Gene Symbol: Grin3a
Description: glutamate ionotropic receptor NMDA type subunit 3A
Alias: GluN3A, NR3, chi-1, glutamate receptor ionotropic, NMDA 3A, N-methyl-D-aspartate receptor subtype 3A, NMDAR-L, NMDAR-L1, NMDAR3A, NR3 chi-1, NR3A, glutamate [NMDA] receptor subunit 3A, glutamate receptor chi-1, glutamate receptor, ionotropic, N-methyl-D-aspartate 3A
Species: rat
Products:     Grin3a

Top Publications

  1. Ciabarra A, Sullivan J, Gahn L, Pecht G, Heinemann S, Sevarino K. Cloning and characterization of chi-1: a developmentally regulated member of a novel class of the ionotropic glutamate receptor family. J Neurosci. 1995;15:6498-508 pubmed
    ..Further characterization will be required to establish the precise role of this glutamate receptor subunit in neuronal signaling. ..
  2. Ma O, Sucher N. Molecular interaction of NMDA receptor subunit NR3A with protein phosphatase 2A. Neuroreport. 2004;15:1447-50 pubmed
    ..protein phosphatase 2A (PP2A) was previously found to bind to the carboxyl domain of NMDA receptor (NMDAR) subunit NR3A. We now report that NR3A constitutively associates with the PP2A holoenzyme, but not the core enzyme in rat brain ..
  3. Perez Otano I, Schulteis C, Contractor A, Lipton S, Trimmer J, Sucher N, et al. Assembly with the NR1 subunit is required for surface expression of NR3A-containing NMDA receptors. J Neurosci. 2001;21:1228-37 pubmed
    ..Coexpression of NR3A, an additional subunit of the NMDA receptor family, modifies NMDA-mediated responses...
  4. Sasaki Y, Rothe T, Premkumar L, Das S, Cui J, Talantova M, et al. Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons. J Neurophysiol. 2002;87:2052-63 pubmed
    Recently, we cloned and began to characterize a new N-methyl-D-aspartate receptor (NMDAR) subunit, NR3A. Here we extend our earlier findings by showing that recombinantly expressed NR3A in COS cells is biochemically associated with both ..
  5. Bendova Z, Sumova A, Mikkelsen J. Circadian and developmental regulation of N-methyl-d-aspartate-receptor 1 mRNA splice variants and N-methyl-d-aspartate-receptor 3 subunit expression within the rat suprachiasmatic nucleus. Neuroscience. 2009;159:599-609 pubmed publisher
    ..During ontogenesis, expression of two of the NMDAR1 subunit splice variants, as well as the NMDAR3A and 3B subunits, exhibits developmental loss around the time of eye opening...
  6. Brody S, Nakanishi N, Tu S, Lipton S, Geyer M. A developmental influence of the N-methyl-D-aspartate receptor NR3A subunit on prepulse inhibition of startle. Biol Psychiatry. 2005;57:1147-52 pubmed
    ..NMDA) receptor is composed of various conformations of multiple subunits (including NR1, NR2A-D, and NR3A-B). Peak expression of the NR3A subunit occurs approximately 2-3 weeks postnatal, with low levels in adulthood...
  7. Wada A, Takahashi H, Lipton S, Chen H. NR3A modulates the outer vestibule of the "NMDA" receptor channel. J Neurosci. 2006;26:13156-66 pubmed
    ..system and the SCAM (substituted cysteine accessibility method), we found that M3 segments of both NR1 and NR3A form a narrow constriction in the outer vestibule of the channel, which prevents passage of externally applied ..
  8. Sucher N, Akbarian S, Chi C, Leclerc C, Awobuluyi M, Deitcher D, et al. Developmental and regional expression pattern of a novel NMDA receptor-like subunit (NMDAR-L) in the rodent brain. J Neurosci. 1995;15:6509-20 pubmed
    ..While the function of NMDAR-L remains to be established, its developmental and regional expression pattern suggests that NMDAR-L may influence axonal outgrowth and synaptogenesis during brain development. ..
  9. Das S, Sasaki Y, Rothe T, Premkumar L, Takasu M, Crandall J, et al. Increased NMDA current and spine density in mice lacking the NMDA receptor subunit NR3A. Nature. 1998;393:377-81 pubmed
    ..Here we report the characterization of a regulatory NMDAR subunit, NR3A (formerly termed NMDAR-L or chi-1), which is expressed primarily during brain development...

More Information


  1. Fukumori R, Takarada T, Nakamichi N, Kambe Y, Kawagoe H, Nakazato R, et al. Requirement of both NR3A and NR3B subunits for dominant negative properties on Ca2+ mobilization mediated by acquired N-methyl-D-aspartate receptor channels into mitochondria. Neurochem Int. 2010;57:730-7 pubmed publisher
    ..Although recent studies identified dominant negative NR3A and NR3B subunits, whether these subunits inhibit Ca(2+) mobilization through NMDAR channels into mitochondria is ..
  2. Nilsson A, Duan J, Mo Boquist L, Benedikz E, Sundstrom E. Characterisation of the human NMDA receptor subunit NR3A glycine binding site. Neuropharmacology. 2007;52:1151-9 pubmed
    In this study, we characterise the binding site of the human N-methyl-d-aspartate (NMDA) receptor subunit NR3A. Saturation radioligand binding of the NMDA receptor agonists [(3)H]-glycine and [(3)H]-glutamate showed that only glycine ..
  3. Vilches N, Spichiger C, Méndez N, Abarzua Catalan L, Galdames H, Hazlerigg D, et al. Gestational chronodisruption impairs hippocampal expression of NMDA receptor subunits Grin1b/Grin3a and spatial memory in the adult offspring. PLoS ONE. 2014;9:e91313 pubmed publisher
    ..The present study also supports that developmental plasticity in response to photoperiodic cues may be modulated by maternal melatonin. ..
  4. Sucher N, Kohler K, Tenneti L, Wong H, Gründer T, Fauser S, et al. N-methyl-D-aspartate receptor subunit NR3A in the retina: developmental expression, cellular localization, and functional aspects. Invest Ophthalmol Vis Sci. 2003;44:4451-6 pubmed
    Recently, a novel N-methyl-D-aspartate receptor (NMDAR) subunit, NR3A, has been discovered in the brain and shown to decrease NMDAR activity by modulating the calcium permeability of the receptor channel...
  5. Nilsson A, Eriksson M, Muly E, Akesson E, Samuelsson E, Bogdanovic N, et al. Analysis of NR3A receptor subunits in human native NMDA receptors. Brain Res. 2007;1186:102-12 pubmed
    b>NR3A, representing the third class of NMDA receptor subunits, was first studied in rats, demonstrating ubiquitous expression in the developing central nervous system (CNS), but in the adult mainly expressed in spinal cord and some ..
  6. Smothers C, Woodward J. Pharmacological characterization of glycine-activated currents in HEK 293 cells expressing N-methyl-D-aspartate NR1 and NR3 subunits. J Pharmacol Exp Ther. 2007;322:739-48 pubmed
    ..we used voltage-clamp electrophysiology to characterize excitatory glycine-activated receptors assembled from NR1, NR3A, and NR3B subunits expressed in human embryonic kidney (HEK) 293 cells...
  7. Henson M, Larsen R, Lawson S, Pérez Otaño I, Nakanishi N, Lipton S, et al. Genetic deletion of NR3A accelerates glutamatergic synapse maturation. PLoS ONE. 2012;7:e42327 pubmed publisher critically dependent upon activation of NMDA-type glutamate receptors (NMDARs); however, the contributions of NR3A subunit-containing NMDARs to this process have only begun to be considered...
  8. Smothers C, Woodward J. Effect of the NR3 subunit on ethanol inhibition of recombinant NMDA receptors. Brain Res. 2003;987:117-21 pubmed
    ..Overall, these results suggest that the NR3A subunit is not a determinant of ethanol sensitivity in recombinant NMDA receptors.
  9. Sun L, Margolis F, Shipley M, Lidow M. Identification of a long variant of mRNA encoding the NR3 subunit of the NMDA receptor: its regional distribution and developmental expression in the rat brain. FEBS Lett. 1998;441:392-6 pubmed
    A longer variant of rat mRNA encoding the NR3 subunit of the NMDA receptor has been identified. It contains a 60-bp insertion at the nucleotide position 3007 in the intracellular domain of the C-terminal of the previously cloned variant...
  10. Eriksson M, Samuelsson H, Samuelsson E, Liu L, McKeehan W, Benedikz E, et al. The NMDAR subunit NR3A interacts with microtubule-associated protein 1S in the brain. Biochem Biophys Res Commun. 2007;361:127-32 pubmed
    When screening a brain cDNA library, we found that the N-methyl-D-aspartate receptor subunit NR3A binds to microtubule-associated protein (MAP) 1S/chromosome 19 open reading frame 5 (C19ORF5)...
  11. Jin C, Smothers C, Woodward J. Enhanced ethanol inhibition of recombinant N-methyl-D-aspartate receptors by magnesium: role of NR3A subunits. Alcohol Clin Exp Res. 2008;32:1059-66 pubmed publisher
    ..sensitivity of recombinant NMDA receptors and how ethanol inhibition under these conditions is influenced by the NR3A subunit. Recombinant cDNAs encoding NMDA receptor subunits were expressed in human embryonic kidney 293 cells...
  12. Sanhueza M, Fernandez Villalobos G, Stein I, Kasumova G, Zhang P, Bayer K, et al. Role of the CaMKII/NMDA receptor complex in the maintenance of synaptic strength. J Neurosci. 2011;31:9170-8 pubmed publisher
    ..These results support the hypothesis that the CaMKII/NMDAR complex has switch-like properties that are important in the maintenance of synaptic strength. ..
  13. Yao Y, Belcher J, Berger A, Mayer M, Lau A. Conformational analysis of NMDA receptor GluN1, GluN2, and GluN3 ligand-binding domains reveals subtype-specific characteristics. Structure. 2013;21:1788-99 pubmed publisher
    ..GluN1 and GluN3 bind glycine, whereas GluN2 binds glutamate. Crystal structures of the GluN1 and GluN3A ligand-binding domains (LBDs) in their apo states unexpectedly reveal open- and closed-cleft conformations, ..
  14. Takarada T, Takahata Y, Iemata M, Hinoi E, Uno K, Hirai T, et al. Interference with cellular differentiation by D-serine through antagonism at N-methyl-D-aspartate receptors composed of NR1 and NR3A subunits in chondrocytes. J Cell Physiol. 2009;220:756-64 pubmed publisher the introduction of runt-related transcription factor-2 (Runx2) in COS7 cells transfected with NR1 and NR3A subunits, while D-Ser significantly prevented the increase by Gly without affecting the promoter activity of Runx2...
  15. Zhang X, Ji F, Wang N, Chen L, Tian T, Lu W. Glycine induces bidirectional modifications in N-methyl-D-aspartate receptor-mediated synaptic responses in hippocampal CA1 neurons. J Biol Chem. 2014;289:31200-11 pubmed publisher
    ..Our present findings reveal important functions of the two glycine binding sites in gating the direction of synaptic plasticity in NMDA responses. ..
  16. Ishihama K, Kogo M, Wakisaka S, Turman J. Prenatal development of NMDA receptor composition and function in trigeminal neurons. Arch Histol Cytol. 2005;68:321-35 pubmed
    ..NMDA receptor immunohistochemistry studies, using antibodies directed against subunits NR1, NR2A, NR2B, NR3A and NR3B were performed to determine the prenatal regulation of NMDA subunits in trigeminal motoneurons (Mo5), and ..
  17. Wang P, Petralia R, Wang Y, Wenthold R, Brenowitz S. Functional NMDA receptors at axonal growth cones of young hippocampal neurons. J Neurosci. 2011;31:9289-97 pubmed publisher
    ..Our findings reveal the presence of functional NMDARs in axons and growth cones of young neurons, suggesting a role for these receptors in axonal guidance and synapse formation during neuronal development. ..
  18. Chan S, Sucher N. An NMDA receptor signaling complex with protein phosphatase 2A. J Neurosci. 2001;21:7985-92 pubmed
    ..The NMDAR subunit NR3A is equipped with a unique carboxyl domain that is different from other NMDAR subunits...
  19. Qu Z, Miao W, Hu S, Li C, Zhuo X, Zong Y, et al. N-methyl-D-aspartate receptor-dependent denitrosylation of neuronal nitric oxide synthase increase the enzyme activity. PLoS ONE. 2012;7:e52788 pubmed publisher
    ..nNOS dephosphorylation may be induced by the enzyme denitrosylation, which suggest that S-nitrosylation/denitrosylation of nNOS may be an important mechanism in regulating the enzyme activity. ..
  20. Al Hallaq R, Jarabek B, Fu Z, Vicini S, Wolfe B, Yasuda R. Association of NR3A with the N-methyl-D-aspartate receptor NR1 and NR2 subunits. Mol Pharmacol. 2002;62:1119-27 pubmed
    The NR3A subunit of the N-methyl-D-aspartate receptor has been shown to form glutamatergic receptor complexes with NR1 and NR2 subunits and excitatory glycinergic receptor complexes with NR1 alone...
  21. Wang H, Yan H, Zhang S, Wei X, Zheng J, Li J. The GluN3A subunit exerts a neuroprotective effect in brain ischemia and the hypoxia process. ASN Neuro. 2013;5:231-42 pubmed publisher
    ..GluN3 subunits, the third member of the NMDAR family with two isoforms, GluN3A and GluN3B, have been confirmed to display an inhibitory effect on NMDAR activity...
  22. Fiuza M, González González I, Pérez Otaño I. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling. Proc Natl Acad Sci U S A. 2013;110:20807-12 pubmed publisher
    ..GluN2 subunits mediate synapse plasticity and promote spine growth and stabilization, juvenile NMDARs containing GluN3A subunits are thought to inhibit these processes via yet unknown mechanisms...