Gene Symbol: Rasa1
Description: RAS p21 protein activator 1
Alias: GAPX, Rasa, ras GTPase-activating protein 1, GAP, GTPase-activating protein, RAS p21 protein activator (GTPase activating protein) 1, p120GAP, rasGAP
Species: rat
Products:     Rasa1

Top Publications

  1. Kazlauskas A, Ellis C, Pawson T, Cooper J. Binding of GAP to activated PDGF receptors. Science. 1990;247:1578-81 pubmed
    ..products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused ..
  2. McGlade J, Brunkhorst B, Anderson D, Mbamalu G, Settleman J, Dedhar S, et al. The N-terminal region of GAP regulates cytoskeletal structure and cell adhesion. EMBO J. 1993;12:3073-81 pubmed
    Ras GTPase activating protein (GAP) possesses a C-terminal domain that interacts with GTP-bound Ras, and an N-terminal region containing two SH2 domains and an SH3 domain...
  3. Rottapel R, Turck C, Casteran N, Liu X, Birnbaum D, Pawson T, et al. Substrate specificities and identification of a putative binding site for PI3K in the carboxy tail of the murine Flt3 receptor tyrosine kinase. Oncogene. 1994;9:1755-65 pubmed
    ..b>GAP, Vav, Shc, and to a lesser extent PLC gamma become tyrosine-phosphorylated but no in vivo association with the ..
  4. Wainwright M, Brennan L, Dizon M, Black S. p21ras activation following hypoxia-ischemia in the newborn rat brain is dependent on nitric oxide synthase activity but p21ras does not contribute to neurologic injury. Brain Res Dev Brain Res. 2003;146:79-85 pubmed
    ..Ras does not, however, contribute to the pathophysiologic NO-dependent mechanisms of neurologic injury in this model. ..
  5. Liu J, Fitzli D, Liu M, Tseu I, Caniggia I, Rotin D, et al. PDGF-induced glycosaminoglycan synthesis is mediated via phosphatidylinositol 3-kinase. Am J Physiol. 1998;274:L702-13 pubmed
    ..On ligand-induced tyrosine phosphorylation, PDGFR associated with phospholipase C (PLC)-gamma 1, Ras GTPase activating protein (RasGAP), and phosphatidylinositol 3-kinase (PI3K) but not with the Syp-growth factor receptor-bound ..
  6. Moran M, Polakis P, McCormick F, Pawson T, Ellis C. Protein-tyrosine kinases regulate the phosphorylation, protein interactions, subcellular distribution, and activity of p21ras GTPase-activating protein. Mol Cell Biol. 1991;11:1804-12 pubmed
    The p21ras GTPase-activating protein (GAP) down-regulates p21ras by stimulating its intrinsic GTPase activity. GAP is found predominantly as a monomer in the cytosol of normal cells...
  7. Gibbs J, Marshall M, Scolnick E, Dixon R, Vogel U. Modulation of guanine nucleotides bound to Ras in NIH3T3 cells by oncogenes, growth factors, and the GTPase activating protein (GAP). J Biol Chem. 1990;265:20437-42 pubmed
    ..Ras biochemical activity involves regulation of a GTP/GDP cycle and the GTPase activating protein (GAP)...
  8. Yao R, Cooper G. Regulation of the Ras signaling pathway by GTPase-activating protein in PC12 cells. Oncogene. 1995;11:1607-14 pubmed
    We have investigated the role of Ras GTPase-activating protein (GAP) in NGF-induced neuronal differentiation by overexpressing both wild-type and membrane-targeted GAP in PC12 cells...
  9. Nakagawa Y, Purushotham K, Wang P, Fischer J, Dunn W, Schneyer C, et al. Alterations in the subcellular distribution of p21ras-GTPase activating protein in proliferating rat acinar cells. Biochem Biophys Res Commun. 1992;187:1172-9 pubmed
    ..Treatment with these agents caused an increase in tyrosine phosphorylation of p21ras-GTPase activating protein (GAP)...

Scientific Experts

More Information


  1. Ranganna K, Joshi T, Yatsu F. Sodium butyrate inhibits platelet-derived growth factor-induced proliferation of vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 1995;15:2273-83 pubmed
    ..The activated beta-PDGFR physically associated and phosphorylated signaling molecules such as ras-GTPase activating protein (GAP) and phospholipase C gamma (PLC gamma)...
  2. Wang J, Ruan K. miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1. Biochem Biophys Res Commun. 2010;402:222-7 pubmed publisher
    ..The bioinformatic analysis indicated that one of miR-335's putative targets is RASA1, a known gene related to cell proliferation and anti-apoptosis, which may contribute to the epididymal development...
  3. Durrington H, Firth H, Patient C, Belham M, Jayne D, Burrows N, et al. A novel RASA1 mutation causing capillary malformation-arteriovenous malformation (CM-AVM) presenting during pregnancy. Am J Med Genet A. 2013;161A:1690-4 pubmed publisher
    ..CM-AVM) is a newly recognized clinical entity caused by mutation of the RASA1 gene, which encodes p120-RasGAP. Here we describe, for the first time, a patient with CM-AVM presenting during the late stages of pregnancy with ..
  4. Yue Y, Lypowy J, Hedhli N, Abdellatif M. Ras GTPase-activating protein binds to Akt and is required for its activation. J Biol Chem. 2004;279:12883-9 pubmed
    b>RasGAP (Ras GTPase-activating protein) is a negative regulator as well as a downstream effector of Ras. To identify partners of RasGAP we used it as the bait in a yeast two-hybrid screen...
  5. Chang J, Kobayashi M, Wang D, Maruta H, Iwashita S. Two regions with differential growth-modulating activity in the N-terminal domain of ras GTPase-activating protein (p120GAP) src homology and Gly-Ala-Pro-rich regions. Eur J Biochem. 1995;232:691-9 pubmed
    Ras GTPase-activating protein of 120 kDa (p120GAP) consists of a hydrophobic Gly-Ala-Pro-rich stretch and src homology 2 and 3 (SH2/SH3) domains in the N-terminal half, and a Ras GTPase-activating domain at the C-terminus...
  6. Davis A, Butt J, Walker J, Moss S, Gawler D. The Ca2+-dependent lipid binding domain of P120GAP mediates protein-protein interactions with Ca2+-dependent membrane-binding proteins. Evidence for a direct interaction between annexin VI and P120GAP. J Biol Chem. 1996;271:24333-6 pubmed
    ..In the Ras GTPase activating protein, P120(GAP), this domain has the ability to confer membrane association in response to intracellular Ca2+ ..
  7. Fujita Yoshigaki J, Shirouzu M, Koide H, Nishimura S, Yokoyama S. Identification of amino acid residues of Ras protein that are essential for signal-transducing activity but not for enhancement of GTPase activity by GAP. FEBS Lett. 1991;294:187-90 pubmed
    ..nucleotide-binding activity or GTPase activity in the absence or presence of bovine GTPase-activating protein (GAP)...
  8. Yoshida E, Atkinson T, Chakravarthy B. Neuroprotective gene expression profiles in ischemic cortical cultures preconditioned with IGF-1 or bFGF. Brain Res Mol Brain Res. 2004;131:33-50 pubmed
    ..such as growth factor signal transduction (CD44, DTR, DUSP6, EPS8, IGFBP3), DNA repair and transcription (FOXJ1), metabolic homeostasis (RASA1, SHMT2), cytoskeletal stability (MSN, MAPT) and cholesterol biosynthesis (FDFT1, FDPS).
  9. Chow A, Davis A, Gawler D. Identification of a novel protein complex containing annexin VI, Fyn, Pyk2, and the p120(GAP) C2 domain. FEBS Lett. 2000;469:88-92 pubmed
    p120(GAP) (RasGAP) has been proposed to function as both an inhibitor and effector of Ras...
  10. Chen K, Guo X, Ma D, Guo Y, Li Q, Yang D, et al. Dysregulation of HSG triggers vascular proliferative disorders. Nat Cell Biol. 2004;6:872-83 pubmed
    ..Thus, rHSG functions as a cell proliferation suppressor, whereas dysregulation of rHSG results in proliferative disorders. ..
  11. Yang J, Michod D, Walicki J, Murphy B, Kasibhatla S, Martin S, et al. Partial cleavage of RasGAP by caspases is required for cell survival in mild stress conditions. Mol Cell Biol. 2004;24:10425-36 pubmed
    ..This mechanism relies on the partial cleavage of RasGAP by caspase 3 into an amino-terminal fragment called fragment N...
  12. Abramson J, Pecht I. Clustering the mast cell function-associated antigen (MAFA) leads to tyrosine phosphorylation of p62Dok and SHIP and affects RBL-2H3 cell cycle. Immunol Lett. 2002;82:23-8 pubmed
    ..Concomitantly, their respective binding to RasGAP and Shc was increased...
  13. Eerola I, Boon L, Mulliken J, Burrows P, Dompmartin A, Watanabe S, et al. Capillary malformation-arteriovenous malformation, a new clinical and genetic disorder caused by RASA1 mutations. Am J Hum Genet. 2003;73:1240-9 pubmed
    ..A positional candidate gene, RASA1, encoding p120-RasGAP, was screened for mutations in 17 families...
  14. Boon L, Mulliken J, Vikkula M. RASA1: variable phenotype with capillary and arteriovenous malformations. Curr Opin Genet Dev. 2005;15:265-9 pubmed
    ..Candidate gene screening pinpointed mutations in RASA1 (p120-RASGAP) - a RasGTPase. RASA1 reverts active GTP-bound Ras into inactive GDP-bound form...
  15. Ott V, Tamir I, Niki M, Pandolfi P, Cambier J. Downstream of kinase, p62(dok), is a mediator of Fc gamma IIB inhibition of Fc epsilon RI signaling. J Immunol. 2002;168:4430-9 pubmed
    ..Concurrently, enhanced p62(dok) tyrosine phosphorylation and association with RasGAP are observed, suggesting that SHIP may mediate Fc gamma RIIB inhibitory function in mast cells via recruitment of ..
  16. Davis M, Catino J, Satoh T, Kaziro Y, Perkins L. Sequence of the cDNA encoding Ras GTPase-activating protein from rat. Gene. 1993;134:305-6 pubmed
    We cloned and sequenced a 3296-bp cDNA encoding the rat Ras GTPase-activating protein (GAP)...
  17. Maekawa M, Nakamura S, Hattori S. Purification of a novel ras GTPase-activating protein from rat brain. J Biol Chem. 1993;268:22948-52 pubmed
    GTPase-activating protein (GAP) and neurofibromin, a gene product of neurofibromatosis type I gene, have been identified as factors that stimulate GTPase activity of ras p21...
  18. Brott B, Decker S, Shafer J, Gibbs J, Jove R. GTPase-activating protein interactions with the viral and cellular Src kinases. Proc Natl Acad Sci U S A. 1991;88:755-9 pubmed
    GTPase-activating protein (GAP), which regulates the activities of Ras proteins, is implicated in mitogenic signal transduction by growth-factor receptors and oncoproteins with tyrosine kinase activity...
  19. Yang J, Walicki J, Abderrahmani A, Cornu M, Waeber G, Thorens B, et al. Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion. J Biol Chem. 2005;280:32835-42 pubmed
    ..b>RasGAP, a regulator of Ras and Rho GTPases, is an atypical caspase substrate, since it inhibits, rather than favors, ..
  20. Marandi S, De Keyser N, Saliez A, Maernoudt A, Sokal E, Stilmant C, et al. Insulin signal transduction in rat small intestine: role of MAP kinases in expression of mucosal hydrolases. Am J Physiol Gastrointest Liver Physiol. 2001;280:G229-40 pubmed
    ..substrates-1 (IRS-1), -2, and -4; 2) phospholipase C-isoenzyme-gamma; 3) the Ras-GTPase-activating protein (GAP) associated with Rho GAP and p62(Src); 4) the insulin receptor beta-subunit; 5) the p85 subunits of ..
  21. Friedman E, Gejman P, Martin G, McCormick F. Nonsense mutations in the C-terminal SH2 region of the GTPase activating protein (GAP) gene in human tumours. Nat Genet. 1993;5:242-7 pubmed
    b>GTPase Activating Protein (GAP) is involved in down-regulating normal ras proteins and in the signal transduction pathway of some growth factors...
  22. Margolis B, Li N, Koch A, Mohammadi M, Hurwitz D, Zilberstein A, et al. The tyrosine phosphorylated carboxyterminus of the EGF receptor is a binding site for GAP and PLC-gamma. EMBO J. 1990;9:4375-80 pubmed
    Phospholipase C-gamma (PLC-gamma) and GTPase activating protein (GAP) are substrates of EGF, PDGF and other growth factor receptors...
  23. Pixley F, Xiong Y, Yu R, Sahai E, Stanley E, Ye B. BCL6 suppresses RhoA activity to alter macrophage morphology and motility. J Cell Sci. 2005;118:1873-83 pubmed
    ..at the plasma membrane and a reduction in cell surface expression of the CSF1R that has been reported to recruit RasGAP to the plasma membrane...
  24. Queirós A, Eschen C, Fliegner D, Kararigas G, Dworatzek E, Westphal C, et al. Sex- and estrogen-dependent regulation of a miRNA network in the healthy and hypertrophied heart. Int J Cardiol. 2013;169:331-8 pubmed publisher
    ..e. Rasa1, Rasa2 and Spry1, which may all lead to cardiac fibrosis...