Gnpat

Summary

Gene Symbol: Gnpat
Description: glyceronephosphate O-acyltransferase
Alias: dihydroxyacetone phosphate acyltransferase, DAP-AT, DHAP-AT, acyl-CoA:dihydroxyacetonephosphate acyltransferase, acyl-CoA:dihydroxyacetonephosphateacyltransferase, glycerone-phosphate O-acyltransferase
Species: rat
Products:     Gnpat

Top Publications

  1. Ruyter B, Lund J, Thomassen M, Christiansen E. Studies of dihydroxyacetone phosphate acyltransferase in rat small intestine. Subcellular localization and effect of partially hydrogenated fish oil and clofibrate. Biochem J. 1992;282 ( Pt 2):565-70 pubmed
    The subcellular localization of dihydroxyacetone phosphate acyltransferase (DHAPAT) activity in rat small intestine was investigated by Nycodenz-gradient centrifugation...
  2. Thai T, Rodemer C, Jauch A, Hunziker A, Moser A, Gorgas K, et al. Impaired membrane traffic in defective ether lipid biosynthesis. Hum Mol Genet. 2001;10:127-36 pubmed
    ..The data suggest that plasmalogens, analogous to cholesterol, are essential for correct membrane functioning and their deficiency results in impaired membrane trafficking...
  3. Rodemer C, Thai T, Brugger B, Kaercher T, Werner H, Nave K, et al. Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice. Hum Mol Genet. 2003;12:1881-95 pubmed
  4. Liu D, Nagan N, Just W, Rodemer C, Thai T, Zoeller R. Role of dihydroxyacetonephosphate acyltransferase in the biosynthesis of plasmalogens and nonether glycerolipids. J Lipid Res. 2005;46:727-35 pubmed
    ..These data demonstrate that a defect in the gene that codes for peroxisomal DHAPAT is the primary lesion in the NRel-4 cell line and that the peroxisomal DHAPAT is essential for the biosynthesis of plasmalogens in animal cells. ..
  5. Teigler A, Komljenovic D, Draguhn A, Gorgas K, Just W. Defects in myelination, paranode organization and Purkinje cell innervation in the ether lipid-deficient mouse cerebellum. Hum Mol Genet. 2009;18:1897-908 pubmed publisher
    ..Common molecular mechanisms that may underlie these phenotypes are discussed...
  6. Bates E, Saggerson E. A study of the glycerol phosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activities in rat liver mitochondrial and microsomal fractions. Relative distribution in parenchymal and non-parenchymal cells, effects of N-ethylmaleimi. Biochem J. 1979;182:751-62 pubmed
    1. GPAT (glycerol phosphate acyltransferase) and DHAPAT (dihydroxyacetone phosphate acyltransferase) activities were measured both in subcellular fractions prepared from fed rat liver and in whole homogenates prepared from freeze-stopped ..
  7. Ofman R, Hettema E, Hogenhout E, Caruso U, Muijsers A, Wanders R. Acyl-CoA:dihydroxyacetonephosphate acyltransferase: cloning of the human cDNA and resolution of the molecular basis in rhizomelic chondrodysplasia punctata type 2. Hum Mol Genet. 1998;7:847-53 pubmed
    ..All RCDP type 2 patients analyzed were found to contain mutations in their DHAPAT cDNA. This demonstrates that RCDP type 2 is the result of mutations in DHAPAT...
  8. Skorve J, Ruyter B, Rustan A, Christiansen E, Drevon C, Berge R. Effect of 3- and 4-thia-substituted fatty acids on glycerolipid metabolism and mitochondrial beta-oxidation in rat liver. Biochem Pharmacol. 1990;40:2005-12 pubmed
    ..by alkylthiopropionic acid (CETTD), resulted in a dose- and time-dependent increase in total dihydroxyacetone phosphate acyltransferase (DHAPAT) activity, in extent comparable to that of 3-thiadicarboxylic acid (BCMTD) and ..
  9. Ofman R, Wanders R. Purification of peroxisomal acyl-CoA: dihydroxyacetonephosphate acyltransferase from human placenta. Biochim Biophys Acta. 1994;1206:27-34 pubmed
    ..A value of about 10% was found, which closely corresponds to the residual amount of enzyme activity. ..

More Information

Publications13

  1. Thai T, Heid H, Rackwitz H, Hunziker A, Gorgas K, Just W. Ether lipid biosynthesis: isolation and molecular characterization of human dihydroxyacetonephosphate acyltransferase. FEBS Lett. 1997;420:205-11 pubmed
    ..Monospecific antibodies raised against this polypeptide efficiently immunoprecipitated DAP-AT activity from solubilized peroxisomal preparations, thus demonstrating that the cloned cDNA codes for DAP-AT. ..
  2. Reisse S, Rothardt G, Volkl A, Beier K. Peroxisomes and ether lipid biosynthesis in rat testis and epididymis. Biol Reprod. 2001;64:1689-94 pubmed
    ..The results suggest that peroxisomes in epithelial cells of the rat epididymis play a pivotal role in the biosynthesis of plasmalogens destined for delivery to the sperm plasma membrane. ..
  3. Hogenboom S, Romeijn G, Houten S, Baes M, Wanders R, Waterham H. Absence of functional peroxisomes does not lead to deficiency of enzymes involved in cholesterol biosynthesis. J Lipid Res. 2002;43:90-8 pubmed
    ..Our data provide an explanation for the conflicting findings in the literature and show that great care should be taken in the interpretation of data obtained in postmortem material. ..
  4. Thai T, Rodemer C, Worsch J, Hunziker A, Gorgas K, Just W. Synthesis of plasmalogens in eye lens epithelial cells. FEBS Lett. 1999;456:263-8 pubmed