Gene Symbol: Slc25a4
Description: solute carrier family 25 member 4
Alias: Ant1, ADP/ATP translocase 1, ADP,ATP carrier protein 1, ANT 1, adenine nucleotide translocator 1, adenine nucleotide translocator), member 4, mitochondrial adenine nucleotide translocator, solute carrier family 25 (mitochondrial adenine nucleotide translocator) member 4, solute carrier family 25 (mitochondrial carrier, solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
Species: rat
Products:     Slc25a4

Top Publications

  1. Jacotot E, Ravagnan L, Loeffler M, Ferri K, Vieira H, Zamzami N, et al. The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore. J Exp Med. 2000;191:33-46 pubmed
    ..Hence, Vpr induces apoptosis via a direct effect on the mitochondrial PTPC. ..
  2. García N, Martínez Abundis E, Pavón N, Correa F, Chávez E. Copper induces permeability transition through its interaction with the adenine nucleotide translocase. Cell Biol Int. 2007;31:893-9 pubmed
  3. Colell A, Garcia Ruiz C, Lluis J, Coll O, Mari M, Fernandez Checa J. Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity. J Biol Chem. 2003;278:33928-35 pubmed
    ..Thus, these findings indicate that the ability of atractyloside to induce MPT via ANT requires an appropriate membrane fluidity range. ..
  4. Palmieri L, Alberio S, Pisano I, Lodi T, Meznaric Petrusa M, Zidar J, et al. Complete loss-of-function of the heart/muscle-specific adenine nucleotide translocator is associated with mitochondrial myopathy and cardiomyopathy. Hum Mol Genet. 2005;14:3079-88 pubmed
    ..found in the gene encoding the heart and skeletal muscle-specific isoform of the adenine nucleotide translocator (ANT1) in families with autosomal dominant progressive external opthalmoplegia and in a sporadic patient...
  5. Jing L, Zhou L, Li W, Zhang F, Yuan L, Li S, et al. Carnitine regulates myocardial metabolism by Peroxisome Proliferator-Activated Receptor-alpha (PPARalpha) in alcoholic cardiomyopathy. Med Sci Monit. 2011;17:BR1-9 pubmed
    ..b>Mitochondrial adenine nucleotide translocator-1 (ANT1) activity, ATPase activity, high energy phosphate concentration, peroxisome ..
  6. Amadoro G, Corsetti V, Atlante A, Florenzano F, Capsoni S, Bussani R, et al. Interaction between NH(2)-tau fragment and A? in Alzheimer's disease mitochondria contributes to the synaptic deterioration. Neurobiol Aging. 2012;33:833.e1-25 pubmed publisher
    ..protein-preferentially interacts with A? peptide(s) in human AD synapses in association with mitochondrial adenine nucleotide translocator-1 (ANT-1) and cyclophilin D...
  7. Chiara F, Castellaro D, Marin O, Petronilli V, BRUSILOW W, Juhaszova M, et al. Hexokinase II detachment from mitochondria triggers apoptosis through the permeability transition pore independent of voltage-dependent anion channels. PLoS ONE. 2008;3:e1852 pubmed publisher
    ..These findings have profound implications for our understanding of the pathways of outer mitochondrial membrane permeabilization and their inactivation in tumors. ..
  8. Liang X, Huang J, Lin X, Qin F, Wen Q, Chen C, et al. The effect of 17-methoxyl-7-hydroxy-benzene-furanchalcone on NF-?B and the inflammatory response during myocardial ischemia reperfusion injury in rats. J Cardiovasc Pharmacol. 2014;63:68-75 pubmed publisher
    ..The experiment showed that MHBFC protected the heart against MI/RI possibly by reducing lipid peroxidation damage while inhibiting the activity of NF-?Bp65 and the inflammatory response. ..
  9. Lin D, Lechleiter J. Mitochondrial targeted cyclophilin D protects cells from cell death by peptidyl prolyl isomerization. J Biol Chem. 2002;277:31134-41 pubmed
    ..Collectively, our data suggest that CyPD should be viewed as a cell survival-signaling molecule and indicate a protective role of CyPD against apoptosis that is mediated by one or more targets other than the ANT. ..

More Information


  1. Queiroga C, Almeida A, Martel C, Brenner C, Alves P, Vieira H. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilization and apoptosis. J Biol Chem. 2010;285:17077-88 pubmed publisher
    ..In conclusion, CO protects astrocytes from apoptosis by preventing MMP, acting on ANT (glutathionylation and inhibition of its pore activity) via a preconditioning-like process mediated by ROS and GSSG. ..
  2. Klumpe I, Savvatis K, Westermann D, Tschöpe C, Rauch U, Landmesser U, et al. Transgenic overexpression of adenine nucleotide translocase 1 protects ischemic hearts against oxidative stress. J Mol Med (Berl). 2016;94:645-53 pubmed publisher
    ..We investigated whether ANT1 overexpression has protective effects on ischemic hearts...
  3. Korotkov S, Konovalova S, Brailovskaya I, Saris N. To involvement the conformation of the adenine nucleotide translocase in opening the Tl(+)-induced permeability transition pore in Ca(2+)-loaded rat liver mitochondria. Toxicol In Vitro. 2016;32:320-32 pubmed publisher
    ..Using the Tl(+)-induced MPTP model is discussed in terms finding new transition pore inhibitors and inducers among different chemical and natural compounds. ..
  4. Deschauer M, Hudson G, Müller T, Taylor R, Chinnery P, Zierz S. A novel ANT1 gene mutation with probable germline mosaicism in autosomal dominant progressive external ophthalmoplegia. Neuromuscul Disord. 2005;15:311-5 pubmed
    Only four different mutations in the adenine nucleotide translocator 1 (ANT1) gene have been found in families with progressive external ophthalmoplegia (PEO)...
  5. Temkin V, Huang Q, Liu H, Osada H, Pope R. Inhibition of ADP/ATP exchange in receptor-interacting protein-mediated necrosis. Mol Cell Biol. 2006;26:2215-25 pubmed
    ..These observations demonstrate a novel mechanism initiated through death receptor ligation and mediated by RIP that results in the suppression of ANT activity and necrosis. ..
  6. Nicoletti V, Marino V, Cuppari C, Licciardello D, Patti D, Purrello V, et al. Effect of antioxidant diets on mitochondrial gene expression in rat brain during aging. Neurochem Res. 2005;30:737-52 pubmed
    ..The observed age-related changes of expression were prevented by the dietary treatments. The present study provides further evidence for the critical role of mitochondria in the aging process. ..
  7. Heger J, Abdallah Y, Shahzad T, Klumpe I, Piper H, Schultheiss H, et al. Transgenic overexpression of the adenine nucleotide translocase 1 protects cardiomyocytes against TGF?1-induced apoptosis by stabilization of the mitochondrial permeability transition pore. J Mol Cell Cardiol. 2012;53:73-81 pubmed publisher
    Since adenine nucleotide translocase 1 (ANT1) overexpression improved cardiac function in rats with activated renin-angiotensin system (RAS) and angiotensin II is known to enhance transforming growth factor ? (TGF?) signaling in ..
  8. Rardin M, Wiley S, Murphy A, Pagliarini D, Dixon J. Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane. J Biol Chem. 2008;283:15440-50 pubmed publisher
    ..This work rigorously demonstrates the unique location of two highly similar DSPs on opposing sides of the mitochondrial inner membrane...
  9. Teodoro J, Rolo A, Oliveira P, Palmeira C. Decreased ANT content in Zucker fatty rats: relevance for altered hepatic mitochondrial bioenergetics in steatosis. FEBS Lett. 2006;580:2153-7 pubmed
    ..These data suggest that hepatic accumulation of fat impairs mitochondrial function, reflecting the loss of oxidative phosphorylation capacity caused by a decrease in the ANT content. ..
  10. Ledesma M, Da Silva J, Schevchenko A, Wilm M, Dotti C. Proteomic characterisation of neuronal sphingolipid-cholesterol microdomains: role in plasminogen activation. Brain Res. 2003;987:107-16 pubmed
    ..These observations suggest that neuronal rafts may play, in addition to intracellular signaling, a role in extracellular/membrane protein proteolysis. ..
  11. Tang C, Sula M, Bohnet S, Rehman A, Taishi P, Krueger J. Interleukin-1beta induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP. Brain Res Mol Brain Res. 2005;137:213-22 pubmed
    ..We conclude that rat CBP shares many of the functional domains as human and murine CBP and that IL-1 upregulates genes previously associated with learning and sleep. ..
  12. Bobba A, Amadoro G, Azzariti A, Pizzuto R, Atlante A. Extracellular ADP prevents neuronal apoptosis via activation of cell antioxidant enzymes and protection of mitochondrial ANT-1. Biochim Biophys Acta. 2014;1837:1338-49 pubmed publisher
    ..e. A?1-42 and NH2htau, which are known to be produced in apoptotic cerebellar neurons, further corroborate the molecular mechanism of neuroprotection by ADP, herein proposed. ..
  13. Thompson K, Majd H, Dallabona C, Reinson K, King M, Alston C, et al. Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number. Am J Hum Genet. 2016;99:860-876 pubmed publisher
    Mutations in SLC25A4 encoding the mitochondrial ADP/ATP carrier AAC1 are well-recognized causes of mitochondrial disease...
  14. Laoudj Chenivesse D, Carnac G, Bisbal C, Hugon G, Bouillot S, Desnuelle C, et al. Increased levels of adenine nucleotide translocator 1 protein and response to oxidative stress are early events in facioscapulohumeral muscular dystrophy muscle. J Mol Med (Berl). 2005;83:216-24 pubmed
    ..any of the 4q35 genes, among which is the heart and muscle-specific isoform of the adenine nucleotide translocator (ANT1)...
  15. Vogelpohl I, Vetter R, Heger J, Ebermann L, Euler G, Schultheiss H, et al. Transgenic overexpression of heart-specific adenine nucleotide translocase 1 positively affects contractile function in cardiomyocytes. Cell Physiol Biochem. 2011;27:121-8 pubmed publisher
    ..Interestingly, myocardial ANT1 overexpression preserves cardiac structure and function under pathophysiological conditions...
  16. Shinohara Y, Kamida M, Yamazaki N, Terada H. Isolation and characterization of cDNA clones and a genomic clone encoding rat mitochondrial adenine nucleotide translocator. Biochim Biophys Acta. 1993;1152:192-6 pubmed
    Two cDNA clones encoding rat mitochondrial adenine nucleotide translocator were isolated from libraries constructed from mRNAs of heart and liver...
  17. Winter J, Klumpe I, Heger J, Rauch U, Schultheiss H, Landmesser U, et al. Adenine nucleotide translocase 1 overexpression protects cardiomyocytes against hypoxia via increased ERK1/2 and AKT activation. Cell Signal. 2016;28:152-9 pubmed publisher
    ..In this regard, we analysed the effect of adenine nucleotide translocase 1 (ANT1), which facilitates the exchange of ADP and ATP across the mitochondrial membrane, on cell-protective survival ..
  18. Parker N, Affourtit C, Vidal Puig A, Brand M. Energization-dependent endogenous activation of proton conductance in skeletal muscle mitochondria. Biochem J. 2008;412:131-9 pubmed publisher
  19. Buck C, Jurynec M, Gupta D, Law A, Bilger J, Wallace D, et al. Increased adenine nucleotide translocator 1 in reactive astrocytes facilitates glutamate transport. Exp Neurol. 2003;181:149-58 pubmed
    ..we employed an in vivo model of the glial scar and differential display PCR and found an increase in the level of Ant1, a mitochondrial ATP/ADP exchanger that facilitates the flux of ATP out of the mitochondria...
  20. Siciliano G, Tessa A, Petrini S, Mancuso M, Bruno C, Grieco G, et al. Autosomal dominant external ophthalmoplegia and bipolar affective disorder associated with a mutation in the ANT1 gene. Neuromuscul Disord. 2003;13:162-5 pubmed
    ..The authors identified a missense mutation (leucine 98-->proline) in the adenine nucleotide translocator 1 gene...
  21. Manchado C, Orús J, Villarroya F, Roig E, Heras M, Giralt M, et al. Epitope mapping of mitochondrial adenine nucleotide translocase-1 in idiopathic dilated cardiomyopathy. J Mol Cell Cardiol. 2002;34:571-82 pubmed
    ..ANT in IDCM by immunoblot with recombinant GST-ANT fusion proteins and with cellulose-bound decapeptides of human ANT1. Forty-five patients with IDCM, 17 patients with ischemic left ventricle dysfunction (LVD) and 20 controls were ..
  22. Cione E, Pingitore A, Genchi F, Genchi G. Coenzyme A enhances activity of the mitochondrial adenine nucleotide translocator. Int J Biochem Cell Biol. 2010;42:106-12 pubmed publisher
    ..50+/-0.52 microM versus 22.19+/-0.98 microM). Data suggest a likely enzymatic involvement in the interaction between ANT and CoA. The effect of CoA is observed in mitochondria from several different tissues. ..
  23. Walther T, Tschope C, Sterner Kock A, Westermann D, Heringer Walther S, Riad A, et al. Accelerated mitochondrial adenosine diphosphate/adenosine triphosphate transport improves hypertension-induced heart disease. Circulation. 2007;115:333-44 pubmed
    ..mitochondrial ATP/ADP transportation on cardiac dysfunction, we generated transgenic rats overexpressing ANT1 in the heart (ANT rats) and crossed them with renin-overexpressing rats (REN rats) suffering from hypertension-..
  24. Skarka L, Bardova K, Brauner P, Flachs P, Jarkovská D, Kopecky J, et al. Expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in developing rat heart: putative involvement in control of mitochondrial membrane potential. J Mol Cell Cardiol. 2003;35:321-30 pubmed
    ..alpha (PPARalpha) genes was characterized by northern blotting (UCP2), real-time quantitative RT-PCR (UCP2, UCP3, ANT1, ANT2, and PPARalpha), and by immunoblotting (UCP3)...
  25. Jacotot E, Ferri K, El Hamel C, Brenner C, Druillennec S, Hoebeke J, et al. Control of mitochondrial membrane permeabilization by adenine nucleotide translocator interacting with HIV-1 viral protein rR and Bcl-2. J Exp Med. 2001;193:509-19 pubmed
    ..Concomitantly, Bcl-2 suppresses channel formation by the ANT-Vpr complex in synthetic membranes. In conclusion, both Vpr and Bcl-2 modulate MMP through a direct interaction with ANT. ..
  26. Esposito L, Melov S, Panov A, Cottrell B, Wallace D. Mitochondrial disease in mouse results in increased oxidative stress. Proc Natl Acad Sci U S A. 1999;96:4820-5 pubmed
    ..we examined the mitochondria from mice lacking the heart/muscle isoform of the adenine nucleotide translocator (Ant1), designated Ant1(tm2Mgr) (-/-) mice...
  27. Woodfield K, Ruck A, Brdiczka D, Halestrap A. Direct demonstration of a specific interaction between cyclophilin-D and the adenine nucleotide translocase confirms their role in the mitochondrial permeability transition. Biochem J. 1998;336 ( Pt 2):287-90 pubmed
    ..Binding was prevented by pretreatment of the CyP-D with CsA, but not with cyclosporin H. Purified ANT also bound specifically to GST-CyP-D, but porin did not, even in the presence of ANT. ..
  28. Brandolin G, Le Saux A, Trezeguet V, Lauquin G, Vignais P. Chemical, immunological, enzymatic, and genetic approaches to studying the arrangement of the peptide chain of the ADP/ATP carrier in the mitochondrial membrane. J Bioenerg Biomembr. 1993;25:459-72 pubmed
    ..Mutagenesis in yeast is currently being used to detect strategic amino acids in ADP/ATP transport. ..
  29. Hang T, Huang Z, Jiang S, Gong J, Wang C, Xie D, et al. Apoptosis in pressure overload-induced cardiac hypertrophy is mediated, in part, by adenine nucleotide translocator-1. Ann Clin Lab Sci. 2006;36:88-95 pubmed
    This study explored the role of adenine nucleotide translocator-1 (ANT1) in cardiomyocyte apoptosis during left ventricular hypertrophy (LVH) that developed in response to pressure overload...
  30. Grado A, Manchado C, Iglesias R, Giralt M, Villarroya F, Mampel T, et al. Muscle/heart isoform of mitochondrial adenine nucleotide translocase (ANT1) is transiently expressed during perinatal development in rat liver. FEBS Lett. 1998;421:213-6 pubmed
    ..order to define the contribution of the ANT isoforms to this postnatal increase we have studied the expression of ANT1 and ANT2 isoforms in the liver during this period...