Pxn

Summary

Gene Symbol: Pxn
Description: paxillin
Alias: paxillin, myocardial ischemic preconditioning associated protein 7
Species: rat
Products:     Pxn

Top Publications

  1. Nikolopoulos S, Turner C. Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. J Biol Chem. 2001;276:23499-505 pubmed
    b>Paxillin is a focal adhesion adapter protein involved in integrin signaling...
  2. Hagel M, George E, Kim A, Tamimi R, Opitz S, Turner C, et al. The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling. Mol Cell Biol. 2002;22:901-15 pubmed
    ..We have examined the role of one of the best-characterized integrin effectors, the focal adhesion protein paxillin, by disruption of the paxillin gene in mice...
  3. Spiczka K, Yeaman C. Ral-regulated interaction between Sec5 and paxillin targets Exocyst to focal complexes during cell migration. J Cell Sci. 2008;121:2880-91 pubmed publisher
    ..targeting of Exocyst-dependent cargo, is dependent on Ral GTPases, which control association between Sec5 and paxillin. Overexpression of Ral-uncoupled Sec5 mutants inhibited Exocyst interaction with paxillin in 5'A cells, as did ..
  4. Ishibe S, Joly D, Zhu X, Cantley L. Phosphorylation-dependent paxillin-ERK association mediates hepatocyte growth factor-stimulated epithelial morphogenesis. Mol Cell. 2003;12:1275-85 pubmed
    ..by the tyrosine phosphorylation-dependent association of inactive ERK and the focal adhesion complex protein paxillin. In addition, paxillin was found to associate with the upstream MAP kinases Raf and MEK, resulting in a complex ..
  5. Ohanian V, Gatfield K, Ohanian J. Role of the actin cytoskeleton in G-protein-coupled receptor activation of PYK2 and paxillin in vascular smooth muscle. Hypertension. 2005;46:93-9 pubmed
    ..Tyrosine phosphorylation of the adaptor protein paxillin has been implicated in regulation of actin filament formation and force development...
  6. Chen Y, Yi B, Wang Z, Gu J, Li Y, Cui J, et al. Paxillin suppresses the proliferation of HPS rat serum treated PASMCs by up-regulating the expression of cytoskeletal proteins. Mol Biosyst. 2014;10:759-66 pubmed publisher
    ..In addition, it has been identified that paxillin could influence the cytoskeletal protein expression by some important signaling pathways in many diseases, ..
  7. Miyamoto Y, Yamauchi J, Chan J, Okada A, Tomooka Y, Hisanaga S, et al. Cdk5 regulates differentiation of oligodendrocyte precursor cells through the direct phosphorylation of paxillin. J Cell Sci. 2007;120:4355-66 pubmed
    ..We also show that the focal adhesion protein paxillin is directly phosphorylated at Ser244 by Cdk5...
  8. Melendez J, Turner C, Avraham H, Steinberg S, Schaefer E, Sussman M. Cardiomyocyte apoptosis triggered by RAFTK/pyk2 via Src kinase is antagonized by paxillin. J Biol Chem. 2004;279:53516-23 pubmed
    ..phenotypic characteristics are prevented by concurrent expression of wild-type or a phosphorylation-deficient paxillin mutated at Tyr(31) and Tyr(118)...
  9. Bienvenu G, Seurin D, Le Bouc Y, Even P, Babajko S, Magnan C. Dysregulation of energy homeostasis in mice overexpressing insulin-like growth factor-binding protein 6 in the brain. Diabetologia. 2005;48:1189-97 pubmed
    ..These transgenic h-IGFBP-6 mice may be considered a new tool for studies of the involvement of the brain IGF system in metabolism control and obesity. ..

More Information

Publications46

  1. Kaneko Y, Lindsay L, Murphy C. Focal adhesions disassemble during early pregnancy in rat uterine epithelial cells. Reprod Fertil Dev. 2008;20:892-9 pubmed
    ..The present study investigated the distribution and expression of two focal adhesion proteins, namely talin and paxillin, in rat uterine epithelial cells during early pregnancy and their role in the loss of these cells at the time of ..
  2. Cohen Hillel E, Mintz R, Meshel T, Garty B, Ben Baruch A. Cell migration to the chemokine CXCL8: paxillin is activated and regulates adhesion and cell motility. Cell Mol Life Sci. 2009;66:884-99 pubmed publisher
    ..In this study, we found that CXCL8 stimulation led to paxillin phosphorylation in normal neutrophils, and that both CXCL8 receptors (CXCR1 and CXCR2) mediated CXCL8-induced ..
  3. Legate K, Montanez E, Kudlacek O, Fassler R. ILK, PINCH and parvin: the tIPP of integrin signalling. Nat Rev Mol Cell Biol. 2006;7:20-31 pubmed
    ..All these proteins have synergistic functions at focal adhesions, but recent work has indicated that these proteins might also have separate roles within a cell. They function as regulators of gene transcription or cell-cell adhesion. ..
  4. Bianchi Smiraglia A, Kunnev D, Limoge M, Lee A, Beckerle M, Bakin A. Integrin-?5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor ?. Cell Cycle. 2013;12:3377-89 pubmed publisher
    ..These findings provide a platform for defining the molecular mechanism regulating the organization and activities of VSFs in response to TGF-?. ..
  5. Andreolotti A, Bragado M, Tapia J, Jensen R, Garcia Marin L. Cholecystokinin rapidly stimulates CrkII function in vivo in rat pancreatic acini. Formation of CrkII-protein complexes. Eur J Biochem. 2003;270:4706-13 pubmed
    ..We demonstrated that CCK promoted the transient formation of CrkII-paxillin and CrkII-p130Cas complexes with maximal effect at 1 min...
  6. Vogelezang M, Forster U, Han J, Ginsberg M, Ffrench Constant C. Neurite outgrowth on a fibronectin isoform expressed during peripheral nerve regeneration is mediated by the interaction of paxillin with alpha4beta1 integrins. BMC Neurosci. 2007;8:44 pubmed
    ..Here we use PC12 cells to examine the role of the interaction between paxillin and the alpha4 integrin cytoplasmic domain in neurite outgrowth...
  7. Rafiq K, Guo J, Vlasenko L, Guo X, Kolpakov M, Sanjay A, et al. c-Cbl ubiquitin ligase regulates focal adhesion protein turnover and myofibril degeneration induced by neutrophil protease cathepsin G. J Biol Chem. 2012;287:5327-39 pubmed publisher
    ..G promotes c-Cbl interaction with FA proteins, resulting in enhanced c-Cbl-mediated FA protein ubiquitination and degradation, myofibril degradation, and subsequent down-regulation of myocyte survival signaling. ..
  8. Lamorte L, Kamikura D, Park M. A switch from p130Cas/Crk to Gab1/Crk signaling correlates with anchorage independent growth and JNK activation in cells transformed by the Met receptor oncoprotein. Oncogene. 2000;19:5973-81 pubmed
    ..In contrast to control cells, focal adhesion kinase, p130Cas and paxillin are weakly or not detectably tyrosine phosphorylated in Met transformed cells...
  9. Senechal K, Heaney C, Druker B, Sawyers C. Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells. Mol Cell Biol. 1998;18:5082-90 pubmed
    ..When phosphorylated, Crkl binds through its Src homology 2 (SH2) domain to other tyrosine phosphoproteins such as paxillin and Cbl. Overexpression of Crkl in fibroblasts induces transformation...
  10. Gorostizaga A, Mori Sequeiros Garcia M, Acquier A, López Costa J, Mendez C, Maloberti P, et al. ACTH Modulates PTP-PEST Activity and Promotes Its Interaction With Paxillin. J Cell Biochem. 2016;117:2170-81 pubmed publisher
    Adrenocorticotropic hormone (ACTH) treatment has been proven to promote paxillin dephosphorylation and increase soluble protein tyrosine phosphatase (PTP) activity in rat adrenal zona fasciculata (ZF)...
  11. Kaneko Y, Lecce L, Murphy C. Ovarian hormones regulate expression of the focal adhesion proteins, talin and paxillin, in rat uterine luminal but not glandular epithelial cells. Histochem Cell Biol. 2009;132:613-22 pubmed publisher
    ..effect of ovarian hormones on the distribution and protein expression of two focal adhesion proteins, talin and paxillin, in rat uterine luminal and glandular epithelial cells under various hormone regimes...
  12. Rice K, Desai D, Kinnard R, Harris R, Wright G, Blough E. Load-induced focal adhesion mechanotransduction is altered with aging in the Fischer 344/NNiaHSd x Brown Norway/BiNia rat aorta. Biogerontology. 2007;8:257-67 pubmed
    ..increases in the aortic content of FAK (15%), FAK related non-kinase (p41-FRNK) (28%), Src (92%), RhoA (41%), and paxillin (23%) in the very aged aortae...
  13. Sorenson C. Interaction of bcl-2 with Paxillin through its BH4 domain is important during ureteric bud branching. J Biol Chem. 2004;279:11368-74 pubmed
    ..and/or may play a more "active" role during morphogenesis by interacting with other proteins such as paxillin. Recent work in this laboratory demonstrated that bcl-2 directly associates with paxillin...
  14. Houle F, Rousseau S, Morrice N, Luc M, Mongrain S, Turner C, et al. Extracellular signal-regulated kinase mediates phosphorylation of tropomyosin-1 to promote cytoskeleton remodeling in response to oxidative stress: impact on membrane blebbing. Mol Biol Cell. 2003;14:1418-32 pubmed
    ..of oxidative stress, in a misassembly of focal adhesions characterized by mislocalization of key proteins such as paxillin. The focal adhesion misassembly that followed ERK inhibition with the mitogen-activated protein kinase kinase (..
  15. Raimondi C, Fantin A, Lampropoulou A, Denti L, Chikh A, Ruhrberg C. Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. J Exp Med. 2014;211:1167-83 pubmed publisher
    ..integrin ligand fibronectin (FN) stimulated actin remodeling and phosphorylation of the focal adhesion component paxillin (PXN) in a VEGF/VEGFR2-independent but NRP1-dependent manner...
  16. Pratt S, Epple H, Ward M, Feng Y, Braga V, Longmore G. The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration. J Cell Biol. 2005;168:813-24 pubmed
  17. Vallés A, Beuvin M, Boyer B. Activation of Rac1 by paxillin-Crk-DOCK180 signaling complex is antagonized by Rap1 in migrating NBT-II cells. J Biol Chem. 2004;279:44490-6 pubmed
    ..Random motility of NBT-II carcinoma cells on collagen critically depends on paxillin phosphorylation at Tyr-31 and Tyr-118, the binding sites for the adapter protein CrkII...
  18. Ryu H, Lee J, Kim K, Jeong S, Kim P, Chung H. Regulation of neutrophil adhesion by pituitary growth hormone accompanies tyrosine phosphorylation of Jak2, p125FAK, and paxillin. J Immunol. 2000;165:2116-23 pubmed
    ..resulted in the morphological changes and the tyrosine phosphorylation of focal adhesion kinase (p125FAK) and paxillin. Preincubation with genistein, a tyrosine kinase inhibitor, blocked the GH-stimulated adhesion and Jak2, STAT3, ..
  19. Moik D, Böttcher A, Makhina T, Grashoff C, Bulus N, Zent R, et al. Mutations in the paxillin-binding site of integrin-linked kinase (ILK) destabilize the pseudokinase domain and cause embryonic lethality in mice. J Biol Chem. 2013;288:18863-71 pubmed publisher
    ..ILK in which Val(386) and Thr(387) were substituted with glycine residues (ILK-VT/GG) could neither interact with paxillin nor localize to FA in cells expressing endogenous wild-type ILK, implying that paxillin binding to ILK is ..
  20. Speert D, Konkle A, Zup S, Schwarz J, Shiroor C, Taylor M, et al. Focal adhesion kinase and paxillin: novel regulators of brain sexual differentiation?. Endocrinology. 2007;148:3391-401 pubmed
    ..Focal adhesion kinase (FAK) and paxillin, both members of the focal adhesion complex family of proteins, were significantly elevated in the newborn female ..
  21. Birukova A, Malyukova I, Poroyko V, Birukov K. Paxillin-beta-catenin interactions are involved in Rac/Cdc42-mediated endothelial barrier-protective response to oxidized phospholipids. Am J Physiol Lung Cell Mol Physiol. 2007;293:L199-211 pubmed
    ..assays have shown that OxPAPC induced translocation and peripheral accumulation of FA complexes containing paxillin, focal adhesion kinase, vinculin, GIT1, and GIT2, increased association of AJ proteins vascular endothelial-..
  22. Acevedo K, Moussi N, Li R, Soo P, Bernard O. LIM kinase 2 is widely expressed in all tissues. J Histochem Cytochem. 2006;54:487-501 pubmed
    ..We have also demonstrated that endogenous LIMK1 and LIMK2 form heterodimers, and that LIMK2 does not always interact with the same proteins as LIMK1. ..
  23. Guo J, Liu L, Yuan L, Wang N, De W. Expression and localization of paxillin in rat pancreas during development. World J Gastroenterol. 2011;17:4479-87 pubmed publisher
    To investigate the expression and localization of paxillin in rat pancreas during development. Pancreata from Sprague Dawley rat fetuses, embryos, young animals, and adult animals were used in this study...
  24. Peng X, Cuff L, Lawton C, DeMali K. Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin. J Cell Sci. 2010;123:567-77 pubmed publisher
    ..Thus, our study identifies vinculin as a novel regulator of E-cadherin function and provides important new insight into the dynamic regulation of adherens junctions. ..
  25. Chun S, Rasband M, Sidman R, Habib A, Vartanian T. Integrin-linked kinase is required for laminin-2-induced oligodendrocyte cell spreading and CNS myelination. J Cell Biol. 2003;163:397-408 pubmed
    ..A dominant negative ILK inhibits LN-2-induced myelinlike membrane formation. A critical component of the myelination signaling cascade includes LN-2 and integrin signals through ILK. ..
  26. Ishiki M, Sasaoka T, Ishihara H, Imamura T, Usui I, Takata Y, et al. Evidence for functional roles of Crk-II in insulin and epidermal growth factor signaling in Rat-1 fibroblasts overexpressing insulin receptors. Endocrinology. 1997;138:4950-8 pubmed
    ..Crk is an SH2 and SH3 domain-containing adaptor protein that has been reported to associate with p130cas, paxillin, c-cbl, c-abl, Sos, and C3G in vitro...
  27. Chen Y, Wen X, Wang Z, Zeng J, Chen B, Yang Y, et al. Annexin A2 inhibits the migration of PASMCs stimulated with HPS rat serum by down-regulating the expression of paxillin. Biochem Biophys Res Commun. 2016;469:70-75 pubmed publisher
    ..Annexin A2 (ANXA2) plays crucial role in HPS-associated PVR and might activate the activity of paxillin which as a regulatory protein participates in the regulation of PASMCs function in PVR...
  28. Lai K, Pawson T. The ShcA phosphotyrosine docking protein sensitizes cardiovascular signaling in the mouse embryo. Genes Dev. 2000;14:1132-45 pubmed
    ..ShcA may therefore orchestrate complex interactions within the vascular compartment by rendering cells permissive to respond to soluble and adhesive external cues. ..
  29. Rosse C, Formstecher E, Boeckeler K, Zhao Y, Kremerskothen J, White M, et al. An aPKC-exocyst complex controls paxillin phosphorylation and migration through localised JNK1 activation. PLoS Biol. 2009;7:e1000235 pubmed publisher
    ..The migration associated control of JNK by aPKCs determines JNK phosphorylation of the plasma membrane substrate Paxillin, but not the phosphorylation of the nuclear JNK substrate, c-jun...
  30. Torres A, Vasudevan L, Holowka D, Baird B. Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays. Proc Natl Acad Sci U S A. 2008;105:17238-44 pubmed publisher
    ..do not detectably concentrate with the array of clustered IgE-Fc epsilonRI, focal adhesion proteins vinculin, paxillin, and talin, which are known to link F-actin with integrins, accumulate in these regions on the same time scale as ..
  31. van der Flier A, Kuikman I, Kramer D, Geerts D, Kreft M, Takafuta T, et al. Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin [beta] subunits. J Cell Biol. 2002;156:361-76 pubmed
    ..These findings suggest that specific combinations of filamin mRNA splicing events modulate the organization of the actin cytoskeleton and the binding affinity for integrins. ..
  32. Nayal A, Webb D, Brown C, Schaefer E, Vicente Manzanares M, Horwitz A. Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. J Cell Biol. 2006;173:587-9 pubmed
    ..We show that p21-activated kinase (PAK)-induced phosphorylation of serine 273 in paxillin is a critical regulator of this turnover...
  33. Guan S, Chen M, Woodley D, Li W. Nckbeta adapter controls neuritogenesis by maintaining the cellular paxillin level. Mol Cell Biol. 2007;27:6001-11 pubmed
    ..for changes among the components reportedly present in complex with Nck, we found that the steady-state level of paxillin was significantly reduced in Nckbeta knockdown, but not Nckalpha knockdown, neurons...
  34. Ceccarelli S, Cardinali G, Aspite N, Picardo M, Marchese C, Torrisi M, et al. Cortactin involvement in the keratinocyte growth factor and fibroblast growth factor 10 promotion of migration and cortical actin assembly in human keratinocytes. Exp Cell Res. 2007;313:1758-77 pubmed
    ..Furthermore, siRNA-mediated downregulation of cortactin inhibited KGF- and FGF10-induced migration. These results indicate that cortactin is involved in keratinocyte migration promoted by both KGF and FGF10. ..
  35. Mazaki Y, Hashimoto S, Sabe H. Monocyte cells and cancer cells express novel paxillin isoforms with different binding properties to focal adhesion proteins. J Biol Chem. 1997;272:7437-44 pubmed
    ..Among them, paxillin is one of the important molecules interacting with a variety of signaling molecules and cytoskeletal building ..
  36. Cerna J, Cerecedo D, Ortega A, Garcia Sierra F, Centeno F, Garrido E, et al. Dystrophin Dp71f associates with the beta1-integrin adhesion complex to modulate PC12 cell adhesion. J Mol Biol. 2006;362:954-65 pubmed
    ..assays demonstrated that the PC12 cell beta1-integrin adhesion complex is composed of beta1-integrin, talin, paxillin, alpha-actinin, FAK and actin...
  37. Cariaga Martínez A, Cortés I, García E, Pérez García V, Pajares M, Idoate M, et al. Phosphoinositide 3-kinase p85beta regulates invadopodium formation. Biol Open. 2014;3:924-36 pubmed publisher
    ..These results show that p85β enhances invasion by inducing cell adhesion development into invadopodia-like structures explaining the metastatic potential of tumors with increased p85β levels. ..