Gene Symbol: Gnmt
Description: glycine N-methyltransferase
Alias: glycine N-methyltransferase, folate-binding protein, glycine methyltransferase
Species: rat
Products:     Gnmt

Top Publications

  1. Ogawa H, Konishi K, Takata Y, Nakashima H, Fujioka M. Rat glycine methyltransferase. Complete amino acid sequence deduced from a cDNA clone and characterization of the genomic DNA. Eur J Biochem. 1987;168:141-51 pubmed
    The amino terminus of glycine methyltransferase from rat liver is blocked...
  2. Lu S, Mato J. S-adenosylmethionine in liver health, injury, and cancer. Physiol Rev. 2012;92:1515-42 pubmed publisher
    ..This can result from inactive mutation of the enzyme glycine N-methyltransferase (GNMT)...
  3. Luka Z, Pakhomova S, Loukachevitch L, Newcomer M, Wagner C. Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate. Biochim Biophys Acta. 2012;1824:286-91 pubmed publisher
    Glycine N-methyltransferase (GNMT) is a key regulatory enzyme in methyl group metabolism...
  4. McMullen M, Rowling M, Ozias M, Schalinske K. Activation and induction of glycine N-methyltransferase by retinoids are tissue- and gender-specific. Arch Biochem Biophys. 2002;401:73-80 pubmed
    Glycine N-methyltransferase (GNMT) is a key protein in the liver that functions to regulate S-adenosylmethionine (SAM) and the SAM/S-adenosylhomocysteine ratio. Significant GNMT expression is also present in the kidney and pancreas...
  5. Rowling M, Schalinske K. Retinoic acid and glucocorticoid treatment induce hepatic glycine N-methyltransferase and lower plasma homocysteine concentrations in rats and rat hepatoma cells. J Nutr. 2003;133:3392-8 pubmed
    ..Glycine N-methyltransferase (GNMT) is a key protein that functions to regulate the supply and utilization of methyl groups for S-adenosylmethionine (..
  6. Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer M. Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes. Proteins. 2004;57:331-7 pubmed
    ..The crystal structure for the rat enzyme was published previously. Human and mouse GNMT were expressed in Escherichia coli in order to determine their crystal structures...
  7. Luka Z, Loukachevitch L, Wagner C. Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate. Biochim Biophys Acta. 2008;1784:1342-6 pubmed publisher
    Native liver glycine N-methyltransferase (GNMT) is N-acetylated while the recombinant enzyme is not. We show here that acetylation of the N-terminal valine affects several kinetic parameters of the enzyme...
  8. Liu S, Li Y, Chen Y, Chiang E, Li A, Lee Y, et al. Glycine N-methyltransferase-/- mice develop chronic hepatitis and glycogen storage disease in the liver. Hepatology. 2007;46:1413-25 pubmed
    Glycine N-methyltransferase (GNMT) affects genetic stability by regulating DNA methylation and interacting with environmental carcinogens...
  9. Yeo E, Wagner C. Tissue distribution of glycine N-methyltransferase, a major folate-binding protein of liver. Proc Natl Acad Sci U S A. 1994;91:210-4 pubmed
    Glycine N-methyltransferase (GNMT; S-adenosyl-L-methionine:glycine N-methyltransferase, EC is a major protein in rat liver that binds 5-methyltetrahydrofolate polyglutamate in vivo...

More Information


  1. Cavuoto P, Fenech M. A review of methionine dependency and the role of methionine restriction in cancer growth control and life-span extension. Cancer Treat Rev. 2012;38:726-36 pubmed publisher
    ..The application of nutritional methionine restriction and methioninase in combination with chemotherapeutic regimens is the current focus of clinical studies. ..
  2. Sterling K. 4S polycyclic aromatic hydrocarbon receptor (glycine N-methyltransferase) and the aryl hydrocarbon receptor nuclear translocator (hypoxia inducible factor-1?) interaction in Chinese hamster ovary and rat hepatoma cells: 4S PAH-R/ARNT hetero-oligomers. J Cell Biochem. 2011;112:2015-8 pubmed publisher
    Rat CYP1A1 promoter-luciferase, transiently transfected wild-type and 4S PAH receptor (glycine N-methyl transferase, GNMT)-transformed Chinese hamster ovary (CHO) cells were exposed to benzo[a]pyrene and assayed for luciferase activity as ..
  3. Kharbanda K, Vigneswara V, McVicker B, Newlaczyl A, Bailey K, Tuma D, et al. Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats. Biochem Biophys Res Commun. 2009;381:523-7 pubmed publisher
    ..Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress. ..
  4. Nieman K, Rowling M, Garrow T, Schalinske K. Modulation of methyl group metabolism by streptozotocin-induced diabetes and all-trans-retinoic acid. J Biol Chem. 2004;279:45708-12 pubmed
    The hepatic enzyme glycine N-methyltransferase (GNMT) plays a major role in the control of methyl group and homocysteine metabolism...
  5. Rowling M, McMullen M, Schalinske K. Vitamin A and its derivatives induce hepatic glycine N-methyltransferase and hypomethylation of DNA in rats. J Nutr. 2002;132:365-9 pubmed
    ..SAM) and the SAM/S-adenosylhomocysteine (SAH) ratio by the key cytosolic enzyme glycine N-methyltransferase (GNMT) is essential in optimizing methyl group supply and subsequent functioning of methyltransferase enzymes...
  6. Huang Y, Komoto J, Konishi K, Takata Y, Ogawa H, Gomi T, et al. Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes. J Mol Biol. 2000;298:149-62 pubmed
    Glycine N-methyltransferase (S-adenosyl-l-methionine: glycine methyltransferase, EC; GNMT) catalyzes the AdoMet-dependent methylation of glycine to form sarcosine (N-methylglycine)...
  7. Luka Z, Pakhomova S, Loukachevitch L, Egli M, Newcomer M, Wagner C. 5-methyltetrahydrofolate is bound in intersubunit areas of rat liver folate-binding protein glycine N-methyltransferase. J Biol Chem. 2007;282:4069-75 pubmed
    Glycine N-methyltransferase (GNMT) is a key regulatory enzyme in methyl group metabolism...
  8. Pattanayek R, Newcomer M, Wagner C. Crystal structure of apo-glycine N-methyltransferase (GNMT). Protein Sci. 1998;7:1326-31 pubmed
    The crystal structure of the recombinant apo-form of glycine N-methyltransferase (GNMT) has been determined at 2.5 A resolution...
  9. Fu Z, Hu Y, Konishi K, Takata Y, Ogawa H, Gomi T, et al. Crystal structure of glycine N-methyltransferase from rat liver. Biochemistry. 1996;35:11985-93 pubmed
    Glycine N-methyltransferase (GNMT) from rat liver is a tetrameric enzyme with 292 amino acid residues in each identical subunit and catalyzes the S-adenosylmethionine (AdoMet) dependent methylation of glycine to form sarcosine...
  10. Wei J, Zhang F, Zhang Y, Cao C, Li X, Li D, et al. Proteomic investigation of signatures for geniposide-induced hepatotoxicity. J Proteome Res. 2014;13:5724-33 pubmed publisher
    ..Two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate hepatic injuries significantly earlier than the current ..