Rock2

Summary

Gene Symbol: Rock2
Description: Rho-associated coiled-coil containing protein kinase 2
Alias: ROCK-II, ROK, ROKalpha, Rho-associated coiled-coil forming kinase 2, RhoA - binding serine/threosine kinase alpha (ROK - alpha), p150 ROK-alpha, p164 ROCK-2, rho-associated protein kinase 2, rho-associated, coiled-coil-containing protein kinase 2, rho-associated, coiled-coil-containing protein kinase II, rhoA-binding kinase 2
Species: rat

Top Publications

  1. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
  2. doi Aldosterone induction of hepatic stellate cell contraction through activation of RhoA/ROCK-2 signaling pathway
    Hongli Ji
    Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Regul Pept 169:13-20. 2011
  3. ncbi A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Singapore
    J Biol Chem 270:29051-4. 1995
  4. ncbi Vasoconstrictor effect of endothelin-1 on hypertensive pulmonary arterial smooth muscle involves Rho-kinase and protein kinase C
    Scott A Barman
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Lung Cell Mol Physiol 293:L472-9. 2007
  5. ncbi Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells
    Najma Begum
    Diabetes Research Laboratory, Winthrop University Hospital, Mineola, New York 11501, USA
    J Biol Chem 277:6214-22. 2002
  6. pmc Spironolactone lowers portal hypertension by inhibiting liver fibrosis, ROCK-2 activity and activating NO/PKG pathway in the bile-duct-ligated rat
    Wei Luo
    Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Southern Medical University, Nanfang Hospital, Guangzhou, China
    PLoS ONE 7:e34230. 2012
  7. ncbi G protein-coupled estrogen receptor1 (GPER1) may mediate Rho-kinase (ROCK-2) up-regulation in coronary endothelial cells
    A H Kurt
    Department of Pharmacology, Mersin University, Mersin, Turkey
    Endocr Regul 47:75-84. 2013
  8. doi Rho-associated protein kinase isoforms stimulate proliferation of vascular smooth muscle cells through ERK and induction of cyclin D1 and PCNA
    Ying Zhao
    Key Laboratory of Molecular Biology of Liaoning Province, Dalian Medical University, Dalian 116044, China
    Biochem Biophys Res Commun 432:488-93. 2013
  9. doi Molecular pathways regulating contractility in rat uterus through late gestation and parturition
    Michael J Taggart
    Institute of Cellular Medicine, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom
    Am J Obstet Gynecol 207:76.e15-24. 2012
  10. doi RanBPM is expressed in synaptic layers of the mammalian retina and binds to metabotropic glutamate receptors
    Angela Seebahn
    Institut für Biochemie Emil Fischer Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstr 17, 91054 Erlangen, Germany
    FEBS Lett 582:2453-7. 2008

Research Grants

Scientific Experts

  • Nikki L Jernigan
  • Alessandro Cavarape
  • Jay S Naik
  • Hideo Uehara
  • Yi Ju Lee
  • Najma Begum
  • Scott A Barman
  • Wei Luo
  • Ying Zhao
  • A H Kurt
  • Michael J Taggart
  • Xu Li
  • Ying Meng
  • Dominik Kentrup
  • Winnie Waichi Shum
  • Hongli Ji
  • Jun Ma
  • Nongxiu Ning
  • Emily Z Xu
  • Yuepeng Wang
  • Sylvester Tumusiime
  • Go Anegawa
  • Takehiro Ogata
  • Angela Seebahn
  • Wenyu Wen
  • Shabaz A Hamid
  • Preeti Ahuja
  • Chirag A Patel
  • Kyan J Allahdadi
  • Atsuko Yoneda
  • Liming Jin
  • Akira Nakamura
  • Yuhong Song
  • Songhee Jeon
  • I Un
  • Kazuhiro Kohama
  • Ying Gao
  • Haishuang Lin
  • Xiaoqing Wei
  • Ying Cui
  • K Buyukafsar
  • S Ulker
  • R N Tiftik
  • Miao Lv
  • Yuanhua Qin
  • Shan Huang
  • T Leung
  • Kai Cui
  • Chang Hui Yu
  • Chun Qiu Pan
  • Bryan F Mitchell
  • Hong Li Ji
  • Patrice Arthur
  • Barbara Zielnik
  • Zhen Shu Zhang
  • Li Ming Xiao
  • Shu Yuan Ni
  • Dennis Brown
  • Zhenshu Zhang
  • Alexander Grabner
  • Hermann Pavenstadt
  • Bayram Edemir
  • Clémence Belleannee
  • Otmar Schober
  • Eberhard Schlatter
  • Uta Schnöckel
  • Mary McKee
  • Sylvie Breton
  • Stefan Reuter
  • Bing Xiao
  • Pingsheng Wu
  • Michael Schäfers
  • Nicolas Da Silva
  • Xiaolan Zhang
  • Eckhart Büssemaker
  • Patrick J McNamara
  • Julijana Ivanovska
  • JinHua Zheng
  • Lei Zhang
  • Ruifang Wang
  • Crystal Kantores
  • Guiting Lin
  • Robert P Jankov
  • Brian P Kavanagh
  • Ching Shwun Lin
  • Daling Zhu
  • Tom F Lue
  • Shujun Liang
  • Jing Jiang
  • Xiaodong Zheng

Detail Information

Publications35

  1. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ..We recently reported on a p150 serine/threonine kinase (termed ROK alpha) binding RhoA only in its active GTP-bound state and on its cDNA; introduction of RhoA into HeLa cells ..
  2. doi Aldosterone induction of hepatic stellate cell contraction through activation of RhoA/ROCK-2 signaling pathway
    Hongli Ji
    Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
    Regul Pept 169:13-20. 2011
    ..This finding may provide a potential therapeutic target for control of cirrhosis and portal hypertension...
  3. ncbi A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Singapore
    J Biol Chem 270:29051-4. 1995
    ..Antibody raised against the kinase domain of the predicted protein, termed ROK alpha (for ROK alpha, RhoA-binding kinase), recognized a ubiquitous 150-kDa protein...
  4. ncbi Vasoconstrictor effect of endothelin-1 on hypertensive pulmonary arterial smooth muscle involves Rho-kinase and protein kinase C
    Scott A Barman
    Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912, USA
    Am J Physiol Lung Cell Mol Physiol 293:L472-9. 2007
    ..These results suggest that both PKC and Rho/Rho-kinase mediate the heightened pulmonary vascular response to ET-1 in hypertensive pulmonary arterial smooth muscle...
  5. ncbi Active Rho kinase (ROK-alpha ) associates with insulin receptor substrate-1 and inhibits insulin signaling in vascular smooth muscle cells
    Najma Begum
    Diabetes Research Laboratory, Winthrop University Hospital, Mineola, New York 11501, USA
    J Biol Chem 277:6214-22. 2002
    ..In control VSMCs, insulin inactivates ROK-alpha, the major Rho kinase isoform in VSMCs, and inhibits thrombin-induced increase in ROK-alpha association with ..
  6. pmc Spironolactone lowers portal hypertension by inhibiting liver fibrosis, ROCK-2 activity and activating NO/PKG pathway in the bile-duct-ligated rat
    Wei Luo
    Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Southern Medical University, Nanfang Hospital, Guangzhou, China
    PLoS ONE 7:e34230. 2012
    ..However, the mechanisms remain unclear. The present study aimed to investigate the role of spionolactone on liver cirrhosis and portal hypertension...
  7. ncbi G protein-coupled estrogen receptor1 (GPER1) may mediate Rho-kinase (ROCK-2) up-regulation in coronary endothelial cells
    A H Kurt
    Department of Pharmacology, Mersin University, Mersin, Turkey
    Endocr Regul 47:75-84. 2013
    ..Effect of estrogenic compounds and 17β-estradiol (E2), which induces endothelial cell motility, was investigated on ROCK-2 expression in rat coronary vascular endothelial cells (CVEC)...
  8. doi Rho-associated protein kinase isoforms stimulate proliferation of vascular smooth muscle cells through ERK and induction of cyclin D1 and PCNA
    Ying Zhao
    Key Laboratory of Molecular Biology of Liaoning Province, Dalian Medical University, Dalian 116044, China
    Biochem Biophys Res Commun 432:488-93. 2013
    ..We have reported that ROCK1, rather than ROCK2, induces platelet-derived growth factor (PDGF)-BB-stimulated migration of VSMCs...
  9. doi Molecular pathways regulating contractility in rat uterus through late gestation and parturition
    Michael J Taggart
    Institute of Cellular Medicine, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom
    Am J Obstet Gynecol 207:76.e15-24. 2012
    ..Our objective was to assess expression of key constituents of these pathways, in alliance with contractile function, through late gestation and during term and preterm labor...
  10. doi RanBPM is expressed in synaptic layers of the mammalian retina and binds to metabotropic glutamate receptors
    Angela Seebahn
    Institut für Biochemie Emil Fischer Zentrum, Friedrich Alexander Universitat Erlangen Nurnberg, Fahrstr 17, 91054 Erlangen, Germany
    FEBS Lett 582:2453-7. 2008
    ..RanBPM interacts with mGlu2 and other group II and group III receptors, except mGlu6. Our data suggest that RanBPM might be associated with mGlu receptors at synaptic sites...
  11. pmc Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury
    Dominik Kentrup
    Department of Internal Medicine D, Experimental Nephrology, University of Munster, Munster, Germany
    PLoS ONE 6:e26419. 2011
    ..We conclude from our study that ROCK-inhibition by hydroxyfasudil significantly improves kidney function in a rat model of acute renal IRI and is therefore a potential new therapeutic option in humans...
  12. ncbi Vessel- and vasoconstrictor-dependent role of rho/rho-kinase in renal microvascular tone
    Akira Nakamura
    Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan
    J Vasc Res 40:244-51. 2003
    ....
  13. pmc Regulation of ROCKII by localization to membrane compartments and binding to DynaminI
    Sylvester Tumusiime
    Department of Pharmacology and Experimental Therapeutics, LSU Health Sciences Center, 1901 Perdido St, 5th Floor MEB, New Orleans, LA 70112, USA
    Biochem Biophys Res Commun 381:393-6. 2009
    ..Our data suggest that ROCKII is regulated by localization to specific membrane compartments and its novel binding partner, Dynamin1...
  14. doi Extracellular matrix controls insulin signaling in mammary epithelial cells through the RhoA/Rok pathway
    Yi Ju Lee
    Institute of Immunology, Chung Shan Medical University, Taichung, Taiwan, ROC
    J Cell Physiol 220:476-84. 2009
    ..The amount and the activity of Rho kinase (Rok) associated with IRS-1 are greater in these cells, leading to serine phosphorylation of IRS-1...
  15. doi ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat
    Jun Ma
    Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical University, Nangang District, Harbin, Heilongjiang, PR China
    J Cell Physiol 222:82-94. 2010
    ..Thus, these findings indicate that 15-HETE protects PASMC from apoptosis, contributing to pulmonary vascular medial thickening, and the effect is, at least in part, mediated via the ROCK pathway...
  16. doi Rescue treatment with a Rho-kinase inhibitor normalizes right ventricular function and reverses remodeling in juvenile rats with chronic pulmonary hypertension
    Emily Z Xu
    Clinical Integrative Biology, Sunnybrook Research Institute, University of Toronto, Toronto, Ontario, Canada
    Am J Physiol Heart Circ Physiol 299:H1854-64. 2010
    ..In addition, our data suggest that ROCK-II may be dominant in enhancing survival of pulmonary arterial smooth muscle...
  17. pmc Effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells
    Nongxiu Ning
    Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143, USA
    Urology 76:1517.e6-11. 2010
    ..To investigate the effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells (USMCs)...
  18. pmc Regulation of V-ATPase recycling via a RhoA- and ROCKII-dependent pathway in epididymal clear cells
    Winnie Waichi Shum
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 301:C31-43. 2011
    ..We propose that inhibition of RhoA-ROCKII might be part of the intracellular signaling cascade that is triggered upon agonist-induced apical membrane V-ATPase accumulation...
  19. ncbi RhoA and Rho kinase-dependent phosphorylation of moesin at Thr-558 in hippocampal neuronal cells by glutamate
    Songhee Jeon
    Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440 746, Korea
    J Biol Chem 277:16576-84. 2002
    ....
  20. ncbi Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension
    M Uehata
    Discovery Research Tokyo, Yoshitomi Pharmaceutical Industries Ltd, Saitama, Japan
    Nature 389:990-4. 1997
    ..Our findings indicate that p160ROCK-mediated Ca2+ sensitization is involved in the pathophysiology of hypertension and suggest that compounds that inhibit this process might be useful therapeutically...
  21. pmc ROCK isoform regulation of myosin phosphatase and contractility in vascular smooth muscle cells
    Yuepeng Wang
    Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    Circ Res 104:531-40. 2009
    ..Two ROCK isoforms, ROCK1 and ROCK2, are expressed in many tissues, yet the isoform-specific roles of ROCK1 and ROCK2 in vascular smooth muscle and ..
  22. pmc Structure basis and unconventional lipid membrane binding properties of the PH-C1 tandem of rho kinases
    Wenyu Wen
    Department of Biochemistry, Molecular Neuroscience Center, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 283:26263-73. 2008
    ..Finally, the analysis of all split PH domains with known structures indicates that split PH domains represent a unique class of tandem protein modules, each possessing distinct structural and functional features...
  23. pmc Reactive oxygen species mediate RhoA/Rho kinase-induced Ca2+ sensitization in pulmonary vascular smooth muscle following chronic hypoxia
    Nikki L Jernigan
    Vascular Physiology Group, Dept of Cell Biology and Physiology, Univ of New Mexico Health Sciences Center, MSC 08 4750, 1 Univ of New Mexico, Albuquerque, NM 87131 0001, USA
    Am J Physiol Lung Cell Mol Physiol 295:L515-29. 2008
    Recent evidence supports a prominent role for Rho kinase (ROK)-mediated pulmonary vasoconstriction in the development and maintenance of chronic hypoxia (CH)-induced pulmonary hypertension...
  24. ncbi Chronic hypoxia augments protein kinase G-mediated Ca2+ desensitization in pulmonary vascular smooth muscle through inhibition of RhoA/Rho kinase signaling
    Nikki L Jernigan
    Vascular Physiology Group, Department of Cell Biology MSC 08 4750, 1 Univ of New Mexico, Albuquerque, NM 87131 0001, USA
    Am J Physiol Lung Cell Mol Physiol 287:L1220-9. 2004
    ..following CH is a function of VSM myofilament Ca(2+) desensitization via inhibition of the RhoA/Rho kinase (ROK) pathway...
  25. ncbi Enhanced role for RhoA-associated kinase in adrenergic-mediated vasoconstriction in gracilis arteries from obese Zucker rats
    Jay S Naik
    University of Mississippi Medical Center, Deptartment of Physiology and Biophysics, 2500 N State St, Jackson, MS 39216, USA
    Am J Physiol Regul Integr Comp Physiol 290:R154-61. 2006
    ..Total RhoA and ROKalpha protein expressions were similar between groups...
  26. ncbi Elevated RhoA/Rho-kinase activity in the aged rat penis: mechanism for age-associated erectile dysfunction
    Liming Jin
    Department of Urology, Johns Hopkins University, Baltimore, Maryland 21287, USA
    FASEB J 20:536-8. 2006
    ..05). Taken together, these data suggest that impaired erectile function during the aging process involves increased RhoA/Rho-kinase signaling, and this pathway may be exploited for the treatment of age-associated ED...
  27. pmc Fibronectin matrix assembly requires distinct contributions from Rho kinases I and -II
    Atsuko Yoneda
    Division of Biomedical Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    Mol Biol Cell 18:66-75. 2007
    ..reflection microscopy revealed that a submembranous pool of myosin light chain in control cells was missing in ROCK II-deficient cells and replaced by stress fibers...
  28. ncbi Rho kinase activation plays a major role as a mediator of irreversible injury in reperfused myocardium
    Shabaz A Hamid
    Royal Veterinary College, University of London, London, UK
    Am J Physiol Heart Circ Physiol 292:H2598-606. 2007
    ..Inhibition of ROCK at reperfusion onset limits infarct size through an Akt/eNOS-dependent mechanism, suggesting that ROCK activation at reperfusion may be deleterious through suppression of the RISK pathway...
  29. ncbi Re-expression of proteins involved in cytokinesis during cardiac hypertrophy
    Preeti Ahuja
    Institute of Cell Biology, ETH Zurich Honggerberg, CH 8093 Zurich, Switzerland
    Exp Cell Res 313:1270-83. 2007
    ..we demonstrate for the first time that small GTPases like RhoA along with their downstream effectors like ROCK I, ROCK II and Citron Kinase show a developmental stage specific expression in heart, with high levels being expressed in ..
  30. ncbi Cellular regulation of basal tone in internal anal sphincter smooth muscle by RhoA/ROCK
    Chirag A Patel
    Dept of Medicine, Division of Gastroenterology and Hepatology, Thomas Jefferson Univ, 1025 Walnut St, Rm 901 College, Philadelphia, PA 19107, USA
    Am J Physiol Gastrointest Liver Physiol 292:G1747-56. 2007
    ..We conclude that RhoA/ROCK play a critical role in maintenance of spontaneous tone in the IAS SMC via inhibition of myosin light chain phosphatase...
  31. ncbi Rho-kinase inhibition blunts renal vasoconstriction induced by distinct signaling pathways in vivo
    Alessandro Cavarape
    Department of Experimental and Clinical Pathology and Medicine DPMSC, University of Udine, Udine, Italy
    J Am Soc Nephrol 14:37-45. 2003
    ..Recent evidence suggests that distinct signaling cascades of vasoactive hormones interact with the Rho/Rho kinase (ROK) pathway, affecting the activity of MLCP...
  32. ncbi ROK contribution to endothelin-mediated contraction in aorta and mesenteric arteries following intermittent hypoxia/hypercapnia in rats
    Kyan J Allahdadi
    Department of Cell Biology and Physiology, Vascular Physiology Group, University of New Mexico, Albuquerque, New Mexico 87131, USA
    Am J Physiol Heart Circ Physiol 293:H2911-8. 2007
    ..Because Rho-associated kinase (ROK) can mediate Ca(2+) sensitization, we hypothesized that augmented vasoconstrictor sensitivity to ET-1 in arteries ..
  33. ncbi ROCK-II-induced membrane blebbing and chromatin condensation require actin cytoskeleton
    Yuhong Song
    Department of Medicine, Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, California 900095 1678, USA
    Exp Cell Res 278:45-52. 2002
    Ectopic expression of ROCK II (Rho kinase II or ROKalpha), an effector of Rho GTPase, induces membrane blebbing and chromatin condensation...
  34. pmc MURC, a muscle-restricted coiled-coil protein that modulates the Rho/ROCK pathway, induces cardiac dysfunction and conduction disturbance
    Takehiro Ogata
    Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, Kyoto 606 8507, Japan
    Mol Cell Biol 28:3424-36. 2008
    ..These findings indicate that MURC modulates RhoA signaling and that MURC plays an important role in the development of cardiac dysfunction and conduction disturbance with increased vulnerability to atrial arrhythmias...
  35. doi Defective endothelial nitric oxide synthase signaling is mediated by rho-kinase activation in rats with secondary biliary cirrhosis
    Go Anegawa
    Department of Surgery and Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan
    Hepatology 47:966-77. 2008
    ....

Research Grants3

  1. Functional hyperemia during metabolic syndrome
    Jay Naik; Fiscal Year: 2005
    ....
  2. Role of DEG/ENaC in the renal myogenic response
    NIKKI JERNIGAN; Fiscal Year: 2005
    ..abstract_text> ..