Gene Symbol: Gpsm1
Description: G-protein signaling modulator 1
Alias: Ags3, G-protein-signaling modulator 1, G-protein signaling modulator 1 (AGS3-like, C. elegans), G-protein signalling modulator 1 (AGS3-like, C. elegans), activator of G-protein signaling 3
Species: rat
Products:     Gpsm1

Top Publications

  1. Ma H, Peterson Y, Bernard M, Lanier S, Graber S. Influence of cytosolic AGS3 on receptor--G protein coupling. Biochemistry. 2003;42:8085-93 pubmed
    b>Activator of G protein signaling 3 (AGS3) activates the Gbetagamma mating pathway in yeast in a manner that is independent of heptahelical receptors...
  2. Takesono A, Cismowski M, Ribas C, Bernard M, Chung P, Hazard S, et al. Receptor-independent activators of heterotrimeric G-protein signaling pathways. J Biol Chem. 1999;274:33202-5 pubmed
    ..We identified two mammalian proteins, AGS2 and AGS3 (activators of G-protein signaling), that activated the pheromone response pathway at the level of heterotrimeric G-..
  3. Pattingre S, De Vries L, Bauvy C, Chantret I, Cluzeaud F, Ogier Denis E, et al. The G-protein regulator AGS3 controls an early event during macroautophagy in human intestinal HT-29 cells. J Biol Chem. 2003;278:20995-1002 pubmed
    b>AGS3 contains GoLoco or G-protein regulatory motifs in its COOH-terminal half that stabilize the GDP-bound conformation of the alpha-subunit of the trimeric Gi3 protein...
  4. Blumer J, Chandler L, Lanier S. Expression analysis and subcellular distribution of the two G-protein regulators AGS3 and LGN indicate distinct functionality. Localization of LGN to the midbody during cytokinesis. J Biol Chem. 2002;277:15897-903 pubmed
    Activator of G-protein signaling 3 (AGS3) and LGN have a similar domain structure and contain four G-protein regulatory motifs that serve as anchors for the binding of the GDP-bound conformation of specific G-protein alpha subunits...
  5. Peterson Y, Bernard M, Ma H, Hazard S, Graber S, Lanier S. Stabilization of the GDP-bound conformation of Gialpha by a peptide derived from the G-protein regulatory motif of AGS3. J Biol Chem. 2000;275:33193-6 pubmed
    The G-protein regulatory (GPR) motif in AGS3 was recently identified as a region for protein binding to heterotrimeric G-protein alpha subunits...
  6. An N, Blumer J, Bernard M, Lanier S. The PDZ and band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J Biol Chem. 2008;283:24718-28 pubmed publisher
    Activator of G-protein signaling 3 (AGS3) is one of nine mammalian proteins containing one or more G-protein regulatory (GPR) motifs that stabilize the GDP-bound conformation of Galphai...
  7. Yao L, McFarland K, Fan P, Jiang Z, Inoue Y, Diamond I. Activator of G protein signaling 3 regulates opiate activation of protein kinase A signaling and relapse of heroin-seeking behavior. Proc Natl Acad Sci U S A. 2005;102:8746-51 pubmed
    ..Activation requires Galpha(i3) and an activator of G protein signaling 3 (AGS3)...
  8. Bowers M, McFarland K, Lake R, Peterson Y, Lapish C, Gregory M, et al. Activator of G protein signaling 3: a gatekeeper of cocaine sensitization and drug seeking. Neuron. 2004;42:269-81 pubmed
    Chronic cocaine administration reduces G protein signaling efficacy. Here, we report that the expression of AGS3, which binds to GialphaGDP and inhibits GDP dissociation, was upregulated in the prefrontal cortex (PFC) during late ..
  9. de Vries L, Fischer T, Tronchere H, Brothers G, Strockbine B, Siderovski D, et al. Activator of G protein signaling 3 is a guanine dissociation inhibitor for Galpha i subunits. Proc Natl Acad Sci U S A. 2000;97:14364-9 pubmed
    b>Activator of G protein signaling 3 (AGS3) is a newly identified protein shown to act at the level of the G protein itself. AGS3 belongs to the GoLoco family of proteins, sharing the 19-aa GoLoco motif that is a Galpha(i/o) binding motif...

More Information


  1. Bowers M, Hopf F, Chou J, Guillory A, Chang S, Janak P, et al. Nucleus accumbens AGS3 expression drives ethanol seeking through G betagamma. Proc Natl Acad Sci U S A. 2008;105:12533-8 pubmed publisher
    ..A novel G protein modulator (Gpsm1/AGS3) was up-regulated in the rat nucleus accumbens core (NAcore) but not in other limbic nuclei during abstinence ..
  2. Natochin M, Lester B, Peterson Y, Bernard M, Lanier S, Artemyev N. AGS3 inhibits GDP dissociation from galpha subunits of the Gi family and rhodopsin-dependent activation of transducin. J Biol Chem. 2000;275:40981-5 pubmed
    ..b>Activator of G protein signaling 3 (AGS3), identified as an activator of the yeast pheromone pathway in the absence of the pheromone ..
  3. Bernard M, Peterson Y, Chung P, Jourdan J, Lanier S. Selective interaction of AGS3 with G-proteins and the influence of AGS3 on the activation state of G-proteins. J Biol Chem. 2001;276:1585-93 pubmed
    b>AGS3 (activator of G-protein signaling 3) was isolated in a yeast-based functional screen for receptor-independent activators of heterotrimeric G-proteins...
  4. Sanada K, Tsai L. G protein betagamma subunits and AGS3 control spindle orientation and asymmetric cell fate of cerebral cortical progenitors. Cell. 2005;122:119-31 pubmed
    ..Silencing AGS3, a nonreceptor activator of Gbetagamma, results in defects similar to the impairment of Gbetagamma, providing ..
  5. Izaki T, Kamakura S, Kohjima M, Sumimoto H. Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3. Biochem Biophys Res Commun. 2006;341:1001-6 pubmed
    ..Nevertheless, human Insc proteins bind to the human Pins homologues LGN and AGS3, and also to human Par3 and its related protein Par3beta...
  6. Conley J, Watts V. Differential effects of AGS3 expression on D(2L) dopamine receptor-mediated adenylyl cyclase signaling. Cell Mol Neurobiol. 2013;33:551-8 pubmed publisher
    b>Activator of G protein signaling 3 (AGS3) binds G?(i) subunits in the GDP-bound state, implicating AGS3 as an important regulator of G?(i)-linked receptor (e.g., D2 dopamine and ?-opioid) signaling...
  7. Wang W, Li Q, Zou F, Yu Z, Wang Y, Lu T, et al. Increased expression of AGS3 in rat brain cortex after traumatic brain injury. J Neurosci Res. 2013;91:726-36 pubmed publisher
    ..Previous studies have demonstrated that AGS3, which belongs to the AGS family, is involved in a number of different cellular activities...
  8. Yuzawa S, Kamakura S, Iwakiri Y, Hayase J, Sumimoto H. Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN). Proc Natl Acad Sci U S A. 2011;108:19210-5 pubmed publisher
    ..residues that are critical for mInsc-LGN association, and reveal that the activator of G-protein signaling 3 (AGS3)-binding protein Frmpd1 [4...
  9. Vural A, Oner S, An N, Simon V, Ma D, Blumer J, et al. Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable. Mol Cell Biol. 2010;30:1528-40 pubmed publisher
    b>AGS3, a receptor-independent activator of G-protein signaling, is involved in unexpected functional diversity for G-protein signaling systems...
  10. Thomas C, Tall G, Adhikari A, Sprang S. Ric-8A catalyzes guanine nucleotide exchange on G alphai1 bound to the GPR/GoLoco exchange inhibitor AGS3. J Biol Chem. 2008;283:23150-60 pubmed publisher
    ..A host of proteins, including Galpha subunits, Ric-8, AGS3, regulators of G-protein signalings, and scaffolding proteins, coordinate this vital cellular process...
  11. Adhikari A, Sprang S. Thermodynamic characterization of the binding of activator of G protein signaling 3 (AGS3) and peptides derived from AGS3 with G alpha i1. J Biol Chem. 2003;278:51825-32 pubmed
    b>Activator of G protein signaling 3 (AGS3) is a guanine nucleotide dissociation inhibitor (GDI) that contains four G protein regulatory (GPR) or GoLoco motifs in its C-terminal domain...
  12. Ghosh M, Peterson Y, Lanier S, Smrcka A. Receptor- and nucleotide exchange-independent mechanisms for promoting G protein subunit dissociation. J Biol Chem. 2003;278:34747-50 pubmed
    ..b>AGS3, a member of the activators of G protein signaling family of proteins, also activates G protein signaling in a ..
  13. Sato M, Gettys T, Lanier S. AGS3 and signal integration by Galpha(s)- and Galpha(i)-coupled receptors: AGS3 blocks the sensitization of adenylyl cyclase following prolonged stimulation of a Galpha(i)-coupled receptor by influencing processing of Galpha(i). J Biol Chem. 2004;279:13375-82 pubmed
    b>AGS3-LONG and AGS3-SHORT contain G-protein regulatory motifs that interact with and stabilize the GDP-bound conformation of Galpha(i) > Galpha(o)...
  14. Xu Z, Xia B, Gong Q, Bailey J, Groves B, Radeke M, et al. Identification of a deubiquitinating enzyme as a novel AGS3-interacting protein. PLoS ONE. 2010;5:e9725 pubmed publisher
    b>Activator of G protein Signaling 3 (AGS3) is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/..
  15. Regner K, Nozu K, Lanier S, Blumer J, Avner E, Sweeney W, et al. Loss of activator of G-protein signaling 3 impairs renal tubular regeneration following acute kidney injury in rodents. FASEB J. 2011;25:1844-55 pubmed publisher
    ..In this report, we demonstrate that activator of G-protein signaling 3 (AGS3), an unconventional receptor-independent regulator of heterotrimeric G-protein function, influences renal tubular ..
  16. Blumer J, Bernard M, Peterson Y, Nezu J, Chung P, Dunican D, et al. Interaction of activator of G-protein signaling 3 (AGS3) with LKB1, a serine/threonine kinase involved in cell polarity and cell cycle progression: phosphorylation of the G-protein regulatory (GPR) motif as a regulatory mechanism for the interaction . J Biol Chem. 2003;278:23217-20 pubmed
    Activator of G-protein signaling 3 (AGS3) has a modular domain structure consisting of seven tetratricopeptide repeats (TPRs) and four G-protein regulatory (GPR) motifs...
  17. Pizzinat N, Takesono A, Lanier S. Identification of a truncated form of the G-protein regulator AGS3 in heart that lacks the tetratricopeptide repeat domains. J Biol Chem. 2001;276:16601-10 pubmed
    b>AGS3, a 650-amino acid protein encoded by an approximately 4-kilobase (kb) mRNA enriched in rat brain, is a Galpha(i)/Galpha(t)-binding protein that competes with Gbetagamma for interaction with Galpha(GDP) and acts as a guanine ..
  18. Taymans J, Kia H, Langlois X. Activator of G protein signaling type 3 mRNA is widely distributed in the rat brain and is particularly abundant in the subventricular zone-olfactory bulb system of neural precursor cell proliferation, migration and differentiation. Neurosci Lett. 2006;391:116-21 pubmed
    b>Activator of G protein signaling 3 (AGS3) is a guanine nucleotide dissociation inhibitor (GDI) to heterotrimeric G proteins of the Galphai/o class...