renal Physiol. 2000;279:F177-84 pubmed
    ..the S rat. Lack of PKC activation of SNCX1 may contribute to a dysregulation of intracellular Ca(2+) concentration and enhanced renal vasoreactivity in this model of hypertension...
  79. Ketelaars S, Gorter J, Aronica E, Wadman W. Calcium extrusion protein expression in the hippocampal formation of chronic epileptic rats after kainate-induced status epilepticus. Epilepsia. 2004;45:1189-201 pubmed
    ..Hippocampal-parahippocampal protein expression of NCX1, 2, and 3, PMCA1-4, and NCKX2 at an early and late stage after kainate-induced status epilepticus (SE) was compared ..
  80. Hurtado C, Ander B, Maddaford T, Lukas A, Hryshko L, Pierce G. Adenovirally delivered shRNA strongly inhibits Na+-Ca2+ exchanger expression but does not prevent contraction of neonatal cardiomyocytes. J Mol Cell Cardiol. 2005;38:647-54 pubmed
    The cardiac Na(+)-Ca(2+) exchanger (NCX1) is the main mechanism for Ca(2+) efflux in the heart and is thought to serve an essential role in cardiac excitation-contraction (E-C) coupling...
  81. Lytton J. Na+/Ca2+ exchangers: three mammalian gene families control Ca2+ transport. Biochem J. 2007;406:365-82 pubmed
    ..The NCX (Na+/Ca2+ exchanger) [SLC (solute carrier) 8] branch of Na+/Ca2+ exchangers comprises three members: NCX1 has been most extensively studied, and is broadly expressed with particular abundance in heart, brain and kidney, ..
  82. Boscia F, Casamassa A, Secondo A, Esposito A, Pannaccione A, Sirabella R, et al. NCX1 Exchanger Cooperates with Calretinin to Confer Preconditioning-Induced Tolerance Against Cerebral Ischemia in the Striatum. Mol Neurobiol. 2016;53:1365-76 pubmed publisher
    Recently, the Na(+)/Ca(+2) exchanger NCX1 and the calcium binding protein calretinin have emerged as new molecular effectors of delayed preconditioning in the brain...
  83. Dong H, Jiang Y, Triggle C, Li X, Lytton J. Novel role for K+-dependent Na+/Ca2+ exchangers in regulation of cytoplasmic free Ca2+ and contractility in arterial smooth muscle. Am J Physiol Heart Circ Physiol. 2006;291:H1226-35 pubmed
    ..Na+/Ca2+ exchange (NCX) activity in the regulation of vascular Ca2+ homeostasis was previously ascribed to the NCX1 protein...
  84. Wansleeben C, Feitsma H, Tertoolen L, Kroon C, Guryev V, Cuppen E, et al. A novel mutant allele of Ncx1: a single amino acid substitution leads to cardiac dysfunction. Int J Dev Biol. 2010;54:1465-71 pubmed publisher
    The biological role and structure-function relationship of the Na(+)Ca(2+) exchanger NCX1 have been the subject of much investigation...
  85. Brustovetsky T, Pellman J, Yang X, Khanna R, Brustovetsky N. Collapsin response mediator protein 2 (CRMP2) interacts with N-methyl-D-aspartate (NMDA) receptor and Na+/Ca2+ exchanger and regulates their functional activity. J Biol Chem. 2014;289:7470-82 pubmed publisher
    ..Co-immunoprecipitation experiments revealed an interaction between CRMP2 and NMDAR as well as NCX3 but not NCX1. TAT-CBD3 disrupted CRMP2-NMDAR interaction without change in NMDAR localization...
  86. Paluzzi S, Alloisio S, Zappettini S, Milanese M, Raiteri L, Nobile M, et al. Adult astroglia is competent for Na+/Ca2+ exchanger-operated exocytotic glutamate release triggered by mild depolarization. J Neurochem. 2007;103:1196-207 pubmed
    ..To conclude, depolarization triggers in vitro glutamate exocytosis from in situ matured adult astrocytes; an aptitude grounding on Ca2+ influx driven by the Na+/Ca2+ exchanger working in the reverse mode. ..
  87. Wu F, Wei G, Li W, Liu B, Zhou J, Wang H, et al. Low extracellular K+ increases intracellular Ca2+ oscillation and injury by activating the reverse mode Na+-Ca2+ exchanger and inhibiting the Na+, K+ ATPase in rat cardiomyocytes. Int J Cardiol. 2010;140:161-8 pubmed publisher
    ..Measurements of cell length, reverse-mode Na(+)-Ca(2+) exchanger (NCX) and Na(+), K(+) ATPase activity were performed...