Nsf

Summary

Gene Symbol: Nsf
Description: N-ethylmaleimide sensitive factor, vesicle fusing ATPase
Alias: vesicle-fusing ATPase, N-ethylmaleimide-sensitive factor, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, vesicular-fusion protein NSF
Species: rat
Products:     Nsf

Top Publications

  1. Yin S, Guan Z, Tang Y, Zhao J, Hong J, Zhang W. Abnormal expression of epilepsy-related gene ERG1/NSF in the spontaneous recurrent seizure rats with spatial learning memory deficits induced by kainic acid. Brain Res. 2005;1053:195-202 pubmed
    ..SRS)-related gene named epilepsy-related gene (ERG1) that encodes N-ethylmaleimide-sensitive fusion protein (NSF)...
  2. Chen C, Li J, Chen Y, Huang P, Wang Y, Liu Chen L. GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor. J Biol Chem. 2006;281:7983-93 pubmed
    ..GEC1 interacted with N-ethylmaleimide-sensitive factor (NSF) in pull-down assays and co-immunoprecipitated with NSF in rat brain extracts...
  3. Liu Y, Cheng K, Gong K, Fu A, Ip N. Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis. J Biol Chem. 2006;281:9852-8 pubmed
    ..N-Ethylmaleimide-sensitive fusion protein (NSF), a crucial factor in vesicular transport and membrane fusion, was identified as one of the Pctaire1 interacting ..
  4. Xiong H, Cassé F, Zhou M, Xiong Z, Joels M, Martin S, et al. Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus. Hippocampus. 2016;26:848-56 pubmed publisher
    ..Peptides which specifically block the interaction between N-Ethylmaleimide-Sensitive Factor (NSF) and the AMPAR-subunit GluA2 prevented the increase in synaptic transmission and surface expression of AMPARs known ..
  5. Widberg C, Bryant N, Girotti M, Rea S, James D. Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation. J Biol Chem. 2003;278:35093-101 pubmed
    ..These data suggest that Tomosyn and Munc18c operate at a similar stage of the Syntaxin4 SNARE assembly cycle, which likely primes Syntaxin4 for entry into the ternary SNARE complex. ..
  6. Migues P, Hardt O, Finnie P, Wang Y, Nader K. The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2. Hippocampus. 2014;24:1112-9 pubmed publisher
    ..Previous studies have suggested that N-ethylmaleimide-sensitive factor (NSF) regulates the stabilization of AMPARs at the synaptic membrane...
  7. Matveeva E, He P, Whiteheart S. N-Ethylmaleimide-sensitive fusion protein contains high and low affinity ATP-binding sites that are functionally distinct. J Biol Chem. 1997;272:26413-8 pubmed
    N-Ethylmaleimide-sensitive factor (NSF) has been shown to be involved in numerous intracellular membrane fusion events of both the regulated and constitutive secretory pathways...
  8. Bomberger J, Parameswaran N, Hall C, Aiyar N, Spielman W. Novel function for receptor activity-modifying proteins (RAMPs) in post-endocytic receptor trafficking. J Biol Chem. 2005;280:9297-307 pubmed
    ..e. degradation of the receptor complex). Co-expression of N-ethylmaleimide-sensitive factor (NSF) with the CRLR-RAMP3 complex, but not CRLR-RAMP1 or CRLR-RAMP2 complex, altered receptor trafficking to a recycling ..
  9. Nishimune A, Isaac J, Molnar E, Noel J, Nash S, Tagaya M, et al. NSF binding to GluR2 regulates synaptic transmission. Neuron. 1998;21:87-97 pubmed
    Here, we show that N-ethylmaleimide-sensitive fusion protein (NSF) interacts directly and selectively with the intracellular C-terminal domain of the GluR2 subunit of AMPA receptors...

More Information

Publications37

  1. Wyneken U, Smalla K, Marengo J, Soto D, de la Cerda A, Tischmeyer W, et al. Kainate-induced seizures alter protein composition and N-methyl-D-aspartate receptor function of rat forebrain postsynaptic densities. Neuroscience. 2001;102:65-74 pubmed
    ..This rearrangement may accompany impairment of synaptic plasticity. ..
  2. Tani K, Shibata M, Kawase K, Kawashima H, Hatsuzawa K, Nagahama M, et al. Mapping of functional domains of gamma-SNAP. J Biol Chem. 2003;278:13531-8 pubmed
    gamma-Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (gamma-SNAP) is capable of stabilizing a 20 S complex consisting of NSF, alpha-SNAP, and SNAP receptors (SNAREs), but its function in vesicular transport is not ..
  3. Osten P, Srivastava S, Inman G, Vilim F, Khatri L, Lee L, et al. The AMPA receptor GluR2 C terminus can mediate a reversible, ATP-dependent interaction with NSF and alpha- and beta-SNAPs. Neuron. 1998;21:99-110 pubmed
    ..AMPA) receptor subunit C-terminal peptide with an ATPase N-ethylmaleimide-sensitive fusion protein (NSF) and alpha- and beta-soluble NSF attachment proteins (SNAPs), as well as dendritic colocalization of these proteins...
  4. Banerjee A, Li G, Alexander E, Schwartz J. Role of SNAP-23 in trafficking of H+-ATPase in cultured inner medullary collecting duct cells. Am J Physiol Cell Physiol. 2001;280:C775-81 pubmed
    ..We conclude that SNAP-23 is a substrate for botulinum toxin proteolysis and has a critical role in the regulation of H+-ATPase exocytosis and H+ secretion in these renal epithelial cells...
  5. Johnson M, Okunade A. Roles that nurses in the community can play in nursing research. Int Nurs Rev. 1975;22:147-9 pubmed
  6. Huang Y, Man H, Sekine Aizawa Y, Han Y, Juluri K, Luo H, et al. S-nitrosylation of N-ethylmaleimide sensitive factor mediates surface expression of AMPA receptors. Neuron. 2005;46:533-40 pubmed
    ..synaptic plasticity that are modulated by NMDA receptor (NMDAR) activation and N-ethylmaleimide sensitive factor (NSF). We report that NSF is physiologically S-nitrosylated by endogenous, neuronally derived nitric oxide (NO)...
  7. Beretta F, Sala C, Saglietti L, Hirling H, Sheng M, Passafaro M. NSF interaction is important for direct insertion of GluR2 at synaptic sites. Mol Cell Neurosci. 2005;28:650-60 pubmed
    Here, we use a cell surface thrombin cleavage assay to investigate directly the role of NSF in the surface delivery and synaptic accumulation of alpha-amino-3-hydroxy-5-methylisoxazolepropionate (AMPA) receptors...
  8. Hanley J. NSF binds calcium to regulate its interaction with AMPA receptor subunit GluR2. J Neurochem. 2007;101:1644-50 pubmed
    N-ethylmaleimide-sensitive fusion protein (NSF) is essential for numerous Ca(2+)-triggered vesicle trafficking events...
  9. Qu Y, Ma K, Li X. Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk. J Huazhong Univ Sci Technolog Med Sci. 2014;34:801-807 pubmed publisher
    ..The levels of N-ethylmaleimide-sensitive factor (NSF) were measured as well. The results showed that 11 types of proteins were identified by 2D-DIGE...
  10. Zou S, Li L, Pei L, Vukusic B, Van Tol H, Lee F, et al. Protein-protein coupling/uncoupling enables dopamine D2 receptor regulation of AMPA receptor-mediated excitotoxicity. J Neurosci. 2005;25:4385-95 pubmed
    ..between the third intracellular loop of the D2 receptor and the ATPase N-ethylmaleimide-sensitive factor (NSF) while uncoupling the NSF interaction with the carboxyl tail (CT) of the glutamate receptor GluR2 subunit of AMPA ..
  11. Chen X, Matsumoto H, Hinck C, Al Hasani H, St Denis J, Whiteheart S, et al. Demonstration of differential quantitative requirements for NSF among multiple vesicle fusion pathways of GLUT4 using a dominant-negative ATPase-deficient NSF. Biochem Biophys Res Commun. 2005;333:28-34 pubmed
    In this study, we investigated the relative participation of N-ethylmaleimide-sensitive factor (NSF) in vivo in a complex multistep vesicle trafficking system, the translocation response of GLUT4 to insulin in rat adipose cells...
  12. Sangwan V, Abella J, Lai A, Bertos N, Stuible M, Tremblay M, et al. Protein-tyrosine phosphatase 1B modulates early endosome fusion and trafficking of Met and epidermal growth factor receptors. J Biol Chem. 2011;286:45000-13 pubmed publisher
    ..We show that the N-ethylmaleimide-sensitive factor (NSF), an essential component of the vesicle fusion machinery, was hyperphosphorylated in PTP1B knockdown or PTP1B D/A-..
  13. Vivona S, Cipriano D, O Leary S, Li Y, Fenn T, Brunger A. Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between ?-soluble NSF attachment protein (SNAP) and SNARE complex. J Biol Chem. 2013;288:24984-91 pubmed publisher
    Vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase NSF (N-ethylmaleimide-sensitive factor) and the adaptor protein ?-SNAP (soluble NSF attachment protein) disassemble all SNARE complexes ..
  14. Périer F, Coulter K, Liang H, Radeke C, Gaber R, Vandenberg C. Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast. FEBS Lett. 1994;351:286-90 pubmed
    ..encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Pas1p), and transcriptional regulation (TBP-1)...
  15. Martin H, Henley J, Meyer G. Novel putative targets of N-ethylmaleimide sensitive fusion protein (NSF) and alpha/beta soluble NSF attachment proteins (SNAPs) include the Pak-binding nucleotide exchange factor betaPIX. J Cell Biochem. 2006;99:1203-15 pubmed
    N-ethylmaleimide sensitive fusion protein (NSF) is a chaperone that plays a crucial role in the fusion of vesicles with target membranes...
  16. Narisawa Saito M, Iwakura Y, Kawamura M, Araki K, Kozaki S, Takei N, et al. Brain-derived neurotrophic factor regulates surface expression of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors by enhancing the N-ethylmaleimide-sensitive factor/GluR2 interaction in developing neocortical neurons. J Biol Chem. 2002;277:40901-10 pubmed
    ..These results reveal a novel role of BDNF in regulating the surface expression of AMPA receptors through a GluR2-NSF interaction.
  17. Hanley J, Khatri L, Hanson P, Ziff E. NSF ATPase and alpha-/beta-SNAPs disassemble the AMPA receptor-PICK1 complex. Neuron. 2002;34:53-67 pubmed
    AMPA receptor (AMPAR) trafficking is crucial for synaptic plasticity that may be important for learning and memory. NSF and PICK1 bind the AMPAR GluR2 subunit and are involved in trafficking of AMPARs...
  18. Cong M, Perry S, Hu L, Hanson P, Claing A, Lefkowitz R. Binding of the beta2 adrenergic receptor to N-ethylmaleimide-sensitive factor regulates receptor recycling. J Biol Chem. 2001;276:45145-52 pubmed
    ..acids in its carboxyl-terminal tail to the membrane fusion regulatory protein, N-ethylmaleimide-sensitive factor (NSF). This is documented by in vitro overlay assays and by cellular coimmunoprecipitations...
  19. McDonald P, Cote N, Lin F, Premont R, Pitcher J, Lefkowitz R. Identification of NSF as a beta-arrestin1-binding protein. Implications for beta2-adrenergic receptor regulation. J Biol Chem. 1999;274:10677-80 pubmed
    ..Using the yeast two-hybrid system, we have identified a novel beta-arrestin1-binding protein, NSF (N-ethylmaleimide-sensitive fusion protein), an ATPase essential for many intracellular transport reactions...
  20. Katano T, Furue H, Okuda Ashitaka E, Tagaya M, Watanabe M, Yoshimura M, et al. N-ethylmaleimide-sensitive fusion protein (NSF) is involved in central sensitization in the spinal cord through GluR2 subunit composition switch after inflammation. Eur J Neurosci. 2008;27:3161-70 pubmed publisher
    ..N-ethylmaleimide-sensitive fusion protein (NSF) was identified as a downregulated protein in the PSD by MALDI-TOF MS and immunoblotting...
  21. Chen S, Liu F. Interaction of dopamine D1 receptor with N-ethylmaleimide-sensitive factor is important for the membrane localization of the receptor. J Neurosci Res. 2010;88:2504-12 pubmed publisher
    ..Here, we characterize the interaction between D1R and N-ethylmaleimide-sensitive factor (NSF) and show that the interaction is mediated by aa 387-401 of the D1R C-terminal tail...
  22. Joels G, Lamprecht R. Interaction between N-ethylmaleimide-sensitive factor and GluR2 is essential for fear memory formation in lateral amygdala. J Neurosci. 2010;30:15981-6 pubmed publisher
    ..Maintaining GluR2/GluR3 receptors level in synapse requires interactions of N-ethylmaleimide-sensitive factor (NSF) with GluR2...
  23. Han S, Park D, Park S, Hong S. Identification of Rab6 as an N-ethylmaleimide-sensitive fusion protein-binding protein. Biochem J. 2000;352 Pt 1:165-73 pubmed
    In this study we show the interaction of N-ethylmaleimide-sensitive fusion protein (NSF) with a small GTP-binding protein, Rab6. NSF is an ATPase involved in the vesicular transport within eukaryotic cells...
  24. Hong H, Chakravarti A, Takahashi J. The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice. Proc Natl Acad Sci U S A. 2004;101:1748-53 pubmed
    ..The hyh mutant provides a valuable in vivo model to study vesicle/membrane trafficking and provides insight into the potential roles of alpha-SNAP in embryogenesis and brain development. ..
  25. Lu H, Wu P, Yang Y, Xiao W, Fan J, Liu J, et al. Interactions between N-ethylmaleimide-sensitive factor and GluR2 in the nucleus accumbens contribute to the expression of locomotor sensitization to cocaine. J Neurosci. 2014;34:3493-508 pubmed publisher
    ..that the expression of behavioral sensitization was negatively controlled by N-ethylmaleimide-sensitive factor (NSF)-GluR2 interactions in the NAc...
  26. Hanson P, Roth R, Morisaki H, Jahn R, Heuser J. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy. Cell. 1997;90:523-35 pubmed
    Using quick-freeze/deep-etch electron microscopy of recombinant proteins adsorbed to mica, we show that NSF, the oligomeric ATPase involved in membrane fusion, is a hollow 10 x 16 nm cylinder whose conformation depends upon nucleotide ..
  27. Pontier S, Lahaie N, Ginham R, St Gelais F, Bonin H, Bell D, et al. Coordinated action of NSF and PKC regulates GABAB receptor signaling efficacy. EMBO J. 2006;25:2698-709 pubmed
    ..Here, we show that NEM sensitive fusion (NSF) protein interacts directly with the GBR heterodimer both in rat brain synaptosomes and in CHO cells, forming a ..
  28. Gómez Chacón B, Gámiz F, Foster T, Gallo M. Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia. Neural Plast. 2016;2016:2726745 pubmed publisher
    Interactions between GluR2 and N-ethylmaleimide-sensitive factor (NSF) mediate AMPA receptors trafficking. This might be linked with molecular mechanisms related with memory formation...