Gene Symbol: Prkar1a
Description: protein kinase cAMP-dependent type 1 regulatory subunit alpha
Alias: RIIA, cAMP-dependent protein kinase type I-alpha regulatory subunit, protein kinase, cAMP-dependent, regulatory subunit type I alpha, protein kinase, cAMP-dependent, regulatory, type I, alpha
Species: rat
Products:     Prkar1a

Top Publications

  1. Patel H, Hamuro L, Chun B, Kawaraguchi Y, Quick A, Rebolledo B, et al. Disruption of protein kinase A localization using a trans-activator of transcription (TAT)-conjugated A-kinase-anchoring peptide reduces cardiac function. J Biol Chem. 2010;285:27632-40 pubmed publisher
    ..Disruption of PKA localization with TAT-AKAD thus had negative effects on chronotropy, inotropy, and lusitropy, thereby indicating a key role for AKAP-targeted PKA in control of heart rate and contractile function. ..
  2. Burgers P, van der Heyden M, Kok B, Heck A, Scholten A. A systematic evaluation of protein kinase A-A-kinase anchoring protein interaction motifs. Biochemistry. 2015;54:11-21 pubmed publisher
    ..Altogether, THAHIT provides a comprehensive overview of known and novel PKA-AKAP interaction domains and their PKA-R specificities. ..
  3. Kwan E, Gao X, Leung Y, Gaisano H. Activation of exchange protein directly activated by cyclic adenosine monophosphate and protein kinase A regulate common and distinct steps in promoting plasma membrane exocytic and granule-to-granule fusions in rat islet beta cells. Pancreas. 2007;35:e45-54 pubmed
  4. Vigil D, Blumenthal D, Taylor S, Trewhella J. The conformationally dynamic C helix of the RIalpha subunit of protein kinase A mediates isoform-specific domain reorganization upon C subunit binding. J Biol Chem. 2005;280:35521-7 pubmed
  5. Lignitto L, Carlucci A, Sepe M, Stefan E, Cuomo O, Nistico R, et al. Control of PKA stability and signalling by the RING ligase praja2. Nat Cell Biol. 2011;13:412-22 pubmed publisher
    ..Praja2 is required for efficient nuclear cAMP signalling and for PKA-mediated long-term memory. Thus, praja2 regulates the total concentration of R subunits, tuning the strength and duration of PKA signal output in response to cAMP. ..
  6. Greene R, Lloyd M, Uberti M, Nugent P, Pisano M. Patterns of cyclic AMP-dependent protein kinase gene expression during ontogeny of the murine palate. J Cell Physiol. 1995;163:431-40 pubmed
    ..In addition, we have demonstrated adaptational changes of this kinase in MEPM cells in response to conditions of increased intracellular levels of cAMP. ..
  7. Chang W, Rewari A, Centrella M, McCarthy T. Fos-related antigen 2 controls protein kinase A-induced CCAAT/enhancer-binding protein beta expression in osteoblasts. J Biol Chem. 2004;279:42438-44 pubmed
  8. Imaizumi Scherrer T, Faust D, Benichou J, Hellio R, Weiss M. Accumulation in fetal muscle and localization to the neuromuscular junction of cAMP-dependent protein kinase A regulatory and catalytic subunits RI alpha and C alpha. J Cell Biol. 1996;134:1241-54 pubmed
    ..In the adult, the C alpha hybridization signal of muscle is weak and homogeneous...
  9. Kubota H, Katsurabayashi S, Moorhouse A, Murakami N, Koga H, Akaike N. GABAB receptor transduction mechanisms, and cross-talk between protein kinases A and C, in GABAergic terminals synapsing onto neurons of the rat nucleus basalis of Meynert. J Physiol. 2003;551:263-76 pubmed

Scientific Experts

More Information


  1. Yin Z, Jones G, Towns W, Zhang X, Abel E, Binkley P, et al. Heart-specific ablation of Prkar1a causes failure of heart development and myxomagenesis. Circulation. 2008;117:1414-22 pubmed publisher
    ..Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal ..
  2. Kirschner L, Carney J, Pack S, Taymans S, Giatzakis C, Cho Y, et al. Mutations of the gene encoding the protein kinase A type I-alpha regulatory subunit in patients with the Carney complex. Nat Genet. 2000;26:89-92 pubmed
    ..10). In CNC families mapping to 17q, we detected loss of heterozygosity (LOH) in the vicinity of the gene (PRKAR1A) encoding protein kinase A regulatory subunit 1-alpha (RIalpha), including a polymorphic site within its 5' ..
  3. Ruppelt A, Mosenden R, Grönholm M, Aandahl E, Tobin D, Carlson C, et al. Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin. J Immunol. 2007;179:5159-68 pubmed
    ..Finally, small interfering RNA-mediated knockdown of Ezrin abrogates cAMP regulation of IL-2. We propose that Ezrin is essential in the assembly of the cAMP-mediated regulatory pathway that modulates T cell immune responses. ..
  4. Ceccarini M, Grasso M, Veroni C, Gambara G, Artegiani B, Macchia G, et al. Association of dystrobrevin and regulatory subunit of protein kinase A: a new role for dystrobrevin as a scaffold for signaling proteins. J Mol Biol. 2007;371:1174-87 pubmed
    ..Our results suggest a new role for dystrobrevin as a scaffold protein that may play a role in different cellular processes involving PKA signaling. ..
  5. Amieux P, Howe D, Knickerbocker H, Lee D, Su T, Laszlo G, et al. Increased basal cAMP-dependent protein kinase activity inhibits the formation of mesoderm-derived structures in the developing mouse embryo. J Biol Chem. 2002;277:27294-304 pubmed
    ..Our results demonstrate that unregulated PKA activity negatively affects growth factor-mediated mesoderm formation during early mouse development. ..
  6. Lazar S, Galiani D, Dekel N. cAMP-Dependent PKA negatively regulates polyadenylation of c-mos mRNA in rat oocytes. Mol Endocrinol. 2002;16:331-41 pubmed
    ..We also demonstrate that stimulation of Mos synthesis in the rat does not require an active MAPK. ..
  7. Kultgen P, Byrd S, Ostrowski L, Milgram S. Characterization of an A-kinase anchoring protein in human ciliary axonemes. Mol Biol Cell. 2002;13:4156-66 pubmed
    ..These data demonstrate that we have identified the first human axonemal AKAP, a protein that likely plays a role in the signaling necessary for efficient modulation of ciliary beat frequency. ..
  8. Uys G, Ramburan A, Loos B, Kinnear C, Korkie L, Mouton J, et al. Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C. BMC Cell Biol. 2011;12:18 pubmed publisher
  9. Gilbert M, Yang L, Su T, McKnight G. Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype. Am J Physiol Renal Physiol. 2015;308:F627-38 pubmed publisher
    ..These findings demonstrate a distinct physiological role for PKA signaling in both short- and long-term regulation of AQP2 and characterize a novel mouse model of diabetes insipidus. ..
  10. Tasken K, Aandahl E. Localized effects of cAMP mediated by distinct routes of protein kinase A. Physiol Rev. 2004;84:137-67 pubmed
  11. Dwivedi Y, Rizavi H, Pandey G. Differential effects of haloperidol and clozapine on [(3)H]cAMP binding, protein kinase A (PKA) activity, and mRNA and protein expression of selective regulatory and catalytic subunit isoforms of PKA in rat brain. J Pharmacol Exp Ther. 2002;301:197-209 pubmed
  12. Kovo M, Kandli Cohen M, Ben Haim M, Galiani D, Carr D, Dekel N. An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytes. Reproduction. 2006;132:33-43 pubmed
  13. Kovo M, Schillace R, Galiani D, Josefsberg L, Carr D, Dekel N. Expression and modification of PKA and AKAPs during meiosis in rat oocytes. Mol Cell Endocrinol. 2002;192:105-13 pubmed
    ..AKAP140 phosphorylation is sensitive to p34cdc2 kinase inhibitors. We hypothesize that AKAP140 and its phosphorylation state may influence the translocation of the R subunits of PKA throughout resumption of meiosis. ..
  14. Vetter M, Zenn H, Mendez E, van den Boom H, Herberg F, Skålhegg B. The testis-specific C?2 subunit of PKA is kinetically indistinguishable from the common C?1 subunit of PKA. BMC Biochem. 2011;12:40 pubmed publisher
    ..This is also the case for their potency to inhibit catalytic activities of C?2 and C?1. We conclude that the regulatory complexes formed with either C?1 or C?2, respectively, are indistinguishable. ..
  15. Martin N, Whalen E, Zamah M, Pierce K, Lefkowitz R. PKA-mediated phosphorylation of the beta1-adrenergic receptor promotes Gs/Gi switching. Cell Signal. 2004;16:1397-403 pubmed
    ..These results strongly support the hypothesis that the beta(1)-AR, like the beta(2)-AR, can undergo PKA-dependent "G(s)/G(i) switching". ..
  16. Groussin L, Jullian E, Perlemoine K, Louvel A, LeHeup B, Luton J, et al. Mutations of the PRKAR1A gene in Cushing's syndrome due to sporadic primary pigmented nodular adrenocortical disease. J Clin Endocrinol Metab. 2002;87:4324-9 pubmed
    ..Germline mutations of the regulatory subunit R1A of PKA (PRKAR1A) have been observed in about 45% of CNC kindreds...
  17. Mucignat Caretta C, Caretta A. Clustered distribution of cAMP-dependent protein kinase regulatory isoform RI alpha during the development of the rat brain. J Comp Neurol. 2002;451:324-33 pubmed
    ..During the rat's lifespan, the distribution of these peculiar RI alpha clusters undergo changes that may contribute to shape neuronal responses differentially to agents modifying cAMP levels. ..
  18. Gao X, Lin B, Sadayappan S, Patel T. Interactions between the regulatory subunit of type I protein kinase A and p90 ribosomal S6 kinase1 regulate cardiomyocyte apoptosis. Mol Pharmacol. 2014;85:357-67 pubmed publisher
    ..Overall, our findings demonstrate that H/R-mediated decrease in PKARI? protein levels leads to activation of RSK1, which via phosphorylation of NHE1 induces cardiomyocyte apoptosis. ..
  19. Kuno T, Ono Y, Hirai M, Hashimoto S, Shuntoh H, Tanaka C. Molecular cloning and cDNA structure of the regulatory subunit of type I cAMP-dependent protein kinase from rat brain. Biochem Biophys Res Commun. 1987;146:878-83 pubmed
    ..The clone encodes for a protein of 380 amino acids that shows 97% homology to the bovine R-I subunit. Northern blot analysis demonstrated two major mRNA species (2.8 and 4.4 kb in size) in rat brain and liver. ..