Gene Symbol: Scp2
Description: sterol carrier protein 2
Alias: NSLIPTR, SCPx, non-specific lipid-transfer protein, NSL-TP, SCP-2, SCP-X, SCP-chi, Sterol carrier protein 2, liver, propanoyl-CoA C-acyltransferase, sterol carrier protein X
Species: rat
Products:     Scp2

Top Publications

  1. Ren S, Hylemon P, Marques D, Hall E, Redford K, Gil G, et al. Effect of increasing the expression of cholesterol transporters (StAR, MLN64, and SCP-2) on bile acid synthesis. J Lipid Res. 2004;45:2123-31 pubmed
    ..These results suggest that MLN64, in its full-length form, is not responsible for the transport of cholesterol to the mitochondria or the endoplasmic reticulum, where CYP27A1 or CYP7A1 is located, respectively. ..
  2. Stanley W, Versluis K, Schultz C, Heck A, Wilmanns M. Investigation of the ligand spectrum of human sterol carrier protein 2 using a direct mass spectrometry assay. Arch Biochem Biophys. 2007;461:50-8 pubmed
    Sterol carrier protein 2 (SCP2) has been investigated by nearly native electrospray ionisation mass spectrometry in the presence of long chain fatty acyl CoAs (LCFA-CoAs) and carnitine derivatives of equivalent fatty acid chain length (..
  3. Mori T, Tsukamoto T, Mori H, Tashiro Y, Fujiki Y. Molecular cloning and deduced amino acid sequence of nonspecific lipid transfer protein (sterol carrier protein 2) of rat liver: a higher molecular mass (60 kDa) protein contains the primary sequence of nonspecific lipid transfer protein as its C-ter. Proc Natl Acad Sci U S A. 1991;88:4338-42 pubmed
    ..In Northern blot analysis, two major RNA bands, 0.85 and 2.2 kilobases (kb) long, were detected together with two minor bands of 1.6 and 2.9 kb. The 0.85- and 2.2-kb RNAs most likely encode the 15-and 60-kDa proteins, respectively. ..
  4. van Heusden G, Bos K, Wirtz K. The occurrence of soluble and membrane-bound non-specific lipid transfer protein (sterol carrier protein 2) in rat tissues. Biochim Biophys Acta. 1990;1046:315-21 pubmed
    ..5), indicated that nsL-TP is a periferal protein. It was shown by chromatofocussing that nsL-TP extracted from membrane fractions was more basic than nsL-TP present in the cytosol fraction from rat liver (isoelectric point of 8.7). ..
  5. Mendis Handagama S, Watkins P, Gelber S, Scallen T, Zirkin B, Ewing L. Luteinizing hormone causes rapid and transient changes in rat Leydig cell peroxisome volume and intraperoxisomal sterol carrier protein-2 content. Endocrinology. 1990;127:2947-54 pubmed
    ..effects of a single injection of LH on rat Leydig cell peroxisome volume and peroxisomal sterol carrier protein-2 (SCP2) content...
  6. Lopez D, Niesen M, Bedi M, McLean M. Lauric acid dependent enhancement in hepatic SCPx protein requires an insulin deficient environment. Prostaglandins Leukot Essent Fatty Acids. 2008;78:131-5 pubmed publisher
    b>Sterol carrier protein X (SCPx) is a peroxisomal protein with both lipid transfer and thiolase activity...
  7. Billheimer J, Strehl L, Davis G, Strauss J, Davis L. Characterization of a cDNA encoding rat sterol carrier protein2. DNA Cell Biol. 1990;9:159-65 pubmed
    Sterol carrier protein2 (SCP2) is a 13.2-kD protein that is thought to be involved in the intracellular transport of cholesterol...
  8. McLean M, Puryear T, Khan I, Azhar S, Billheimer J, Orly J, et al. Estradiol regulation of sterol carrier protein-2 independent of cytochrome P450 side-chain cleavage expression in the rat corpus luteum. Endocrinology. 1989;125:1337-44 pubmed
    ..P450 side-chain cleavage enzyme (P450scc) and the cholesterol transport protein, sterol carrier protein-2 (SCP2), was examined...
  9. Martin G, Hostetler H, McIntosh A, Tichy S, Williams B, Russell D, et al. Structure and function of the sterol carrier protein-2 N-terminal presequence. Biochemistry. 2008;47:5915-34 pubmed publisher
    ..These data indicate the importance of the N-terminal presequence in regulating SCP-2 structure, cholesterol localization within the ligand binding site, membrane association, and, potentially, intracellular targeting. ..

More Information


  1. Heikoop J, Ossendorp B, Wanders R, Wirtz K, Tager J. Subcellular localisation and processing of non-specific lipid transfer protein are not aberrant in Rhizomelic Chondrodysplasia Punctata fibroblasts. FEBS Lett. 1992;299:201-4 pubmed
    ..Thus the component of the import machinery defective in RCDP is not required for the import of nsLTP into peroxisomes. ..
  2. Antonenkov V, Van Veldhoven P, Mannaerts G. Isolation and subunit composition of native sterol carrier protein 2/3-oxoacyl-coenzyme A thiolase from normal rat liver peroxisomes. Protein Expr Purif. 2000;18:249-56 pubmed
  3. Seedorf U, Brysch P, Engel T, Schrage K, Assmann G. Sterol carrier protein X is peroxisomal 3-oxoacyl coenzyme A thiolase with intrinsic sterol carrier and lipid transfer activity. J Biol Chem. 1994;269:21277-83 pubmed pre-SCP2 and a 404-amino acid N-terminal domain with unknown biochemical activity or function (named sterol carrier protein x, SCPx)...
  4. McLean M, Billheimer J, Warden K, Irby R. Differential expression of hepatic sterol carrier proteins in the streptozotocin-treated diabetic rat. Endocrinology. 1995;136:3360-8 pubmed
    Sterol carrier protein-2 (SCP2) is a 13...
  5. Megli F, De Lisi A, van Amerongen A, Wirtz K, Quagliariello E. Nonspecific lipid transfer protein (sterol carrier protein 2) is bound to rat liver mitochondria: its role in spontaneous intermembrane phospholipid transfer. Biochim Biophys Acta. 1986;861:463-70 pubmed
    ..We could estimate that up to 85% of the spontaneous phospholipid transfer between vesicles and rat liver mitochondria was mediated by this transfer protein. ..
  6. Morris H, Larsen B, Billheimer J. A mass spectrometric study of the structure of sterol carrier protein SCP2 from rat liver. Biochem Biophys Res Commun. 1988;154:476-82 pubmed
    The amino acid sequence of Sterol Carrier Protein2 (SCP2) isolated from rat has been investigated. Using a novel mass spectrometric mapping approach, the C-terminus was found to be extended beyond the previously published sequence...
  7. Schroeder F, Zhou M, Swaggerty C, Atshaves B, Petrescu A, Storey S, et al. Sterol carrier protein-2 functions in phosphatidylinositol transfer and signaling. Biochemistry. 2003;42:3189-202 pubmed
    ..Taken together, these data show for the first time that SCP-2 plays a hitherto unrecognized role in intracellular phosphatidylinositol transfer, distribution, and signaling. ..
  8. Lopez D, Irby R, McLean M. Peroxisome proliferator-activated receptor alpha induces rat sterol carrier protein x promoter activity through two peroxisome proliferator-response elements. Mol Cell Endocrinol. 2003;205:169-84 pubmed
    b>Sterol carrier protein x (SCPx) plays a critical role in the peroxisomal oxidation of fatty acids...
  9. Seedorf U, Assmann G. Cloning, expression, and nucleotide sequence of rat liver sterol carrier protein 2 cDNAs. J Biol Chem. 1991;266:630-6 pubmed
    ..most upstream suggests that this cDNA is a nearly full-length cDNA coding for a protein of 547 amino acids (SCP chi)...
  10. Keller G, Scallen T, Clarke D, Maher P, Krisans S, Singer S. Subcellular localization of sterol carrier protein-2 in rat hepatocytes: its primary localization to peroxisomes. J Cell Biol. 1989;108:1353-61 pubmed
    ..The presence of SCP-2 inside peroxisomes and mitochondria raises questions about the mechanisms involved in the differential targeting of SCP-2 to these organelles. ..
  11. Ossendorp B, van Heusden G, Wirtz K. The amino acid sequence of rat liver non-specific lipid transfer protein (sterol carrier protein 2) is present in a high molecular weight protein: evidence from cDNA analysis. Biochem Biophys Res Commun. 1990;168:631-6 pubmed
    ..The largest cDNA clone consisted of 1851 bp starting at the 5' end with an open reading frame of 1545 bp. The 369 bp located at the 3' end of this open reading frame corresponded with the amino acid sequence of nsL-TP. ..
  12. Hirai A, Kino T, Tokinaga K, Tahara K, Tamura Y, Yoshida S. Regulation of sterol carrier protein 2 (SCP2) gene expression in rat peritoneal macrophages during foam cell formation. A key role for free cholesterol content. J Clin Invest. 1994;94:2215-23 pubmed
    Sterol carrier protein 2 (SCP2) has been shown to be involved in intracellular transport and metabolism of cholesterol. However, there have been no reports concerning SCP2 in macrophages, the major source of atheromatous foam cells...
  13. Williams C, Schueller N, Thompson C, van den Berg M, van Haren S, Erdmann R, et al. The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition. BMC Biochem. 2011;12:12 pubmed publisher
    ..In this study, we have tested the contribution of a second, ancillary receptor-cargo binding site, which was found in addition to the characterised Peroxisomal Target Signal type 1...
  14. Vila A, Levchenko V, Korytowski W, Girotti A. Sterol carrier protein-2-facilitated intermembrane transfer of cholesterol- and phospholipid-derived hydroperoxides. Biochemistry. 2004;43:12592-605 pubmed
    ..On the basis of these findings, we postulate that SCP-2, by trafficking ChOOHs and PLOOHs in addition to parent lipids, might exacerbate cell injury under oxidative stress conditions. ..
  15. Ferdinandusse S, Denis S, Mooijer P, Zhang Z, Reddy J, Spector A, et al. Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid. J Lipid Res. 2001;42:1987-95 pubmed
    ..and rhizomelic chondrodysplasia punctata type 1, and of fibroblasts from l-bifunctional protein and sterol carrier protein X (SCPx) knockout mice, show that the main enzymes involved in beta-oxidation of C24:6n-3 to C22:6n-3 are ..
  16. Ossendorp B, van Heusden G, De Beer A, Bos K, Schouten G, Wirtz K. Identification of the cDNA clone which encodes the 58-kDa protein containing the amino acid sequence of rat liver non-specific lipid-transfer protein (sterol-carrier protein 2). Homology with rat peroxisomal and mitochondrial 3-oxoacyl-CoA thiolases. Eur J Biochem. 1991;201:233-9 pubmed
    ..Mutant CHO cells, deficient in peroxisomes, lack nsLTP. We have found that the mRNA encoding nsLTP is still present in these cells, which suggests that the absence of this protein is related to the lack of peroxisomes. ..
  17. Kriska T, Levchenko V, Korytowski W, Atshaves B, Schroeder F, Girotti A. Intracellular dissemination of peroxidative stress. Internalization, transport, and lethal targeting of a cholesterol hydroperoxide species by sterol carrier protein-2-overexpressing hepatoma cells. J Biol Chem. 2006;281:23643-51 pubmed
    ..This is the first reported evidence that peroxidative stress damage can be selectively targeted and exacerbated by an intracellular lipid transfer protein. ..
  18. Pastuszyn A, Noland B, Bazan J, Fletterick R, Scallen T. Primary sequence and structural analysis of sterol carrier protein 2 from rat liver: homology with immunoglobulins. J Biol Chem. 1987;262:13219-27 pubmed
    Sterol carrier protein 2 (SCP2) is involved in the later steps of cholesterol biosynthesis and in the intracellular transport of cholesterol. In the present investigation, the amino acid sequence of SCP2 from rat liver has been determined...
  19. Shiozawa K, Konarev P, Neufeld C, Wilmanns M, Svergun D. Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. J Biol Chem. 2009;284:25334-42 pubmed publisher
    ..These results suggest that the extended structure of Pex5p may play a role in interactions with other substrates such as lipids and membrane proteins during the formation of functional multiprotein complexes. ..
  20. Nishioka Y, Yamaguchi M, Kawakami A, Munehiro M, Masuda S, Tomaru U, et al. Type II Natural Killer T Cells that Recognize Sterol Carrier Protein 2 Are Implicated in Vascular Inflammation in the Rat Model of Systemic Connective Tissue Diseases. Am J Pathol. 2017;187:176-186 pubmed publisher
    ..Sequencing identified the rat cDNA introduced into 4D2 as sterol carrier protein 2 (SCP2)...
  21. Kraemer R, Pomerantz K, Kesav S, Scallen T, Hajjar D. Cholesterol enrichment enhances expression of sterol-carrier protein-2: implications for its function in intracellular cholesterol trafficking. J Lipid Res. 1995;36:2630-8 pubmed
    ..Sterol-carrier protein-2 (SCP2), an intracellular lipid binding protein, enhances the activation of enzymes involved in cholesterol metabolism...
  22. Camici O, Corazzi L. Import of phosphatidylethanolamine for the assembly of rat brain mitochondrial membranes. J Membr Biol. 1995;148:169-76 pubmed
    ..Phospholipase C hydrolyzed 25-30% of both PE radioactivity and mass of the outer mitochondrial membrane indicating an asymmetrical distribution of this lipid across the membrane. ..
  23. Kolodner R, Hall N, Lipford J, Kane M, Rao M, Morrison P, et al. Structure of the human MSH2 locus and analysis of two Muir-Torre kindreds for msh2 mutations. Genomics. 1994;24:516-26 pubmed
  24. Morin R, Brun M, Srikantaiah M. Effect of age and cholestyramine feeding on rat liver 3-hydroxy-3-methyl glutaryl CoA reductase, sterol carrier protein 1 and sterol carrier protein 2 activities. Lipids. 1982;17:507-10 pubmed
    ..Feeding of cholestryamine to adult rats increased the activities of the microsomal enzymes, sterol carrier protein 1 and sterol carrier protein 2 involved in the conversion of squalene to cholesterol. ..
  25. Chanderbhan R, Kharroubi A, Noland B, Scallen T, Vahouny G. Sterol carrier protein2: further evidence for its role in adrenal steroidogenesis. Endocr Res. 1986;12:351-70 pubmed
    Homogeneous rat liver sterol carrier protein (SCP2) has been implicated in adrenal steroidogenesis by studies utilizing as a model system various sub-cellular fractions of rat adrenals...
  26. McNamara B, Jefcoate C. The role of sterol carrier protein 2 in stimulation of steroidogenesis in rat adrenal mitochondria by adrenal cytosol. Arch Biochem Biophys. 1989;275:53-62 pubmed previously identified cytosolic constituents steroidogenesis activator peptide and sterol carrier protein 2 (SCP2)...
  27. Yamamoto R, Kallen C, Babalola G, Rennert H, Billheimer J, Strauss J. Cloning and expression of a cDNA encoding human sterol carrier protein 2. Proc Natl Acad Sci U S A. 1991;88:463-7 pubmed
    We report the cloning and expression of a cDNA encoding human sterol carrier protein 2 (SCP2). The 1...
  28. van Haren L, Teerds K, Ossendorp B, van Heusden G, Orly J, Stocco D, et al. Sterol carrier protein 2 (non-specific lipid transfer protein) is localized in membranous fractions of Leydig cells and Sertoli cells but not in germ cells. Biochim Biophys Acta. 1992;1124:288-96 pubmed
    The cellular and subcellular distribution of sterol carrier protein 2 (SCP2; nsL-TP) was reinvestigated in rat testicular cells by Western blotting and immunocytochemistry, using the affinity purified antibody against rat liver SCP2...