Masp2

Summary

Gene Symbol: Masp2
Description: mannan-binding lectin serine peptidase 2
Alias: MASP-2, MAp19, mannan-binding lectin serine protease 2, MBL-associated serine protease 2, mannose binding lectin-associated serine protease-2, mannose-binding protein-associated serine protease 2
Species: rat
Products:     Masp2

Top Publications

  1. Girija U, Dodds A, Roscher S, Reid K, Wallis R. Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins. J Immunol. 2007;179:455-62 pubmed
    ..Equivalent binding sites and comparable activation kinetics of MASP-2 suggest that complement activation by ficolins and MBLs proceeds by analogous mechanisms. ..
  2. Feinberg H, Uitdehaag J, Davies J, Wallis R, Drickamer K, Weis W. Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2. EMBO J. 2003;22:2348-59 pubmed
    ..A model of the full six-domain structure and its interaction with MBPs suggests mechanisms by which binding to a target cell transmits conformational changes from MBP to MASP that allow activation of its protease activity. ..
  3. Chen C, Wallis R. Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases. J Biol Chem. 2004;279:26058-65 pubmed
    ..MASP-1 cleaves C2 almost as efficiently as MASP-2 does, but it does not cleave C4. Thus MASP-1 probably enhances complement activation triggered by MBP.MASP-2 complexes, but it cannot initiate activation itself. ..
  4. Wallis R, Dodd R. Interaction of mannose-binding protein with associated serine proteases: effects of naturally occurring mutations. J Biol Chem. 2000;275:30962-9 pubmed
    ..Analysis of MASP binding by rat MBP containing naturally occurring mutations equivalent to those associated with human immunodeficiency indicates that binding to both truncated MASP-1 and MASP-2 proteins is defective in such mutants. ..
  5. Stover C, Thiel S, Thelen M, Lynch N, Vorup Jensen T, Jensenius J, et al. Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene. J Immunol. 1999;162:3481-90 pubmed
    ..In this study, we present the primary structure of this novel MBL-associated plasma protein of 19 kDa, MAp19, and demonstrate that MAp19 and MASP-2 are encoded by two different mRNA species generated by alternative splicing/..
  6. Stover C, Lynch N, Hanson S, Windbichler M, Gregory S, Schwaeble W. Organization of the MASP2 locus and its expression profile in mouse and rat. Mamm Genome. 2004;15:887-900 pubmed
    The mouse, rat, and human MASP2 loci are situated on syntenic chromosome regions and are highly conserved...
  7. Matsushita M, Thiel S, Jensenius J, Terai I, Fujita T. Proteolytic activities of two types of mannose-binding lectin-associated serine protease. J Immunol. 2000;165:2637-42 pubmed
    ..serine protease (MASP-1 and MASP-2) and a truncated protein of MASP-2 (small MBL-associated protein; sMAP or MAp19) are complexed with MBL...
  8. Nan R, Furze C, Wright D, Gor J, Wallis R, Perkins S. Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation. Structure. 2017;25:364-375 pubmed publisher
    ..We conclude that MASP-1 and MASP-2 are flexible at multiple sites and that this flexibility may permit both intra- and inter-complex activation. ..
  9. Cseh S, Vera L, Matsushita M, Fujita T, Arlaud G, Thielens N. Characterization of the interaction between L-ficolin/p35 and mannan-binding lectin-associated serine proteases-1 and -2. J Immunol. 2002;169:5735-43 pubmed
    ..with mannan-binding lectin (MBL)-associated serine proteases (MASP-1 and -2) and MBL-associated protein 19 (MAp19) in serum and forms complexes able to activate complement...

More Information

Publications18

  1. Stover C, Thiel S, Lynch N, Schwaeble W. The rat and mouse homologues of MASP-2 and MAp19, components of the lectin activation pathway of complement. J Immunol. 1999;163:6848-59 pubmed
    ..activation, a serine protease of 76 kDa, termed MASP-2, and a MASP-2 related plasma protein of 19 kDa, termed MAp19. Upon activation of the MBL/MASPs/MAp19 complex, MASP-2 cleaves the fourth complement component C4, while the role ..
  2. Endo Y, Nakazawa N, Liu Y, Iwaki D, Takahashi M, Fujita T, et al. Carbohydrate-binding specificities of mouse ficolin A, a splicing variant of ficolin A and ficolin B and their complex formation with MASP-2 and sMAP. Immunogenetics. 2005;57:837-44 pubmed
    ..These results suggest that ficolin A and its variant function as recognition molecules of the lectin pathway, and ficolin B plays a distinct role through its unique carbohydrate-binding specificity. ..
  3. Wallis R, Lynch N, Roscher S, Reid K, Schwaeble W. Decoupling of carbohydrate binding and MASP-2 autoactivation in variant mannose-binding lectins associated with immunodeficiency. J Immunol. 2005;175:6846-51 pubmed
    ..Analogous molecular defects in human MBL probably combine to create the mutant phenotypes of immunodeficient individuals. ..
  4. Venkatraman Girija U, Furze C, Toth J, Schwaeble W, Mitchell D, Keeble A, et al. Engineering novel complement activity into a pulmonary surfactant protein. J Biol Chem. 2010;285:10546-52 pubmed publisher
    ..Thus, the active rather than the zymogen state is default in lectin.MASP complexes and must be inhibited through additional regions in circulating MBLs until triggered by pathogen recognition. ..
  5. Kidmose R, Laursen N, Dobó J, Kjaer T, Sirotkina S, Yatime L, et al. Structural basis for activation of the complement system by component C4 cleavage. Proc Natl Acad Sci U S A. 2012;109:15425-30 pubmed
    ..Our results provide a unified structural framework for understanding the early and essential step of C4 cleavage in the elimination of pathogens and altered self through two major pathways of complement activation. ..
  6. Møller Kristensen M, Thiel S, Sjoholm A, Matsushita M, Jensenius J. Cooperation between MASP-1 and MASP-2 in the generation of C3 convertase through the MBL pathway. Int Immunol. 2007;19:141-9 pubmed
  7. Thiel S, Vorup Jensen T, Stover C, Schwaeble W, Laursen S, Poulsen K, et al. A second serine protease associated with mannan-binding lectin that activates complement. Nature. 1997;386:506-10 pubmed
    ..Thus complement activation through MBL, like the classical pathway, involves two serine proteases and may antedate the development of the specific immune system of vertebrates. ..
  8. Ambrus G, Gal P, Kojima M, Szilágyi K, Balczer J, Antal J, et al. Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments. J Immunol. 2003;170:1374-82 pubmed
    ..Nevertheless, relative rates of reaction with alpha-2-macroglobulin and C1-inhibitor suggest that alpha-2-macroglobulin may be a significant physiological inhibitor of MASP-1. ..
  9. Wallis R, Dodds A, Mitchell D, Sim R, Reid K, Schwaeble W. Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway. J Biol Chem. 2007;282:7844-51 pubmed