Lrat

Summary

Gene Symbol: Lrat
Description: lecithin retinol acyltransferase
Alias: lecithin retinol acyltransferase, lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase), phosphatidylcholine--retinol O-acyltransferase
Species: rat
Products:     Lrat

Top Publications

  1. Kiser P, Golczak M, Maeda A, Palczewski K. Key enzymes of the retinoid (visual) cycle in vertebrate retina. Biochim Biophys Acta. 2012;1821:137-51 pubmed publisher
    ..This article is part of a Special Issue entitled: Retinoid and Lipid Metabolism. ..
  2. Blaner W, Van Bennekum A, Brouwer A, Hendriks H. Distribution of lecithin-retinol acyltransferase activity in different types of rat liver cells and subcellular fractions. FEBS Lett. 1990;274:89-92 pubmed
    It is now well documented that lecithin-retinol acyltransferase (LRAT) is the physiologically important enzyme activity involved in the esterification of retinol in the liver...
  3. Daiger S, Sullivan L, Bowne S. Genes and mutations causing retinitis pigmentosa. Clin Genet. 2013;84:132-41 pubmed publisher
    ..In this review, we summarize the current approaches to gene discovery and mutation detection for RP, and indicate pitfalls and unsolved problems. Similar considerations apply to other forms of inherited retinal disease. ..
  4. Wu L, Ross A. Acidic retinoids synergize with vitamin A to enhance retinol uptake and STRA6, LRAT, and CYP26B1 expression in neonatal lung. J Lipid Res. 2010;51:378-87 pubmed publisher
    ..the period of absorption correlated with increased expression of both STRA6 (retinol-binding protein receptor) and LRAT (retinol esterification), while a reduction in RE after 12 h in Am580-treated, VA-supplemented rats correlated with ..
  5. Nagatsuma K, Hayashi Y, Hano H, Sagara H, Murakami K, Saito M, et al. Lecithin: retinol acyltransferase protein is distributed in both hepatic stellate cells and endothelial cells of normal rodent and human liver. Liver Int. 2009;29:47-54 pubmed publisher
    ..The present study was undertaken to examine whether lecithin:retinol acyltransferase (LRAT), the physiological retinol esterification enzyme of the liver, is a potential and relevant tissue marker for HSC...
  6. Thompson D, Li Y, McHenry C, Carlson T, Ding X, Sieving P, et al. Mutations in the gene encoding lecithin retinol acyltransferase are associated with early-onset severe retinal dystrophy. Nat Genet. 2001;28:123-4 pubmed
    ..1). The first of these reactions is catalyzed by lecithin retinol acyltransferase (LRAT); (ref. 2)...
  7. Zolfaghari R, Ross A. Lecithin:retinol acyltransferase from mouse and rat liver. CDNA cloning and liver-specific regulation by dietary vitamin a and retinoic acid. J Lipid Res. 2000;41:2024-34 pubmed
    Lecithin:retinol acyltransferase (LRAT), present in microsomes, catalyzes the transfer of the sn-1 fatty acid of phosphatidylcholine to retinol bound to a cellular retinol-binding protein...
  8. Zolfaghari R, Wang Y, Chen Q, Sancher A, Ross A. Cloning and molecular expression analysis of large and small lecithin:retinol acyltransferase mRNAs in the liver and other tissues of adult rats. Biochem J. 2002;368:621-31 pubmed
    ..the most abundant form of vitamin A (retinol), is synthesized by the enzyme lecithin:retinol acyltransferase (LRAT). Previously, we cloned a 2.5 kb LRAT cDNA from rodent liver which codes for functional LRAT activity...
  9. Wright A, Chakarova C, Abd El Aziz M, Bhattacharya S. Photoreceptor degeneration: genetic and mechanistic dissection of a complex trait. Nat Rev Genet. 2010;11:273-84 pubmed publisher
    ..Although genetic and mechanistic diversity creates challenges for therapy, some approaches--particularly gene-replacement therapy--are showing considerable promise. ..

More Information

Publications21

  1. Alapatt P, Guo F, Komanetsky S, Wang S, Cai J, Sargsyan A, et al. Liver retinol transporter and receptor for serum retinol-binding protein (RBP4). J Biol Chem. 2013;288:1250-65 pubmed publisher
    ..In addition, expression of RBPR2 in liver and fat suggests a possible role in mediating established metabolic actions of RBP4 in those tissues. ..
  2. Randolph R, Ross A. Vitamin A status regulates hepatic lecithin: retinol acyltransferase activity in rats. J Biol Chem. 1991;266:16453-7 pubmed
    The activity of lecithin:retinol acyltransferase (LRAT) was determined in microsomes from the liver and small intestine of rats with differing vitamin A status...
  3. Cia D, Bonhomme B, Azaïs Braesco V, Cluzel J, Doly M. Uptake and esterification of vitamin A by RCS rat retinal pigment epithelial cells in primary culture. Vision Res. 2004;44:247-55 pubmed
    ..Our results provide evidence of a functional retinol esterifying enzyme in cultured RCS RPE cells and suggest that CRBP-I could play a role in the uptake and esterification of ROL in the RPE cells. ..
  4. Zolfaghari R, Ross A. An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene. Arch Biochem Biophys. 2009;489:1-9 pubmed publisher
    Lecithin:retinol acyltransferase (LRAT) is essential for vitamin A storage. Nuclear run-on assays demonstrated transcriptional regulation of the Lrat gene in vivo by all-trans-retinoic acid (RA) and other retinoids. Analysis of a 2...
  5. Napoli J. Physiological insights into all-trans-retinoic acid biosynthesis. Biochim Biophys Acta. 2012;1821:152-67 pubmed publisher
    ..This article is part of a Special Issue entitled: Retinoid and Lipid Metabolism. ..
  6. Mondal M, Ruiz A, Bok D, Rando R. Lecithin retinol acyltransferase contains cysteine residues essential for catalysis. Biochemistry. 2000;39:5215-20 pubmed
    b>Lecithin retinol acyltransferase (LRAT) is an essential enzyme in vitamin A metabolism and mobilization...
  7. Kim Y, Wassef L, Hamberger L, Piantedosi R, Palczewski K, Blaner W, et al. Retinyl ester formation by lecithin: retinol acyltransferase is a key regulator of retinoid homeostasis in mouse embryogenesis. J Biol Chem. 2008;283:5611-21 pubmed
    ..We investigated the role of retinyl ester formation catalyzed by lecithin:retinol acyltransferase (LRAT) in regulating retinoid homeostasis during embryogenesis...
  8. Shimada T, Ross A, Muccio D, Brouillette W, Shealy Y. Regulation of hepatic lecithin:retinol acyltransferase activity by retinoic acid receptor-selective retinoids. Arch Biochem Biophys. 1997;344:220-7 pubmed
    The microsomal enzyme LRAT esterifies retinol and has been implicated in the hepatic storage of vitamin A...
  9. Takitani K, Miyazaki H, Fukunishi S, Takaya R, Yoden A, Higuchi K, et al. Altered expression of both ?-carotene 15,15' monooxygenase and lecithin:retinol acyltransferase in obese Zucker rats. J Nutr Sci Vitaminol (Tokyo). 2011;57:108-13 pubmed
    ..We examined the expression of genes for ?-carotene 15,15' monooxygenase (BCM), lecithin:retinol acyltransferase (LRAT), cellular retinol binding protein-I (CRBP-I), and cytochrome P450 26A1 (CYP26A1) in fa/fa rats and lean control ..
  10. Harrison E. Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids. Biochim Biophys Acta. 2012;1821:70-7 pubmed publisher
    ..Polymorphisms in genes for these proteins lead to individual variability in the metabolism and transport of vitamin A and carotenoids. This article is part of a Special Issue entitled Retinoid and Lipid Metabolism. ..
  11. Matsuura T, Ross A. Regulation of hepatic lecithin: retinol acyltransferase activity by retinoic acid. Arch Biochem Biophys. 1993;301:221-7 pubmed
    The activity of the hepatic enzyme lecithin: retinol acyltransferase (LRAT), thought to catalyze the esterification of retinol for storage, was previously shown to vary directly with the vitamin A (retinol) status of the rat [Randolph, R...
  12. MacDonald P, Ong D. Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine. J Biol Chem. 1988;263:12478-82 pubmed