Gene Symbol: Mbd3
Description: methyl-CpG binding domain protein 3
Alias: methyl-CpG-binding domain protein 3
Species: rat
Products:     Mbd3

Top Publications

  1. Ghoshal K, Li X, Datta J, Bai S, Pogribny I, Pogribny M, et al. A folate- and methyl-deficient diet alters the expression of DNA methyltransferases and methyl CpG binding proteins involved in epigenetic gene silencing in livers of F344 rats. J Nutr. 2006;136:1522-7 pubmed
    ..These results demonstrate that a combined folate and methyl deficiency alters components of the DNA methylation machinery by both transcriptional and posttranscriptional mechanisms during early stages of hepatocarcinogenesis. ..
  2. Basta J, Rauchman M. The nucleosome remodeling and deacetylase complex in development and disease. Transl Res. 2015;165:36-47 pubmed publisher
    ..Recent genetic and biochemical studies have elucidated the mechanisms of NuRD action in modifying the chromatin landscape. These advances have the potential to lead to new therapeutic approaches to birth defects and cancer. ..
  3. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998;18:6538-47 pubmed
    ..We describe here three novel human and mouse proteins (MBD2, MBD3, and MBD4) that contain the methyl-CpG binding domain. MBD2 and MBD4 bind specifically to methylated DNA in vitro...
  4. Kantor B, Makedonski K, Shemer R, Razin A. Expression and localization of components of the histone deacetylases multiprotein repressory complexes in the mouse preimplantation embryo. Gene Expr Patterns. 2003;3:697-702 pubmed
    ..Proteins containing methyl binding domains (MBDs) are major components of these complexes. MBD3 is a component of the HDAC associated chromatin remodeling complex Mi2/NuRD...
  5. Kaji K, Caballero I, MacLeod R, Nichols J, Wilson V, Hendrich B. The NuRD component Mbd3 is required for pluripotency of embryonic stem cells. Nat Cell Biol. 2006;8:285-92 pubmed
    ..Embryonic stem cells lacking Mbd3 - a component of the nucleosome remodelling and histone deacetylation (NuRD) complex - were viable but failed to ..
  6. Francis J, Jung B, Zhang G, Cheng J, Ho W, Burnham W, et al. Kindling induces the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus. Neuroscience. 2002;113:79-87 pubmed
    ..the gene expression responses of a family of methyl CpG-binding domain-containing factors (MeCP2, MBD1, MBD2, and MBD3) in the hippocampus of electrically kindled rats...
  7. Jung B, Zhang G, Ho W, Francis J, Eubanks J. Transient forebrain ischemia alters the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus. Neuroscience. 2002;115:515-24 pubmed
    ..This delayed pattern of induction is in contrast to the responses of MBD2 and MBD3. Both MBD2 and MBD3 display significant changes in expression at early times following reperfusion, although their ..
  8. Jung B, Zhang G, Nitsch R, Trogadis J, Nag S, Eubanks J. Differential expression of methyl CpG-binding domain containing factor MBD3 in the developing and adult rat brain. J Neurobiol. 2003;55:220-32 pubmed
    Using subtractive hybridization screening, the methyl CpG-binding domain containing protein MBD3 was identified as being more prevalently expressed in the embryonic brain than in the adult...
  9. Datta J, Majumder S, Bai S, Ghoshal K, Kutay H, Smith D, et al. Physical and functional interaction of DNA methyltransferase 3A with Mbd3 and Brg1 in mouse lymphosarcoma cells. Cancer Res. 2005;65:10891-900 pubmed
    ..tandem mass spectrometry analysis of Dnmt3a-associated polypeptides identified the methyl CpG binding protein Mbd3, histone deacetylase 1(Hdac1), and components of Brg1 complex (Brg1, Baf155, and Baf57) in the purified preparation...

More Information


  1. Ruddock D Cruz N, Xue J, Wilson K, Heffernan C, Prashadkumar S, Cooney M, et al. Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development. Mol Reprod Dev. 2008;75:48-59 pubmed
    ..MBD1 localization is consistent with a novel role during MZT for both species. MBD3, known to play a crucial role in murine embryogenesis, was highly localized to the nucleus before and after, but ..
  2. Ma P, Lin S, Bartolomei M, Schultz R. Metastasis tumor antigen 2 (MTA2) is involved in proper imprinted expression of H19 and Peg3 during mouse preimplantation development. Biol Reprod. 2010;83:1027-35 pubmed publisher
    ..result in a decrease in abundance of other NuRD components, including methyl-binding-CpG-binding domain protein 3 (MBD3), histone deacetylases 1 and 2 (HDACs 1 and 2), and chromodomain helicase DNA-binding protein 4 (CHD4)...
  3. Kou C, Lau S, Au K, Leung P, Chim S, Fung K, et al. Epigenetic regulation of neonatal cardiomyocytes differentiation. Biochem Biophys Res Commun. 2010;400:278-83 pubmed publisher
    ..Therefore, we suggest that a hypermethylated genome and a more compact chromatin structure are formed during terminal differentiation of cardiomyocytes. ..
  4. Tang W, Morey L, Cheung Y, Birch L, Prins G, Ho S. Neonatal exposure to estradiol/bisphenol A alters promoter methylation and expression of Nsbp1 and Hpcal1 genes and transcriptional programs of Dnmt3a/b and Mbd2/4 in the rat prostate gland throughout life. Endocrinology. 2012;153:42-55 pubmed publisher
    ..We speculate that the distinctly different fate of early-life epigenetic marks during adulthood reflects the complex nature of lifelong editing of early-life epigenetic reprogramming. ..
  5. Cramer J, Scarsdale J, Walavalkar N, Buchwald W, Ginder G, Williams D. Probing the dynamic distribution of bound states for methylcytosine-binding domains on DNA. J Biol Chem. 2014;289:1294-302 pubmed publisher
    Although highly homologous to other methylcytosine-binding domain (MBD) proteins, MBD3 does not selectively bind methylated DNA, and thus the functional role of MBD3 remains in question...
  6. Yamada T, Yang Y, Hemberg M, Yoshida T, Cho H, Murphy J, et al. Promoter decommissioning by the NuRD chromatin remodeling complex triggers synaptic connectivity in the mammalian brain. Neuron. 2014;83:122-34 pubmed publisher
    ..Our findings define NuRD-dependent promoter decommissioning as a developmentally regulated programming mechanism that drives synaptic connectivity in the mammalian brain...