Rab3a

Summary

Gene Symbol: Rab3a
Description: RAB3A, member RAS oncogene family
Alias: RAB3, ras-related protein Rab-3A, Ras-related small GTP binding protein 3A
Species: rat
Products:     Rab3a

Top Publications

  1. Sakane A, Manabe S, Ishizaki H, Tanaka Okamoto M, Kiyokage E, Toida K, et al. Rab3 GTPase-activating protein regulates synaptic transmission and plasticity through the inactivation of Rab3. Proc Natl Acad Sci U S A. 2006;103:10029-34 pubmed
    b>Rab3A small G protein is a member of the Rab family and is most abundant in the brain, where it is localized on synaptic vesicles. Evidence is accumulating that Rab3A plays a key role in neurotransmitter release and synaptic plasticity...
  2. Haynes L, Evans G, Morgan A, Burgoyne R. A direct inhibitory role for the Rab3-specific effector, Noc2, in Ca2+-regulated exocytosis in neuroendocrine cells. J Biol Chem. 2001;276:9726-32 pubmed
    ..b>Rab3A is a neural/neuroendocrine-specific member of the Rab family involved in Ca(2+) -regulated exocytosis, where it ..
  3. Wang Z, Thurmond D. Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins. J Cell Sci. 2009;122:893-903 pubmed publisher
  4. Xie C, Li J, Guo T, Yan Y, Tang C, Wang Y, et al. Rab3A is a new interacting partner of synaptotagmin I and may modulate synaptic membrane fusion through a competitive mechanism. Biochem Biophys Res Commun. 2014;444:491-5 pubmed publisher
    ..However, the exact mechanism has not been fully understood. In this study, Rab3A and synaptotagmin I, the most abundant isoforms of Rab3 and synaptotagmin, respectively, in brain were for the ..
  5. Zografou S, Basagiannis D, Papafotika A, Shirakawa R, Horiuchi H, Auerbach D, et al. A complete Rab screening reveals novel insights in Weibel-Palade body exocytosis. J Cell Sci. 2012;125:4780-90 pubmed publisher
    ..Apart from Rab3 and Rab27, we identified three additional Rabs, Rab15 (a previously reported endocytic Rab), Rab33 and Rab37, on ..
  6. Lin Y, Lin Y, Kuo Y, Wang Y, Kuo P. Identification and characterization of a novel Rab GTPase-activating protein in spermatids. Int J Androl. 2011;34:e358-67 pubmed publisher
    ..This MgcRabGAP is co-localized with its candidate substrate Rab3A at the acrosome/acroplaxome and neck regions of spermatids...
  7. Zahraoui A, Touchot N, Chardin P, Tavitian A. Complete coding sequences of the ras related rab 3 and 4 cDNAs. Nucleic Acids Res. 1988;16:1204 pubmed
  8. Lackner M, Kindt R, Carroll P, Brown K, Cancilla M, Chen C, et al. Chemical genetics identifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors. Cancer Cell. 2005;7:325-36 pubmed
    ..These findings validate RabGGT, and by extension endosomal function, as a therapeutically relevant target for modulation of apoptosis, and enhance our understanding of the mechanism of action of FTIs. ..
  9. Andrews Zwilling Y, Kawabe H, Reim K, Varoqueaux F, Brose N. Binding to Rab3A-interacting molecule RIM regulates the presynaptic recruitment of Munc13-1 and ubMunc13-2. J Biol Chem. 2006;281:19720-31 pubmed
    ..that the active zone recruitment of Munc13 isoforms Munc13-1 and ubMunc13-2 is regulated by their binding to the Rab3A-interacting molecule RIM1alpha, a key determinant of long term potentiation of synaptic transmission at mossy ..

Scientific Experts

More Information

Publications50

  1. Anitei M, Cowan A, Pfeiffer S, Bansal R. Role for Rab3a in oligodendrocyte morphological differentiation. J Neurosci Res. 2009;87:342-52 pubmed publisher
    b>Rab3a, a small GTPase important for exocytosis, is uniquely up-regulated as oligodendrocytes enter terminal differentiation and initiate myelin biosynthesis...
  2. Stobrawa S, Breiderhoff T, Takamori S, Engel D, Schweizer M, Zdebik A, et al. Disruption of ClC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus. Neuron. 2001;29:185-96 pubmed
    ..Mice almost lacking the hippocampus survive and show several behavioral abnormalities but are still able to acquire motor skills. ..
  3. Martincic I, Peralta M, Ngsee J. Isolation and characterization of a dual prenylated Rab and VAMP2 receptor. J Biol Chem. 1997;272:26991-8 pubmed
    ..Using the yeast two-hybrid screen, we have isolated a rat clone that interacts with Rab3A as well as with Rab1. The gene encodes a 20...
  4. De Hoop M, Huber L, Stenmark H, Williamson E, Zerial M, Parton R, et al. The involvement of the small GTP-binding protein Rab5a in neuronal endocytosis. Neuron. 1994;13:11-22 pubmed
    ..These results show a role for Rab5a in axonal and dendritic endocytosis, and the presence of Rab5a on synaptic vesicles indicates that the axonal endosomes participate in the biogenesis of these vesicles. ..
  5. Tang X, Xie C, Wang Y, Wang X. Localization of Rab3A-binding site on C2A domain of synaptotagmin I to reveal its regulatory mechanism. Int J Biol Macromol. 2017;96:736-742 pubmed publisher
    ..Our previous study demonstrated that Rab3A, a small GTP-binding protein, is a new interacting partner of Syt I and could bind to both of the C2 domains; the ..
  6. Wollert T, Patel A, Lee Y, Provance D, Vought V, Cosgrove M, et al. Myosin5a tail associates directly with Rab3A-containing compartments in neurons. J Biol Chem. 2011;286:14352-61 pubmed publisher
    ..A potential class of binding partners are Rab GTPases and Rab3A is known to associate with SVs and is involved in SV trafficking...
  7. Ostermeier C, Brunger A. Structural basis of Rab effector specificity: crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A. Cell. 1999;96:363-74 pubmed
    The small G protein Rab3A plays an important role in the regulation of neurotransmitter release. The crystal structure of activated Rab3A/GTP/Mg2+ bound to the effector domain of rabphilin-3A was solved to 2.6 A resolution...
  8. Geppert M, Goda Y, Stevens C, Sudhof T. The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion. Nature. 1997;387:810-4 pubmed
    ..In brain, the Rab protein Rab3A is specific to synaptic vesicles, whose exocytosis can be monitored with submillisecond resolution by following ..
  9. Gerondopoulos A, Bastos R, Yoshimura S, Anderson R, Carpanini S, Aligianis I, et al. Rab18 and a Rab18 GEF complex are required for normal ER structure. J Cell Biol. 2014;205:707-20 pubmed publisher
    ..Micro syndrome is therefore a disease characterized by direct loss of Rab18 function or loss of Rab18 activation at the ER by its GEF Rab3GAP. ..
  10. Liang F, Zhang B, Tang J, Guo J, Li W, Ling E, et al. RIM3gamma is a postsynaptic protein in the rat central nervous system. J Comp Neurol. 2007;503:501-10 pubmed
    RIMs (Rab3-interacting molecules) are synaptic proteins essential for neural transmission and plasticity. RIM1alpha has been implicated in membrane trafficking and regulation of secretory vesicle exocytosis in eukaryotic cells...
  11. Handley M, Haynes L, Burgoyne R. Differential dynamics of Rab3A and Rab27A on secretory granules. J Cell Sci. 2007;120:973-84 pubmed
    We have assessed the dynamics of the association of Rab3A and Rab27A with secretory granules at various stages of their life in PC12 cells...
  12. Takamori S, Holt M, Stenius K, Lemke E, Gr Nborg M, Riedel D, et al. Molecular anatomy of a trafficking organelle. Cell. 2006;127:831-46 pubmed publisher
  13. Deak F, Shin O, Tang J, Hanson P, Ubach J, Jahn R, et al. Rabphilin regulates SNARE-dependent re-priming of synaptic vesicles for fusion. EMBO J. 2006;25:2856-66 pubmed
    ..is catalyzed by assembly of synaptic SNARE complexes, and is regulated by the synaptic vesicle GTP-binding protein Rab3 that binds to RIM and to rabphilin...
  14. Schl├╝ter O, Khvotchev M, Jahn R, Sudhof T. Localization versus function of Rab3 proteins. Evidence for a common regulatory role in controlling fusion. J Biol Chem. 2002;277:40919-29 pubmed
    b>Rab3A, Rab3B, Rab3C, and Rab3D constitute a family of GTP-binding proteins that are implicated in regulated exocytosis...
  15. Star E, Newton A, Murthy V. Real-time imaging of Rab3a and Rab5a reveals differential roles in presynaptic function. J Physiol. 2005;569:103-17 pubmed
    We investigated the roles of two Rab-family proteins, Rab3a and Rab5a, in hippocampal synaptic transmission using real-time fluorescence imaging...
  16. Alory C, Balch W. Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily. Mol Biol Cell. 2003;14:3857-67 pubmed
    ..We have now isolated REP mutant proteins that are able to partially function as both REP and GDI. These results provide molecular insight into the functional and evolutionary organization of the REP/GDI superfamily. ..
  17. Liu Y, Ren H, Wu C, Bai S, Zhang X, Ru B. Attenuation of zinc-induced neuronal death by the interaction of growth inhibitory factor with Rab3A in rat hippocampal neurons. Neurosci Lett. 2004;358:149-52 pubmed
    ..Neither the addition of recombinant GIF nor Rab3A alone could rescue these cells from death...
  18. Cussac D, Leblanc P, L Heritier A, Bertoglio J, Lang P, Kordon C, et al. Rho proteins are localized with different membrane compartments involved in vesicular trafficking in anterior pituitary cells. Mol Cell Endocrinol. 1996;119:195-206 pubmed
    ..b>Rab3 was localized like a fraction of synaptophysin, suggesting a role for rab3 in the targeting of "synaptic-like' ..
  19. Riedel D, Antonin W, Fernandez Chacon R, Alvarez de Toledo G, Jo T, Geppert M, et al. Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules. Mol Cell Biol. 2002;22:6487-97 pubmed
    Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland...
  20. Coppola T, Frantz C, Perret Menoud V, Gattesco S, Hirling H, Regazzi R. Pancreatic beta-cell protein granuphilin binds Rab3 and Munc-18 and controls exocytosis. Mol Biol Cell. 2002;13:1906-15 pubmed
    ..found to bind to two components of the secretory machinery of pancreatic beta-cells, the small GTP-binding protein Rab3 and the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-binding protein Munc-18...
  21. Dumas J, Zhu Z, Connolly J, Lambright D. Structural basis of activation and GTP hydrolysis in Rab proteins. Structure. 1999;7:413-23 pubmed
    ..The 2. 0 A crystal structure of GppNHp-bound Rab3A reveals the structural determinants that stabilize the active conformation and regulate GTPase activity...
  22. Maienschein V, Marxen M, Volknandt W, Zimmermann H. A plethora of presynaptic proteins associated with ATP-storing organelles in cultured astrocytes. Glia. 1999;26:233-44 pubmed
    ..organellar association of a large variety of synaptic proteins (synaptobrevin II, synaptotagmin I, synaptophysin, rab3a, synapsin I, SNAP-25, and syntaxin I) and also of the ubiquitous cellubrevin...
  23. Tabellini G, Baldini G, Bortul R, Bareggi R, Narducci P, Martelli A. Localization of the small monomeric GTPases Rab3D and Rab3A in the AtT-20 rat pituitary cell line. Eur J Histochem. 2001;45:347-56 pubmed
    We investigated the cellular localization of the small GTPases Rab3D and Rab3A in AtT-20 cells treated with the drug Brefeldin A...
  24. Luo H, Saiardi A, Nagata E, Ye K, Yu H, Jung T, et al. GRAB: a physiologic guanine nucleotide exchange factor for Rab3A, which interacts with inositol hexakisphosphate kinase. Neuron. 2001;31:439-51 pubmed
    ..the identification, cloning, and characterization of a novel protein, GRAB (guanine nucleotide exchange factor for Rab3A), which interacts with both InsP6K1 and Rab3A, a Ras-like GTPase that regulates synaptic vesicle exocytosis...
  25. Han S, Park D, Park S, Hong S. Identification of Rab6 as an N-ethylmaleimide-sensitive fusion protein-binding protein. Biochem J. 2000;352 Pt 1:165-73 pubmed
    ..Furthermore, we found that Rab3 and Rab4 can also associate with NSF and stimulate its ATPase activity...
  26. Dai Z, Tang X, Chen J, Tang X, Wang X. Rab3A Inhibition of Ca2+ -Dependent Dopamine Release From PC12 Cells Involves Interaction With Synaptotagmin I. J Cell Biochem. 2017;118:3696-3705 pubmed publisher
    ..Here, we studied the effects of Rab3A and synaptotagmin I (Syt I) on dopamine release using PC12 cells as a model system...
  27. Brondyk W, McKiernan C, Fortner K, Stabila P, Holz R, Macara I. Interaction cloning of Rabin3, a novel protein that associates with the Ras-like GTPase Rab3A. Mol Cell Biol. 1995;15:1137-43 pubmed
    b>Rab3A is a small, Ras-like GTPase expressed in neuroendocrine cells, in which it is associated with secretory vesicle membranes and regulates exocytosis...
  28. Huang C, Yang D, Lin C, Kao L. Involvement of Rab3A in vesicle priming during exocytosis: interaction with Munc13-1 and Munc18-1. Traffic. 2011;12:1356-70 pubmed publisher
    b>Rab3A is a small G-protein of the Rab family that is involved in the late steps of exocytosis. Here, we studied the role of Rab3A and its relationship with Munc13-1 and Munc18-1 during vesicle priming...
  29. Wang Y, Sugita S, Sudhof T. The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins. J Biol Chem. 2000;275:20033-44 pubmed
    ..RIM1 is localized close to the active zone at the synapse, where it interacts in a GTP-dependent manner with Rab3 located on synaptic vesicles...
  30. Pavlos N, Cheng T, Qin A, Ng P, Feng H, Ang E, et al. Tctex-1, a novel interaction partner of Rab3D, is required for osteoclastic bone resorption. Mol Cell Biol. 2011;31:1551-64 pubmed publisher
    ..Rab3D, a member of the Rab3 subfamily (Rab3A/B/C/D) of small exocytotic GTPases, represents a core component of the osteoclastic vesicle transport machinery...
  31. Clabecq A, Henry J, Darchen F. Biochemical characterization of Rab3-GTPase-activating protein reveals a mechanism similar to that of Ras-GAP. J Biol Chem. 2000;275:31786-91 pubmed
    ..We have characterized the activity of recombinant Rab3-GAP on Rab3A in solution. The K(m) and K(d) values (75 microm) indicate a low affinity of Rab3-GAP for its substrate...
  32. Tanaka M, Miyoshi J, Ishizaki H, Togawa A, Ohnishi K, Endo K, et al. Role of Rab3 GDP/GTP exchange protein in synaptic vesicle trafficking at the mouse neuromuscular junction. Mol Biol Cell. 2001;12:1421-30 pubmed
    The Rab3 small G protein family consists of four members, Rab3A, -3B, -3C, and -3D. Of these members, Rab3A regulates Ca(2+)-dependent neurotransmitter release...
  33. Rupnik M, Kreft M, Nothias F, Grilc S, Bobanovic L, Johannes L, et al. Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs. Am J Physiol Cell Physiol. 2007;292:C98-105 pubmed
    ..microscope-based analysis of immunocytochemistry to show that rat melanotrophs contain about equal amounts of Rab3A and Rab3B transcripts as well as proteins...
  34. Park J, Kim J, Lee J, Kim J, Seo J, Kim A. GTP binds to Rab3A in a complex with Ca2+/calmodulin. Biochem J. 2002;362:651-7 pubmed
    ..b>Rab3A is present as either the soluble or the SV membrane-bound form in neurons that are dependent on the GDP- or GTP-..
  35. Sakisaka T, Meerlo T, Matteson J, Plutner H, Balch W. Rab-alphaGDI activity is regulated by a Hsp90 chaperone complex. EMBO J. 2002;21:6125-35 pubmed
    ..is sensitive to the Hsp90 inhibitor geldanamycin (GA) and that GA inhibits the ability of alphaGDI to recycle Rab3A during neurotransmitter release...
  36. Rastaldi M, Armelloni S, Berra S, Li M, Pesaresi M, Poczewski H, et al. Glomerular podocytes possess the synaptic vesicle molecule Rab3A and its specific effector rabphilin-3a. Am J Pathol. 2003;163:889-99 pubmed
    ..In neurons, the small guanosine triphosphatase Rab3A and its effector rabphilin-3A form a complex required for the correct docking of synaptic vesicles to their target ..
  37. Pavlos N, Gr Nborg M, Riedel D, Chua J, Boyken J, Kloepper T, et al. Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. J Neurosci. 2010;30:13441-53 pubmed publisher
    ..iTRAQ) together with quantitative immunoblotting and fluorescence microscopy, we have determined the exocytotic (Rab3a, Rab3b, Rab3c, and Rab27b) and endocytic (Rab4b, Rab5a/b, Rab10, Rab11b, and Rab14) Rab machinery of SVs...
  38. Francis S, Sunshine C, Kirk K. Coordinate regulation of catecholamine uptake by rab3 and phosphoinositide 3-kinase. J Biol Chem. 2002;277:7816-23 pubmed
    ..b>rab3A and rab3B also increased NE uptake into large dense core vesicles in digitonin-permeabilized PC12 cells, which ..
  39. Lin C, Huang C, Lin K, Cheng K, Yang D, Tsai Y, et al. Visualization of Rab3A dissociation during exocytosis: a study by total internal reflection microscopy. J Cell Physiol. 2007;211:316-26 pubmed
    b>Rab3A is a small G protein in the Rab3 subfamily, and is thought to act at late stage of exocytosis. However, the detailed mechanism of its action is not completely understood...
  40. Koumanov F, Pereira V, Richardson J, Sargent S, Fazakerley D, Holman G. Insulin regulates Rab3-Noc2 complex dissociation to promote GLUT4 translocation in rat adipocytes. Diabetologia. 2015;58:1877-86 pubmed publisher
    ..Using constitutively active and dominant negative mutants and Rab3 knockdown we provide evidence that Rab3 isoforms are key regulators of GLUT4 translocation in adipocytes...
  41. Touchot N, Chardin P, Tavitian A. Four additional members of the ras gene superfamily isolated by an oligonucleotide strategy: molecular cloning of YPT-related cDNAs from a rat brain library. Proc Natl Acad Sci U S A. 1987;84:8210-4 pubmed
    ..rab1 is a mammalian homolog of the yeast YPT gene, and the four rab genes constitute an additional branch of the ras gene superfamily that to our knowledge has not been described in higher eukaryotes. ..