Rab3a

Summary

Gene Symbol: Rab3a
Description: RAB3A, member RAS oncogene family
Alias: RAB3, Ras-related small GTP binding protein 3A, ras-related protein Rab-3A
Species: rat

Top Publications

  1. doi Involvement of Rab3A in vesicle priming during exocytosis: interaction with Munc13-1 and Munc18-1
    Chien Chang Huang
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan, R O C
    Traffic 12:1356-70. 2011
  2. ncbi Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2
    Yaisa S Andrews-Zwilling
    Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann Rein Strasse 3, D 37075 Gottingen, Germany
    J Biol Chem 281:19720-31. 2006
  3. pmc Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion
    Ferenc Deak
    Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    EMBO J 25:2856-66. 2006
  4. pmc Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a
    Maria Pia Rastaldi
    Renal Immunopathology Laboratory, Associazione Nuova Nefrologia, c o San Carlo Borromeo Hospital, Via Pio II, 20153 Milan, Italy
    Am J Pathol 163:889-99. 2003
  5. pmc Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily
    Christelle Alory
    Departments of Cell and Molecular Biology and The Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, California 92130, USA
    Mol Biol Cell 14:3857-67. 2003
  6. ncbi Attenuation of zinc-induced neuronal death by the interaction of growth inhibitory factor with Rab3A in rat hippocampal neurons
    Ying Liu
    National Key Laboratory of Protein Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Neurosci Lett 358:149-52. 2004
  7. ncbi Chemical genetics identifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors
    Mark R Lackner
    Exelixis Inc, 170 Harbor Way, South San Francisco, California 94083, USA
    Cancer Cell 7:325-36. 2005
  8. pmc Real-time imaging of Rab3a and Rab5a reveals differential roles in presynaptic function
    Erin N Star
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    J Physiol 569:103-17. 2005
  9. ncbi Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs
    M Rupnik
    Lab of Neuroendocrinology Molecular Cell Physiology, Institute of Pathophysiology, School of Medicine, PO Box 2211, 1001 Ljubljana, Slovenia
    Am J Physiol Cell Physiol 292:C98-105. 2007
  10. ncbi Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy
    C C Lin
    Department of Life Sciences and Institute of Genome Sciences, National Yang Ming University, Taipei, Taiwan, ROC
    J Cell Physiol 211:316-26. 2007

Scientific Experts

  • Jae Bong Park
  • M P Rastaldi
  • Torsten Wollert
  • Chien Chang Huang
  • Nathan J Pavlos
  • Zhanxiang Wang
  • Mihaela Anitei
  • Reinhard Jahn
  • M Rupnik
  • Mark T W Handley
  • Fengyi Liang
  • C C Lin
  • Yaisa S Andrews-Zwilling
  • Ferenc Deak
  • Mark R Lackner
  • Erin N Star
  • Ying Liu
  • Christelle Alory
  • William E Balch
  • Toshiaki Sakisaka
  • Sharon C Francis
  • Thierry Coppola
  • H R Luo
  • G Tabellini
  • L P Haynes
  • John A Mercer
  • Lung Sen Kao
  • Michael S Cosgrove
  • Chung Chih Lin
  • Ying Lung Lee
  • Valarie E Vought
  • D William Provance
  • Anamika Patel
  • De Ming Yang
  • George M Langford
  • Tobias H Kloepper
  • John J E Chua
  • Silvio O Rizzoli
  • S Y Han
  • Dietmar Riedel
  • Mads Grønborg
  • Janina Boyken
  • Y Wang
  • Henning Urlaub
  • Rashmi Bansal
  • Ann E Cowan
  • Steven E Pfeiffer
  • Debbie C Thurmond
  • V Maienschein
  • J J Dumas
  • C Ostermeier
  • L K Bobanovic
  • Bin Zhang
  • Y N Huang
  • Robert D Burgoyne
  • Y S Tsai
  • Yajun Wu
  • T Kiauta
  • K H Lin
  • L Johannes
  • Wenbo Li
  • Eng Ang Ling
  • Junhong Tang
  • L S Kao
  • F Nothias
  • Jing Guo
  • M Kreft
  • K H Cheng
  • R Y Ong
  • Lee P Haynes
  • Yee Gek Chan
  • Qiong Cao
  • P Vernier
  • D M Yang
  • F Darchen
  • R Zorec
  • C C Huang
  • S Grilc
  • Haiying Chu
  • Nils Brose
  • Josep Rizo
  • Hiroshi Kawabe
  • Phyllis Hanson
  • Ege T Kavalali
  • Josep Ubach
  • Jiong Tang
  • Frederique Varoqueaux
  • Kerstin Reim
  • Ok Ho Shin
  • Thomas C Sudhof

Detail Information

Publications34

  1. doi Involvement of Rab3A in vesicle priming during exocytosis: interaction with Munc13-1 and Munc18-1
    Chien Chang Huang
    Institute of Biochemistry and Molecular Biology, National Yang Ming University, Taipei, Taiwan, R O C
    Traffic 12:1356-70. 2011
    b>Rab3A is a small G-protein of the Rab family that is involved in the late steps of exocytosis. Here, we studied the role of Rab3A and its relationship with Munc13-1 and Munc18-1 during vesicle priming...
  2. ncbi Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2
    Yaisa S Andrews-Zwilling
    Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann Rein Strasse 3, D 37075 Gottingen, Germany
    J Biol Chem 281:19720-31. 2006
    ..that the active zone recruitment of Munc13 isoforms Munc13-1 and ubMunc13-2 is regulated by their binding to the Rab3A-interacting molecule RIM1alpha, a key determinant of long term potentiation of synaptic transmission at mossy ..
  3. pmc Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion
    Ferenc Deak
    Center for Basic Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX 75390 9111, USA
    EMBO J 25:2856-66. 2006
    ..is catalyzed by assembly of synaptic SNARE complexes, and is regulated by the synaptic vesicle GTP-binding protein Rab3 that binds to RIM and to rabphilin...
  4. pmc Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a
    Maria Pia Rastaldi
    Renal Immunopathology Laboratory, Associazione Nuova Nefrologia, c o San Carlo Borromeo Hospital, Via Pio II, 20153 Milan, Italy
    Am J Pathol 163:889-99. 2003
    ..In neurons, the small guanosine triphosphatase Rab3A and its effector rabphilin-3A form a complex required for the correct docking of synaptic vesicles to their target ..
  5. pmc Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily
    Christelle Alory
    Departments of Cell and Molecular Biology and The Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, California 92130, USA
    Mol Biol Cell 14:3857-67. 2003
    ..We have now isolated REP mutant proteins that are able to partially function as both REP and GDI. These results provide molecular insight into the functional and evolutionary organization of the REP/GDI superfamily...
  6. ncbi Attenuation of zinc-induced neuronal death by the interaction of growth inhibitory factor with Rab3A in rat hippocampal neurons
    Ying Liu
    National Key Laboratory of Protein Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Neurosci Lett 358:149-52. 2004
    ..Neither the addition of recombinant GIF nor Rab3A alone could rescue these cells from death...
  7. ncbi Chemical genetics identifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors
    Mark R Lackner
    Exelixis Inc, 170 Harbor Way, South San Francisco, California 94083, USA
    Cancer Cell 7:325-36. 2005
    ..These findings validate RabGGT, and by extension endosomal function, as a therapeutically relevant target for modulation of apoptosis, and enhance our understanding of the mechanism of action of FTIs...
  8. pmc Real-time imaging of Rab3a and Rab5a reveals differential roles in presynaptic function
    Erin N Star
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    J Physiol 569:103-17. 2005
    We investigated the roles of two Rab-family proteins, Rab3a and Rab5a, in hippocampal synaptic transmission using real-time fluorescence imaging...
  9. ncbi Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs
    M Rupnik
    Lab of Neuroendocrinology Molecular Cell Physiology, Institute of Pathophysiology, School of Medicine, PO Box 2211, 1001 Ljubljana, Slovenia
    Am J Physiol Cell Physiol 292:C98-105. 2007
    ..microscope-based analysis of immunocytochemistry to show that rat melanotrophs contain about equal amounts of Rab3A and Rab3B transcripts as well as proteins...
  10. ncbi Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy
    C C Lin
    Department of Life Sciences and Institute of Genome Sciences, National Yang Ming University, Taipei, Taiwan, ROC
    J Cell Physiol 211:316-26. 2007
    b>Rab3A is a small G protein in the Rab3 subfamily, and is thought to act at late stage of exocytosis. However, the detailed mechanism of its action is not completely understood...
  11. pmc Differential dynamics of Rab3A and Rab27A on secretory granules
    Mark T W Handley
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK
    J Cell Sci 120:973-84. 2007
    We have assessed the dynamics of the association of Rab3A and Rab27A with secretory granules at various stages of their life in PC12 cells...
  12. ncbi RIM3gamma is a postsynaptic protein in the rat central nervous system
    Fengyi Liang
    Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, 4 Medical Drive, Singapore 117597
    J Comp Neurol 503:501-10. 2007
    RIMs (Rab3-interacting molecules) are synaptic proteins essential for neural transmission and plasticity. RIM1alpha has been implicated in membrane trafficking and regulation of secretory vesicle exocytosis in eukaryotic cells...
  13. doi Role for Rab3a in oligodendrocyte morphological differentiation
    Mihaela Anitei
    Department of Neuroscience, University of Connecticut Medical School, Farmington, Connecticut 06030 3401, USA
    J Neurosci Res 87:342-52. 2009
    b>Rab3a, a small GTPase important for exocytosis, is uniquely up-regulated as oligodendrocytes enter terminal differentiation and initiate myelin biosynthesis...
  14. pmc Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins
    Zhanxiang Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Cell Sci 122:893-903. 2009
    ....
  15. doi Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis
    Nathan J Pavlos
    Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    J Neurosci 30:13441-53. 2010
    ..iTRAQ) together with quantitative immunoblotting and fluorescence microscopy, we have determined the exocytotic (Rab3a, Rab3b, Rab3c, and Rab27b) and endocytic (Rab4b, Rab5a/b, Rab10, Rab11b, and Rab14) Rab machinery of SVs...
  16. pmc Myosin5a tail associates directly with Rab3A-containing compartments in neurons
    Torsten Wollert
    Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA
    J Biol Chem 286:14352-61. 2011
    ..A potential class of binding partners are Rab GTPases and Rab3A is known to associate with SVs and is involved in SV trafficking...
  17. pmc Rab-alphaGDI activity is regulated by a Hsp90 chaperone complex
    Toshiaki Sakisaka
    Departments of Cell and Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    EMBO J 21:6125-35. 2002
    ..is sensitive to the Hsp90 inhibitor geldanamycin (GA) and that GA inhibits the ability of alphaGDI to recycle Rab3A during neurotransmitter release...
  18. pmc Pancreatic beta-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis
    Thierry Coppola
    University of Lausanne, Institut de Biologie Cellulaire et de Morphologie, Lausanne, Switzerland 1005
    Mol Biol Cell 13:1906-15. 2002
    ..found to bind to two components of the secretory machinery of pancreatic beta-cells, the small GTP-binding protein Rab3 and the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-binding protein Munc-18...
  19. pmc Complete coding sequences of the ras related rab 3 and 4 cDNAs
    A Zahraoui
    INSERM U 248, Faculte de Medecine Lariboisiere Saint Louis, Paris, France
    Nucleic Acids Res 16:1204. 1988
  20. pmc Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A
    W H Brondyk
    Department of Pathology, Vermont Cancer Center, University of Vermont College of Medicine, Burlington 05405 0068
    Mol Cell Biol 15:1137-43. 1995
    b>Rab3A is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis...
  21. ncbi The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis
    M J De Hoop
    European Molecular Biology Laboratory, Cell Biology Program, Federal Republic of Germany
    Neuron 13:11-22. 1994
    ..These results show a role for Rab5a in axonal and dendritic endocytosis, and the presence of Rab5a on synaptic vesicles indicates that the axonal endosomes participate in the biogenesis of these vesicles...
  22. ncbi Rho proteins are localized with different membrane compartments involved in vesicular trafficking in anterior pituitary cells
    D Cussac
    I C N E UMR 9941 CNRS Institut Jean Roche Faculté de Médecine Nord, Marseille, France
    Mol Cell Endocrinol 119:195-206. 1996
    ..b>Rab3 was localized like a fraction of synaptophysin, suggesting a role for rab3 in the targeting of "synaptic-like' ..
  23. ncbi Isolation and characterization of a dual prenylated Rab and VAMP2 receptor
    I Martincic
    Department of Medicine and Biochemistry, Loeb Research Institute, University of Ottawa, Ottawa, Ontario K1Y 4E9, Canada
    J Biol Chem 272:26991-8. 1997
    ..Using the yeast two-hybrid screen, we have isolated a rat clone that interacts with Rab3A as well as with Rab1. The gene encodes a 20...
  24. ncbi Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A
    C Ostermeier
    The Howard Hughes Medical Institute and Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Cell 96:363-74. 1999
    The small G protein Rab3A plays an important role in the regulation of neurotransmitter release. The crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A was solved to 2.6 A resolution...
  25. ncbi Structural basis of activation and GTP hydrolysis in Rab proteins
    J J Dumas
    Program in Molecular Medicine, University of Massachusetts Medical Center, 373 Plantation Street, Worcester, MA 01605, USA
    Structure 7:413-23. 1999
    ..Despite conservation of critical residues involved in nucleotide binding and hydrolysis, Rab proteins exhibit low sequence identity with other GTPases, and the structural basis for Rab function remains poorly characterized...
  26. ncbi A plethora of presynaptic proteins associated with ATP-storing organelles in cultured astrocytes
    V Maienschein
    Biozentrum der J W Goethe Universität, AK Neurochemie, Frankfurt am Main, Germany
    Glia 26:233-44. 1999
    ..organellar association of a large variety of synaptic proteins (synaptobrevin II, synaptotagmin I, synaptophysin, rab3a, synapsin I, SNAP-25, and syntaxin I) and also of the ubiquitous cellubrevin...
  27. ncbi The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins
    Y Wang
    Center for Basic Neuroscience, Department of Molecular Genetics and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9111, USA
    J Biol Chem 275:20033-44. 2000
    ..RIM1 is localized close to the active zone at the synapse, where it interacts in a GTP-dependent manner with Rab3 located on synaptic vesicles...
  28. pmc Identification of Rab6 as an N-ethylmaleimide-sensitive fusion protein-binding protein
    S Y Han
    School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Biochem J 352:165-73. 2000
    ..Furthermore, we found that Rab3 and Rab4 can also associate with NSF and stimulate its ATPase activity...
  29. ncbi A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells
    L P Haynes
    Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom
    J Biol Chem 276:9726-32. 2001
    ..b>Rab3A is a neural/neuroendocrine-specific member of the Rab family involved in Ca(2+) -regulated exocytosis, where it ..
  30. ncbi GRAB: a physiologic guanine nucleotide exchange factor for Rab3A, which interacts with inositol hexakisphosphate kinase
    H R Luo
    Department of Neuroscience, School of Medicine, Johns Hopkins University, 725 N Wolfe Street, Baltimore, MD 21205, USA
    Neuron 31:439-51. 2001
    ..the identification, cloning, and characterization of a novel protein, GRAB (guanine nucleotide exchange factor for Rab3A), which interacts with both InsP6K1 and Rab3A, a Ras-like GTPase that regulates synaptic vesicle exocytosis...
  31. ncbi Coordinate regulation of catecholamine uptake by rab3 and phosphoinositide 3-kinase
    Sharon C Francis
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 277:7816-23. 2002
    ..b>rab3A and rab3B also increased NE uptake into large dense core vesicles in digitonin-permeabilized PC12 cells, which ..
  32. ncbi Localization of the small monomeric GTPases Rab3D and Rab3A in the AtT-20 rat pituitary cell line
    G Tabellini
    Dipartimento di Morfologia Umana Normale, Universita degli Studi di Trieste, Italy
    Eur J Histochem 45:347-56. 2001
    We investigated the cellular localization of the small GTPases Rab3D and Rab3A in AtT-20 cells treated with the drug Brefeldin A...
  33. pmc GTP binds to Rab3A in a complex with Ca2+/calmodulin
    Jae Bong Park
    Department of Biochemistry, College of Medicine, Hallym University, Kangwon Do, 200 702, South Korea
    Biochem J 362:651-7. 2002
    ..b>Rab3A is present as either the soluble or the SV membrane-bound form in neurons that are dependent on the GDP- or GTP-..
  34. pmc Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library
    N Touchot
    Institut National de la Sante et de la Recherche Medicale U 248, Faculte de Medecine Lariboisiere Saint Louis, Paris, France
    Proc Natl Acad Sci U S A 84:8210-4. 1987
    ..rab1 is a mammalian homolog of the yeast YPT gene, and the four rab genes constitute an additional branch of the ras gene superfamily that to our knowledge has not been described in higher eukaryotes...