Gene Symbol: Ptprj
Description: protein tyrosine phosphatase, receptor type, J
Alias: DEP-1, receptor-type tyrosine-protein phosphatase eta, density enhanced phosphatase-1
Species: rat
Products:     Ptprj

Top Publications

  1. Tangye S, Wu J, Aversa G, de Vries J, Lanier L, Phillips J. Negative regulation of human T cell activation by the receptor-type protein tyrosine phosphatase CD148. J Immunol. 1998;161:3803-7 pubmed
    ..Taken together, these results suggest that CD148 is an important phosphatase involved in negatively regulating the proximal signaling events during activation of Ag-specific T cells. ..
  2. Kovalenko M, Denner K, Sandstrom J, Persson C, Gross S, Jandt E, et al. Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem. 2000;275:16219-26 pubmed
    ..Our study demonstrates that DEP-1 dephosphorylation of the PDGF beta-receptor is site-selective and may lead to modulation, rather than general attenuation, of signaling. ..
  3. Baker J, Majeti R, Tangye S, Weiss A. Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation. Mol Cell Biol. 2001;21:2393-403 pubmed
    ..Based on these results, we propose that CD148 negatively regulates TCR signaling by interfering with the phosphorylation and function of PLCgamma1 and LAT. ..
  4. Sorby M, Sandstrom J, Ostman A. An extracellular ligand increases the specific activity of the receptor-like protein tyrosine phosphatase DEP-1. Oncogene. 2001;20:5219-24 pubmed
    ..These experiments identify Matrigel as a source of DEP-1 agonist(s) and provide the first evidence for upregulation of the specific activity of receptor-like protein tyrosine phosphatases by extracellular ligands. ..
  5. Palka H, Park M, Tonks N. Hepatocyte growth factor receptor tyrosine kinase met is a substrate of the receptor protein-tyrosine phosphatase DEP-1. J Biol Chem. 2003;278:5728-35 pubmed
  6. Lin J, Weiss A. The tyrosine phosphatase CD148 is excluded from the immunologic synapse and down-regulates prolonged T cell signaling. J Cell Biol. 2003;162:673-82 pubmed
    ..Upon T cell-APC disengagement, CD148 can then access and dephosphorylate substrates to down-regulate prolongation of signaling. ..
  7. Pera I, Iuliano R, Florio T, Susini C, Trapasso F, Santoro M, et al. The rat tyrosine phosphatase eta increases cell adhesion by activating c-Src through dephosphorylation of its inhibitory phosphotyrosine residue. Oncogene. 2005;24:3187-95 pubmed
    ..Interestingly, the extent of both c-Src dephosphorylation at Tyr 529, FAK and paxillin phosphorylation, and the increased cell adhesion were associated with the degree of r-PTPeta expression. ..
  8. Tsuboi N, Utsunomiya T, Roberts R, Ito H, Takahashi K, Noda M, et al. The tyrosine phosphatase CD148 interacts with the p85 regulatory subunit of phosphoinositide 3-kinase. Biochem J. 2008;413:193-200 pubmed publisher
    ..Taken together, these results demonstrate that CD148 may interact with and dephosphorylate p85 when it is phosphorylated and modulate the magnitude of PI3K activity. ..
  9. Holsinger L, Ward K, Duffield B, Zachwieja J, Jallal B. The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn). Oncogene. 2002;21:7067-76 pubmed
    ..p120(ctn) was found to colocalize with these structures. Together these data suggest an important role for DEP-1 in the function of cell-cell contacts and adherens junctions. ..

More Information


  1. Takahashi T, Takahashi K, St John P, Fleming P, Tomemori T, Watanabe T, et al. A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development. Mol Cell Biol. 2003;23:1817-31 pubmed
    ..These findings implicate a member of the receptor tyrosine phosphatase family, CD148, in developmental vascular organization and provide evidence that it regulates endothelial proliferation and endothelium-pericyte interactions. ..
  2. Citri A, Yarden Y. EGF-ERBB signalling: towards the systems level. Nat Rev Mol Cell Biol. 2006;7:505-16 pubmed
    ..Because network fragility is an inevitable trade-off of robustness, systems-level understanding is expected to generate therapeutic opportunities to intercept aberrant network activation. ..
  3. Balavenkatraman K, Jandt E, Friedrich K, Kautenburger T, Pool Zobel B, Ostman A, et al. DEP-1 protein tyrosine phosphatase inhibits proliferation and migration of colon carcinoma cells and is upregulated by protective nutrients. Oncogene. 2006;25:6319-24 pubmed
    ..Upregulation of DEP-1 expression, and in turn inhibition of cell growth and migration may present a previously unrecognized mechanism of chemoprevention by nutrients. ..
  4. Dave R, Hume D, Elsegood C, Kellie S. CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages. Exp Cell Res. 2009;315:1734-44 pubmed publisher
    ..There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity. ..
  5. Chabot C, Spring K, Gratton J, Elchebly M, Royal I. New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival. Mol Cell Biol. 2009;29:241-53 pubmed publisher
    ..Overall, our results reveal that despite its negative role on global VEGFR2 phosphorylation, DEP-1 is a positive regulator of VEGF-mediated Src and Akt activation and endothelial cell survival. ..
  6. Chin C, Sachs J, Engelman D. Transmembrane homodimerization of receptor-like protein tyrosine phosphatases. FEBS Lett. 2005;579:3855-8 pubmed
    ..Through a mutational study of the DEP1 TMD, we demonstrate that these interactions are specific. Taken together, our results define a subset of the RPTP family in which TM homodimerization may act as a mediator of protein function. ..
  7. Borges L, Seifert R, Grant F, Hart C, Disteche C, Edelhoff S, et al. Cloning and characterization of rat density-enhanced phosphatase-1, a protein tyrosine phosphatase expressed by vascular cells. Circ Res. 1996;79:570-80 pubmed
    ..The deduced structure and the regulation of expression of rDEP-1 suggest that it may play a role in adhesion and/or signaling events involving cell-cell and cell-matrix contact. ..
  8. Sallee J, Burridge K. Density-enhanced phosphatase 1 regulates phosphorylation of tight junction proteins and enhances barrier function of epithelial cells. J Biol Chem. 2009;284:14997-5006 pubmed publisher
    ..These data suggest that DEP-1 can modify the phosphorylation state of tight junction proteins and play a role in regulating permeability. ..
  9. Sacco F, Tinti M, Palma A, Ferrari E, Nardozza A, Hooft van Huijsduijnen R, et al. Tumor suppressor density-enhanced phosphatase-1 (DEP-1) inhibits the RAS pathway by direct dephosphorylation of ERK1/2 kinases. J Biol Chem. 2009;284:22048-58 pubmed publisher
    ..Overall this study provides novel insights into the anti-proliferative role of this phosphatase and proposes a new mechanism that may also be relevant for the regulation of density-dependent growth inhibition. ..
  10. Jandt E, Denner K, Kovalenko M, Ostman A, Bohmer F. The protein-tyrosine phosphatase DEP-1 modulates growth factor-stimulated cell migration and cell-matrix adhesion. Oncogene. 2003;22:4175-85 pubmed
    ..We propose that negative regulation of growth-factor stimulated cell migration and promotion of cell-matrix adhesion may be related to the function of DEP-1 as tumor suppressor. ..
  11. Grazia Lampugnani M, Zanetti A, Corada M, Takahashi T, Balconi G, Breviario F, et al. Contact inhibition of VEGF-induced proliferation requires vascular endothelial cadherin, beta-catenin, and the phosphatase DEP-1/CD148. J Cell Biol. 2003;161:793-804 pubmed
    ..In sparse cells or in VE-cadherin-null cells, this phenomenon cannot occur and the receptor is fully activated by the growth factor. ..
  12. Persson C, Engstrom U, Mowbray S, Ostman A. Primary sequence determinants responsible for site-selective dephosphorylation of the PDGF beta-receptor by the receptor-like protein tyrosine phosphatase DEP-1. FEBS Lett. 2002;517:27-31 pubmed
    ..DEP-1 site-selective dephosphorylation of PDGF beta-receptor is thus determined by the primary sequence surrounding phosphorylation sites and involves interactions with residues spanning at least between positions -1 and +3. ..
  13. Tarcic G, Boguslavsky S, Wakim J, Kiuchi T, Liu A, Reinitz F, et al. An unbiased screen identifies DEP-1 tumor suppressor as a phosphatase controlling EGFR endocytosis. Curr Biol. 2009;19:1788-98 pubmed publisher
    ..We report the identification of PTPRK and PTPRJ (density-enhanced phosphatase-1 [DEP-1]) as EGFR-targeting phosphatases...
  14. Omerovic J, Clague M, Prior I. Phosphatome profiling reveals PTPN2, PTPRJ and PTEN as potent negative regulators of PKB/Akt activation in Ras-mutated cancer cells. Biochem J. 2010;426:65-72 pubmed publisher
    ..with similar potencies including PTPN2 (T-cell protein tyrosine phosphatase; also known as TC-PTP) and PTPRJ (protein tyrosine phosphatase receptor type J; also known as DEP-1/CD148)...
  15. Gobert R, Joubert L, Curchod M, Salvat C, Foucault I, Jorand Lebrun C, et al. Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits. Mol Cell Biol. 2009;29:1538-53 pubmed publisher
    ..Oligodendrocytic differentiation feedback loops may therefore yield pharmacological targets to treat disease related to dysfunctional myelin deposition. ..
  16. Massa A, Barbieri F, Aiello C, Arena S, Pattarozzi A, Pirani P, et al. The expression of the phosphotyrosine phosphatase DEP-1/PTPeta dictates the responsivity of glioma cells to somatostatin inhibition of cell proliferation. J Biol Chem. 2004;279:29004-12 pubmed
    ..In conclusion we propose that the expression and activation of DEP-1/PTPeta is required for somatostatin inhibition of glioma proliferation. ..
  17. Arena S, Pattarozzi A, Massa A, Esteve J, Iuliano R, Fusco A, et al. An intracellular multi-effector complex mediates somatostatin receptor 1 activation of phospho-tyrosine phosphatase eta. Mol Endocrinol. 2007;21:229-46 pubmed
    ..In particular the sequential activation of JAK2, SHP-2, and Src conveys the molecular signaling from SSTR1 to the activation of this phosphatase that is responsible for the final biological effects of SST. ..
  18. Iervolino A, Iuliano R, Trapasso F, Viglietto G, Melillo R, Carlomagno F, et al. The receptor-type protein tyrosine phosphatase J antagonizes the biochemical and biological effects of RET-derived oncoproteins. Cancer Res. 2006;66:6280-7 pubmed
    ..We showed previously that the expression of the receptor-type protein tyrosine phosphatase J (PTPRJ) is suppressed in neoplastically transformed follicular thyroid cells...
  19. Kellie S, Craggs G, Bird I, Jones G. The tyrosine phosphatase DEP-1 induces cytoskeletal rearrangements, aberrant cell-substratum interactions and a reduction in cell proliferation. J Cell Sci. 2004;117:609-18 pubmed
    ..These results demonstrate that DEP-1 is a negative regulator of cell proliferation, cell-substratum contacts, motility and chemotaxis in fibroblasts. ..
  20. Singh D, Lampe P. Identification of connexin-43 interacting proteins. Cell Commun Adhes. 2003;10:215-20 pubmed
    ..Further in vitro/in vivo analysis of these interacting proteins will help in our understanding of the global role of connexins in regulating development, cell metabolism and growth. ..
  21. Petermann A, Haase D, Wetzel A, Balavenkatraman K, Tenev T, Gührs K, et al. Loss of the protein-tyrosine phosphatase DEP-1/PTPRJ drives meningioma cell motility. Brain Pathol. 2011;21:405-18 pubmed publisher
    DEP-1/PTPRJ is a transmembrane protein-tyrosine phosphatase which has been proposed as a suppressor of epithelial tumors...
  22. Hinojos C, Boerwinkle E, Fornage M, Doris P. Combined genealogical, mapping, and expression approaches to identify spontaneously hypertensive rat hypertension candidate genes. Hypertension. 2005;45:698-704 pubmed
    ..The present approach identifies a number of genes that may influence blood pressure in SHR by virtue of allelic effects on gene expression. ..
  23. Senis Y, Tomlinson M, Ellison S, Mazharian A, Lim J, Zhao Y, et al. The tyrosine phosphatase CD148 is an essential positive regulator of platelet activation and thrombosis. Blood. 2009;113:4942-54 pubmed publisher
    ..G protein-coupled receptor responses to thrombin and other agonists were also marginally reduced. These results highlight CD148 as a global regulator of platelet activation and a novel antithrombotic drug target. ..
  24. de la Fuente García M, Nicolas J, Freed J, Palou E, Thomas A, Vilella R, et al. CD148 is a membrane protein tyrosine phosphatase present in all hematopoietic lineages and is involved in signal transduction on lymphocytes. Blood. 1998;91:2800-9 pubmed
    ..In conclusion, the data presented show that CD148 corresponds to a previously described protein tyrosine phosphatase HPTP-eta/DEP-1 and that this molecule is involved in signal transduction in lymphocytes. ..