Slc12a2

Summary

Gene Symbol: Slc12a2
Description: solute carrier family 12 member 2
Alias: Bsc2, Nkcc1, solute carrier family 12 member 2, solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters), member 2
Species: rat
Products:     Slc12a2

Top Publications

  1. Fu L, Wong J, Schneider E, Thomason D. Unique 5'-end of a Na(+)-K(+)-2Cl- cotransporter-like mRNA expressed in rat skeletal muscle. DNA Seq. 1999;10:127-32 pubmed
    ..Therefore, it appears that skeletal muscle does express a Na(+)-K(+)-2Cl- cotransporter-like mRNA that may code for a protein with atypical Na(+)-K(+)-2Cl- cotransporter properties. ..
  2. Beck J, Lenart B, Kintner D, Sun D. Na-K-Cl cotransporter contributes to glutamate-mediated excitotoxicity. J Neurosci. 2003;23:5061-8 pubmed
    We hypothesized that cation-dependent Cl- transport protein Na-K-Cl cotransporter isoform 1 (NKCC1) plays a role in the disruption of ion homeostasis in cerebral ischemia...
  3. Okabe A, Ohno K, Toyoda H, Yokokura M, Sato K, Fukuda A. Amygdala kindling induces upregulation of mRNA for NKCC1, a Na(+), K(+)-2Cl(-) cotransporter, in the rat piriform cortex. Neurosci Res. 2002;44:225-9 pubmed
    ..Activity-dependent increases were observed in the mRNA for NKCC1, an inwardly-directed Cl(-) transporter, in the piriform cortex...
  4. Dzhala V, Talos D, Sdrulla D, Brumback A, Mathews G, Benke T, et al. NKCC1 transporter facilitates seizures in the developing brain. Nat Med. 2005;11:1205-13 pubmed
    ..The Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) facilitates the accumulation of Cl(-) in neurons...
  5. Yamada J, Okabe A, Toyoda H, Kilb W, Luhmann H, Fukuda A. Cl- uptake promoting depolarizing GABA actions in immature rat neocortical neurones is mediated by NKCC1. J Physiol. 2004;557:829-41 pubmed
    ..The mRNA expression levels were positively (the Cl(-) accumulating Na(+),K(+)-2Cl(-) cotransporter NKCC1) or negatively (the Cl(-) extruding K(+)-Cl(-) cotransporter KCC2) correlated with [Cl(-)](i)...
  6. Morales Aza B, Chillingworth N, Payne J, Donaldson L. Inflammation alters cation chloride cotransporter expression in sensory neurons. Neurobiol Dis. 2004;17:62-9 pubmed
    ..We have investigated the expression of the Na-K-Cl, and K-Cl cotransporters NKCC1 and KCC2, in dorsal root ganglion (DRG) and spinal sensory neurons during arthritis, a condition in which neuronal ..
  7. Ponce Coria J, Markadieu N, Austin T, Flammang L, Rios K, Welling P, et al. A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters. J Biol Chem. 2014;289:17680-8 pubmed publisher
    Na(+)-dependent chloride cotransporters (NKCC1, NKCC2, and NCC) are activated by phosphorylation to play critical roles in diverse physiological responses, including renal salt balance, hearing, epithelial fluid secretion, and volume ..
  8. López Álvarez V, Mòdol L, Navarro X, Cobianchi S. Early increasing-intensity treadmill exercise reduces neuropathic pain by preventing nociceptor collateral sprouting and disruption of chloride cotransporters homeostasis after peripheral nerve injury. Pain. 2015;156:1812-25 pubmed publisher
    ..Injury also induced Na⁺-K⁺-2Cl⁻ cotransporter 1 (NKCC1) upregulation in DRG, and K⁺-Cl⁻ cotransporter 2 (KCC2) downregulation in lumbar spinal cord dorsal horn...
  9. Schomberg S, Bauer J, Kintner D, Su G, Flemmer A, Forbush B, et al. Cross talk between the GABA(A) receptor and the Na-K-Cl cotransporter is mediated by intracellular Cl-. J Neurophysiol. 2003;89:159-67 pubmed
    ..immature neurons depends on a high intracellular Cl(-) concentration maintained by Na-K-Cl cotransporter isoform1 (NKCC1). We previously found that activation of the GABA(A) receptor led to stimulation of NKCC1...

More Information

Publications77

  1. Chabwine J, Talavera K, Van Den Bosch L, Callewaert G. NKCC1 downregulation induces hyperpolarizing shift of GABA responsiveness at near term fetal stages in rat cultured dorsal root ganglion neurons. BMC Neurosci. 2015;16:41 pubmed publisher
    ..In embryonic neurons Na-K-2Cl (NKCC1) cotransporters mediate active chloride uptake, thus increasing the intracellular chloride concentration ..
  2. Nezu A, Parvin M, Turner R. A conserved hydrophobic tetrad near the C terminus of the secretory Na+-K+-2Cl- cotransporter (NKCC1) is required for its correct intracellular processing. J Biol Chem. 2009;284:6869-76 pubmed publisher
    ..a hydrophobic amino acid tetrad (ILLV) close to the C terminus of the secretory Na+-K+-2Cl- cotransporter (NKCC1) that is important for the proper intracellular processing of this protein...
  3. Piechotta K, Lu J, Delpire E. Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1). J Biol Chem. 2002;277:50812-9 pubmed
    ..proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1) with the cotransporters KCC3, NKCC1, and NKCC2 but not KCC1 and KCC4...
  4. Jantzie L, Hu M, Park H, Jackson M, Yu J, Maxwell J, et al. Chloride cotransporter NKCC1 inhibitor bumetanide protects against white matter injury in a rodent model of periventricular leukomalacia. Pediatr Res. 2015;77:554-62 pubmed publisher
    Periventricular leukomalacia (PVL) is a major form of preterm brain injury. Na(+)-K(+)-Cl(-) 1 cotransporter (NKCC1) expression on neurons and astrocytes is developmentally regulated and mediates Cl(-) reversal potential...
  5. Parvin M, Turner R. Identification of key residues involved in the dimerization of the secretory Na(+)-K(+)-2Cl(-) cotransporter NKCC1. Biochemistry. 2011;50:9857-64 pubmed publisher
    The "secretory" Na(+)-K(+)-2Cl(-) cotransporter, NKCC1, belongs to the SLC12 gene family of electroneutral cation-chloride cotransporters...
  6. Nakanishi K, Yamada J, Takayama C, Oohira A, Fukuda A. NKCC1 activity modulates formation of functional inhibitory synapses in cultured neocortical neurons. Synapse. 2007;61:138-49 pubmed
    ..The higher [Cl(-)](i) in immature neurons is thought to be attributed to the uptake of Cl(-) mediated by NKCC1 (Na(+), K(+)-2Cl(-) cotransporter)...
  7. Anzai N, Izumida I, Kobayashi Y, Kawahara K. Roles of vasopressin and hypertonicity in basolateral Na/K/2Cl cotransporter expression in rat kidney inner medullary collecting duct cells. Jpn J Physiol. 1999;49:201-6 pubmed
    ..However, only hypertonicity was effective in increasing the expression of rNKCC1 protein. ..
  8. Su G, Kintner D, Sun D. Contribution of Na(+)-K(+)-Cl(-) cotransporter to high-[K(+)](o)- induced swelling and EAA release in astrocytes. Am J Physiol Cell Physiol. 2002;282:C1136-46 pubmed
    ..extracellular K(+) concentration ([K(+)](o))-mediated stimulation of Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) may result in a net gain of K(+) and Cl(-) and thus lead to high-[K(+)](o)-induced swelling and glutamate release...
  9. Geng Y, Hoke A, Delpire E. The Ste20 kinases Ste20-related proline-alanine-rich kinase and oxidative-stress response 1 regulate NKCC1 function in sensory neurons. J Biol Chem. 2009;284:14020-8 pubmed publisher
    b>NKCC1 is highly expressed in dorsal root ganglion neurons, where it is involved in gating sensory information...
  10. Takiguchi Y, Sun G, Ogawa K, Matsunaga T. Long-lasting changes in the cochlear K+ recycling structures after acute energy failure. Neurosci Res. 2013;77:33-41 pubmed publisher
    ..of fibrocytes was observed in the basal turn, and the levels of the Na,K-ATPase alpha and beta1 subunits and of NKCC1 were decreased in these cells, whereas connexin 26 (Cx26) level increased in the type 1 fibrocytes adjacent to the ..
  11. Achilles K, Okabe A, Ikeda M, Shimizu Okabe C, Yamada J, Fukuda A, et al. Kinetic properties of Cl uptake mediated by Na+-dependent K+-2Cl cotransport in immature rat neocortical neurons. J Neurosci. 2007;27:8616-27 pubmed
    ..distribution by bumetanide or Na+-free solutions, suggesting a participation of Na+-K+-2Cl- cotransport isoform 1 (NKCC1) in maintaining elevated [Cl-]i. Expression of NKCC1 was found in CR cells on the mRNA and protein levels...
  12. Garg P, Martin C, Elms S, Gordon F, Wall S, Garland C, et al. Effect of the Na-K-2Cl cotransporter NKCC1 on systemic blood pressure and smooth muscle tone. Am J Physiol Heart Circ Physiol. 2007;292:H2100-5 pubmed
    Studies in rat aorta have shown that the Na-K-2Cl cotransporter NKCC1 is activated by vasoconstrictors and inhibited by nitrovasodilators, contributes to smooth muscle tone in vitro, and is upregulated in hypertension...
  13. O Keefe T, Williams G, Davies S, Neuberger M. Hyperresponsive B cells in CD22-deficient mice. Science. 1996;274:798-801 pubmed
    ..Thus, CD22 is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. ..
  14. Jiang G, Akar F, Cobbs S, Lomashvilli K, Lakkis R, Gordon F, et al. Blood pressure regulates the activity and function of the Na-K-2Cl cotransporter in vascular smooth muscle. Am J Physiol Heart Circ Physiol. 2004;286:H1552-7 pubmed
    The Na-K-2Cl cotransporter (NKCC1) is one of several transporters that have been linked to hypertension, and its inhibition reduces vascular smooth muscle tone and blood pressure...
  15. Hasbargen T, Ahmed M, Miranpuri G, Li L, Kahle K, Resnick D, et al. Role of NKCC1 and KCC2 in the development of chronic neuropathic pain following spinal cord injury. Ann N Y Acad Sci. 2010;1198:168-72 pubmed publisher
    ..cotransporters (CCCs) in the SLC12 family: the inwardly directed Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) and outwardly directed K(+)-Cl(-) cotransporter isoform 2 (KCC2)...
  16. Price T, Hargreaves K, Cervero F. Protein expression and mRNA cellular distribution of the NKCC1 cotransporter in the dorsal root and trigeminal ganglia of the rat. Brain Res. 2006;1112:146-58 pubmed
    ..The molecular basis for this GABAergic response appears to be the Na+K+2Cl- cotransporter NKCC1 that contributes to the maintenance of a high intracellular chloride concentration...
  17. Chee K, Vorontsova I, Lim J, Kistler J, Donaldson P. Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency. Mol Vis. 2010;16:800-12 pubmed
    ..To characterize the expression patterns of the Na+-K+-Cl(-) cotransporter (NKCC) 1 and NKCC2, and the Na+-Cl(-) cotransporter (NCC) in the rat lens and to determine if they play a role in regulating lens volume and transparency...
  18. Liu Q, Wong Riley M. Postnatal development of Na(+)-K(+)-2Cl(-) co-transporter 1 and K(+)-Cl(-) co-transporter 2 immunoreactivity in multiple brain stem respiratory nuclei of the rat. Neuroscience. 2012;210:1-20 pubmed publisher
    ..Since Na(+)-K(+)-2Cl(-) co-transporter 1 (NKCC1) and K(+)-Cl(-) co-transporter 2 (KCC2) play pivotal roles in determining the responses of GABA(A) and glycine ..
  19. Cramer S, Baggott C, Cain J, Tilghman J, Allcock B, Miranpuri G, et al. The role of cation-dependent chloride transporters in neuropathic pain following spinal cord injury. Mol Pain. 2008;4:36 pubmed publisher
    ..This study investigated the role of two major intracellular Cl- regulatory proteins, Na+-K+-Cl- cotransporter 1 (NKCC1) and K+-Cl- cotransporter 2 (KCC2), in neuropathic pain following spinal cord injury (SCI)...
  20. Ye Z, Li D, Byun H, Li L, Pan H. NKCC1 upregulation disrupts chloride homeostasis in the hypothalamus and increases neuronal activity-sympathetic drive in hypertension. J Neurosci. 2012;32:8560-8 pubmed publisher
    ..Na(+)-K(+)-2Cl(-) cotransporter-1 (NKCC1) in the brain is critically involved in maintaining chloride homeostasis and in neuronal responses mediated by ..
  21. Chen S, Zhu L, Chen H, Wen L, Laumet G, Pan H. Increased spinal cord Na⁺-K⁺-2Cl⁻ cotransporter-1 (NKCC1) activity contributes to impairment of synaptic inhibition in paclitaxel-induced neuropathic pain. J Biol Chem. 2014;289:31111-20 pubmed publisher
    ..Cation-chloride cotransporters, such as Na(+)-K(+)-2Cl(-) cotransporter-1 (NKCC1) and K(+)-Cl(-) cotransporter-2 (KCC2), critically influence spinal synaptic inhibition by regulating ..
  22. Moore Hoon M, Turner R. Molecular and topological characterization of the rat parotid Na+-K+-2Cl- cotransporter1. Biochim Biophys Acta. 1998;1373:261-9 pubmed
    ..The predicted amino acid sequence of this protein is highly homologous to a previously cloned NKCC1 from the shark rectal gland and to mammalian NKCC1s cloned from several cultured cell lines, confirming the ..
  23. Toyoda H, Yamada J, Ueno S, Okabe A, Kato H, Sato K, et al. Differential functional expression of cation-Cl- cotransporter mRNAs (KCC1, KCC2, and NKCC1) in rat trigeminal nervous system. Brain Res Mol Brain Res. 2005;133:12-8 pubmed
    ..nuclei, we investigated the expressions of K+-Cl- cotransporters (KCC1 and KCC2) and Na+-K+-2Cl- cotransporter (NKCC1) mRNAs by using in situ hybridization histochemistry...
  24. Miyoshi K, Meyer B, Gruss P, Cui Y, Renou J, Morgan F, et al. Mammary epithelial cells are not able to undergo pregnancy-dependent differentiation in the absence of the helix-loop-helix inhibitor Id2. Mol Endocrinol. 2002;16:2892-901 pubmed
    ..Nuclear localization of Stat5a and down-regulation of NKCC1 was observed in some areas, indicating a limited response to pregnancy signals...
  25. Jayakumar A, Valdes V, Norenberg M. The Na-K-Cl cotransporter in the brain edema of acute liver failure. J Hepatol. 2011;54:272-8 pubmed publisher
    ..found that ammonia, a key etiological factor in ALF, caused the activation of the Na-K-Cl cotransporter-1 (NKCC1) in cultured astrocytes, and that inhibition of such activation led to a reduction in astrocyte swelling, ..
  26. Chen H, Kintner D, Jones M, Matsuda T, Baba A, Kiedrowski L, et al. AMPA-mediated excitotoxicity in oligodendrocytes: role for Na(+)-K(+)-Cl(-) co-transport and reversal of Na(+)/Ca(2+) exchanger. J Neurochem. 2007;102:1783-1795 pubmed publisher
    We investigated the role of Na(+)-K(+)-Cl(-) co-transporter isoform 1 (NKCC1) and reversal of Na(+)/Ca(2+) exchanger (NCX(rev)) in glutamate-mediated excitotoxicity in oligodendrocytes obtained from rat spinal cords (postnatal day 6-8)...
  27. Diykov D, Turchinovich A, Zoidl G, Hoffmann K. Elevated intracellular chloride level in albino visual cortex neurons is mediated by Na-K-Cl co-transporter. BMC Neurosci. 2008;9:57 pubmed publisher
    ..depolarizing to a hyperpolarizing action of GABA is a consequence of a decrease of the Na+-K+-2Cl- co-transporter (NKCC1, Cl--uptake) and increase of the K+-Cl- co-transporter (KCC2, Cl--extrusion) expression...
  28. González M. Regulation of the cell surface expression of chloride transporters during epileptogenesis. Neurosci Lett. 2016;628:213-8 pubmed publisher
  29. Nakajima K, Miyazaki H, Niisato N, Marunaka Y. Essential role of NKCC1 in NGF-induced neurite outgrowth. Biochem Biophys Res Commun. 2007;359:604-10 pubmed
    ..In the present study, we report the role of NKCC1 (an isoform of NKCC) in NGF-induced neurite outgrowth of rat pheochromocytoma PC12D cells...
  30. Alahmari K, Prabhakaran H, Prabhakaran K, Chandramoorthy H, Ramugounder R. Antioxidants and NOS inhibitors selectively targets manganese-induced cell volume via Na-K-Cl cotransporter-1 in astrocytes. Brain Res. 2015;1610:69-79 pubmed publisher
    ..current study, we used rat primary astrocyte cultures to investigate the activation of Na-K-Cl cotransporter-1 (NKCC1) a downstream mechanism for free radical induced astrocyte swelling as a result of manganese toxicity...
  31. Cho H, Lee H, Kim H, Han H, Kim I. Expression of Na+-K+ -2Cl- cotransporter 1 is epigenetically regulated during postnatal development of hypertension. Am J Hypertens. 2011;24:1286-93 pubmed publisher
    The expression of Na(+)-K(+)-2Cl(-) cotransporter 1 (NKCC1) is upregulated in spontaneously hypertensive rat (SHR). We investigated whether expression of NKCC1 is epigenetically regulated during postnatal development of hypertension...
  32. Wang H, Yan Y, Kintner D, Lytle C, Sun D. GABA-mediated trophic effect on oligodendrocytes requires Na-K-2Cl cotransport activity. J Neurophysiol. 2003;90:1257-65 pubmed
    The Na-K-2Cl cotransporter isoform1 (NKCC1) is present in many animal cells where it plays prominent roles in regulating cell volume and maintaining intracellular Cl(-) concentration ([Cl(-)]i) above electrochemical equilibrium...
  33. Worrell R, Oghene J, Matthews J. Ammonium effects on colonic Cl- secretion: anomalous mole fraction behavior. Am J Physiol Gastrointest Liver Physiol. 2004;286:G14-22 pubmed
    ..The overall effect of basolateral NH4+ on cAMP-stimulated transport is dependent on the [K+]o /[NH4+]o ratio at the basolateral membrane, where o is outside of the cell. ..
  34. Murguía Castillo J, Beas Zarate C, Rivera Cervantes M, Feria Velasco A, Ureña Guerrero M. NKCC1 and KCC2 protein expression is sexually dimorphic in the hippocampus and entorhinal cortex of neonatal rats. Neurosci Lett. 2013;552:52-7 pubmed publisher
    ..GABA-mediated excitation has been observed when the NKCC1 (chloride importer) expression level is higher than KCC2 (chloride exporter)...
  35. Shimizu Okabe C, Okabe A, Kilb W, Sato K, Luhmann H, Fukuda A. Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Neurosci Res. 2007;59:288-95 pubmed
    ..We demonstrated, by in situ hybridization histochemistry for cation-Cl(-) cotrtansporters, that NKCC1 mRNA expression was upregulated and KCC2 mRNA expression downregulated at P4 in "bridge" structure (..
  36. Dehaye J, Nagy A, Premkumar A, Turner R. Identification of a functionally important conformation-sensitive region of the secretory Na+-K+-2Cl- cotransporter (NKCC1). J Biol Chem. 2003;278:11811-7 pubmed
    The secretory Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) is a member of a small gene family of electroneutral salt transporters that play essential roles in salt and water homeostasis in many mammalian tissues...
  37. Tsuchiya Y, Nakashima S, Banno Y, Suzuki Y, Morita H. Effect of high-NaCl or high-KCl diet on hepatic Na+- and K+-receptor sensitivity and NKCC1 expression in rats. Am J Physiol Regul Integr Comp Physiol. 2004;286:R591-6 pubmed
    We previously reported that the bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter (NKCC1) is involved in the hepatic Na+ and K+ sensor mechanism...
  38. Schliess F, Schafer C, vom Dahl S, Fischer R, Lordnejad M, Haussinger D. Expression and regulation of the Na(+)/K(+)/2Cl(-) cotransporter NKCC1 in rat liver and human HuH-7 hepatoma cells. Arch Biochem Biophys. 2002;401:187-97 pubmed
    The expression of sodium potassium chloride cotransporter 1 (NKCC1) was studied in different liver cell types. NKCC1 was found in rat liver parenchymal and sinusoidal endothelial cells and in human HuH-7 hepatoma cells...
  39. Perrot Sinal T, Sinal C, Reader J, Speert D, McCarthy M. Sex differences in the chloride cotransporters, NKCC1 and KCC2, in the developing hypothalamus. J Neuroendocrinol. 2007;19:302-8 pubmed
    ..Here, we investigate the expression of the chloride cotransporters, NKCC1 (Na-K-2Cl(-)) and KCC2 (K-Cl(-)) in neonate mediobasal hypothalamus of male and female rats...
  40. Kaneko H, Putzier I, Frings S, Kaupp U, Gensch T. Chloride accumulation in mammalian olfactory sensory neurons. J Neurosci. 2004;24:7931-8 pubmed
    ..Functional assays and PCR experiments revealed that NKCC1-mediated Cl(-) uptake through the apical membrane counteracts Cl(-) depletion in the sensory cilia, and thus ..
  41. Marty S, Wehrle R, Alvarez Leefmans F, Gasnier B, Sotelo C. Postnatal maturation of Na+, K+, 2Cl- cotransporter expression and inhibitory synaptogenesis in the rat hippocampus: an immunocytochemical analysis. Eur J Neurosci. 2002;15:233-45 pubmed
  42. Côté M, Gandhi S, Zambrotta M, Houle J. Exercise modulates chloride homeostasis after spinal cord injury. J Neurosci. 2014;34:8976-87 pubmed publisher
    ..which is determined largely by the expression of two cation-chloride cotransporters (CCCs), KCC2 and NKCC1, which serve as chloride exporters and importers, respectively...
  43. Wang C, Shimizu Okabe C, Watanabe K, Okabe A, Matsuzaki H, Ogawa T, et al. Developmental changes in KCC1, KCC2, and NKCC1 mRNA expressions in the rat brain. Brain Res Dev Brain Res. 2002;139:59-66 pubmed
    We investigated the expressions of KCC1, KCC2 and NKCC1 mRNAs in the developing rat brain. The neuroepithelium showed abundant KCC1 and NKCC1 mRNA expressions, while KCC2 mRNA was not detected there...
  44. Pitcher M, Cervero F. Role of the NKCC1 co-transporter in sensitization of spinal nociceptive neurons. Pain. 2010;151:756-62 pubmed publisher
    The Na(+), K(+), 2Cl(-) co-transporter type 1 (NKCC1) plays a pivotal role in hyperalgesia associated with inflammatory stimuli...
  45. Santos L, da Silveira G, Costa V, Batista A, Madureira A, Rodrigues A, et al. Alcohol abuse promotes changes in non-synaptic epileptiform activity with concomitant expression changes in cotransporters and glial cells. PLoS ONE. 2013;8:e78854 pubmed publisher
    ..The DG regions were analyzed for the presence of NKCC1, KCC2, GFAP and CD11b immunoreactivity and cell density...
  46. Vitari A, Thastrup J, Rafiqi F, Deak M, Morrice N, Karlsson H, et al. Functional interactions of the SPAK/OSR1 kinases with their upstream activator WNK1 and downstream substrate NKCC1. Biochem J. 2006;397:223-31 pubmed
    ..proline/alanine-rich kinase) and OSR1 (oxidative stress-responsive kinase-1) kinases interact and phosphorylate NKCC1 (Na+-K+-2Cl- co-transporter-1), leading to its activation...
  47. Kurihara K, Nakanishi N, Moore Hoon M, Turner R. Phosphorylation of the salivary Na(+)-K(+)-2Cl(-) cotransporter. Am J Physiol Cell Physiol. 2002;282:C817-23 pubmed
    We studied the phosphorylation of the secretory Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) in rat parotid acinar cells...
  48. Baroncelli L, Cenni M, Melani R, Deidda G, Landi S, Narducci R, et al. Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals. Neuropharmacology. 2017;113:167-177 pubmed publisher
    ..Early IGF-1 decreased the ratio between the expression of NKCC1 and KCC2 cation/chloride transporters, and the reversal potential for GABAAR-driven Cl- ..
  49. Gao Y, Zhou J, Zhu Y, Kosten T, Li D. Chronic Unpredictable Mild Stress Induces Loss of GABA Inhibition in Corticotrophin-Releasing Hormone-Expressing Neurons through NKCC1 Upregulation. Neuroendocrinology. 2017;104:194-208 pubmed publisher
    ..CUMS induced a long-lasting increase in expression levels of the cation chloride cotransporter Na+-K+-Cl--Cl- (NKCC1) in the PVN but a transient decrease in expression levels of K+-Cl--Cl- in the PVN, which returned to the basal ..
  50. Wang X, Breaks J, Loutzenhiser K, Loutzenhiser R. Effects of inhibition of the Na+/K+/2Cl- cotransporter on myogenic and angiotensin II responses of the rat afferent arteriole. Am J Physiol Renal Physiol. 2007;292:F999-F1006 pubmed
    ..Bumetanide also inhibited ANG II-induced afferent arteriolar vasconstriction at similar concentrations. Finally, NKCC1, but not NKCC2, expression was demonstrated in the afferent arteriole by RT-PCR and the presence of NKCC1 in ..
  51. Kim J, Jung Y. Increased aquaporin-1 and Na+ -K+ -2Cl- cotransporter 1 expression in choroid plexus leads to blood-cerebrospinal fluid barrier disruption and necrosis of hippocampal CA1 cells in acute rat models of hyponatremia. J Neurosci Res. 2012;90:1437-44 pubmed publisher
    ..of choroid plexus aquaporin-1 (AQP1) and of cation chloride transporters (Na(+) -K(+) -2Cl(-) cotransporter 1 [NKCC1] and K(+) -Cl(-) cotransporter 4 [KCC4]) in the underlying hippocampal pathophysiology of hyponatremia in acute (6 ..
  52. Kao L, Kurtz L, Shao X, Papadopoulos M, Liu L, Bok D, et al. Severe neurologic impairment in mice with targeted disruption of the electrogenic sodium bicarbonate cotransporter NBCe2 (Slc4a5 gene). J Biol Chem. 2011;286:32563-74 pubmed publisher
    ..Our findings are the first demonstration of the fundamental importance of NBCe2 in the biology of the nervous system. ..
  53. Jayakumar A, Liu M, Moriyama M, Ramakrishnan R, Forbush B, Reddy P, et al. Na-K-Cl Cotransporter-1 in the mechanism of ammonia-induced astrocyte swelling. J Biol Chem. 2008;283:33874-82 pubmed publisher
    ..In particular, activation of the Na-K-Cl cotransporter (NKCC1) has been shown to be involved in cell swelling in several neurological disorders...
  54. Nugent B, Valenzuela C, Simons T, McCarthy M. Kinases SPAK and OSR1 are upregulated by estradiol and activate NKCC1 in the developing hypothalamus. J Neurosci. 2012;32:593-8 pubmed publisher
    ..The driving force for Cl(-) is outward because the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1) elevates the Cl(-) concentration in these cells...
  55. Hunt D, Yamoah E, Krubitzer L. Multisensory plasticity in congenitally deaf mice: how are cortical areas functionally specified?. Neuroscience. 2006;139:1507-24 pubmed
  56. Ostroumov A, Simonetti M, Nistri A. Cystic fibrosis transmembrane conductance regulator modulates synaptic chloride homeostasis in motoneurons of the rat spinal cord during neonatal development. Dev Neurobiol. 2011;71:253-68 pubmed publisher
    ..The gene activities of the Cl(-) inward transporter NKCC1 and CFTR were positively correlated and grew between P1 and P8...
  57. Orlov S. NKCC1 as a regulator of vascular tone and a novel target for antihypertensive therapeutics. Am J Physiol Heart Circ Physiol. 2007;292:H2035-6 pubmed
  58. Balena T, Woodin M. Coincident pre- and postsynaptic activity downregulates NKCC1 to hyperpolarize E(Cl) during development. Eur J Neurosci. 2008;27:2402-12 pubmed publisher
    ..coincident pre- and postsynaptic activity can also modulate immature GABAergic synapses, where the Na+-K+-2Cl- (NKCC1) cotransporter maintains a relatively high level of intracellular chloride ([Cl-](i))...
  59. Fu P, Tang R, Yu Z, Huang S, Xie M, Luo X, et al. Bumetanide-induced NKCC1 inhibition attenuates oxygen-glucose deprivation-induced decrease in proliferative activity and cell cycle progression arrest in cultured OPCs via p-38 MAPKs. Brain Res. 2015;1613:110-9 pubmed publisher
    The Na-K-Cl co-transporter 1 (NKCC1; a member of the cation-chloride co-transporter family) mediates the coupled movement of Na(+) and/or K(+) with Cl(-) across the plasma membrane of cells (Haas and Forbush, 2000, Annu. Rev. Physiol...
  60. Yan Y, Dempsey R, Flemmer A, Forbush B, Sun D. Inhibition of Na(+)-K(+)-Cl(-) cotransporter during focal cerebral ischemia decreases edema and neuronal damage. Brain Res. 2003;961:22-31 pubmed
    Our previous study demonstrated that pharmacological inhibition of the Na(+)-K(+)-Cl(-) cotransporter isoform 1 (NKCC1) during ischemia and reperfusion attenuated neuronal damage and edema...
  61. Worrell R, Merk L, Matthews J. Ammonium transport in the colonic crypt cell line, T84: role for Rhesus glycoproteins and NKCC1. Am J Physiol Gastrointest Liver Physiol. 2008;294:G429-40 pubmed
    ..NH(4)(+) transport in the secretory direction was supported by basolateral NH(4)(+) loading on NKCC1, Na(+)-K(+)-ATPase, and the NH(4)(+) transporter, RhBG...
  62. Chou C, Yu M, Kassai E, Morris R, Hoffert J, Wall S, et al. Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct. Am J Physiol Renal Physiol. 2008;295:F192-201 pubmed publisher
    ..AVP-induced cell swelling was absent in IMCDs from NKCC1-knockout mice...
  63. Santos L, Rodrigues A, Lopes M, Costa V, Scorza C, Scorza F, et al. Long-term alcohol exposure elicits hippocampal nonsynaptic epileptiform activity changes associated with expression and functional changes in NKCC1, KCC2 co-transporters and Na+/K+-ATPase. Neuroscience. 2017;340:530-541 pubmed publisher
    Nonsynaptic mechanism changes, particularly the enhancement of NKCC1 expression in the dentate gyrus (DG) after 4weeks of ethanol consumption, motivate the present work, in which rats were submitted to a period of chronic consumption (..
  64. Lu K, Huang T, Wang J, You Y, Chou J, Chan M, et al. NKCC1 mediates traumatic brain injury-induced hippocampal neurogenesis through CREB phosphorylation and HIF-1α expression. Pflugers Arch. 2015;467:1651-61 pubmed publisher
    ..We previously had evidenced that TBI induced Na-K-2Cl co-transporter (NKCC1) upregulation in hippocampus...
  65. Zhao H, Hyde R, Hundal H. Signalling mechanisms underlying the rapid and additive stimulation of NKCC activity by insulin and hypertonicity in rat L6 skeletal muscle cells. J Physiol. 2004;560:123-36 pubmed
    ..Furthermore, activation of NKCC in response to hyperosmotic-induced cell shrinkage represents a critical component of the RVI mechanism that allows L6 muscle cells to volume regulate. ..
  66. Dowd B, Forbush B. PASK (proline-alanine-rich STE20-related kinase), a regulatory kinase of the Na-K-Cl cotransporter (NKCC1). J Biol Chem. 2003;278:27347-53 pubmed
    Although the phosphorylation-dependent activation of the Na-K-Cl cotransporter (NKCC1) has been previously well documented, the identity of the kinase(s) responsible for this regulation has proven elusive. Recently, Piechotta et al...
  67. Valero M, Garay R, Gros P, Alda J. Cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel and Na-K-Cl cotransporter NKCC1 isoform mediate the vasorelaxant action of genistein in isolated rat aorta. Eur J Pharmacol. 2006;544:126-31 pubmed
    ..fibrosis transmembrane conductance regulator (CFTR) chloride channel, and its associated Na-K-Cl cotransporter NKCC1. Isolated, endothelium-denuded rat aorta was contracted with phenylephrine 1 microM, and the vasorelaxant ..
  68. Solymosi E, Kaestle Gembardt S, Vadász I, Wang L, Neye N, Chupin C, et al. Chloride transport-driven alveolar fluid secretion is a major contributor to cardiogenic lung edema. Proc Natl Acad Sci U S A. 2013;110:E2308-16 pubmed publisher
    ..Our results characterize an innovative mechanism of cardiogenic edema formation and identify NKCC1 as a unique therapeutic target in cardiogenic lung edema.