Rab27a

Summary

Gene Symbol: Rab27a
Description: RAB27A, member RAS oncogene family
Alias: ram, ras-related protein Rab-27A, GTP-binding protein Ram, low Mr GTP-binding protein, rab-27, ram p25
Species: rat
Products:     Rab27a

Top Publications

  1. Chen D, Guo J, Miki T, Tachibana M, Gahl W. Molecular cloning and characterization of rab27a and rab27b, novel human rab proteins shared by melanocytes and platelets. Biochem Mol Med. 1997;60:27-37 pubmed
    ..rab27b, from human melanoma cells and melanocytes, Rab27a is the human homolog of a rat megakaryocyte rab called ram p25. Rab27b corresponds to a small GTP-binding protein, c25KG, which was previously purified from platelets but whose ..
  2. Wilson S, Yip R, Swing D, O Sullivan T, Zhang Y, Novak E, et al. A mutation in Rab27a causes the vesicle transport defects observed in ashen mice. Proc Natl Acad Sci U S A. 2000;97:7933-8 pubmed
    ..Here, using positional cloning and bacterial artificial chromosome rescue, we show that ash encodes Rab27a. Rab GTPases represent the largest branch of the p21 Ras superfamily and are recognized as key players in ..
  3. Ostrowski M, Carmo N, Krumeich S, Fanget I, Raposo G, Savina A, et al. Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 2010;12:19-30; sup pp 1-13 pubmed publisher
    ..Among these, Rab27a and Rab27b were found to function in MVE docking at the plasma membrane...
  4. Nagata K, Satoh T, Itoh H, Kozasa T, Okano Y, Doi T, et al. The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library. FEBS Lett. 1990;275:29-32 pubmed
    ..The protein is designated as ram (ras-related gene from megakaryocyte) protein (ram p25)...
  5. Hume A, Collinson L, Rapak A, Gomes A, Hopkins C, Seabra M. Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J Cell Biol. 2001;152:795-808 pubmed
    Rab GTPases are regulators of intracellular membrane traffic. We report a possible function of Rab27a, a protein implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, ..
  6. Bahadoran P, Aberdam E, Mantoux F, Busca R, Bille K, Yalman N, et al. Rab27a: A key to melanosome transport in human melanocytes. J Cell Biol. 2001;152:843-50 pubmed
    ..However, it was demonstrated recently that GS can also be caused by a mutation in the Rab27a gene. These observations prompted us to investigate the role of Rab27a in melanosome transport...
  7. Strom M, Hume A, Tarafder A, Barkagianni E, Seabra M. A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport. J Biol Chem. 2002;277:25423-30 pubmed
    The Rab27a GTPase regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes, and lytic granule release in cytotoxic T lymphocytes...
  8. Nagata K, Suzuki T, Shibagaki Y, Mizumoto K, Okano Y, Kaziro Y, et al. Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli. J Biol Chem. 1992;267:19600-6 pubmed
    ..inserted into a bacterial expression vector; this allowed the production at a high level of soluble recombinant ram p25 in Escherichia coli. The purified ram p25 contained an equimolar amount of GDP...
  9. Westbroek W, Lambert J, De Schepper S, Kleta R, Van Den Bossche K, Seabra M, et al. Rab27b is up-regulated in human Griscelli syndrome type II melanocytes and linked to the actin cytoskeleton via exon F-Myosin Va transcripts. Pigment Cell Res. 2004;17:498-505 pubmed
    ..In most Griscelli patients, the RAB27A gene, which encodes a small GTPase that is associated with the melanosome membrane in melanocytes, is mutated...

More Information

Publications36

  1. Neeft M, Wieffer M, de Jong A, Negroiu G, Metz C, van Loon A, et al. Munc13-4 is an effector of rab27a and controls secretion of lysosomes in hematopoietic cells. Mol Biol Cell. 2005;16:731-41 pubmed
    ..The disease is caused by the absence of functional rab27a, but how rab27a controls secretion of lytic granule contents remains elusive...
  2. Kawakami T, He J, Morita H, Yokoyama K, Kaji H, Tanaka C, et al. Rab27a is essential for the formation of neutrophil extracellular traps (NETs) in neutrophil-like differentiated HL60 cells. PLoS ONE. 2014;9:e84704 pubmed publisher
    ..The Rab family small GTPase Rab27a is an important component of the secretory machinery of azurophilic granules in neutrophils...
  3. Zheng Y, Campbell E, Lucocq J, Riches A, Powis S. Monitoring the Rab27 associated exosome pathway using nanoparticle tracking analysis. Exp Cell Res. 2013;319:1706-13 pubmed publisher
    ..the inhibition of exosome secretion from MDA-MB-231 breast cancer cells expressing inhibitory RNA targeted for Rab27a, a known component of the exosome pathway...
  4. Ohbayashi N, Maruta Y, Ishida M, Fukuda M. Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes. J Cell Sci. 2012;125:1508-18 pubmed publisher
    ..Mreg deficiency was found to restore peripheral melanosome distribution from perinuclear melanosome aggregation in Rab27A-deficient melanocytes. However, the function of Mreg in melanosome transport has remained unclear...
  5. Ejlerskov P, Christensen D, Beyaie D, Burritt J, Paclet M, Gorlach A, et al. NADPH oxidase is internalized by clathrin-coated pits and localizes to a Rab27A/B GTPase-regulated secretory compartment in activated macrophages. J Biol Chem. 2012;287:4835-52 pubmed publisher
    ..CD40L as physiological inducers of cyt b(558) exocytosis to the cell surface, and by shRNA knockdown, we identify Rab27A/B as positive or negative regulators of vesicular mobilization to the phagosome or the cell surface, respectively...
  6. Wang Z, Thurmond D. Mechanisms of biphasic insulin-granule exocytosis - roles of the cytoskeleton, small GTPases and SNARE proteins. J Cell Sci. 2009;122:893-903 pubmed publisher
  7. Futter C, Ramalho J, Jaissle G, Seeliger M, Seabra M. The role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells. Mol Biol Cell. 2004;15:2264-75 pubmed
    ..distribution is due to the interaction with the cortical actin cytoskeleton mediated by a tripartite complex of Rab27a, melanophilin, and myosin Va...
  8. Haddad E, Wu X, Hammer J, Henkart P. Defective granule exocytosis in Rab27a-deficient lymphocytes from Ashen mice. J Cell Biol. 2001;152:835-42 pubmed
    Because mutations in Rab27a have been linked to immune defects in humans, we have examined cytotoxic lymphocyte function in ashen mice, which contain a splicing mutation in Rab27a...
  9. El Amraoui A, Schonn J, Küssel Andermann P, Blanchard S, Desnos C, Henry J, et al. MyRIP, a novel Rab effector, enables myosin VIIa recruitment to retinal melanosomes. EMBO Rep. 2002;3:463-70 pubmed
    ..protein that we have named MyRIP (myosin-VIIa- and Rab-interacting protein), since it also binds to Rab27A in a GTP-dependent manner...
  10. Imai A, Yoshie S, Nashida T, Shimomura H, Fukuda M. The small GTPase Rab27B regulates amylase release from rat parotid acinar cells. J Cell Sci. 2004;117:1945-53 pubmed
    ..We show that Rab27B, a closely related isoform of Rab27A that regulates lysosome-related granule exocytosis in cytotoxic T lymphocytes, is abundantly expressed on amylase-..
  11. Menasche G, Ménager M, Lefebvre J, Deutsch E, Athman R, Lambert N, et al. A newly identified isoform of Slp2a associates with Rab27a in cytotoxic T cells and participates to cytotoxic granule secretion. Blood. 2008;112:5052-62 pubmed publisher
    ..This process involves polarization of the CTL's secretory machinery and cytotoxic granules. The small GTPase Rab27a and the hMunc13-4 protein have been shown to be required for both granule maturation and granule docking and ..
  12. Bahadoran P, Busca R, Chiaverini C, Westbroek W, Lambert J, Bille K, et al. Characterization of the molecular defects in Rab27a, caused by RAB27A missense mutations found in patients with Griscelli syndrome. J Biol Chem. 2003;278:11386-92 pubmed
    b>Rab27a plays a pivotal role in the transport of melanosomes to dendrite tips of melanocytes and mutations in RAB27A, which impair melanosome transport cause the pigmentary dilution and the immune deficiency found in several patients with ..
  13. Imai A, Yoshie S, Nashida T, Shimomura H, Fukuda M. Functional involvement of Noc2, a Rab27 effector, in rat parotid acinar cells. Arch Biochem Biophys. 2006;455:127-35 pubmed
    ..Our results suggest that the Noc2/Rab27 complex is an important constituent of the early stages of IPR-stimulated amylase release. ..
  14. Ménager M, Menasche G, Romao M, Knapnougel P, Ho C, Garfa M, et al. Secretory cytotoxic granule maturation and exocytosis require the effector protein hMunc13-4. Nat Immunol. 2007;8:257-67 pubmed
    ..b>Rab27a and hMunc13-4 are critical effectors of the exocytosis of cytotoxic granules...
  15. Chavas L, Ihara K, Kawasaki M, Torii S, Uejima T, Kato R, et al. Elucidation of Rab27 recruitment by its effectors: structure of Rab27a bound to Exophilin4/Slp2-a. Structure. 2008;16:1468-77 pubmed publisher
    ..b>Rab27a regulates numerous exocytotic pathways, and its dysfunction causes the Griscelli syndrome human immunodeficiency...
  16. Kierszenbaum A, Tres L, Rivkin E, Kang Decker N, van Deursen J. The acroplaxome is the docking site of Golgi-derived myosin Va/Rab27a/b- containing proacrosomal vesicles in wild-type and Hrb mutant mouse spermatids. Biol Reprod. 2004;70:1400-10 pubmed
    ..We also show that the actin-based motor protein myosin Va and its receptor, Rab27a/b, known to be involved in vesicle transport, are present in the Golgi and Golgi-derived proacrosomal vesicles in ..
  17. Handley M, Burgoyne R. The Rab27 effector Rabphilin, unlike Granuphilin and Noc2, rapidly exchanges between secretory granules and cytosol in PC12 cells. Biochem Biophys Res Commun. 2008;373:275-81 pubmed publisher
  18. Imai A, Ishida M, Fukuda M, Nashida T, Shimomura H. MADD/DENN/Rab3GEP functions as a guanine nucleotide exchange factor for Rab27 during granule exocytosis of rat parotid acinar cells. Arch Biochem Biophys. 2013;536:31-7 pubmed publisher
    ..Our findings indicated that MADD functions as a GEF for Rab27 in parotid acinar cells and that its GEF activity for Rab27, i.e., GDP/GTP cycling, is required for IPR-induced amylase release. ..
  19. Booth A, Tarafder A, Hume A, Recchi C, Seabra M. A role for Na+,K+-ATPase ?1 in regulating Rab27a localisation on melanosomes. PLoS ONE. 2014;9:e102851 pubmed publisher
    ..Here we used Rab27a localisation onto melanosomes as a model to investigate Rab targeting...
  20. Handley M, Haynes L, Burgoyne R. Differential dynamics of Rab3A and Rab27A on secretory granules. J Cell Sci. 2007;120:973-84 pubmed
    We have assessed the dynamics of the association of Rab3A and Rab27A with secretory granules at various stages of their life in PC12 cells...
  21. Fukuda M, Kanno E, Yamamoto A. Rabphilin and Noc2 are recruited to dense-core vesicles through specific interaction with Rab27A in PC12 cells. J Biol Chem. 2004;279:13065-75 pubmed
    ..in the regulation of secretory vesicle exocytosis, however, recently both proteins have been shown to bind Rab27A in vitro in preference to Rab3A (Fukuda, M. (2003) J. Biol. Chem...
  22. Anikster Y, Huizing M, Anderson P, Fitzpatrick D, Klar A, Gross Kieselstein E, et al. Evidence that Griscelli syndrome with neurological involvement is caused by mutations in RAB27A, not MYO5A. Am J Hum Genet. 2002;71:407-14 pubmed
    ..Most patients with GS display the hemophagocytic syndrome and have mutations in RAB27A, which codes for a small GTPase...
  23. Zografou S, Basagiannis D, Papafotika A, Shirakawa R, Horiuchi H, Auerbach D, et al. A complete Rab screening reveals novel insights in Weibel-Palade body exocytosis. J Cell Sci. 2012;125:4780-90 pubmed publisher
    ..Yet, the only known WPB-associated Rabs are Rab27a and Rab3d...
  24. Wu X, Rao K, Zhang H, Wang F, Sellers J, Matesic L, et al. Identification of an organelle receptor for myosin-Va. Nat Cell Biol. 2002;4:271-8 pubmed
    ..motor, binds to one of its cargoes, the melanosome, by interacting with a receptor-protein complex containing Rab27a and melanophilin, a postulated Rab27a effector...
  25. Kondo H, Shirakawa R, Higashi T, Kawato M, Fukuda M, Kita T, et al. Constitutive GDP/GTP exchange and secretion-dependent GTP hydrolysis activity for Rab27 in platelets. J Biol Chem. 2006;281:28657-65 pubmed
    ..Taken together, Rab27 is maintained in the active status in unstimulated platelets, which could function to keep dense granules in a preparative status for secretion. ..
  26. Gibbs D, Azarian S, Lillo C, Kitamoto J, Klomp A, Steel K, et al. Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes. J Cell Sci. 2004;117:6473-83 pubmed
    ..in RPE cells that could potentially participate in melanosome transport, but of those tested, only myosin VIIa and Rab27a were found to be required for normal distribution...
  27. Goishi K, Mizuno K, Nakanishi H, Sasaki T. Involvement of Rab27 in antigen-induced histamine release from rat basophilic leukemia 2H3 cells. Biochem Biophys Res Commun. 2004;324:294-301 pubmed
    ..Recent studies indicate that one member of the Rab family, Rab27A, regulates the transport of lysosome-related organelles, such as melanosome distribution in melanocytes, lytic ..