Gabrg2

Summary

Gene Symbol: Gabrg2
Description: gamma-aminobutyric acid type A receptor gamma 2 subunit
Alias: gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, gamma-aminobutyric acid A receptor, gamma 2, gamma-aminobutyric acid (GABA) A receptor, gamma 2, gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
Species: rat
Products:     Gabrg2

Top Publications

  1. Seeburg P, Wisden W, Verdoorn T, Pritchett D, Werner P, Herb A, et al. The GABAA receptor family: molecular and functional diversity. Cold Spring Harb Symp Quant Biol. 1990;55:29-40 pubmed
  2. Liang C, Marks G. GABAA receptors are located in cholinergic terminals in the nucleus pontis oralis of the rat: implications for REM sleep control. Brain Res. 2014;1543:58-64 pubmed publisher
    ..Immunoreactive cholinergic boutons were found to be colocalized with GABA(A) receptor subunit protein ?2. This finding implicates a specific subtype and location of GABA(A) receptors in PnO of rat in the control of REM sleep. ..
  3. Eugène E, Depienne C, Baulac S, Baulac M, Fritschy J, Le Guern E, et al. GABA(A) receptor gamma 2 subunit mutations linked to human epileptic syndromes differentially affect phasic and tonic inhibition. J Neurosci. 2007;27:14108-16 pubmed
    ..Our data suggests that the phenotypic specificity of mutations affecting the GABA(A) receptor gamma2 gene may result from different actions specific to distinct modes of GABAergic signaling. ..
  4. Chandra D, Korpi E, Miralles C, De Blas A, Homanics G. GABAA receptor gamma 2 subunit knockdown mice have enhanced anxiety-like behavior but unaltered hypnotic response to benzodiazepines. BMC Neurosci. 2005;6:30 pubmed
    ..This suggests that reduced synaptic inhibition can lead to a phenotype of increased anxiety-like behavior. In contrast, normal drug effects can be maintained despite a dramatic reduction in GABAA-R targets. ..
  5. Mao X, Ma P, Cao D, Sun C, Ji Z, Min D, et al. Altered expression of GABAA receptors (?4, ?2 subunit), potassium chloride cotransporter 2 and astrogliosis in tremor rat hippocampus. Brain Res Bull. 2011;86:373-9 pubmed publisher
    ..These data demonstrate that altered expressions of GABA(A)R (?4, ?2) and KCC2 and astrogliosis observed in TRMs hippocampus may provide us good therapeutic targets for the treatment of genetic epilepsy. ..
  6. Khristy W, Ali N, Bravo A, de Leon R, Roy R, Zhong H, et al. Changes in GABA(A) receptor subunit gamma 2 in extensor and flexor motoneurons and astrocytes after spinal cord transection and motor training. Brain Res. 2009;1273:9-17 pubmed publisher
    ..Since the gamma2 subunit is involved with GABA(A) receptor trafficking and synaptic clustering, it appears that this subunit could be an important component of the activity-dependent response of the spinal cord after a spinal injury. ..
  7. Wang J, Liu S, Haditsch U, Tu W, Cochrane K, Ahmadian G, et al. Interaction of calcineurin and type-A GABA receptor gamma 2 subunits produces long-term depression at CA1 inhibitory synapses. J Neurosci. 2003;23:826-36 pubmed
    ..Thus, an activity-dependent physical and functional interaction between CaN and GABA(A) receptors is both necessary and sufficient for inducing LTD at CA1 individual inhibitory synapses. ..
  8. Jin H, Chiou T, Serwanski D, Miralles C, Pinal N, De Blas A. Ring finger protein 34 (RNF34) interacts with and promotes γ-aminobutyric acid type-A receptor degradation via ubiquitination of the γ2 subunit. J Biol Chem. 2014;289:29420-36 pubmed publisher
    ..The results indicate that RNF34 regulates postsynaptic γ2-GABAAR clustering and GABAergic synaptic innervation by interacting with and ubiquitinating the γ2-GABAAR subunit promoting GABAAR degradation. ..
  9. Draguhn A, Verdorn T, Ewert M, Seeburg P, Sakmann B. Functional and molecular distinction between recombinant rat GABAA receptor subtypes by Zn2+. Neuron. 1990;5:781-8 pubmed

More Information

Publications49

  1. Kneussel M, Haverkamp S, Fuhrmann J, Wang H, Wassle H, Olsen R, et al. The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse. Proc Natl Acad Sci U S A. 2000;97:8594-9 pubmed
    ..Consistent with this idea, a close homolog of GABARAP, p16, has been found to function as a late-acting intra-Golgi transport factor. ..
  2. Wisden W, Seeburg P. GABAA receptor channels: from subunits to functional entities. Curr Opin Neurobiol. 1992;2:263-9 pubmed
    ..In addition, molecular determinants underlying the differential binding properties of allosteric ligands to receptor subtypes have been identified. ..
  3. Shu H, Eisenman L, Jinadasa D, Covey D, Zorumski C, Mennerick S. Slow actions of neuroactive steroids at GABAA receptors. J Neurosci. 2004;24:6667-75 pubmed
  4. Saras A, Gisselmann G, Vogt Eisele A, Erlkamp K, Kletke O, Pusch H, et al. Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses. J Biol Chem. 2008;283:10470-5 pubmed publisher
    ..We show here for the first time that histamine modulates heteromultimeric GABA(A) receptors and may thus represent an endogenous ligand for an allosteric site. ..
  5. Frugier G, Coussen F, Giraud M, Odessa M, Emerit M, Boué Grabot E, et al. A gamma 2(R43Q) mutation, linked to epilepsy in humans, alters GABAA receptor assembly and modifies subunit composition on the cell surface. J Biol Chem. 2007;282:3819-28 pubmed
    ..This study reveals an involvement of the gamma2-Arg-43 domain in the control of receptor assembly that may be relevant to the effect of the heterozygous gamma2(R43Q) mutation leading to childhood absence epilepsy and febrile seizure. ..
  6. Li H, Huguenard J, Fisher R. Gender and age differences in expression of GABAA receptor subunits in rat somatosensory thalamus and cortex in an absence epilepsy model. Neurobiol Dis. 2007;25:623-30 pubmed
    ..The findings suggest a mechanism for the higher prevalence of absence epilepsy in female patients. ..
  7. Fu Z, Washbourne P, Ortinski P, Vicini S. Functional excitatory synapses in HEK293 cells expressing neuroligin and glutamate receptors. J Neurophysiol. 2003;90:3950-7 pubmed
  8. Han S, Youn D. GABAA receptor-mediated tonic currents in substantia gelatinosa neurons of rat spinal trigeminal nucleus pars caudalis. Neurosci Lett. 2008;441:296-301 pubmed publisher
    ..The GABAergic tonic inhibition demonstrated in this study may play a significant role in the sensory processing system of the Vc. ..
  9. Everitt A, Seymour V, Curmi J, Laver D, Gage P, Tierney M. Protein interactions involving the gamma2 large cytoplasmic loop of GABA(A) receptors modulate conductance. FASEB J. 2009;23:4361-9 pubmed publisher
  10. Dixon C, Sah P, Lynch J, Keramidas A. GABAA receptor ? and ? subunits shape synaptic currents via different mechanisms. J Biol Chem. 2014;289:5399-411 pubmed publisher
    ..Because this region determines post-synaptic localization, we hypothesize that GABAARs containing ?1 and ?2 use different mechanisms for synaptic clustering. ..
  11. Szczot M, Kisiel M, Czyzewska M, Mozrzymas J. ?1F64 Residue at GABA(A) receptor binding site is involved in gating by influencing the receptor flipping transitions. J Neurosci. 2014;34:3193-209 pubmed publisher
    ..In conclusion, we propose that the ?1F64 residue may participate in linking binding and gating by influencing flipping kinetics. ..
  12. Malherbe P, Sigel E, Baur R, Persohn E, Richards J, Mohler H. Functional characteristics and sites of gene expression of the alpha 1, beta 1, gamma 2-isoform of the rat GABAA receptor. J Neurosci. 1990;10:2330-7 pubmed
    ..g., mitral cells of the olfactory bulb, pyramidal cells of the hippocampus, and granule cells of the dentate gyrus and cerebellum. ..
  13. Tsuchiya H, Oka T, Nakamura K, Ichikawa A, Saper C, Sugimoto Y. Prostaglandin E2 attenuates preoptic expression of GABAA receptors via EP3 receptors. J Biol Chem. 2008;283:11064-71 pubmed publisher
    ..These results indicate that PGE(2)-EP3 signaling elicits G(i/o) activation in preoptic thermocenter neurons, and we propose the possibility that a rapid decrease in preoptic GABA(A) expression may be involved in PGE(2)-induced fever. ..
  14. Lovick T. Plasticity of GABAA receptor subunit expression during the oestrous cycle of the rat: implications for premenstrual syndrome in women. Exp Physiol. 2006;91:655-60 pubmed
  15. Saha S, Sieghart W, Fritschy J, McWilliam P, Batten T. Gamma-aminobutyric acid receptor (GABA(A)) subunits in rat nucleus tractus solitarii (NTS) revealed by polymerase chain reaction (PCR) and immunohistochemistry. Mol Cell Neurosci. 2001;17:241-57 pubmed
    ..These results suggest that ionotropic receptors comprising the alpha1, alpha3, beta2/3, and gamma2 may mediate inhibitory GABA responses in the NTS. ..
  16. Graf E, Zhang X, Jin S, Linhoff M, Craig A. Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins. Cell. 2004;119:1013-26 pubmed
  17. Danglot L, Triller A, Bessis A. Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Mol Cell Neurosci. 2003;23:264-78 pubmed
    ..Subsequently, the GABAAR gamma2 subunits associated with gephyrin at synaptic and/or extrasynaptic sites. Our data indicate that formation of GABAAR gamma2 subunit clusters is gephyrin independent. ..
  18. Ekema G, Zheng W, Lu L. Interaction of GABA receptor/channel rho(1) and gamma(2) subunit. Invest Ophthalmol Vis Sci. 2002;43:2326-33 pubmed
    ..Therefore, the functional role of GABA receptor/channels in the brain, retina, and spinal cord is more diversified because of the possible assembly between the GABA(A) gamma(2) subunit and GABA(C) rho(1) subunit. ..
  19. Kittler J, Chen G, Kukhtina V, Vahedi Faridi A, Gu Z, Tretter V, et al. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proc Natl Acad Sci U S A. 2008;105:3616-21 pubmed publisher
    ..These data define a mechanism for tyrosine kinase regulation of GABA(A)R surface levels and synaptic inhibition. ..
  20. Martire M, Altobelli D, Cannizzaro C, Maurizi S, Preziosi P. Prenatal diazepam exposure functionally alters the GABA(A) receptor that modulates [3H]noradrenaline release from rat hippocampal synaptosomes. Dev Neurosci. 2002;24:71-8 pubmed
  21. Eaton M, Lim Y, Bracamontes J, Steinbach J, Akk G. Agonist-specific conformational changes in the ?1-?2 subunit interface of the GABA A receptor. Mol Pharmacol. 2012;82:255-63 pubmed publisher
    ..We infer from the data that the structure of the ?1-?2 subunit interface undergoes agonist-specific conformational changes. ..
  22. Ramakrishnan L, Hess G. On the mechanism of a mutated and abnormally functioning gamma-aminobutyric acid (A) receptor linked to epilepsy. Biochemistry. 2004;43:7534-40 pubmed
    ..We discuss how a knowledge of the mechanism of the mutated receptor indicates an approach for alleviating this dysfunction. ..
  23. Frye C, Walf A. Activity of protein kinase C is important for 3alpha,5alpha-THP's actions at dopamine type 1-like and/or GABAA receptors in the ventral tegmental area for lordosis of rats. Brain Res Bull. 2008;77:91-7 pubmed publisher
    ..Effects on lordosis were not solely due to changes in general motor behavior. Thus, 3alpha,5alpha-THP's actions in the ventral tegmental area through membrane receptors may require activity of protein kinase C...
  24. Kananura C, Haug K, Sander T, Runge U, Gu W, Hallmann K, et al. A splice-site mutation in GABRG2 associated with childhood absence epilepsy and febrile convulsions. Arch Neurol. 2002;59:1137-41 pubmed
    Missense mutations in the GABRG2 gene, which encodes the gamma 2 subunit of central nervous gamma-aminobutyric acid (GABA)(A) receptors, have recently been described in 2 families with idiopathic epilepsy...
  25. Wang Q, Pless S, Lynch J. Ligand- and subunit-specific conformational changes in the ligand-binding domain and the TM2-TM3 linker of {alpha}1 {beta}2 {gamma}2 GABAA receptors. J Biol Chem. 2010;285:40373-86 pubmed publisher
    ..This result implies unexpected conformational mobility in this crucial part of the gating machinery. Together, this information provides new insights into the activation mechanisms of Cys-loop receptors. ..
  26. Smith K, Oliver P, Lumb M, Arancibia Carcamo I, Revilla Sanchez R, Brandon N, et al. Identification and characterisation of a Maf1/Macoco protein complex that interacts with GABAA receptors in neurons. Mol Cell Neurosci. 2010;44:330-41 pubmed publisher
    ..Expressing Macoco in neurons increases surface GABA(A)R levels. Our results suggest that Maf1 and Macoco are novel GABA(A)R interacting proteins important for regulating GABA(A)R surface expression and GABA(A)R signalling in the brain. ..
  27. Andrade S, Arbo B, Batista B, Neves A, Branchini G, Brum I, et al. Effect of progesterone on the expression of GABA(A) receptor subunits in the prefrontal cortex of rats: implications of sex differences and brain hemisphere. Cell Biochem Funct. 2012;30:696-700 pubmed publisher
  28. Bianchi M, Macdonald R. Slow phases of GABA(A) receptor desensitization: structural determinants and possible relevance for synaptic function. J Physiol. 2002;544:3-18 pubmed
    ..These results may indicate a previously unrecognized role for the slow phases of desensitization for synaptic function under conditions of repeated GABA(A) receptor activation. ..
  29. Boileau A, Pearce R, Czajkowski C. The short splice variant of the gamma 2 subunit acts as an external modulator of GABA(A) receptor function. J Neurosci. 2010;30:4895-903 pubmed publisher
  30. Wisden W, Laurie D, Monyer H, Seeburg P. The distribution of 13 GABAA receptor subunit mRNAs in the rat brain. I. Telencephalon, diencephalon, mesencephalon. J Neurosci. 1992;12:1040-62 pubmed
    ..Based on significant mismatches between alpha 4/delta and gamma mRNAs, we suggest that in vivo, the alpha 4 subunit contributes to GABAA receptors that lack BZ modulation. ..
  31. Turenius C, Charles J, Tsai D, Ebersole P, Htut M, Ngo P, et al. The tuberal lateral hypothalamus is a major target for GABAA--but not GABAB-mediated control of food intake. Brain Res. 2009;1283:65-72 pubmed publisher
    ..Collectively, our findings suggest that GABA(A) but not GABA(B) receptors in the tLH act to suppress feeding behavior. ..
  32. Facciolo R, Alo R, Canonaco M, Franzoni M. Early phylogenetic value of the major GABA(A) receptor subunit mRNAs in the telencephalon. Exp Brain Res. 2002;142:504-11 pubmed
  33. Laurie D, Seeburg P, Wisden W. The distribution of 13 GABAA receptor subunit mRNAs in the rat brain. II. Olfactory bulb and cerebellum. J Neurosci. 1992;12:1063-76 pubmed
    ..The delta-subunit mRNA appears to codistribute with alpha-subunit mRNAs (alpha 4 and alpha 6) associated with GABAA subunits that fail to bind benzodiazepine agonists. ..
  34. Muir J, Arancibia Carcamo I, Macaskill A, Smith K, Griffin L, Kittler J. NMDA receptors regulate GABAA receptor lateral mobility and clustering at inhibitory synapses through serine 327 on the ?2 subunit. Proc Natl Acad Sci U S A. 2010;107:16679-84 pubmed publisher
    ..Thus, NMDAR signaling, via calcineurin and a key GABA(A)R phosphorylation site, controls the stability of synaptic GABA(A)Rs, with important implications for activity-dependent control of synaptic inhibition and neuronal plasticity. ..
  35. Kharlamov E, Lepsveridze E, Meparishvili M, Solomonia R, Lu B, Miller E, et al. Alterations of GABA(A) and glutamate receptor subunits and heat shock protein in rat hippocampus following traumatic brain injury and in posttraumatic epilepsy. Epilepsy Res. 2011;95:20-34 pubmed publisher
    ..014, and p=0.005, respectively); no changes were found in HSP90 expression. These results provide for the first time evidence of long-term alterations of GABA(A) and glutamate receptor subunits and a HSP following CCI. ..
  36. Kang J, Shen W, Macdonald R. The GABRG2 mutation, Q351X, associated with generalized epilepsy with febrile seizures plus, has both loss of function and dominant-negative suppression. J Neurosci. 2009;29:2845-56 pubmed publisher
    ..However, heterozygous gamma2(+/-) gene deletion mice are seizure free, suggesting that the loss of one GABRG2 allele alone in heterozygous patients may not be sufficient to produce epilepsy...
  37. Li X, Dong L, Liu Z, Luo J. Expression of gamma-aminobutyric acid A receptor subunits alpha1, beta1, gamma2 mRNA in rats with hepatic encephalopathy. World J Gastroenterol. 2005;11:3319-22 pubmed
  38. Li R, Yu W, Christie S, Miralles C, Bai J, LoTurco J, et al. Disruption of postsynaptic GABA receptor clusters leads to decreased GABAergic innervation of pyramidal neurons. J Neurochem. 2005;95:756-70 pubmed
    ..The results indicate that the postsynaptic clustering of GABA(A)Rs in pyramidal neurons is involved in the stabilization of the presynaptic GABAergic contacts. ..
  39. Fritschy J, Johnson D, Mohler H, Rudolph U. Independent assembly and subcellular targeting of GABA(A)-receptor subtypes demonstrated in mouse hippocampal and olfactory neurons in vivo. Neurosci Lett. 1998;249:99-102 pubmed
    ..These findings indicate that the assembly of distinct GABA(A)-receptor subtypes in the same neuron is regulated independently. Furthermore, the alpha-subunit is a prime candidate for providing domains which direct subcellular targeting. ..
  40. Shivers B, Killisch I, Sprengel R, Sontheimer H, Kohler M, Schofield P, et al. Two novel GABAA receptor subunits exist in distinct neuronal subpopulations. Neuron. 1989;3:327-37 pubmed
    ..These findings have implications for the composition of two different GABAA receptor subtypes and for information processing in networks using GABA for signaling. ..