Gene Symbol: Slc6a5
Description: solute carrier family 6 member 5
Alias: GLYT2, sodium- and chloride-dependent glycine transporter 2, glyT-2, glycine transporter 2, solute carrier family 6 (neurotransmitter transporter), member 5, solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Species: rat
Products:     Slc6a5

Top Publications

  1. Pérez Siles G, Núñez E, Morreale A, Jiménez E, Leo Macias A, Pita G, et al. An aspartate residue in the external vestibule of GLYT2 (glycine transporter 2) controls cation access and transport coupling. Biochem J. 2012;442:323-34 pubmed publisher
    ..In contrast, neuronal GLYT2 supplies glycine to the presynaptic terminal with a 3:1:1 stoichiometry...
  2. de Juan Sanz J, Zafra F, López Corcuera B, Aragón C. Endocytosis of the neuronal glycine transporter GLYT2: role of membrane rafts and protein kinase C-dependent ubiquitination. Traffic. 2011;12:1850-67 pubmed publisher
    ..The neuronal glycine transporter 2 (GLYT2) supplies the terminal with substrate to refill synaptic vesicles containing glycine...
  3. Núñez E, Alonso Torres P, Fornés A, Aragón C, López Corcuera B. The neuronal glycine transporter GLYT2 associates with membrane rafts: functional modulation by lipid environment. J Neurochem. 2008;105:2080-90 pubmed publisher
    ..Missense mutations in the gene encoding GLYT2 (SLC6A5) cause hyperekplexia in humans. The activity of GLYT2 seems to be highly regulated...
  4. Nakamura M, Jang I. GlyT-2 mediates the forskolin-induced increase of glycinergic transmission. Neuroreport. 2010;21:463-8 pubmed publisher
  5. Geerlings A, Lopez Corcuera B, Aragon C. Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A. FEBS Lett. 2000;470:51-4 pubmed
    In this study we have examined the effect of the SNARE protein syntaxin 1A on the glycine transporters GLYT1 and GLYT2. Our results demonstrate a functional and physical interaction between both glycine transporters and syntaxin 1A...
  6. de Juan Sanz J, Núñez E, Zafra F, Berrocal M, Corbacho I, Ibáñez I, et al. Presynaptic control of glycine transporter 2 (GlyT2) by physical and functional association with plasma membrane Ca2+-ATPase (PMCA) and Na+-Ca2+ exchanger (NCX). J Biol Chem. 2014;289:34308-24 pubmed publisher
    ..After synaptic vesicle fusion, glycine is recovered back to the presynaptic terminal by the neuronal glycine transporter 2 (GlyT2) to maintain quantal glycine content in synaptic vesicles...
  7. Peña Rangel M, Riesgo Escovar J, Sánchez Chávez G, Salceda R. Glycine transporters (glycine transporter 1 and glycine transporter 2) are expressed in retina. Neuroreport. 2008;19:1295-9 pubmed publisher
    ..synaptic concentrations of glycine are regulated by at least two transporters: glycine transporter 1 and glycine transporter 2. Here, we show retinal expression of glycine transporter 1 by in-situ hybridization and of glycine ..
  8. Núñez E, Pérez Siles G, Rodenstein L, Alonso Torres P, Zafra F, Jiménez E, et al. Subcellular localization of the neuronal glycine transporter GLYT2 in brainstem. Traffic. 2009;10:829-43 pubmed publisher
    The neuronal glycine transporter GLYT2 belongs to the neurotransmitter:sodium:symporter (NSS) family and removes glycine from the synaptic cleft, thereby aiding the termination of the glycinergic signal and achieving the reloading of the ..
  9. Aubrey K, Rossi F, Ruivo R, Alboni S, Bellenchi G, Le Goff A, et al. The transporters GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype. J Neurosci. 2007;27:6273-81 pubmed
    ..We found that glycine is released from vesicles when VIAAT is coexpressed with either the neuronal transporter GlyT2 or the glial transporter GlyT1...

More Information


  1. Rees M, Harvey K, Pearce B, Chung S, Duguid I, Thomas P, et al. Mutations in the gene encoding GlyT2 (SLC6A5) define a presynaptic component of human startle disease. Nat Genet. 2006;38:801-6 pubmed
    ..8), encoding the presynaptic glycine transporter 2 (GlyT2), also cause hyperekplexia...
  2. Kitzman P. VGLUT1 and GLYT2 labeling of sacrocaudal motoneurons in the spinal cord injured spastic rat. Exp Neurol. 2007;204:195-204 pubmed
    ..cholera toxin beta-subunit (CTB), and temporal changes in vesicular glutamate transporter 1 (VGLUT1) and glycine transporter 2 (GlyT2) inputs to CTB-labeled motoneurons were visualized using antibodies specific for each synaptic type ..
  3. Liu Q, Lopez Corcuera B, Mandiyan S, Nelson H, Nelson N. Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features. J Biol Chem. 1993;268:22802-8 pubmed
    A novel glycine transporter (GLYT2) was cloned from a rat brain cDNA library. GLYT2 is about 48 and 50% homologous to the previously cloned mouse glycine transporter (GLYT1) and rat proline transporter (PROT), respectively...
  4. Pérez Siles G, Morreale A, Leo Macias A, Pita G, Ortiz A, Aragón C, et al. Molecular basis of the differential interaction with lithium of glycine transporters GLYT1 and GLYT2. J Neurochem. 2011;118:195-204 pubmed publisher
    ..The neuronal GLYT2, with higher sodium coupling (3:1 :1), supplies glycine to the pre-synaptic terminal to refill synaptic vesicles...
  5. Edington A, McKinzie A, Reynolds A, Kassiou M, Ryan R, Vandenberg R. Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonylglycine inhibition of glycine transport. J Biol Chem. 2009;284:36424-30 pubmed publisher
    ..glycine in the central nervous system are regulated by Na(+)/Cl(-)-dependent glycine transporters, GLYT1 and GLYT2. N-Arachidonylglycine (NAGly) is an endogenous inhibitor of GLYT2 with little or no effect on GLYT1 and is ..
  6. Fornés A, Núñez E, Aragón C, López Corcuera B. The second intracellular loop of the glycine transporter 2 contains crucial residues for glycine transport and phorbol ester-induced regulation. J Biol Chem. 2004;279:22934-43 pubmed
    ..In this report, we have investigated the involvement of the second intracellular loop of the neuronal glycine transporter 2 (GLYT2) on the protein conformational equilibrium and the regulation by 4alpha-phorbol 12 myristate 13-..
  7. Tanaka I, Ezure K, Kondo M. Distribution of glycine transporter 2 mRNA-containing neurons in relation to glutamic acid decarboxylase mRNA-containing neurons in rat medulla. Neurosci Res. 2003;47:139-51 pubmed
    ..neurons in relation to GABAergic neurons, by using in situ hybridization method for mRNA encoding either glycine transporter 2 (GLYT2) or glutamic acid decarboxylase isoform 67 (GAD67)...
  8. Baliova M, Betz H, Jursky F. Calpain-mediated proteolytic cleavage of the neuronal glycine transporter, GlyT2. J Neurochem. 2004;88:227-32 pubmed
    The glycine transporter 2 (GlyT2) belongs to the family of Na+/CL--dependent plasma membrane transporters and is localized on the presynaptic terminals of glycinergic neurons...
  9. Tanaka I, Ezure K. Overall distribution of GLYT2 mRNA-containing versus GAD67 mRNA-containing neurons and colocalization of both mRNAs in midbrain, pons, and cerebellum in rats. Neurosci Res. 2004;49:165-78 pubmed
    ..neurons in the midbrain, pons, and cerebellum in rats, using in situ hybridization for mRNA encoding glycine transporter 2 (GLYT2), which reliably detects glycinergic cell bodies...
  10. Aroeira R, Sebastião A, Valente C. GlyT1 and GlyT2 in brain astrocytes: expression, distribution and function. Brain Struct Funct. 2014;219:817-30 pubmed publisher
    GlyT1 and GlyT2 are the transporters responsible for glycine uptake from the synaptic cleft...
  11. Arribas González E, Alonso Torres P, Aragón C, López Corcuera B. Calnexin-assisted biogenesis of the neuronal glycine transporter 2 (GlyT2). PLoS ONE. 2013;8:e63230 pubmed publisher
    ..Mutations in the human GlyT2 gene (SLC6A5) that cause deficient glycine transport or defective GlyT2 trafficking are the second most common cause of ..
  12. Aroeira R, Sebastião A, Valente C. BDNF, via truncated TrkB receptor, modulates GlyT1 and GlyT2 in astrocytes. Glia. 2015;63:2181-97 pubmed publisher
    Glycine transporters (GlyT), GlyT1 and GlyT2, are responsible for the termination of glycine-mediated synaptic activity through removal of neurotransmitter from synaptic cleft...
  13. Ohno K, Koroll M, El Far O, Scholze P, Gomeza J, Betz H. The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1. Mol Cell Neurosci. 2004;26:518-29 pubmed
    The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space...