Gene Symbol: SHARP 2
Description: basic helix-loop-helix family, member e40
Alias: Bhlhb2, Dec1, SHARP-2, Sharp2, Stra13, Stra14, class E basic helix-loop-helix protein 40, basic helix-loop-helix domain containing, class B2, class B basic helix-loop-helix protein 2, enhancer-of-split and hairy-related protein 2, stimulated by retinoic acid 14
Species: rat
Products:     SHARP 2

Top Publications

  1. Honma S, Kawamoto T, Takagi Y, Fujimoto K, Sato F, Noshiro M, et al. Dec1 and Dec2 are regulators of the mammalian molecular clock. Nature. 2002;419:841-4 pubmed
    ..We found that Dec1 and Dec2, basic helix-loop-helix transcription factors, repressed Clock/Bmal1-induced transactivation of the mouse ..
  2. Hirano S, Yamada K, Kawata H, Shou Z, Mizutani T, Shigematsu Y, et al. The rat enhancer of split- and hairy-related protein-2 gene: hepatic expression, genomic structure, and promoter analysis. Arch Biochem Biophys. 2004;422:81-90 pubmed
    ..Therefore, other E box-binding transcription factors differing from USF proteins or USF-associated proteins are necessary for transcriptional stimulation of the rat SHARP-2 gene. ..
  3. Sato F, Kawamoto T, Fujimoto K, Noshiro M, Honda K, Honma S, et al. Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation. Interaction with BMAL1. Eur J Biochem. 2004;271:4409-19 pubmed
    The basic helix-loop-helix transcription factor DEC1 is expressed in a circadian manner in the suprachiasmatic nucleus where it seems to play a role in regulating the mammalian circadian rhythm by suppressing the CLOCK/BMAL1-activated ..
  4. Wu T, Ni Y, Kato H, Fu Z. Feeding-induced rapid resetting of the hepatic circadian clock is associated with acute induction of Per2 and Dec1 transcription in rats. Chronobiol Int. 2010;27:1-18 pubmed publisher
    ..A 30 min feeding stimulus was sufficient to significantly induce the expression of Per2 and Dec1 within 1 h and alter the transcript levels and circadian phases of other selected clock genes (Bmal1, Cry1, Per1, ..
  5. Fujimoto K, Hamaguchi H, Hashiba T, Nakamura T, Kawamoto T, Sato F, et al. Transcriptional repression by the basic helix-loop-helix protein Dec2: multiple mechanisms through E-box elements. Int J Mol Med. 2007;19:925-32 pubmed
    ..Gel retardation and luciferase assays showed that Dec2, as well as its related protein Dec1, preferentially binds to class B E-box elements (CACGTG) as a homodimer and represses the transcription of target ..
  6. Yamada K, Ogata Kawata H, Matsuura K, Miyamoto K. SHARP-2/Stra13/DEC1 as a potential repressor of phosphoenolpyruvate carboxykinase gene expression. FEBS Lett. 2005;579:1509-14 pubmed
    ..Based on these findings, we conclude that SHARP-2 is a potential repressor of PEPCK gene expression. ..
  7. Yamada K, Kawata H, Mizutani T, Arima T, Yazawa T, Matsuura K, et al. Gene expression of basic helix-loop-helix transcription factor, SHARP-2, is regulated by gonadotropins in the rat ovary and MA-10 cells. Biol Reprod. 2004;70:76-82 pubmed
    ..We conclude that the level of SHARP-2 mRNA is regulated by gonadotropins and that SHARP-2 functions as a transcriptional repressor localized in the nucleus. ..
  8. Kawamoto T, Noshiro M, Sato F, Maemura K, Takeda N, Nagai R, et al. A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation. Biochem Biophys Res Commun. 2004;313:117-24 pubmed
    ..We show here an autofeedback loop of Dec1 encoding a basic helix-loop-helix transcription factor: CLOCK/BMAL increased the promoter activity of Dec1, and ..
  9. St Pierre B, Flock G, Zacksenhaus E, Egan S. Stra13 homodimers repress transcription through class B E-box elements. J Biol Chem. 2002;277:46544-51 pubmed
    A mammalian basic helix-loop-helix protein known variably as Stra13, Sharp2, and Dec1 has been implicated in cell activation, proliferation, and differentiation...

More Information


  1. Iizuka K, Horikawa Y. Regulation of lipogenesis via BHLHB2/DEC1 and ChREBP feedback looping. Biochem Biophys Res Commun. 2008;374:95-100 pubmed publisher
    b>BHLHB2/DEC1 is a transcription factor implicated in cell proliferation, apoptosis, and metabolism, and is also known to play an important role in the regulation of the mammalian circadian rhythm...
  2. Hamaguchi H, Fujimoto K, Kawamoto T, Noshiro M, Maemura K, Takeda N, et al. Expression of the gene for Dec2, a basic helix-loop-helix transcription factor, is regulated by a molecular clock system. Biochem J. 2004;382:43-50 pubmed
    ..Transcripts of Dec2, as well as those of its related gene Dec1, exhibit a striking circadian oscillation in the suprachiasmatic nucleus, and Dec2 inhibits transcription from the ..
  3. Nakashima A, Kawamoto T, Honda K, Ueshima T, Noshiro M, Iwata T, et al. DEC1 modulates the circadian phase of clock gene expression. Mol Cell Biol. 2008;28:4080-92 pubmed publisher
    b>DEC1 suppresses CLOCK/BMAL1-enhanced promoter activity, but its role in the circadian system of mammals remains unclear...
  4. Kon N, Hirota T, Kawamoto T, Kato Y, Tsubota T, Fukada Y. Activation of TGF-beta/activin signalling resets the circadian clock through rapid induction of Dec1 transcripts. Nat Cell Biol. 2008;10:1463-9 pubmed publisher
    ..The resetting was mediated by an immediate-early induction of Dec1, a gene whose physiological role in the function of the circadian clock has been unclear...
  5. Rossner M, Dorr J, Gass P, Schwab M, Nave K. SHARPs: mammalian enhancer-of-split- and hairy-related proteins coupled to neuronal stimulation. Mol Cell Neurosci. 1997;10:460-75 pubmed
    ..This suggests that neuronal bHLH proteins are also involved in the "adaptive" changes of mature CNS neurons which are coupled to glutamatergic stimulation. ..
  6. Wu T, Ni Y, Zhuge F, Sun L, Xu B, Kato H, et al. Significant dissociation of expression patterns of the basic helix-loop-helix transcription factors Dec1 and Dec2 in rat kidney. J Exp Biol. 2011;214:1257-63 pubmed publisher
    b>Dec1 and Dec2 are regulators of the mammalian molecular clock that show robust circadian rhythms in the suprachiasmatic nucleus and various peripheral tissues...
  7. Komatsu Y, Yanagisawa Y, Moriizumi M, Tsuchiya Y, Yokouchi H, Otsuka H, et al. 5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside stimulates the rat enhancer of split- and hairy-related protein-2 gene via atypical protein kinase C lambda. J Biochem. 2016;159:429-36 pubmed publisher
    ..Thus, we conclude that the AICAR-induction of theSHARP-2gene is mediated at transcription level by a PI 3-K/aPKCλ pathway. ..
  8. Butler M, Honma S, Fukumoto T, Kawamoto T, Fujimoto K, Noshiro M, et al. Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice. J Biol Rhythms. 2004;19:126-34 pubmed
    b>DEC1 and DEC2 are basic helix-loop-helix transcription factors that functionally resemble negative feedback components of the mammalian circadian clock...
  9. Li Y, Xie M, Song X, Gragen S, Sachdeva K, Wan Y, et al. DEC1 negatively regulates the expression of DEC2 through binding to the E-box in the proximal promoter. J Biol Chem. 2003;278:16899-907 pubmed
    ..Recently, we have reported that DEC1 is abundantly expressed in colon carcinomas but not in the adjacent normal tissues...
  10. Wu L, Cheng W, Yu M, He B, Sun H, Chen Q, et al. Nr3C1-Bhlhb2 Axis Dysregulation Is Involved in the Development of Attention Deficit Hyperactivity. Mol Neurobiol. 2017;54:1196-1212 pubmed publisher
    ..indicated that all miR-138, 138*, 34c*, 296, and 494 targeted the 3' untranslated region of transcription factor Bhlhb2 (Bhlhe40) messenger RNA (mRNA) in common and ectopic expression of miR-138,138*, 34c*, 296, and 494 further ..
  11. Kanai Y, Asano K, Komatsu Y, Takagi K, Ono M, Tanaka T, et al. Induction of the SHARP-2 mRNA level by insulin is mediated by multiple signaling pathways. Biosci Biotechnol Biochem. 2017;81:256-261 pubmed publisher
    ..Thus, we conclude that insulin induces the expression of the rat SHARP-2 gene at the transcription level via both a PI 3-K/aPKC?- and a PI 3-K/mTOR- pathways and that protein synthesis is required for this induction. ..
  12. Li Y, Song X, Ma Y, Liu J, Yang D, Yan B. DNA binding, but not interaction with Bmal1, is responsible for DEC1-mediated transcription regulation of the circadian gene mPer1. Biochem J. 2004;382:895-904 pubmed
    b>DEC1 (differentially expressed in chondrocytes 1) and DEC2 are E-box-binding transcription factors and exhibit a circadian expression pattern. Recently, both proteins were found to repress the Clock/Bmal1-activated E-box promoters (e.g...
  13. Yamada K, Kawata H, Shou Z, Mizutani T, Noguchi T, Miyamoto K. Insulin induces the expression of the SHARP-2/Stra13/DEC1 gene via a phosphoinositide 3-kinase pathway. J Biol Chem. 2003;278:30719-24 pubmed
    ..In addition, nuclear run-on assay revealed that transcription of the rat SHARP-2 gene was induced by insulin. Thus, we conclude that insulin induces the transcription of the rat SHARP-2 gene via a phosphoinositide 3-kinase pathway. ..
  14. Cho Y, Noshiro M, Choi M, Morita K, Kawamoto T, Fujimoto K, et al. The basic helix-loop-helix proteins differentiated embryo chondrocyte (DEC) 1 and DEC2 function as corepressors of retinoid X receptors. Mol Pharmacol. 2009;76:1360-9 pubmed publisher
    The basic helix-loop-helix proteins differentiated embryo chondrocyte 1 (DEC1) and DEC2 are involved in circadian rhythm control...