Gene Symbol: Rph3a
Description: rabphilin 3A
Alias: rabphilin-3A, exophilin-1, rabphilin 3A homolog
Species: rat
Products:     Rph3a

Top Publications

  1. Deak F, Shin O, Tang J, Hanson P, Ubach J, Jahn R, et al. Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion. EMBO J. 2006;25:2856-66 pubmed
  2. Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman E, et al. Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron. 1994;13:885-98 pubmed
    ..These results suggest that analogous to the membrane recruitment of raf by ras, rab3A and rab3C may function in recruiting rabphilin-3A to the synaptic vesicle membrane in a GTP-dependent manner. ..
  3. Valentijn J, van Weeren L, Ultee A, Koster A. Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking. Am J Physiol Gastrointest Liver Physiol. 2007;293:G165-77 pubmed
    ..Taken together, the present data suggest that Rab3D and rabphilin may regulate the secretory pathway at a much earlier stage than what has hitherto been assumed. ..
  4. Tsuboi T, Kanno E, Fukuda M. The polybasic sequence in the C2B domain of rabphilin is required for the vesicle docking step in PC12 cells. J Neurochem. 2007;100:770-9 pubmed
    ..These results indicate that the polybasic sequence in the C2B domain functions as an effector domain for SNAP-25 and controls the number of 'releasable' vesicles docked to the plasma membrane. ..
  5. Baldini G, Martelli A, Tabellini G, Horn C, Machaca K, Narducci P, et al. Rabphilin localizes with the cell actin cytoskeleton and stimulates association of granules with F-actin cross-linked by {alpha}-actinin. J Biol Chem. 2005;280:34974-84 pubmed
    ..These results suggest that rabphilin, by interacting with alpha-actinin, organizes the cell cytoskeleton to facilitate granule localization within F-actin-rich regions. ..
  6. Foletti D, Scheller R. Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci. 2001;21:5461-72 pubmed
    ..We propose that the phosphorylation of rabphilin regulates neuronal activity through development and in a synapse-specific manner. ..
  7. Kato M, Sasaki T, Ohya T, Nakanishi H, Nishioka H, Imamura M, et al. Physical and functional interaction of rabphilin-3A with alpha-actinin. J Biol Chem. 1996;271:31775-8 pubmed
    ..It has been shown that reorganization of actin filaments is also involved in Ca2+-dependent exocytosis. Therefore, rabphilin-3A may serve as a linker for Rab3A and cytoskeleton. ..
  8. Imazumi K, Sasaki T, Takahashi K, Takai Y. Identification of a rabphilin-3A-interacting protein as GTP cyclohydrolase I in PC12 cells. Biochem Biophys Res Commun. 1994;205:1409-16 pubmed
    ..The amino acid sequence analysis of this 30 KDa protein revealed that it is GTP cyclohydrolase I. ..
  9. Fukuda M, Kanno E, Yamamoto A. Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. J Biol Chem. 2004;279:13065-75 pubmed

More Information


  1. Montaville P, Schlicker C, Leonov A, Zweckstetter M, Sheldrick G, Becker S. The C2A-C2B linker defines the high affinity Ca(2+) binding mode of rabphilin-3A. J Biol Chem. 2007;282:5015-25 pubmed
    ..Therefore, this unprecedented Ca(2+) binding mode not only shows how a C2 domain increases its intrinsic Ca(2+) affinity, but also provides the structural base for an atypical protein-Ca(2+)-phospholipid binding mode of rabphilin-3A. ..
  2. Coudevylle N, Montaville P, Leonov A, Zweckstetter M, Becker S. Structural determinants for Ca2+ and phosphatidylinositol 4,5-bisphosphate binding by the C2A domain of rabphilin-3A. J Biol Chem. 2008;283:35918-28 pubmed publisher
    ..Our data provide detailed structural insight into the functional properties of the rabphilin-3A C2A domain and reveal for the first time the structural determinants of a target-activated messenger affinity mechanism. ..
  3. Stanic J, Carta M, Eberini I, Pelucchi S, Marcello E, Genazzani A, et al. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nat Commun. 2015;6:10181 pubmed publisher
    ..Here, we identify Rabphilin 3A (Rph3A) as a new GluN2A subunit-binding partner...
  4. Lonart G, Sudhof T. Characterization of rabphilin phosphorylation using phospho-specific antibodies. Neuropharmacology. 2001;41:643-9 pubmed
    ..Our data support the notion that dissociated rabphilin is a synaptic phosphoprotein in vivo that may play a role in the regulation of nerve terminal protein-protein interactions. ..
  5. Ostermeier C, Brunger A. Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell. 1999;96:363-74 pubmed
    ..RabCDRs could be major determinants of effector specificity during vesicle trafficking and fusion. ..
  6. Rastaldi M, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, et al. Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. Am J Pathol. 2003;163:889-99 pubmed
    ..The altered expression observed in mouse and human proteinuric diseases suggests a possible role for these molecules in glomerulopathies. ..
  7. Lee J, Huang P, Lin Y, Kao L, Huang C, Kao F, et al. In-depth fluorescence lifetime imaging analysis revealing SNAP25A-Rabphilin 3A interactions. Microsc Microanal. 2008;14:507-18 pubmed publisher
    ..synaptosomal associated protein of 25 kDa (SNAP25A) and red fluorescence protein (mRFP)-labeled Rabphillin 3A (RPH3A) were co-expressed in PC12 cells as the FRET donor and acceptor, respectively...
  8. Willshaw A, Grant K, Yan J, Rockliffe N, Ambavarapu S, Burdyga G, et al. Identification of a novel protein complex containing annexin A4, rabphilin and synaptotagmin. FEBS Lett. 2004;559:13-21 pubmed
    ..Given that rabphilin and synaptotagmin are synaptic vesicle proteins involved in neurotransmitter release, the identification of this complex suggests that annexin A4 may play a role in synaptic exocytosis. ..
  9. Biadene M, Montaville P, Sheldrick G, Becker S. Structure of the C2A domain of rabphilin-3A. Acta Crystallogr D Biol Crystallogr. 2006;62:793-9 pubmed
    ..In comparison, the structurally very similar C2A domain of synaptotagmin I has a highly acidic electrostatic surface, suggesting completely unrelated functions for these two C2A domains. ..
  10. Montaville P, Coudevylle N, Radhakrishnan A, Leonov A, Zweckstetter M, Becker S. The PIP2 binding mode of the C2 domains of rabphilin-3A. Protein Sci. 2008;17:1025-34 pubmed publisher
    ..These different PIP2 headgroup recognition modes suggest that PIP2 is a target of the C2A domain of rabphilin-3A while this phospholipid is an effector of the C2B domain. ..
  11. Handley M, Burgoyne R. The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells. Biochem Biophys Res Commun. 2008;373:275-81 pubmed publisher