Nos3

Summary

Gene Symbol: Nos3
Description: nitric oxide synthase 3
Alias: eNos, nitric oxide synthase, endothelial, EC-NOS, NOS type III, NOSIII, cNOS, constitutive NOS, endothelial NOS, endothelial nitric oxide synthase 3, nitric oxide synthase 3, endothelial cell
Species: rat
Products:     Nos3

Top Publications

  1. Jiang L, Quarck R, Janssens S, Pokreisz P, Demedts M, Delcroix M. Effect of adenovirus-mediated gene transfer of nitric oxide synthase on vascular reactivity of rat isolated pulmonary arteries. Pflugers Arch. 2006;452:213-21 pubmed
    Aerosol gene transfer of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) to rat lungs increased NOS expression and activity, and prevented hypoxic pulmonary vasoconstriction (HPV) in vivo...
  2. de Syllos R, Sandrim V, Lisboa H, Tres G, Tanus Santos J. Endothelial nitric oxide synthase genotype and haplotype are not associated with diabetic retinopathy in diabetes type 2 patients. Nitric Oxide. 2006;15:417-22 pubmed
    Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been associated with the development of diabetic retinopathy (DR) in patients with type 1 diabetes mellitus (T1DM), but not with T2DM...
  3. Li Q, Verma A, Han P, Nakagawa T, Johnson R, Grant M, et al. Diabetic eNOS-knockout mice develop accelerated retinopathy. Invest Ophthalmol Vis Sci. 2010;51:5240-6 pubmed publisher
    Dysfunction of endothelial nitric oxide synthase (eNOS) has been implicated in the pathogenesis of diabetic vascular complications...
  4. Kobayashi T, Taguchi K, Nemoto S, Nogami T, Matsumoto T, Kamata K. Activation of the PDK-1/Akt/eNOS pathway involved in aortic endothelial function differs between hyperinsulinemic and insulin-deficient diabetic rats. Am J Physiol Heart Circ Physiol. 2009;297:H1767-75 pubmed publisher
    ..In the diabetic group, various insulin-stimulated levels (nitric oxide production, phosphorylation of endothelial NOS at Ser(1177), of Akt at Thr(308), and of PDK-1 at Ser(241)) were significantly increased, whereas, in the HI-..
  5. Ayub H, Khan M, Micheal S, Akhtar F, Ajmal M, Shafique S, et al. Association of eNOS and HSP70 gene polymorphisms with glaucoma in Pakistani cohorts. Mol Vis. 2010;16:18-25 pubmed
    To investigate the involvement of stress-regulating genes, endothelial nitric oxide synthase (eNOS) and heat shock protein 70 (HSP70) with primary open angle glaucoma (POAG) and primary closed angle glaucoma (PCAG)...
  6. Herrera M, Hong N, Ortiz P, Garvin J. Endothelin-1 inhibits thick ascending limb transport via Akt-stimulated nitric oxide production. J Biol Chem. 2009;284:1454-60 pubmed publisher
    ..NO production by activating phosphatidylinositol 3-kinase (PI3K), stimulating Akt activity, and phosphorylating NOS3 at Ser1177. This enhances NO production and inhibits sodium transport...
  7. Yanamandra K, Boggs P, Thurmon T, Lewis D, Bocchini J, Dhanireddy R. Novel allele of the endothelial nitric oxide synthase gene polymorphism in Caucasian asthmatics. Biochem Biophys Res Commun. 2005;335:545-9 pubmed
    The purpose of the present study was to investigate an association of the endothelial nitric oxide synthase (eNOS) gene polymorphism with asthma...
  8. Joshi M, Ferguson T, Johnson F, Johnson R, Parthasarathy S, Lancaster J. Receptor-mediated activation of nitric oxide synthesis by arginine in endothelial cells. Proc Natl Acad Sci U S A. 2007;104:9982-7 pubmed
    ..evidence for binding of arginine to membrane receptor(s), leading to the activation of endothelial NO synthase (eNOS) NO production through a second messenger pathway...
  9. Dumont O, Loufrani L, Henrion D. Key role of the NO-pathway and matrix metalloprotease-9 in high blood flow-induced remodeling of rat resistance arteries. Arterioscler Thromb Vasc Biol. 2007;27:317-24 pubmed
    ..After 14 days, outward hypertrophic remodeling occurred in HF arteries in association with eNOS overexpression. MMP9 activity increased in the early phase (day 4)...
  10. Dimmeler S, Fleming I, Fisslthaler B, Hermann C, Busse R, Zeiher A. Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature. 1999;399:601-5 pubmed
    Nitric oxide (NO) produced by the endothelial NO synthase (eNOS) is a fundamental determinant of cardiovascular homesotasis: it regulates systemic blood pressure, vascular remodelling and angiogenesis...

Detail Information

Publications62

  1. Jiang L, Quarck R, Janssens S, Pokreisz P, Demedts M, Delcroix M. Effect of adenovirus-mediated gene transfer of nitric oxide synthase on vascular reactivity of rat isolated pulmonary arteries. Pflugers Arch. 2006;452:213-21 pubmed
    Aerosol gene transfer of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) to rat lungs increased NOS expression and activity, and prevented hypoxic pulmonary vasoconstriction (HPV) in vivo...
  2. de Syllos R, Sandrim V, Lisboa H, Tres G, Tanus Santos J. Endothelial nitric oxide synthase genotype and haplotype are not associated with diabetic retinopathy in diabetes type 2 patients. Nitric Oxide. 2006;15:417-22 pubmed
    Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been associated with the development of diabetic retinopathy (DR) in patients with type 1 diabetes mellitus (T1DM), but not with T2DM...
  3. Li Q, Verma A, Han P, Nakagawa T, Johnson R, Grant M, et al. Diabetic eNOS-knockout mice develop accelerated retinopathy. Invest Ophthalmol Vis Sci. 2010;51:5240-6 pubmed publisher
    Dysfunction of endothelial nitric oxide synthase (eNOS) has been implicated in the pathogenesis of diabetic vascular complications...
  4. Kobayashi T, Taguchi K, Nemoto S, Nogami T, Matsumoto T, Kamata K. Activation of the PDK-1/Akt/eNOS pathway involved in aortic endothelial function differs between hyperinsulinemic and insulin-deficient diabetic rats. Am J Physiol Heart Circ Physiol. 2009;297:H1767-75 pubmed publisher
    ..In the diabetic group, various insulin-stimulated levels (nitric oxide production, phosphorylation of endothelial NOS at Ser(1177), of Akt at Thr(308), and of PDK-1 at Ser(241)) were significantly increased, whereas, in the HI-..
  5. Ayub H, Khan M, Micheal S, Akhtar F, Ajmal M, Shafique S, et al. Association of eNOS and HSP70 gene polymorphisms with glaucoma in Pakistani cohorts. Mol Vis. 2010;16:18-25 pubmed
    To investigate the involvement of stress-regulating genes, endothelial nitric oxide synthase (eNOS) and heat shock protein 70 (HSP70) with primary open angle glaucoma (POAG) and primary closed angle glaucoma (PCAG)...
  6. Herrera M, Hong N, Ortiz P, Garvin J. Endothelin-1 inhibits thick ascending limb transport via Akt-stimulated nitric oxide production. J Biol Chem. 2009;284:1454-60 pubmed publisher
    ..NO production by activating phosphatidylinositol 3-kinase (PI3K), stimulating Akt activity, and phosphorylating NOS3 at Ser1177. This enhances NO production and inhibits sodium transport...
  7. Yanamandra K, Boggs P, Thurmon T, Lewis D, Bocchini J, Dhanireddy R. Novel allele of the endothelial nitric oxide synthase gene polymorphism in Caucasian asthmatics. Biochem Biophys Res Commun. 2005;335:545-9 pubmed
    The purpose of the present study was to investigate an association of the endothelial nitric oxide synthase (eNOS) gene polymorphism with asthma...
  8. Joshi M, Ferguson T, Johnson F, Johnson R, Parthasarathy S, Lancaster J. Receptor-mediated activation of nitric oxide synthesis by arginine in endothelial cells. Proc Natl Acad Sci U S A. 2007;104:9982-7 pubmed
    ..evidence for binding of arginine to membrane receptor(s), leading to the activation of endothelial NO synthase (eNOS) NO production through a second messenger pathway...
  9. Dumont O, Loufrani L, Henrion D. Key role of the NO-pathway and matrix metalloprotease-9 in high blood flow-induced remodeling of rat resistance arteries. Arterioscler Thromb Vasc Biol. 2007;27:317-24 pubmed
    ..After 14 days, outward hypertrophic remodeling occurred in HF arteries in association with eNOS overexpression. MMP9 activity increased in the early phase (day 4)...
  10. Dimmeler S, Fleming I, Fisslthaler B, Hermann C, Busse R, Zeiher A. Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature. 1999;399:601-5 pubmed
    Nitric oxide (NO) produced by the endothelial NO synthase (eNOS) is a fundamental determinant of cardiovascular homesotasis: it regulates systemic blood pressure, vascular remodelling and angiogenesis...
  11. Kobayashi K, Nishimura Y, Yamashita T, Nishiuma T, Satouchi M, Yokoyama M. The effect of overexpression of endothelial nitric oxide synthase on eosinophilic lung inflammation in a murine model. Int Immunopharmacol. 2006;6:1040-52 pubmed
    ..In particular, the role of endothelial nitric oxide synthase (eNOS) in asthma remains uncertain...
  12. Francis B, Salameh M, Khamisy Farah R, Farah R. Tetrahydrobiopterin (BH4 ): Targeting endothelial Nitric Oxide Synthase as a potential therapy for Pulmonary Hypertension. Cardiovasc Ther. 2017;: pubmed publisher
    ..Tetrahydrobiopterin (BH4 ) regulates endothelial nitric oxide synthase (eNOS) to produce nitric oxide rather than superoxide which maintains normal endothelial and cardiac function...
  13. An J, Du J, Wei N, Xu H, Pritchard K, Shi Y. Role of tetrahydrobiopterin in resistance to myocardial ischemia in Brown Norway and Dahl S rats. Am J Physiol Heart Circ Physiol. 2009;297:H1783-91 pubmed publisher
    ..generation secondary to increased heat shock protein 90 (HSP90) association with endothelial nitric oxide synthase (NOS3)...
  14. Ramachandran R, Ploug K, Hay Schmidt A, Olesen J, Jansen Olesen I, Gupta S. Nitric oxide synthase (NOS) in the trigeminal vascular system and other brain structures related to pain in rats. Neurosci Lett. 2010;484:192-6 pubmed publisher
    ..quantitative real-time PCR and Western blotting to measure the respective levels of mRNA and protein for nNOS and eNOS in peripheral and central tissues involved in migraine pain: dura mater, pial arteries, trigeminal ganglion (TG) ..
  15. Romero M, Jimenez R, Sanchez M, López Sepúlveda R, Zarzuelo M, O Valle F, et al. Quercetin inhibits vascular superoxide production induced by endothelin-1: Role of NADPH oxidase, uncoupled eNOS and PKC. Atherosclerosis. 2009;202:58-67 pubmed publisher
    ..Taken together these results indicate that ET-1-induced NADPH oxidase up-regulation and eNOS uncoupling via PKC leading to endothelial dysfunction and these effects were prevented by quercetin and ..
  16. Kumar S, Jyoti A, Keshari R, Singh M, Barthwal M, Dikshit M. Functional and molecular characterization of NOS isoforms in rat neutrophil precursor cells. Cytometry A. 2010;77:467-77 pubmed publisher
    ..iNOS expression was, however, consistently augmented during maturation, whereas eNOS expression was downregulated with neutrophil maturation...
  17. Ošiņa K, Leonova E, Isajevs S, Baumane L, Rostoka E, Sjakste T, et al. Modifications of expression of genes and proteins involved in DNA repair and nitric oxide metabolism by carbatonides [disodium-2,6-dimethyl-1,4-dihydropyridine- 3,5-bis(carbonyloxyacetate) derivatives] in intact and diabetic rats. Arh Hig Rada Toksikol. 2017;68:212-227 pubmed publisher
    ..Metcarbatone and etcarbatone up-regulated mRNA and protein of eNOS in kidneys of diabetic animals; etcarbatone also in myocardium...
  18. Ren Y, Sun L, Shao X, Chen J, Xiong B, Nong L. [Expressions of eNOS and cytochrome P450 in the testis of sexually mature SD rats and their significance]. Zhonghua Nan Ke Xue. 2009;15:911-4 pubmed
    To explore the expressions of endothelial nitric oxide synthase (eNOS) and cytochrome P450 (aromatase) in the testis of sexually mature male SD rats and their significance...
  19. Kim J, Bugaj L, Oh Y, Bivalacqua T, Ryoo S, Soucy K, et al. Arginase inhibition restores NOS coupling and reverses endothelial dysfunction and vascular stiffness in old rats. J Appl Physiol (1985). 2009;107:1249-57 pubmed publisher
    ..In this study, we demonstrate that arginase upregulation leads to endothelial nitric oxide synthase (eNOS) uncoupling and that in vivo chronic inhibition of arginase restores nitroso-redox balance, improves endothelial ..
  20. Suzuki T, Mori C, Yoshikawa H, Miyazaki Y, Kansaku N, Tanaka K, et al. Changes in nitric oxide production levels and expression of nitric oxide synthase isoforms in the rat uterus during pregnancy. Biosci Biotechnol Biochem. 2009;73:2163-6 pubmed
    ..Thus NOS-2-generated NO in the decidua contributed significantly to uterine NO levels...
  21. Waki H, Murphy D, Yao S, Kasparov S, Paton J. Endothelial NO synthase activity in nucleus tractus solitarii contributes to hypertension in spontaneously hypertensive rats. Hypertension. 2006;48:644-50 pubmed
    ..Based on previous data, we hypothesized that overactivity of endothelial NO synthase (eNOS) within the nucleus tractus solitarii (NTS) could contribute to the hypertension in the spontaneously hypertensive ..
  22. Yar A, Menevse S, Dogan I, Alp E, Ergin V, Cumaoglu A, et al. Investigation of ocular neovascularization-related genes and oxidative stress in diabetic rat eye tissues after resveratrol treatment. J Med Food. 2012;15:391-8 pubmed publisher
    ..angiotensin-converting enzyme (ACE), matrix metalloproteinase (MMP)-9, and endothelial nitric oxide synthase (eNOS) mRNA expression profiles and oxidative stress in the eye tissue microenviroment may have important roles in ocular ..
  23. Noguchi K, Hamadate N, Matsuzaki T, Sakanashi M, Nakasone J, Uchida T, et al. Increasing dihydrobiopterin causes dysfunction of endothelial nitric oxide synthase in rats in vivo. Am J Physiol Heart Circ Physiol. 2011;301:H721-9 pubmed publisher
    ..of oxidative stress, such as arteriosclerotic/atherosclerotic disorders, where endothelial nitric oxide synthase (eNOS) is dysfunctional, but the role of BH(2) in the regulation of eNOS activity in vivo remains to be evaluated...
  24. Huang S, Lu Y, Huang J, Wu Y, Day Y, Hung L. The essential role of endothelial nitric oxide synthase activation in insulin-mediated neuroprotection against ischemic stroke in diabetes. J Vasc Surg. 2014;59:483-91 pubmed publisher
    ..membrane-trafficking activity of glucose transporter 1, and reduction of Akt and endothelial nitric oxide synthase (eNOS) phosphorylation in the cerebrum...
  25. Chan Y, Leung F, Wong W, Tian X, Yung L, Lau C, et al. Therapeutically relevant concentrations of raloxifene dilate pressurized rat resistance arteries via calcium-dependent endothelial nitric oxide synthase activation. Arterioscler Thromb Vasc Biol. 2010;30:992-9 pubmed publisher
    ..The present study aimed to examine roles of Ca(2+) influx in endothelium and endothelial nitric oxide synthase (eNOS) activation in dilatations induced by raloxifene, a second-generation SERM in myogenically active arteries...
  26. Patel N, Nandra K, Brines M, Collino M, Wong W, Kapoor A, et al. A nonerythropoietic peptide that mimics the 3D structure of erythropoietin reduces organ injury/dysfunction and inflammation in experimental hemorrhagic shock. Mol Med. 2011;17:883-92 pubmed publisher
    ....
  27. Thum T, Fraccarollo D, Schultheiss M, Froese S, Galuppo P, Widder J, et al. Endothelial nitric oxide synthase uncoupling impairs endothelial progenitor cell mobilization and function in diabetes. Diabetes. 2007;56:666-74 pubmed
    Uncoupling of the endothelial nitric oxide synthase (eNOS) resulting in superoxide anion (O(2)(-)) formation instead of nitric oxide (NO) causes diabetic endothelial dysfunction...
  28. Gao L, Yu D. Molecular mechanism of limbs' postischemic revascularization improved by perindopril in diabetic rats. Chin Med J (Engl). 2008;121:2129-33 pubmed
    ..eNOS), vascular endothelial growth factor (VEGF) and basic fibroblast (bFGF), constitutive nitric oxide synthase (cNOS) activity and nitric oxide (NO) content were observed after treatment...
  29. Sasaki A, Doi S, Mizutani S, Azuma H. Roles of accumulated endogenous nitric oxide synthase inhibitors, enhanced arginase activity, and attenuated nitric oxide synthase activity in endothelial cells for pulmonary hypertension in rats. Am J Physiol Lung Cell Mol Physiol. 2007;292:L1480-7 pubmed
    ..The constitutive NOS activity and protein expression in PAECs were significantly reduced in MCT group, whereas the arginase, which ..
  30. Kim Y, Hwang J, Noh J, Gang G, Kim D, Son H, et al. Activation of NAD(P)H:quinone oxidoreductase ameliorates spontaneous hypertension in an animal model via modulation of eNOS activity. Cardiovasc Res. 2011;91:519-27 pubmed publisher
    ..Nitric oxide (NO) produced by endothelial NO synthase (eNOS) is an important regulator of blood pressure (BP)...
  31. Jankowski M, Wang D, Mukaddam Daher S, Gutkowska J. Pregnancy alters nitric oxide synthase and natriuretic peptide systems in the rat left ventricle. J Endocrinol. 2005;184:209-17 pubmed
    ..01 +/- 0.15 pmol/g) and day 4 (9.21 +/- 1.79 pmol/g). Changes in endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), OTR and ERalpha, but not ERbeta, proteins paralleled the pregnancy-related cGMP changes in ..
  32. Song W, Kwak H, Kim J, Lawler J. Exercise training modulates the nitric oxide synthase profile in skeletal muscle from old rats. J Gerontol A Biol Sci Med Sci. 2009;64:540-9 pubmed publisher
    ..b>Endothelial NOS (eNOS) protein levels were depressed in WG of old rats but were higher in OE than in OS...
  33. Zhao J, Wecht J, Zhang Y, Wen X, Zeman R, Bauman W, et al. iNOS expression in rat aorta is increased after spinal cord transection: a possible cause of orthostatic hypotension in man. Neurosci Lett. 2007;415:210-4 pubmed
    ..Expression in thoracic aortic of inducible (iNOS), endothelial (eNOS) and neuronal (nNOS) NOS was then determined...
  34. Bonnin P, Leger P, Villapol S, Deroide N, Gressens P, Pocard M, et al. Dual action of NO synthases on blood flow and infarct volume consecutive to neonatal focal cerebral ischemia. Exp Neurol. 2012;236:50-7 pubmed publisher
    ..Conversely, endothelial NOS inhibition increased blood-flows and aggravated volume lesion in males, whereas in females blood-flows did ..
  35. Han F, Shirasaki Y, Fukunaga K. Microsphere embolism-induced endothelial nitric oxide synthase expression mediates disruption of the blood-brain barrier in rat brain. J Neurochem. 2006;99:97-106 pubmed
    Microsphere embolism (ME)-induced up-regulation of endothelial nitric oxide synthase (eNOS) in endothelial cells of brain microvessels was observed 2-48 h after ischemia...