Slc22a6

Summary

Gene Symbol: Slc22a6
Description: solute carrier family 22 member 6
Alias: Oat1, Orctl1, Paht, Roat1, solute carrier family 22 member 6, organic anion transporter 1, organic cationic transporter-like 1, rROAT1, renal organic anion transporter 1, solute carrier family 22 (organic anion transporter), member 6
Species: rat
Products:     Slc22a6

Top Publications

  1. Sweet D, Wolff N, Pritchard J. Expression cloning and characterization of ROAT1. The basolateral organic anion transporter in rat kidney. J Biol Chem. 1997;272:30088-95 pubmed
    ..containing a 1,656-base pair open reading frame coding for a peptide 551 amino acids long was isolated and named ROAT1. ROAT1-mediated transport of 50 mu M [3H]PAH was independent of imposed changes in membrane potential...
  2. Torres A, Dnyanmote A, Bush K, Wu W, Nigam S. Deletion of multispecific organic anion transporter Oat1/Slc22a6 protects against mercury-induced kidney injury. J Biol Chem. 2011;286:26391-5 pubmed publisher
    ..Here, we investigated the in vivo role of Oat1 (organic anion transporter 1; originally NKT (Lopez-Nieto, C. E., You, G., Bush, K. T., Barros, E. J., Beier, D. R., and Nigam, S. K...
  3. Pavlova A, Sakurai H, Leclercq B, Beier D, Yu A, Nigam S. Developmentally regulated expression of organic ion transporters NKT (OAT1), OCT1, NLT (OAT2), and Roct. Am J Physiol Renal Physiol. 2000;278:F635-43 pubmed
    ..When we initially identified NKT, also known as OAT1, the first member of the organic anion transporter (OAT) family (Lopez-Nieto CE, You G, Bush KT, Barros EJ, Beier ..
  4. Uwai Y, Honjo E. Transport of xanthurenic acid by rat/human organic anion transporters OAT1 and OAT3. Biosci Biotechnol Biochem. 2013;77:1517-21 pubmed
    ..Our laboratory previously described its transport by rat/human organic anion transporters rOAT1, hOAT1, rOAT3 and hOAT3, which are involved in drug disposition...
  5. Denk G, Soroka C, Mennone A, Koepsell H, Beuers U, Boyer J. Down-regulation of the organic cation transporter 1 of rat liver in obstructive cholestasis. Hepatology. 2004;39:1382-9 pubmed
    ..9% of controls (P <.0005). In conclusion, obstructive cholestasis down-regulates Oct1 and impairs Oct1-mediated uptake in rat liver, suggesting that hepatic uptake of small cationic drugs may be impaired in cholestatic liver injury. ..
  6. Erman F, Tuzcu M, Orhan C, Sahin N, Sahin K. Effect of lycopene against cisplatin-induced acute renal injury in rats: organic anion and cation transporters evaluation. Biol Trace Elem Res. 2014;158:90-5 pubmed publisher
    ..1.37 mg/dl) levels and the kidney efflux transporters MRP2 and MRP4 levels were significantly increased, whereas OAT1, OAT3, OCT1, and OCT2 levels in kidney were decreased in the treated rats compared with normal control rats...
  7. Jiang W, Prokopenko O, Wong L, Inouye M, Mirochnitchenko O. IRIP, a new ischemia/reperfusion-inducible protein that participates in the regulation of transporter activity. Mol Cell Biol. 2005;25:6496-508 pubmed
    ..The activities of exogenous organic cation transporters (OCT2 and OCT3), organic anion transporter (OAT1), and monoamine transporters were also inhibited by IRIP...
  8. Barros S, Srimaroeng C, Perry J, Walden R, Dembla Rajpal N, Sweet D, et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem. 2009;284:2672-9 pubmed publisher
    ..Increased ES transport was not observed in renal slices from OAT3-null mice. Transport of the shared OAT1/OAT3 substrate, rho-aminohippurate, behaved similarly, except that stimulation was reduced, not abolished, in the ..
  9. Ekaratanawong S, Anzai N, Jutabha P, Miyazaki H, Noshiro R, Takeda M, et al. Human organic anion transporter 4 is a renal apical organic anion/dicarboxylate exchanger in the proximal tubules. J Pharmacol Sci. 2004;94:297-304 pubmed

More Information

Publications42

  1. Beery E, Middel P, Bahn A, Willenberg H, Hagos Y, Koepsell H, et al. Molecular evidence of organic ion transporters in the rat adrenal cortex with adrenocorticotropin-regulated zonal expression. Endocrinology. 2003;144:4519-26 pubmed
    ..into, bovine adrenocortical cells showed properties of the renal p-[3H]aminohippurate/anion exchanger OAT1. Other poly-specific transporters such as organic anion-transporting polypeptides (oatps) and organic cation ..
  2. Thiebaud N, Menetrier F, Belloir C, Minn A, Neiers F, Artur Y, et al. Expression and differential localization of xenobiotic transporters in the rat olfactory neuro-epithelium. Neurosci Lett. 2011;505:180-5 pubmed publisher
    ..The pattern of expression and the distinct localization of the olfactory transporters showed in this work may highlight on their specific function in the whole olfactory epithelium...
  3. Gallegos T, Martovetsky G, Kouznetsova V, Bush K, Nigam S. Organic anion and cation SLC22 "drug" transporter (Oat1, Oat3, and Oct1) regulation during development and maturation of the kidney proximal tubule. PLoS ONE. 2012;7:e40796 pubmed publisher
    ..Particularly important are the SLC22 family of transporters, including the organic anion transporters Oat1 (originally identified as NKT) and Oat3 as well as the organic cation transporter Oct1...
  4. Schneider R, Meusel M, Betz B, Held C, Möller Ehrlich K, Büttner Herold M, et al. Oat1/3 restoration protects against renal damage after ischemic AKI. Am J Physiol Renal Physiol. 2015;308:F198-208 pubmed publisher
    Expression of proximal tubular organic anion transporters Oat1 and Oat3 is reduced by PGE2 after renal ischemia and reperfusion (I/R) injury...
  5. Villar S, Brandoni A, Torres A. Time course of organic anion excretion in rats with bilateral ureteral obstruction: role of organic anion transporters (Oat1 and Oat3). Nephron Physiol. 2008;110:p45-56 pubmed publisher
    ..time course of P-aminohippurate (PAH) renal excretion and the cortical expression of organic anion transporters (Oat1 and Oat3) at 1 (BUO-1), 2 (BUO-2) and 7 (BUO-7) days after release of 24-hour bilateral ureteral obstruction (BUO) ..
  6. Lu R, Chan B, Schuster V. Cloning of the human kidney PAH transporter: narrow substrate specificity and regulation by protein kinase C. Am J Physiol. 1999;276:F295-303 pubmed
    ..Several structurally diverse organic anions cis-inhibited PAH uptake. Like rat OAT1 organic anion transporter, hPAHT was inhibited by furosemide, indomethacin, probenecid, and alpha-ketoglutarate...
  7. Shibayama Y, Ushinohama K, Ikeda R, Yoshikawa Y, Motoya T, Takeda Y, et al. Effect of methotrexate treatment on expression levels of multidrug resistance protein 2, breast cancer resistance protein and organic anion transporters Oat1, Oat2 and Oat3 in rats. Cancer Sci. 2006;97:1260-6 pubmed
    ..The levels of Mrp2, Oat1 and Oat2 on day 1 after the treatment showed no significant change...
  8. Sekine T, Watanabe N, Hosoyamada M, Kanai Y, Endou H. Expression cloning and characterization of a novel multispecific organic anion transporter. J Biol Chem. 1997;272:18526-9 pubmed
    ..report on the isolation of a novel complementary DNA from rat kidney that encodes a 551-amino acid residue protein (OAT1) with 12 putative membrane-spanning domains...
  9. Deguchi T, Kusuhara H, Takadate A, Endou H, Otagiri M, Sugiyama Y. Characterization of uremic toxin transport by organic anion transporters in the kidney. Kidney Int. 2004;65:162-74 pubmed
    ..IS has been shown to be a substrate of rat organic anion transporter 1 (rOat1) and rOat3...
  10. Liu T, Meng Q, Wang C, Liu Q, Guo X, Sun H, et al. Changes in expression of renal Oat1, Oat3 and Mrp2 in cisplatin-induced acute renal failure after treatment of JBP485 in rats. Toxicol Appl Pharmacol. 2012;264:423-30 pubmed publisher
    ..JBP485) on acute renal failure (ARF) induced by cisplatin is related to change in expression of renal Oat1, Oat3 and Mrp2 in rats...
  11. Xu D, Wang H, Gardner C, Pan Z, Zhang P, Zhang J, et al. The role of Nedd4-1 WW domains in binding and regulating human organic anion transporter 1. Am J Physiol Renal Physiol. 2016;311:F320-9 pubmed publisher
    Human organic anion transporter 1 (hOAT1), expressed at the basolateral membrane of kidney proximal tubule cells, mediates the active renal secretion of a diverse array of clinically important drugs, including anti-human immunodeficiency ..
  12. Keller T, Schwarz D, Bernhard F, Dötsch V, Hunte C, Gorboulev V, et al. Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry. 2008;47:4552-64 pubmed publisher
    ..Here, we synthesized rat organic cation transporters OCT1 and OCT2 and rat organic anion transporter OAT1 in a cell free system in the absence of detergent...
  13. Bulacio R, Hazelhoff M, Torres A. Renal expression and function of oat1 and oat3 in rats with vascular calcification. Pharmacology. 2012;90:66-77 pubmed publisher
    ..The organic anion transporters 1 and 3 (Oat1 and Oat3) are present in renal basolateral membranes, which transport organic anions of pharmacological and ..
  14. Kobayashi Y, Hirokawa N, Ohshiro N, Sekine T, Sasaki T, Tokuyama S, et al. Differential gene expression of organic anion transporters in male and female rats. Biochem Biophys Res Commun. 2002;290:482-7 pubmed
    Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA...
  15. Schneider R, Sauvant C, Betz B, Otremba M, Fischer D, Holzinger H, et al. Downregulation of organic anion transporters OAT1 and OAT3 correlates with impaired secretion of para-aminohippurate after ischemic acute renal failure in rats. Am J Physiol Renal Physiol. 2007;292:F1599-605 pubmed
    ..This process is mediated by the organic anion transporters OAT1 and OAT3, which both have a broad spectrum of substrates including a variety of pharmaceutics and toxins...
  16. Brandoni A, Anzai N, Kanai Y, Endou H, Torres A. Renal elimination of p-aminohippurate (PAH) in response to three days of biliary obstruction in the rat. The role of OAT1 and OAT3. Biochim Biophys Acta. 2006;1762:673-82 pubmed
    ..of serum bile acids and bilirubin) on the systemic and renal PAH clearance and on the expression of cortical renal OAT1 and OAT3 in a rat model. PAH is the prototypical substrate of the renal organic anion transport system...
  17. Kwak J, Kim H, Oh K, Kim D, Han K, Cha S. Co-localization and interaction of organic anion transporter 1 with caveolin-2 in rat kidney. Exp Mol Med. 2005;37:204-12 pubmed
    ..The rat OAT1 (rOAT1) transports a number of negatively charged organic compounds between the cells and their extracellular milieu...
  18. Nakakariya M, Shima Y, Shirasaka Y, Mitsuoka K, Nakanishi T, Tamai I. Organic anion transporter OAT1 is involved in renal handling of citrulline. Am J Physiol Renal Physiol. 2009;297:F71-9 pubmed publisher
    ..In studies using transporter-overexpressing cells, human organic anion transporter 1 (OAT1) and rat Oat1 exhibited citrulline transport activity with K(m) values of 238 and 373 microM, ..
  19. Bahn A, Hauss A, Appenroth D, Ebbinghaus D, Hagos Y, Steinmetzer P, et al. RT-PCR-based evidence for the in vivo stimulation of renal tubularp-aminohippurate (PAH) transport by triiodothyronine (T3) or dexamethasone (DEXA) in kidney tissue of immature and adult rats. Exp Toxicol Pathol. 2003;54:367-73 pubmed
    ..the stimulation of PAH accumulation in renal cortical slices as well as the expression level of organic anion transporter 1 (OAT1), the recently cloned renal basolateral PAH-transporter, using RT-PCR techniques under the ..
  20. Hu E, Chen Z, Fredrickson T, Spurr N, Gentle S, Sims M, et al. Rapid isolation of tissue-specific genes from rat kidney. Exp Nephrol. 2001;9:156-64 pubmed
    ..These included three renal-specific transporters (an ADH water channel, the anion transporters RST and ROAT1), a cell adhesion molecule (K-cadherin) and a kidney-specific protein upregulated in renal carcinoma (DD96)...
  21. Youngblood G, Sweet D. Identification and functional assessment of the novel murine organic anion transporter Oat5 (Slc22a19) expressed in kidney. Am J Physiol Renal Physiol. 2004;287:F236-44 pubmed
    ..Slc22a19 was localized to mouse chromosome 19 near the genes encoding Oat1 (Slc22a6) and Oat3 (Slc22a8)...
  22. Matsuzaki T, Watanabe H, Yoshitome K, Morisaki T, Hamada A, Nonoguchi H, et al. Downregulation of organic anion transporters in rat kidney under ischemia/reperfusion-induced acute [corrected] renal failure. Kidney Int. 2007;71:539-47 pubmed
    ..However, this increase was significantly attenuated by ingestion of cobalt. The level of mRNA and protein of both rOAT1 and rOAT3 were markedly depressed in the ischemic kidney...
  23. Villar S, Brandoni A, Anzai N, Endou H, Torres A. Altered expression of rat renal cortical OAT1 and OAT3 in response to bilateral ureteral obstruction. Kidney Int. 2005;68:2704-13 pubmed
    ..was to study the renal excretion of p-aminohippurate (PAH) and the cortical renal expression of the organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) in BUO rats...
  24. Komazawa H, Yamaguchi H, Hidaka K, Ogura J, Kobayashi M, Iseki K. Renal uptake of substrates for organic anion transporters Oat1 and Oat3 and organic cation transporters Oct1 and Oct2 is altered in rats with adenine-induced chronic renal failure. J Pharm Sci. 2013;102:1086-94 pubmed publisher
    ..clearances of benzylpenicillin and metformin, which are typical substrates for renal organic anion transporters Oat1 and Oat3 and organic cation transporters Oct1 and Oct2, respectively, were evaluated...
  25. Brandoni A, Torres A. Altered Renal Expression of Relevant Clinical Drug Transporters in Different Models of Acute Uremia in Rats. Role of Urea Levels. Cell Physiol Biochem. 2015;36:907-16 pubmed publisher
    b>Organic anion transporter 1 (Oat1) and 3 (Oat3) are organic anion transporters that play critical roles in the body disposition of numerous clinically important drugs...
  26. Hosoyamada M, Sekine T, Kanai Y, Endou H. Molecular cloning and functional expression of a multispecific organic anion transporter from human kidney. Am J Physiol. 1999;276:F122-8 pubmed
    Recently, we isolated the multispecific organic anion transporter (OAT1) from the rat kidney, which plays important roles in the renal elimination of endogenous and exogenous organic anions including clinically important drugs...
  27. Kittayaruksakul S, Soodvilai S, Asavapanumas N, Muanprasat C, Chatsudthipong V. Liver X receptor activation downregulates organic anion transporter 1 (OAT1) in the renal proximal tubule. Am J Physiol Renal Physiol. 2012;302:F552-60 pubmed publisher
    ..In this study, we investigated the role of LXRs in the regulation of human organic anion transporter 1 (hOAT1), a major transporter localized in the basolateral membrane of the renal proximal tubule...
  28. Hocherl K, Schmidt C, Bucher M. COX-2 inhibition attenuates endotoxin-induced downregulation of organic anion transporters in the rat renal cortex. Kidney Int. 2009;75:373-80 pubmed publisher
    ..Endotoxemia caused a time- and dose-dependent decrease of OAT1 and OAT3 expression that paralleled increased renocortical COX-2 expression and PGE2 formation...
  29. Kim Y, Kim I, Noh C, Quach H, Yoon I, Chow E, et al. Effects of 1α,25-dihydroxyvitamin D3 , the natural vitamin D receptor ligand, on the pharmacokinetics of cefdinir and cefadroxil, organic anion transporter substrates, in rat. J Pharm Sci. 2014;103:3793-3805 pubmed publisher
    ..Rather, reduction of rOAT1/rOAT3 mRNA expression in kidney with 1,25(OH)2 D3 -treatment was observed, confirmed by decreased function in ..
  30. Tanaka K, Xu W, Zhou F, You G. Role of glycosylation in the organic anion transporter OAT1. J Biol Chem. 2004;279:14961-6 pubmed
    ..In summary, we provided the evidence that 1) Asp-39 is crucially involved in substrate recognition of OAT1, 2) glycosylation at individual sites is not required for OAT1 function, and 3) glycosylation plays an important ..
  31. Race J, Grassl S, Williams W, Holtzman E. Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3). Biochem Biophys Res Commun. 1999;255:508-14 pubmed
    The cloned organic anion transporters from rat, mouse, and winter flounder (rOAT1, mOAT1, fROAT) mediate the coupled exchange of alpha-ketoglutarate with multiple organic anions, including p-aminohippurate (PAH)...
  32. Wu X, Liu J, Zhang J, Liu H, Yan M, Liang B, et al. Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy. Mol Med Rep. 2016;13:2385-92 pubmed publisher
    ..It was additionally identified that in UAN rats the surface OAT1 expression levels were reduced...
  33. Severin M, Hazelhoff M, Bulacio R, Mamprin M, Brandoni A, Torres A. Impact of the induced organic anion transporter 1 (Oat1) renal expression by furosemide on the pharmacokinetics of organic anions. Nephrology (Carlton). 2017;22:642-648 pubmed publisher
    ..This study was undertaken to simultaneously evaluate the effects of furosemide pretreatment on organic anion transporter 1 (Oat1) and multidrug resistance protein 2 (Mrp2) renal expressions, on p-aminohippurate (PAH) ..