Gene Symbol: Kif5b
Description: kinesin family member 5B
Alias: Khc, kinesin-1 heavy chain, UKHC, conventional kinesin heavy chain, ubiquitous kinesin heavy chain
Species: rat
Products:     Kif5b

Top Publications

  1. Cai D, Hoppe A, Swanson J, Verhey K. Kinesin-1 structural organization and conformational changes revealed by FRET stoichiometry in live cells. J Cell Biol. 2007;176:51-63 pubmed
    ..resonance energy transfer (FRET) stoichiometry to fluorescent protein (FP)-labeled kinesin heavy chain (KHC) and kinesin light chain (KLC) subunits in live cells, we studied the overall structural organization and ..
  2. Blasius T, Cai D, Jih G, Toret C, Verhey K. Two binding partners cooperate to activate the molecular motor Kinesin-1. J Cell Biol. 2007;176:11-7 pubmed
    ..We show that binding of JIP1 and FEZ1 to Kinesin-1 is sufficient to activate the motor for MT binding and motility. These results provide the first demonstration of the activation of a MT-based motor by cellular binding partners. ..
  3. Splinter D, Tanenbaum M, Lindqvist A, Jaarsma D, Flotho A, Yu K, et al. Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry. PLoS Biol. 2010;8:e1000350 pubmed publisher
  4. Satoh T. Rho GTPases in insulin-stimulated glucose uptake. Small Gtpases. 2014;5:e28102 pubmed publisher
    ..In addition, the role for another Rho family member TC10 and its regulatory mechanism in adipocyte insulin signaling are described. ..
  5. Fujita T, Maturana A, Ikuta J, Hamada J, Walchli S, Suzuki T, et al. Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells. Biochem Biophys Res Commun. 2007;361:605-10 pubmed
    ..Thus, our results suggest that the FEZ1/kinesin complex functions for the transport of mitochondria along microtubules toward the extending neurites in differentiating PC12 cells. ..
  6. Rahmati M, Gharakhanlou R, Movahedin M, Mowla S, Khazani A, Fouladvand M, et al. Treadmill training modifies KIF5B motor protein in the STZ-induced diabetic rat spinal cord and sciatic nerve. Arch Iran Med. 2015;18:94-101 pubmed publisher
    ..In the present study, we investigated the effect of regular treadmill exercise on KIF5B and Sunday Driver (SYD) mRNA levels in sensory and motor parts of spinal cord and KIF5B content in sciatic nerves ..
  7. Diefenbach R, Davis A, Miranda Saksena M, Fernandez M, Kelly B, Jones C, et al. The Basic Domain of Herpes Simplex Virus 1 pUS9 Recruits Kinesin-1 To Facilitate Egress from Neurons. J Virol. 2016;90:2102-11 pubmed publisher
    ..In this study, we have defined how a key viral protein plays a role in both axonal transport and spread of the virus from nerve cells to the skin. ..
  8. Pekkurnaz G, Trinidad J, Wang X, Kong D, Schwarz T. Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Cell. 2014;158:54-68 pubmed publisher
    ..Our findings suggest that, by dynamically regulating Milton GlcNAcylation, OGT tailors mitochondrial dynamics in neurons based on nutrient availability. ..
  9. Rivera J, Chu P, Lewis T, Arnold D. The role of Kif5B in axonal localization of Kv1 K(+) channels. Eur J Neurosci. 2007;25:136-46 pubmed
    Here we present evidence that the kinesin, Kif5B, is involved in the transportation and axonal targeting of Kv1 channels...

More Information


  1. Tigchelaar W, de Jong A, Bloks V, van Gilst W, de Boer R, Silljé H. Hypertrophy induced KIF5B controls mitochondrial localization and function in neonatal rat cardiomyocytes. J Mol Cell Cardiol. 2016;97:70-81 pubmed publisher
    ..we identified a gene encoding a motor protein implicated in mitochondrial localization, kinesin family member 5b (Kif5b), which was clearly elevated in PE stimulated NRVCs but not in IGF1 stimulated NRVCs...
  2. Mori T, Vale R, Tomishige M. How kinesin waits between steps. Nature. 2007;450:750-4 pubmed
    ..On the basis of these results, we suggest a model for how transitions in the ATPase cycle position the two kinesin heads and drive their hand-over-hand motion. ..
  3. Utton M, Noble W, Hill J, Anderton B, Hanger D. Molecular motors implicated in the axonal transport of tau and alpha-synuclein. J Cell Sci. 2005;118:4645-54 pubmed
  4. Ligon L, Tokito M, Finklestein J, Grossman F, Holzbaur E. A direct interaction between cytoplasmic dynein and kinesin I may coordinate motor activity. J Biol Chem. 2004;279:19201-8 pubmed
    ..There is also a vesicle population with both dynein and kinesin I bound that may be capable of bi-directional motility along cellular microtubules. ..
  5. Tomishige M, Stuurman N, Vale R. Single-molecule observations of neck linker conformational changes in the kinesin motor protein. Nat Struct Mol Biol. 2006;13:887-94 pubmed
    ..During ATP-driven motility, the neck linkers switch between these conformational states. These results support the notion that neck linker movements accompany the 'hand-over-hand' motion of the two motor domains. ..
  6. Yang J, Li T. The ciliary rootlet interacts with kinesin light chains and may provide a scaffold for kinesin-1 vesicular cargos. Exp Cell Res. 2005;309:379-89 pubmed
    ..These findings indicate that ciliary rootlets may provide a scaffold for kinesin-1 vesicular cargos and, thus, play a role in the intracellular transport in ciliated cells. ..
  7. Santarella R, Skiniotis G, Goldie K, Tittmann P, Gross H, Mandelkow E, et al. Surface-decoration of microtubules by human tau. J Mol Biol. 2004;339:539-53 pubmed
    ..Hence, it binds along, as well as across protofilaments. Nevertheless, even minute concentrations of tau have a strong stabilizing effect and effectively scavenge unpolymerized tubulin. ..
  8. Pigino G, Morfini G, Pelsman A, Mattson M, Brady S, Busciglio J. Alzheimer's presenilin 1 mutations impair kinesin-based axonal transport. J Neurosci. 2003;23:4499-508 pubmed
    ..These findings suggest that mutations in PS1 may compromise neuronal function by affecting GSK-3 activity and kinesin-I-based motility. ..
  9. Lalioti V, Vergarajauregui S, Tsuchiya Y, Hernandez Tiedra S, Sandoval I. Daxx functions as a scaffold of a protein assembly constituted by GLUT4, JNK1 and KIF5B. J Cell Physiol. 2009;218:416-26 pubmed publisher
    ..In addition, Daxx interacts with kinesin KIF5B through the 6xTPR domain of the kinesin light chain, a domain engaged in the grab hold of protein cargo by kinesin ..
  10. Gould R, Freund C, Palmer F, Knapp P, Huang J, Morrison H, et al. Messenger RNAs for kinesins and dynein are located in neural processes. Biol Bull. 1999;197:259-60 pubmed
  11. Ma H, Cai Q, Lu W, Sheng Z, Mochida S. KIF5B motor adaptor syntabulin maintains synaptic transmission in sympathetic neurons. J Neurosci. 2009;29:13019-29 pubmed publisher
    ..Our previous studies in developing hippocampal neurons revealed that syntabulin acts as a KIF5B motor adaptor and mediates anterograde transport of presynaptic cargoes and mitochondria, presynaptic assembly, ..
  12. Nath S, Bananis E, Sarkar S, Stockert R, Sperry A, Murray J, et al. Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver. Mol Biol Cell. 2007;18:1839-49 pubmed
    ..Studies with specific antibodies confirmed that dynein was not associated with these vesicles and that Kif5B and the minus-end kinesin Kifc1 mediated their plus- and minus-end motility, respectively...
  13. Cai Q, Pan P, Sheng Z. Syntabulin-kinesin-1 family member 5B-mediated axonal transport contributes to activity-dependent presynaptic assembly. J Neurosci. 2007;27:7284-96 pubmed
    ..Our previous studies established that syntabulin is an adaptor capable of conjoining the kinesin family member 5B (KIF5B) motor and syntaxin-1...
  14. Baptista F, Pinto M, Elvas F, Almeida R, Ambrósio A. Diabetes alters KIF1A and KIF5B motor proteins in the hippocampus. PLoS ONE. 2013;8:e65515 pubmed publisher
    ..We investigated the effect of diabetes (2 and 8 weeks duration) on KIF1A, KIF5B and dynein motor proteins, which are important for axonal transport, in the hippocampus...
  15. Kuribayashi J, Kitaoka Y, Munemasa Y, Ueno S. Kinesin-1 and degenerative changes in optic nerve axons in NMDA-induced neurotoxicity. Brain Res. 2010;1362:133-40 pubmed publisher
    ..Western blot analysis showed early elevation of kinesin-1 (KIF5B) protein levels in the retina 24 and 72h after NMDA injection...
  16. Liu M, Nadar V, Kozielski F, Kozlowska M, Yu W, Baas P. Kinesin-12, a mitotic microtubule-associated motor protein, impacts axonal growth, navigation, and branching. J Neurosci. 2010;30:14896-906 pubmed publisher
  17. Du W, Su Q, Chen Y, Zhu Y, Jiang D, Rong Y, et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell. 2016;37:326-336 pubmed publisher
    ..using an approach combining in vivo studies and in vitro reconstitution, we found that the kinesin motor protein KIF5B is required for autolysosome tubulation and that KIF5B drives autolysosome tubulation by pulling on the ..
  18. Twelvetrees A, Yuen E, Arancibia Carcamo I, Macaskill A, Rostaing P, Lumb M, et al. Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and disrupted by mutant huntingtin. Neuron. 2010;65:53-65 pubmed publisher
    ..Its disruption by mutant huntingtin may explain some of the defects in brain information processing occurring in Huntington's disease and provides a molecular target for therapeutic approaches. ..
  19. Zadeh A, Cheng Y, Xu H, Wong N, Wang Z, Goonasekara C, et al. Kif5b is an essential forward trafficking motor for the Kv1.5 cardiac potassium channel. J Physiol. 2009;587:4565-74 pubmed publisher
    We have investigated the role of the kinesin I isoform Kif5b in the trafficking of a cardiac voltage-gated potassium channel, Kv1.5. In Kv1...
  20. Tanaka Y, Kanai Y, Okada Y, Nonaka S, Takeda S, Harada A, et al. Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria. Cell. 1998;93:1147-58 pubmed
    ..5-11.5 days postcoitum. To analyze the significance of this conventional kinesin heavy chain in organelle transport, we studied the distribution of major organelles in the extraembryonic cells...
  21. Vignali G, Lizier C, Sprocati M, Sirtori C, Battaglia G, Navone F. Expression of neuronal kinesin heavy chain is developmentally regulated in the central nervous system of the rat. J Neurochem. 1997;69:1840-9 pubmed
  22. Barry J, Xu M, Gu Y, Dangel A, Jukkola P, Shrestha C, et al. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels. J Cell Sci. 2013;126:2027-41 pubmed publisher
    ..Our biochemical assays reveal that the high-affinity multimeric binding between the Kv3.1 T1 domain and KIF5B requires three basic residues in the KIF5B tail. Kv3...
  23. Kawano Y, Yoshimura T, Tsuboi D, Kawabata S, Kaneko Kawano T, Shirataki H, et al. CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation. Mol Cell Biol. 2005;25:9920-35 pubmed
    ..These results suggest that CRMP-2 transports the Sra-1/WAVE1 complex to axons in a kinesin-1-dependent manner and thereby regulates axon outgrowth and formation. ..
  24. Niclas J, Navone F, Hom Booher N, Vale R. Cloning and localization of a conventional kinesin motor expressed exclusively in neurons. Neuron. 1994;12:1059-72 pubmed
    ..motor has been thought to serve all cell types, we document here that neurons express a second conventional kinesin heavy chain (nKHC) that is 65% identical in amino acid sequence to the ubiquitously expressed kinesin heavy chain ..
  25. Loschi M, Leishman C, Berardone N, Boccaccio G. Dynein and kinesin regulate stress-granule and P-body dynamics. J Cell Sci. 2009;122:3973-82 pubmed publisher
    ..Conversely, impairment of anterograde transport by knockdown of kinesin-1 heavy chain (KIF5B) or kinesin light chain 1 (KLC1) delayed SG dissolution...
  26. Su Y, Ye M, Li L, Liu C, Pan J, Liu W, et al. KIF5B promotes the forward transport and axonal function of the voltage-gated sodium channel Nav1.8. J Neurosci. 2013;33:17884-96 pubmed publisher
    ..Here we report that KIF5B promotes the forward transport of Nav1...
  27. McDade J, Michele D. Membrane damage-induced vesicle-vesicle fusion of dysferlin-containing vesicles in muscle cells requires microtubules and kinesin. Hum Mol Genet. 2014;23:1677-86 pubmed publisher
    ..Our data indicate that dysferlin-containing vesicles move along microtubules via the kinesin motor KIF5B in muscle cells...
  28. Yang S, Xia C, Li S, Du L, Zhang L, Zhou R. Defective mitophagy driven by dysregulation of rheb and KIF5B contributes to mitochondrial reactive oxygen species (ROS)-induced nod-like receptor 3 (NLRP3) dependent proinflammatory response and aggravates lipotoxicity. Redox Biol. 2014;3:63-71 pubmed publisher
    ..In addition, sustained PA caused disassociation of kinesin family member 5B (KIF5B) from binding with mitochondria via Ca(2+)-dependent effects...
  29. Lin M, Sheng Z. Regulation of mitochondrial transport in neurons. Exp Cell Res. 2015;334:35-44 pubmed publisher
    ..The review focuses on microtubule (MT)-based mitochondrial trafficking and anchoring. Additional insight from different perspectives can be found in other in-depth reviews. ..
  30. Cho K, Cai Y, Yi H, Yeh A, Aslanukov A, Ferreira P. Association of the kinesin-binding domain of RanBP2 to KIF5B and KIF5C determines mitochondria localization and function. Traffic. 2007;8:1722-35 pubmed
    ..selectively in the central nervous system (CNS), and directly, with the ubiquitous and CNS-specific kinesins, KIF5B and KIF5C, respectively, but not with the highly homologous KIF5A...