Gene Symbol: Ncf1
Description: neutrophil cytosolic factor 1
Alias: Ncf-1, p47phox, neutrophil cytosolic factor 1, neutrophil cytosol factor 1, phagocyte oxidase 47 kDa
Species: rat
Products:     Ncf1

Top Publications

  1. Olofsson P, Johansson A, Wedekind D, Kloting I, Klinga Levan K, Lu S, et al. Inconsistent susceptibility to autoimmunity in inbred LEW rats is due to genetic crossbreeding involving segregation of the arthritis-regulating gene Ncf1. Genomics. 2004;83:765-71 pubmed
    We recently identified a single-nucleotide polymorphism in the Ncf1 gene, a component of the NADPH oxidase complex, to be the cause of one of the strongest identified loci for arthritis severity in rats...
  2. Hultqvist M, Holmdahl R. Ncf1 (p47phox) polymorphism determines oxidative burst and the severity of arthritis in rats and mice. Cell Immunol. 2005;233:97-101 pubmed
    ..By using an alternative approach, i.e., linkage analysis using relevant animal models we succeeded in finding the Ncf1 gene residing in the Pia4 quantitative trait locus to be responsible for the severity of pristane induced arthritis ..
  3. Olofsson P, Holmberg J, Tordsson J, Lu S, Akerstrom B, Holmdahl R. Positional identification of Ncf1 as a gene that regulates arthritis severity in rats. Nat Genet. 2003;33:25-32 pubmed
    ..cloning of the Pia4 QTL in rats, we found that a naturally occurring polymorphism of Ncf1 (encoding neutrophil cytosolic factor 1, a component of the NADPH oxidase complex) regulates arthritis severity...
  4. Syed I, Kyathanahalli C, Kowluru A. Phagocyte-like NADPH oxidase generates ROS in INS 832/13 cells and rat islets: role of protein prenylation. Am J Physiol Regul Integr Comp Physiol. 2011;300:R756-62 pubmed publisher
    ..Together, our findings suggest a prenylated Ptx-sensitive signaling step couples Rac1 activation in the signaling steps necessary for glucose-mediated generation of ROS in the pancreatic ?-cells. ..
  5. Kim M, Shin K, Chung Y, Jung K, Cha C, Shin D. Immunohistochemical study of p47Phox and gp91Phox distributions in rat brain. Brain Res. 2005;1040:178-86 pubmed
    NADPH oxidase is multi-component enzyme, which comprises the cytosolic proteins p40Phox, p47Phox, and p67Phox and the two membrane proteins, gp91Phox and p22Phox, and which is well characterized in phagocytic cells...
  6. Hultqvist M, Sareila O, Vilhardt F, Norin U, Olsson L, Olofsson P, et al. Positioning of a polymorphic quantitative trait nucleotide in the Ncf1 gene controlling oxidative burst response and arthritis severity in rats. Antioxid Redox Signal. 2011;14:2373-83 pubmed publisher
    The Ncf1 gene, encoding the P47(PHOX) protein that regulates production of reactive oxygen species (ROS) by the phagocyte NADPH oxidase (NOX2) complex, is associated with autoimmunity and arthritis severity in rats...
  7. Pithon Curi T, Levada A, Lopes L, Doi S, Curi R. Glutamine plays a role in superoxide production and the expression of p47phox, p22phox and gp91phox in rat neutrophils. Clin Sci (Lond). 2002;103:403-8 pubmed
    ..We postulate that glutamine leads to O(2)(-) production in neutrophils, probably via the generation of ATP and regulation of the expression of components of NADPH oxidase. ..
  8. Francke U, Hsieh C, Foellmer B, Lomax K, Malech H, Leto T. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1). Am J Hum Genet. 1990;47:483-92 pubmed
    ..recessive forms of CGD result from defects in cytosolic components of the phagocyte NADPH oxidase system, p47phox (NCF1) and p67phox (NCF2)...
  9. Glass M, Chan J, Frys K, Oselkin M, Tarsitano M, Iadecola C, et al. Changes in the subcellular distribution of NADPH oxidase subunit p47phox in dendrites of rat dorsomedial nucleus tractus solitarius neurons in response to chronic administration of hypertensive agents. Exp Neurol. 2007;205:383-95 pubmed

More Information


  1. Glass M, Huang J, Oselkin M, Tarsitano M, Wang G, Iadecola C, et al. Subcellular localization of nicotinamide adenine dinucleotide phosphate oxidase subunits in neurons and astroglia of the rat medial nucleus tractus solitarius: relationship with tyrosine hydroxylase immunoreactive neurons. Neuroscience. 2006;143:547-64 pubmed
    ..Moreover, NADPH oxidase-derived superoxide in neurons and glia may directly or indirectly modulate catecholaminergic neuron activity in the mNTS. ..
  2. Chignalia A, Schuldt E, Camargo L, Montezano A, Callera G, Laurindo F, et al. Testosterone induces vascular smooth muscle cell migration by NADPH oxidase and c-Src-dependent pathways. Hypertension. 2012;59:1263-71 pubmed publisher
    ..Testosterone increased Nox1 and Nox4 mRNA levels and p47phox protein expression, determined by real-time PCR and immunoblotting, respectively...
  3. Serizawa K, Yogo K, Aizawa K, Tashiro Y, Ishizuka N. Nicorandil prevents endothelial dysfunction due to antioxidative effects via normalisation of NADPH oxidase and nitric oxide synthase in streptozotocin diabetic rats. Cardiovasc Diabetol. 2011;10:105 pubmed publisher
    ..7 ± 1.4%; normal, 19.5 ± 1.7%; n = 6-7). There was a 2.4-fold increase in p47phox expression, a subunit of NADPH oxidase, and a 1...
  4. Espinosa A, Leiva A, Peña M, Müller M, Debandi A, Hidalgo C, et al. Myotube depolarization generates reactive oxygen species through NAD(P)H oxidase; ROS-elicited Ca2+ stimulates ERK, CREB, early genes. J Cell Physiol. 2006;209:379-88 pubmed
    ..Myotubes contained both the p47phox and gp91phox phagocytic NAD(P)H oxidase subunits, as revealed by immunodetection...
  5. Karathanassis D, Stahelin R, Bravo J, Perisic O, Pacold C, Cho W, et al. Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction. EMBO J. 2002;21:5057-68 pubmed
  6. He L, Liu X, Chen J, Dinger B, Stensaas L, Fidone S. Modulation of chronic hypoxia-induced chemoreceptor hypersensitivity by NADPH oxidase subunits in rat carotid body. J Appl Physiol (1985). 2010;108:1304-10 pubmed publisher
    ..5-fold) and NOX4 (approximately 3.8-fold) and also increased presence of the regulatory subunit p47phox (approximately 4.2-fold)...
  7. Reinehr R, Sommerfeld A, Keitel V, Grether Beck S, Haussinger D. Amplification of CD95 activation by caspase 8-induced endosomal acidification in rat hepatocytes. J Biol Chem. 2008;283:2211-22 pubmed
    ..This autoamplification of CD95-activation is required for apoptosis induction. ..
  8. Ding S, Yang J, Liu L, Ye Y, Wang X, Hu J, et al. Elevated dopamine induces minimal hepatic encephalopathy by activation of astrocytic NADPH oxidase and astrocytic protein tyrosine nitration. Int J Biochem Cell Biol. 2014;55:252-63 pubmed publisher
    ..PTN induced by DA, which was attributed to NADPH oxidase and not to nNOS, may alter astrocyte function and thereby contribute to the precipitation of MHE episodes. ..
  9. Rusyn I, Yamashina S, Segal B, Schoonhoven R, Holland S, Cattley R, et al. Oxidants from nicotinamide adenine dinucleotide phosphate oxidase are involved in triggering cell proliferation in the liver due to peroxisome proliferators. Cancer Res. 2000;60:4798-803 pubmed
    ..b>p47phox is a critical subunit of NADPH OX; therefore, p47phox knockout mice were used to specifically address the ..
  10. Sipkens J, Krijnen P, Hahn N, Wassink M, Meischl C, Smith D, et al. Homocysteine-induced cardiomyocyte apoptosis and plasma membrane flip-flop are independent of S-adenosylhomocysteine: a crucial role for nuclear p47(phox). Mol Cell Biochem. 2011;358:229-39 pubmed publisher
    ..Incubation with SAH did not have an effect on cell viability, nor on flippase activity, nor on nuclear NOX2-, p47phox expression or nuclear ROS production...
  11. Tanabe M, Radmark O, Watanabe T, Shiose A, Sumimoto H. Cloning of rat p47phox and comparison with human p47phox. DNA Seq. 2005;16:65-8 pubmed
    A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends...
  12. Jackson S, Devadas S, Kwon J, Pinto L, Williams M. T cells express a phagocyte-type NADPH oxidase that is activated after T cell receptor stimulation. Nat Immunol. 2004;5:818-27 pubmed
    ..Thus, mature T cells express a phagocyte-type NADPH oxidase that regulates elements of TCR signaling. ..
  13. Huberle A, Beyeen A, Ockinger J, Ayturan M, Jagodic M, de Graaf K, et al. Advanced intercross line mapping suggests that ncf1 (ean6) regulates severity in an animal model of guillain-barre syndrome. J Immunol. 2009;182:4432-8 pubmed publisher
    ..3 Mb on RNO12, harboring the neutrophil cytosolic factor 1 (Ncf1) among other genes...
  14. Lomax K, Leto T, Nunoi H, Gallin J, Malech H. Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease. Science. 1989;245:409-12 pubmed
    ..Recombinant NCF-47k restored O2-. -producing activity to AR-CGD neutrophil cytosol in a cell-free assay. Production of active recombinant NCF-47k will enable functional regions of this molecule to be mapped. ..
  15. Maeda K, Yasunari K, Sato E, Yoshikawa J, Inoue M. Activation of protein kinase C and nicotinamide adenine dinucleotide phosphate oxidase in leukocytes of spontaneously hypertensive rats. Hypertens Res. 2003;26:999-1006 pubmed
    ..Furthermore, expression of p47phox of nicotinamide adenine dinucleotide phosphate oxidase, but not of p67phox, in PMN from SHR was higher than that ..
  16. Xie X, Liu H, Fan J, Li H. [Effects of ginsenoside Rb1 on TGF-beta1 induced p47phox expression and extracellular matrix accumulation in rat renal tubular epethelial cells]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2009;40:106-10 pubmed
    ..Intracellular reactive oxidative species (ROS) level was measured by flowcytometry. p47phox protein expression was assessed by immunohistochemistry and western blotting method...
  17. Shukla S, Jha R, Laloraya M, Kumar P. Identification of non-mitochondrial NADPH oxidase and the spatio-temporal organization of its components in mouse spermatozoa. Biochem Biophys Res Commun. 2005;331:476-83 pubmed
  18. Tkalcevic J, Novelli M, Phylactides M, Iredale J, Segal A, Roes J. Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G. Immunity. 2000;12:201-10 pubmed
    ..The data demonstrate a critical role of the nonoxidative effector mechanisms of neutrophils in host immunity and immunopathology and identify elastase and cathepsin G as effectors in the endotoxic shock cascade downstream of TNFalpha. ..
  19. Frazziano G, Moreno L, Moral Sanz J, Menendez C, Escolano L, Gonzalez C, et al. Neutral sphingomyelinase, NADPH oxidase and reactive oxygen species. Role in acute hypoxic pulmonary vasoconstriction. J Cell Physiol. 2011;226:2633-40 pubmed publisher
    ..Exogenous ceramide increased ROS production in a PKC? sensitive manner. We propose an integrated signalling pathway for HPV which includes nSMase-PKC?-NADPH oxidase as a necessary step required for ROS production and vasoconstriction. ..
  20. Mardiney M, Jackson S, Spratt S, Li F, Holland S, Malech H. Enhanced host defense after gene transfer in the murine p47phox-deficient model of chronic granulomatous disease. Blood. 1997;89:2268-75 pubmed
    The p47phox-/- mouse exhibits a phenotype similar to that of human chronic granulomatous disease (CGD) and, thus, is an excellent model for the study of gene transfer technology...
  21. Syed I, Kyathanahalli C, Jayaram B, Govind S, Rhodes C, Kowluru R, et al. Increased phagocyte-like NADPH oxidase and ROS generation in type 2 diabetic ZDF rat and human islets: role of Rac1-JNK1/2 signaling pathway in mitochondrial dysregulation in the diabetic islet. Diabetes. 2011;60:2843-52 pubmed publisher
    ..We provide the first in vitro and in vivo evidence in support of an accelerated Rac1-Nox-ROS-JNK1/2 signaling pathway in the islet ?-cell leading to the onset of mitochondrial dysregulation in diabetes. ..
  22. Sharma N, Rabeler B, Zheng H, Raichlin E, Patel K. Exercise Training Attenuates Upregulation of p47(phox) and p67(phox) in Hearts of Diabetic Rats. Oxid Med Cell Longev. 2016;2016:5868913 pubmed publisher
  23. Becanovic K, Jagodic M, Sheng J, Dahlman I, Aboul Enein F, Wallstrom E, et al. Advanced intercross line mapping of Eae5 reveals Ncf-1 and CLDN4 as candidate genes for experimental autoimmune encephalomyelitis. J Immunol. 2006;176:6055-64 pubmed
    ..In conclusion, our data strongly suggests Ncf-1 to be a gene shared between two organ-specific inflammatory diseases with a possible contribution by CLDN4 in encephalomyelitis. ..
  24. Becker S, Reinehr R, Graf D, vom Dahl S, Haussinger D. Hydrophobic bile salts induce hepatocyte shrinkage via NADPH oxidase activation. Cell Physiol Biochem. 2007;19:89-98 pubmed
    ..In addition, cell shrinkage induced by proapoptotic bile salts may augment apoptosis by increasing protein breakdown and induction of cholestasis. ..
  25. Leto T, Adams A, de Mendez I. Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets. Proc Natl Acad Sci U S A. 1994;91:10650-4 pubmed
    ..We propose a model in which the oxidase complex assembles through a mechanism involving SH3 domains of both cytosolic proteins and cognate proline-rich targets in other oxidase components. ..
  26. Soe N, Sowden M, Baskaran P, Smolock E, Kim Y, Nigro P, et al. Cyclophilin A is required for angiotensin II-induced p47phox translocation to caveolae in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 2013;33:2147-53 pubmed publisher
    ..that CyPA regulates AngII-induced ROS generation by promoting translocation of NADPH oxidase cytosolic subunit p47phox to caveolae of the plasma membrane...
  27. Li Y, Wang S. Glycated albumin activates NADPH oxidase in rat mesangial cells through up-regulation of p47phox. Biochem Biophys Res Commun. 2010;397:5-11 pubmed publisher
    ..Moreover, glycated albumin treatment stimulated the expression and phosphorylation of p47phox, one of the cytosolic regulatory subunits of the NADPH oxidase...
  28. del Campo M, Antonell A, Magano L, Muñoz F, Flores R, Bayés M, et al. Hemizygosity at the NCF1 gene in patients with Williams-Beuren syndrome decreases their risk of hypertension. Am J Hum Genet. 2006;78:533-42 pubmed
    ..Deletion breakpoints were precisely defined and were found to result in variability at two genes, NCF1 and GTF2IRD2...
  29. Lv P, Miao S, Shu Y, Dong L, Liu G, Xie X, et al. Phosphorylation of smooth muscle 22? facilitates angiotensin II-induced ROS production via activation of the PKC?-P47phox axis through release of PKC? and actin dynamics and is associated with hypertrophy and hyperplasia of vascular smooth muscle cel. Circ Res. 2012;111:697-707 pubmed publisher
    ..However, the mechanisms by which SM22? controls ROS production have not been characterized...
  30. Balteau M, Tajeddine N, de Meester C, Ginion A, Des Rosiers C, Brady N, et al. NADPH oxidase activation by hyperglycaemia in cardiomyocytes is independent of glucose metabolism but requires SGLT1. Cardiovasc Res. 2011;92:237-46 pubmed publisher
    ..HG exposure activated Rac1GTP and induced p47phox translocation to the plasma membrane, resulting in NADPH oxidase (NOX2) activation, increased ROS production, ..
  31. Serezani C, Aronoff D, Jancar S, Mancuso P, Peters Golden M. Leukotrienes enhance the bactericidal activity of alveolar macrophages against Klebsiella pneumoniae through the activation of NADPH oxidase. Blood. 2005;106:1067-75 pubmed
    ..Both classes of LTs elicited translocation of p47phox to the cell membrane, and LTB4 induced phosphorylation of p47phox in a manner dependent on protein kinase C-delta ..
  32. Goldmann O, von Köckritz Blickwede M, Höltje C, Chhatwal G, Geffers R, Medina E. Transcriptome analysis of murine macrophages in response to infection with Streptococcus pyogenes reveals an unusual activation program. Infect Immun. 2007;75:4148-57 pubmed
    ..pyogenes is mediated by phagocyte oxidase, as p47phox was up-regulated in infected cells. Indeed, the in vivo and in vitro killing of S...
  33. Olofsson P, Nerstedt A, Hultqvist M, Nilsson E, Andersson S, Bergelin A, et al. Arthritis suppression by NADPH activation operates through an interferon-beta pathway. BMC Biol. 2007;5:19 pubmed
    ..the activating component of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex, neutrophil cytosolic factor 1 (NCF1), has previously been identified as a regulator of arthritis severity in mice and rats...
  34. Bignell E, Negrete Urtasun S, Calcagno A, Arst H, Rogers T, Haynes K. Virulence comparisons of Aspergillus nidulans mutants are confounded by the inflammatory response of p47phox-/- mice. Infect Immun. 2005;73:5204-7 pubmed
    ..This demonstrates that p47(phox)(-/-) murine immunity contributes significantly to A. nidulans lethality. These data have wider implications for microbial virulence studies with p47(phox)(-/-) mice. ..
  35. Jackson S, Gallin J, Holland S. The p47phox mouse knock-out model of chronic granulomatous disease. J Exp Med. 1995;182:751-8 pubmed
    ..We have created a mouse model of CGD by targeted disruption of p47phox, one of the genes in which mutations cause human CGD...
  36. Tung N, Sun K, Fan J, Shoyama Y, Han J. Oregonin from the Bark of Alnus japonica restrained ischemia-reperfusion-induced mesentery oxidative stress by inhibiting NADPH oxidase activation. Microcirculation. 2014;21:688-95 pubmed publisher
    ..The results suggested that ORG restrained I/R-induced ROS production, which might be correlated with its inhibitive effect on NADPH activation. ..
  37. Volpp B, Lin Y. In vitro molecular reconstitution of the respiratory burst in B lymphoblasts from p47-phox-deficient chronic granulomatous disease. J Clin Invest. 1993;91:201-7 pubmed
    ..Thus, B lymphoblasts provide an excellent model system for studies of the NADPH oxidase, for expression of functional recombinant forms of oxidase components, and for initial experimental approaches to genetic reconstitution in CGD. ..
  38. Winiarska K, Dzik J, Labudda M, Focht D, Sierakowski B, Owczarek A, et al. Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase. J Pineal Res. 2016;60:109-17 pubmed publisher
  39. Kan H, Hu W, Wang Y, Wu W, Yin Y, Liang Y, et al. NADPH oxidase-derived production of reactive oxygen species is involved in learning and memory impairments in 16-month-old female rats. Mol Med Rep. 2015;12:4546-4553 pubmed publisher
    ..of superoxide dismutase (SOD), level of malondialdehyde (MDA), production of ROS and expression levels of NOX2, p47phox, Ras?related C3 botulinum toxin substrate 1 (RAC1) and protein kinase C ? (PKC?) were investigated in the cortex ..
  40. Cooper A, Segal B, Frank A, Holland S, Orme I. Transient loss of resistance to pulmonary tuberculosis in p47(phox-/-) mice. Infect Immun. 2000;68:1231-4 pubmed
    ..This may be a consequence of increased tissue damage due to more rapid bacterial growth or may reflect a role for superoxide in controlling inflammation. ..
  41. Mirshafiey A, Mohsenzadegan M. The role of reactive oxygen species in immunopathogenesis of rheumatoid arthritis. Iran J Allergy Asthma Immunol. 2008;7:195-202 pubmed publisher
    ..The prevailing hypothesis that ROS promote inflammation was recently challenged when polymorphisms in Neutrophil cytosolic factor 1(Ncf1), that decrease oxidative burst, were shown to increase disease severity in mouse and rat ..
  42. Lian S, Xia Y, Khoi P, Ung T, Yoon H, Kim N, et al. Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-кB, and AP-1 pathways in human endothelial cells. Toxicology. 2015;338:104-16 pubmed publisher
    ..These results demonstrated that Cd induces MMP-9 expression via ROS-dependent EGFR->Erk1/2, JNK1/2->AP-1 and EGFR->Akt->NF-κB signaling pathways and, in turn, stimulates invasiveness in human endothelial cells. ..
  43. Ansari M, Roberts K, Scheff S. A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma. Free Radic Biol Med. 2014;77:21-9 pubmed publisher
    ..The initial 12 h post-TBI are very important as a possible window of opportunity to interrupt SIC. It may be important to selectively target the translocation of cytosolic subunits for the modulation of NOX function. ..
  44. Reeves E, Lu H, Jacobs H, Messina C, Bolsover S, Gabella G, et al. Killing activity of neutrophils is mediated through activation of proteases by K+ flux. Nature. 2002;416:291-7 pubmed
    ..We show that it is the proteases, thus activated, that are primarily responsible for the destruction of the bacteria. ..
  45. Lavigne M, Malech H, Holland S, Leto T. Genetic requirement of p47phox for superoxide production by murine microglia. FASEB J. 2001;15:285-7 pubmed
    ..Here, we provide genetic evidence that p47phox, an essential component of the phagocyte NADPH oxidase, is required for superoxide anion release from microglia...
  46. Distasi M, Unthank J, Miller S. Nox2 and p47(phox) modulate compensatory growth of primary collateral arteries. Am J Physiol Heart Circ Physiol. 2014;306:H1435-43 pubmed publisher
    ..These results have important implications for the use of antioxidants and the development of therapies for peripheral arterial disease. ..
  47. Rintisch C, Forster M, Holmdahl R. Detection of arthritis-susceptibility loci, including Ncf1, and variable effects of the major histocompatibility complex region depending on genetic background in rats. Arthritis Rheum. 2009;60:419-27 pubmed publisher
    ..A congenic fragment containing Ncf1 along with congenic fragments containing the strongest remaining loci, Pia5/Cia3 and Pia7/Cia13 on chromosome 4, ..
  48. Cao X, Dai X, Parker L, Kreulen D. Differential regulation of NADPH oxidase in sympathetic and sensory Ganglia in deoxycorticosterone acetate salt hypertension. Hypertension. 2007;50:663-71 pubmed
    ..This differential regulation may contribute to unbalanced vasomotor control and enhanced vasoconstriction in the splanchnic circulation. ..
  49. Reinehr R, Becker S, Keitel V, Eberle A, Grether Beck S, Haussinger D. Bile salt-induced apoptosis involves NADPH oxidase isoform activation. Gastroenterology. 2005;129:2009-31 pubmed
    ..TLCS induced a rapid serine phosphorylation of the regulatory subunit p47phox, which was sensitive to inhibition of sphingomyelinase and protein kinase Czeta (PKCzeta)...
  50. Jialal I, Devaraj S. Antisense to protein kinase C-alpha and p47phox attenuates the pro-inflammatory effects of human C-reactive protein in macrophages of biobreeding diabetic rats. Diab Vasc Dis Res. 2012;9:315-9 pubmed
    ..spontaneously diabetic biobreeding (BB) rats and increased the MO activity of protein kinase C-alpha (PKC-?) and p47phox. In this report, we tested the effects of molecular inhibition of CRP effects on MO activity using antisense ..
  51. Hahn N, Meischl C, Wijnker P, Musters R, Fornerod M, Janssen H, et al. NOX2, p22phox and p47phox are targeted to the nuclear pore complex in ischemic cardiomyocytes colocalizing with local reactive oxygen species. Cell Physiol Biochem. 2011;27:471-8 pubmed publisher
    ..g. under ischemia or high concentrations of homocysteine, is an important step in the induction of apoptosis in these cells. Here this ischemia-induced nuclear targeting and activation of NOX2 was specified in cardiomyocytes...
  52. Segal B, Kuhns D, Ding L, Gallin J, Holland S. Thioglycollate peritonitis in mice lacking C5, 5-lipoxygenase, or p47(phox): complement, leukotrienes, and reactive oxidants in acute inflammation. J Leukoc Biol. 2002;71:410-6 pubmed
    ..These data suggest that LTB(4) and C5a have separate but overlapping roles in thioglycollate-elicited peritonitis, and at least the leukotriene component is, in turn, regulated by reactive oxidants...
  53. Meng H, Griffiths M, Remmers E, Kawahito Y, Li W, Neisa R, et al. Identification of two novel female-specific non-major histocompatibility complex loci regulating collagen-induced arthritis severity and chronicity, and evidence of epistasis. Arthritis Rheum. 2004;50:2695-705 pubmed
    ..Sequencing analyses of the Cia25 candidate gene Ncf1 revealed polymorphisms between DA and ACI. The previously identified locus, Cia10, was found to be male-specific...
  54. Mitchison N, Harbord M, Hankin A, Roes J. Conditional haploinsufficiency of NCF1 (encoding p47(phox)), a signaling gene with a heterozygous phenotype potentially subject to natural selection. Immunol Lett. 2005;97:63-7 pubmed
    ..Searching for such a condition, we have re-examined the phenotype of mice +/- for the NCF1 gene encoding p47(phox) and humans +/- for NCF1 and NCF2 using a procedure that allowed the respiratory burst of ..
  55. Yu J, Lim J, Kim K, Morio T, Kim H. NADPH oxidase and apoptosis in cerulein-stimulated pancreatic acinar AR42J cells. Free Radic Biol Med. 2005;39:590-602 pubmed
    ..These results indicate that NADPH oxidase may mediate ROS-induced apoptosis in pancreatic acinar cells in a Ca(2+)-dependent manner. ..
  56. Fujita T, Hirooka K, Nakamura T, Itano T, Nishiyama A, Nagai Y, et al. Neuroprotective effects of angiotensin II type 1 receptor (AT1-R) blocker via modulating AT1-R signaling and decreased extracellular glutamate levels. Invest Ophthalmol Vis Sci. 2012;53:4099-110 pubmed publisher
    ..To investigate the mechanism of the neuroprotective effects of the angiotensin II type 1 receptor (AT1-R) blocker against retinal ischemia-reperfusion injury in the rat...
  57. Chen J, Xia C, Wang J, Jiang M, Zhang H, Zhang C, et al. The effect of orexin-A on cardiac dysfunction mediated by NADPH oxidase-derived superoxide anion in ventrolateral medulla. PLoS ONE. 2013;8:e69840 pubmed publisher
    ..double immunofluorescent staining indicated that OX1R positive cells and NAD(P)H oxidative subunit gp91phox or p47phox-immunoreactive (IR) cells were co-localized in RVLM...
  58. Schiavone S, Jaquet V, Sorce S, Dubois Dauphin M, Hultqvist M, Bäckdahl L, et al. NADPH oxidase elevations in pyramidal neurons drive psychosocial stress-induced neuropathology. Transl Psychiatry. 2012;2:e111 pubmed publisher
    ..Finally, at least at early stages, pharmacological targeting of NOX2 activity might reverse behavioral alterations. ..
  59. Jackson S, Malech H, Kozak C, Lomax K, Gallin J, Holland S. Cloning and functional expression of the mouse homologue of p47phox. Immunogenetics. 1994;39:272-5 pubmed
  60. Hsich E, Segal B, Pagano P, Rey F, Paigen B, Deleonardis J, et al. Vascular effects following homozygous disruption of p47(phox) : An essential component of NADPH oxidase. Circulation. 2000;101:1234-6 pubmed
    ..However, the absence of a functional oxidase does not significantly affect the progression of atherosclerosis in the standard mouse apoE -/- model, nor does it significantly influence basal blood pressure. ..
  61. Volpp B, Nauseef W, Donelson J, Moser D, Clark R. Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase. Proc Natl Acad Sci U S A. 1989;86:7195-9 pubmed
    ..These structural features are pertinent to proposed functional roles of the protein in the respiratory burst oxidase. ..
  62. Li Y, Zhao W, Yu X, Li F, Liu Z, Li L, et al. Activation of p47phox as a Mechanism of Bupivacaine-Induced Burst Production of Reactive Oxygen Species and Neural Toxicity. Oxid Med Cell Longev. 2017;2017:8539026 pubmed publisher
    ..important sources of ROS in the nervous system, and its activation requires the membrane translocation of subunit p47phox. However, the role of p47phox in bupivacaine-induced neurotoxicity has not been explored...