Gene Symbol: Id1
Description: inhibitor of DNA binding 1, HLH protein
Alias: ID125A, Idb1, DNA-binding protein inhibitor ID-1, Inhibitor of DNA binding 1 helix-loop-helix protein (splice varaiation), Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation), inhibitor of DNA binding 1, dominant negative helix-loop-helix protein, inhibitor of differentiation 1
Species: rat
Products:     Id1

Top Publications

  1. Everly D, Mainou B, Raab Traub N. Induction of Id1 and Id3 by latent membrane protein 1 of Epstein-Barr virus and regulation of p27/Kip and cyclin-dependent kinase 2 in rodent fibroblast transformation. J Virol. 2004;78:13470-8 pubmed
    ..Expression of two members of the Id (inhibitor of differentiation) family of proteins, Id1 and Id3, was induced in the presence of LMP1 and confirmed by mRNA and protein in C33A and Rat-1 cells...
  2. Yu Y, Gao Y, Qin J, Kuang C, Song M, Yu S, et al. CCN1 promotes the differentiation of endothelial progenitor cells and reendothelialization in the early phase after vascular injury. Basic Res Cardiol. 2010;105:713-24 pubmed publisher vitro and 20 genes were found differentially expressed during CCN1-induced EPC differentiation, including Id1, Vegf-b, Vegf-c, Kdr, Igf-1, Ereg, Tgf, Mdk, Ptn, Timp2, etc...
  3. Nagata Y, Shoji W, Obinata M, Todokoro K. Phosphorylation of helix-loop-helix proteins ID1, ID2 and ID3. Biochem Biophys Res Commun. 1995;207:916-26 pubmed
    ..It has been noted that putative phosphorylation sites for various protein kinases exist in rat Id1, Id2 and Id3...
  4. Springhorn J, Singh K, Kelly R, Smith T. Posttranscriptional regulation of Id1 activity in cardiac muscle. Alternative splicing of novel Id1 transcript permits homodimerization. J Biol Chem. 1994;269:5132-6 pubmed
    ..Following the observation of a "doublet" band of 1.1 and 1.25 kilobases on Northern hybridizations of Id1 cDNA with mRNA isolated from both cardiac muscle and vascular smooth muscle cells, we identified and sequenced an ..
  5. Carroll M, Hamzeh M, Robaire B. Expression, localization, and regulation of inhibitor of DNA binding (Id) proteins in the rat epididymis. J Androl. 2006;27:212-24 pubmed
    ..The objectives of this study were to determine the mRNA expression and the immunocytolocalization of Id1, Id2, Id3, and Id4 in the epididymis of adult rats and to determine the Id3 protein expression profile in ..
  6. Kho C, Huggins G, Endege W, Hsieh C, Lee M, Haber E. Degradation of E2A proteins through a ubiquitin-conjugating enzyme, UbcE2A. J Biol Chem. 1997;272:3845-51 pubmed
    ..Finally, antisense UbcE2A reduces E12 degradation. By participating in the degradation of the E2A proteins, UbcE2A may regulate cell growth and differentiation. ..
  7. Chaudhary J, Sadler Riggleman I, Ague J, Skinner M. The helix-loop-helix inhibitor of differentiation (ID) proteins induce post-mitotic terminally differentiated Sertoli cells to re-enter the cell cycle and proliferate. Biol Reprod. 2005;72:1205-17 pubmed
    ..The four known helix-loop-helix ID proteins (i.e., Id1, Id2, Id3, and Id4) are considered dominant negative regulators of cellular differentiation pathways and act as ..
  8. Nagata Y, Todokoro K. Activation of helix-loop-helix proteins Id1, Id2 and Id3 during neural differentiation. Biochem Biophys Res Commun. 1994;199:1355-62 pubmed
    ..We have characterized three Id-related cDNAs, Id1, Id2 and Id3, isolated from nerve growth factor (NGF)-stimulated PC12 cells...
  9. Yang J, Li X, Al Lamki R, Southwood M, Zhao J, Lever A, et al. Smad-dependent and smad-independent induction of id1 by prostacyclin analogues inhibits proliferation of pulmonary artery smooth muscle cells in vitro and in vivo. Circ Res. 2010;107:252-62 pubmed publisher
    ..Mutations lead to reduced Smad1/5-driven expression of inhibitor of DNA binding protein 1 (Id1) and loss of the growth suppressive effects of BMPs...

More Information


  1. Chen J, Sun W, Zheng Y, Xiong H, Cai Y. Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells. J Neurosci Res. 2013;91:515-26 pubmed publisher
    ..was to determine whether BMP4 can regulate the survival of cochlear sensory epithelial cells through inhibitor of differentiation 1 (Id1) and the epidermal growth factor receptor (EGFR)...
  2. Bai G, Sheng N, Xie Z, Bian W, Yokota Y, Benezra R, et al. Id sustains Hes1 expression to inhibit precocious neurogenesis by releasing negative autoregulation of Hes1. Dev Cell. 2007;13:283-97 pubmed
    ..By contrast, Hes1 expression decreases, proneural gene expression expands, and neurogenesis occurs precociously in Id1;Id3 double knockout mice and in Id1-3 RNAi-electroporated chick embryos...
  3. Yamashita M, Ying S, Zhang G, Li C, Cheng S, Deng C, et al. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell. 2005;121:101-13 pubmed
    ..These results indicate that Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. ..
  4. Volpert O, Pili R, Sikder H, Nelius T, Zaichuk T, Morris C, et al. Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1. Cancer Cell. 2002;2:473-83 pubmed
    ..In order to identify downstream effectors of Id1 involved in the regulation of angiogenesis, we performed PCR-select subtractive hybridization on wild-type and Id1 ..
  5. Konishi H, Ogawa T, Nakagomi S, Inoue K, Tohyama M, Kiyama H. Id1, Id2 and Id3 are induced in rat melanotrophs of the pituitary gland by dopamine suppression under continuous stress. Neuroscience. 2010;169:1527-34 pubmed publisher
    ..RT-PCR, Western blotting and in situ hybridization confirmed the significant inductions of Id1, Id2 and Id3 in the IL of CS rats...
  6. Zheng W, Wang H, Xue L, Zhang Z, Tong T. Regulation of cellular senescence and p16(INK4a) expression by Id1 and E47 proteins in human diploid fibroblast. J Biol Chem. 2004;279:31524-32 pubmed
    b>Id1, a member of Id family of helix-loop-helix transcriptional regulatory proteins, is implicated in cellular senescence by repressing p16(INK4a) expression, but the mechanisms and cellular effects in human diploid fibroblasts remain ..
  7. Peng Y, Kang Q, Luo Q, Jiang W, Si W, Liu B, et al. Inhibitor of DNA binding/differentiation helix-loop-helix proteins mediate bone morphogenetic protein-induced osteoblast differentiation of mesenchymal stem cells. J Biol Chem. 2004;279:32941-9 pubmed
  8. Vinals F, Ventura F. Myogenin protein stability is decreased by BMP-2 through a mechanism implicating Id1. J Biol Chem. 2004;279:45766-72 pubmed
    ..Forced expression of Id1, either by transient transfection or under the control of an inducible system, causes degradation of myogenin in ..
  9. Bergonzini V, Delbue S, Wang J, Reiss K, Prisco M, Amini S, et al. HIV-Tat promotes cellular proliferation and inhibits NGF-induced differentiation through mechanisms involving Id1 regulation. Oncogene. 2004;23:7701-11 pubmed
    b>Id1 is a helix-loop-helix transcriptional factor that controls growth and survival of neuronal cells...
  10. Lin J, Chang S, Hsieh D, Lee C, Yu D. The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells. Oncol Rep. 2005;13:983-8 pubmed
    ..Our findings suggest that overproduction of the Id-1, MIF and GSTpi proteins and coinduction of mdr-1 play an important role in the development of acquired drug resistance to various drugs of prostate cancer cells. ..
  11. Wiercinska E, Wickert L, Denecke B, Said H, Hamzavi J, Gressner A, et al. Id1 is a critical mediator in TGF-beta-induced transdifferentiation of rat hepatic stellate cells. Hepatology. 2006;43:1032-41 pubmed
    ..Smad7-dependent mRNA expression profiles in HSCs was performed, resulting in the identification of the inhibitor of differentiation 1 (Id1) gene. Ectopic Smad7 expression in HSCs strongly reduced Id1 mRNA and protein expression...
  12. Takeda T, Sakata M, Isobe A, Yamamoto T, Nishimoto F, Minekawa R, et al. Involvement of Sp-1 in the regulation of the Id-1 gene during trophoblast cell differentiation. Placenta. 2007;28:192-8 pubmed
    ..The protein level of Sp-1 was decreased during the differentiation. These results suggest that the Sp-1 protein level may regulate the Box-2-binding activity and the trophoblast giant-cell differentiation. ..
  13. Belmokhtar K, Bourguignon T, Worou M, Khamis G, Bonnet P, Domenech J, et al. Regeneration of three layers vascular wall by using BMP2-treated MSC involving HIF-1? and Id1 expressions through JAK/STAT pathways. Stem Cell Rev. 2011;7:847-59 pubmed publisher
    ..We demonstrated using inhibitors and siRNAs that rh-BMP2 enhanced the expression of HIF-1? and Id1 through, at least in part, the stimulation of JAK2/STAT3/STAT5 signaling pathways...
  14. Tzeng S, Bresnahan J, Beattie M, de Vellis J. Upregulation of the HLH Id gene family in neural progenitors and glial cells of the rat spinal cord following contusion injury. J Neurosci Res. 2001;66:1161-72 pubmed
    ..Inhibitors of DNA binding (Id1, Id2, Id3) belong to a helix-loop-helix (HLH) gene family...
  15. Stewart H, Zoidl G, Rossner M, Brennan A, Zoidl C, Nave K, et al. Helix-loop-helix proteins in Schwann cells: a study of regulation and subcellular localization of Ids, REB, and E12/47 during embryonic and postnatal development. J Neurosci Res. 1997;50:684-701 pubmed
    ..In this study, we have analyzed the expression of the dominant negative HLH genes, Id1 to Id4 and the class A gene REB, during Schwann cell development...
  16. Kim A, Puram S, Bilimoria P, Ikeuchi Y, Keough S, Wong M, et al. A centrosomal Cdc20-APC pathway controls dendrite morphogenesis in postmitotic neurons. Cell. 2009;136:322-36 pubmed publisher
    ..The identification of a centrosomal Cdc20-APC ubiquitin signaling pathway holds important implications for diverse biological processes, including neuronal connectivity and plasticity. ..
  17. Sun X, Copeland N, Jenkins N, Baltimore D. Id proteins Id1 and Id2 selectively inhibit DNA binding by one class of helix-loop-helix proteins. Mol Cell Biol. 1991;11:5603-11 pubmed
    ..contains a helix-loop-helix motif similar to that of the previously described Id protein (referred to here as Id1), but the two proteins are different elsewhere...
  18. Kofler B, Bulleyment A, Humphries A, Carter D. Id-1 expression defines a subset of vimentin/S-100beta-positive, GFAP-negative astrocytes in the adult rat pineal gland. Histochem J. 2002;34:167-71 pubmed
    ..These results identify the rat pineal gland as a model system for the functional analysis of Id-1. ..
  19. Tzeng S, de Vellis J. Id1, Id2, and Id3 gene expression in neural cells during development. Glia. 1998;24:372-81 pubmed
    b>Id1, Id2, and Id3 mRNA are expressed mainly in the proliferating ependymal cell zone of the mouse brain during embryogenesis...
  20. Li W, Wang H, Kuang C, Zhu J, Yu Y, Qin Z, et al. An essential role for the Id1/PI3K/Akt/NFkB/survivin signalling pathway in promoting the proliferation of endothelial progenitor cells in vitro. Mol Cell Biochem. 2012;363:135-45 pubmed publisher
    ..The over-expression of the inhibitor of differentiation or DNA binding 1 (Id1) significantly improved EPC proliferation...
  21. Puram S, Kim A, Park H, Anckar J, Bonni A. The ubiquitin receptor S5a/Rpn10 links centrosomal proteasomes with dendrite development in the mammalian brain. Cell Rep. 2013;4:19-30 pubmed publisher
    ..We also find that the helix-loop-helix protein Id1 disrupts the interaction of S5a/Rpn10 with the proteasomal lid and thereby inhibits centrosomal proteasome ..
  22. Cao Y, Liu X, Zhang W, Deng X, Zhang H, Liu Y, et al. TGF-beta repression of Id2 induces apoptosis in gut epithelial cells. Oncogene. 2009;28:1089-98 pubmed publisher
    ..We found that TGF-beta repressed the expression of the inhibitor of differentiation (Id) gene family. Knockdown of Id1 and Id2 gene expression induced apoptosis in RIE-1 cells, whereas overexpression of Id2 attenuated TGF-beta-induced ..
  23. Liang D, Hu H, Li S, Dong J, Wang X, Wang Y, et al. Oncogenic herpesvirus KSHV Hijacks BMP-Smad1-Id signaling to promote tumorigenesis. PLoS Pathog. 2014;10:e1004253 pubmed publisher
    ..Importantly, our results demonstrate the efficacy of targeting the BMP-Smad1-Id pathway for inhibiting the growth of KSHV-induced tumors, and therefore identify the BMP pathway as a promising therapeutic target for KS...
  24. Springhorn J, Ellingsen O, Berger H, Kelly R, Smith T. Transcriptional regulation in cardiac muscle. Coordinate expression of Id with a neonatal phenotype during development and following a hypertrophic stimulus in adult rat ventricular myocytes in vitro. J Biol Chem. 1992;267:14360-5 pubmed
  25. Persson A, Bull C, Eriksson P. Requirement for Id1 in opioid-induced oligodendrogenesis in cultured adult rat hippocampal progenitors. Eur J Neurosci. 2006;23:2277-88 pubmed
    ..05), genes that are associated with oligodendrogenesis. Furthermore, beta-endorphin increased the levels of Id1, but not Id3, mRNA on the arrays...
  26. Fraidenraich D, Stillwell E, Romero E, Wilkes D, Manova K, Basson C, et al. Rescue of cardiac defects in id knockout embryos by injection of embryonic stem cells. Science. 2004;306:247-52 pubmed
    ..Thus, ES cells have the potential to reverse congenital defects through Id-dependent local and long-range effects in a mammalian embryo. ..
  27. Duffield G, Watson N, Mantani A, Peirson S, Robles Murguia M, Loros J, et al. A role for Id2 in regulating photic entrainment of the mammalian circadian system. Curr Biol. 2009;19:297-304 pubmed publisher
    Inhibitor of DNA binding genes (Id1-Id4) encode helix-loop-helix (HLH) transcriptional repressors associated with development and tumorigenesis [1, 2], but little is known concerning the function(s) of these genes in normal adult animals...
  28. Yu D, Hsieh D, Chang S. Increasing expression of GST-pi MIF, and ID1 genes in chemoresistant prostate cancer cells. Arch Androl. 2006;52:275-81 pubmed
    ..Overexpression of mRNAs in macrophage migration inhibitory factor (MIF), DNA binding protein inhibitor 1 (ID1), and glutathione S-transferase-pi (GST-pi) were confirmed by gene-specific RT-PCR...
  29. Einarson M, Chao M. Regulation of Id1 and its association with basic helix-loop-helix proteins during nerve growth factor-induced differentiation of PC12 cells. Mol Cell Biol. 1995;15:4175-83 pubmed
    ..Addition of exogeneous Id1 protein, a negative regulator of basic HLH proteins, disrupted specific complexes formed by PC12 cell nuclear ..
  30. Vincent K, Jones E, Arthur M, Smart D, Trim J, Wright M, et al. Regulation of E-box DNA binding during in vivo and in vitro activation of rat and human hepatic stellate cells. Gut. 2001;49:713-9 pubmed
    ..Western and northern blot analysis of MyoD and Id1 basic helix-loop-helix (bHLH) proteins was performed to confirm expression in HSC...
  31. Wen D, Zou Y, Gao Y, Zhao Q, Xie Y, Shen P, et al. Inhibitor of DNA Binding 1 Is Induced during Kidney Ischemia-Reperfusion and Is Critical for the Induction of Hypoxia-Inducible Factor-1?. Biomed Res Int. 2016;2016:4634386 pubmed publisher
    ..models of hypoxia-reoxygenation (H/R) were established to investigate the expression of inhibitor of DNA binding 1 (ID1) in AKI, and the regulation relationship between ID1 and hypoxia-inducible factor 1 alpha (HIF-1?)...
  32. Hung Y, Chang S, Huang C, Yin J, Hwang C, Yang L, et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol Neurobiol. 2016;53:793-809 pubmed publisher
    ..Aβ25-35 also time-dependently induced inhibitor of differentiation-1 (Id1) that has been shown to stabilize HIF-1α; further, Aβ25-35-mediated induction of HIF-1α and SHH was both ..
  33. Tanaka A, Itoh F, Nishiyama K, Takezawa T, Kurihara H, Itoh S, et al. Inhibition of endothelial cell activation by bHLH protein E2-2 and its impairment of angiogenesis. Blood. 2010;115:4138-47 pubmed publisher
    ..In this paper, we demonstrate that E2-2 interacts with Id1 and provide evidence that this interaction potentiates angiogenesis...
  34. Zhang H, Lawson W, Polosukhin V, Pozzi A, Blackwell T, Litingtung Y, et al. Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice. Am J Pathol. 2007;171:1113-26 pubmed
    ..However, the specific role of Id1 in lung injury has not been investigated...
  35. Ozeki M, Hamajima Y, Feng L, Ondrey F, Schlentz E, Lin J. Id1 induces the proliferation of cochlear sensory epithelial cells via the nuclear factor-kappaB/cyclin D1 pathway in vitro. J Neurosci Res. 2007;85:515-24 pubmed
    ..In this study, we demonstrate the Id1 gene was expressed in the rapidly growing otocyst on embryonic day 12 (E12) and in the organ of Corti, spiral ..