Gene Symbol: Ehmt2
Description: euchromatic histone lysine methyltransferase 2
Alias: Bat8, G9a, Ng36, histone-lysine N-methyltransferase EHMT2, HLA-B associated transcript 8, rat orthologue, euchromatic histone-lysine N-methyltransferase 2, histone-lysine N-methyltransferase, H3 lysine-9 specific 3
Species: rat
Products:     Ehmt2

Top Publications

  1. Tachibana M, Ueda J, Fukuda M, Takeda N, Ohta T, Iwanari H, et al. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev. 2005;19:815-26 pubmed
    Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis...
  2. Chen M, Hua K, Kao H, Chi C, Wei L, Johansson G, et al. H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM. Cancer Res. 2010;70:7830-40 pubmed publisher
    b>G9a is a mammalian histone methyltransferase that contributes to the epigenetic silencing of tumor suppressor genes...
  3. Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, et al. Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas. Am J Dermatopathol. 2014;36:211-6 pubmed publisher
    ..Here, we used immunohistochemistry to examine the expression of the H3K9 methyltransferases, EHMT2 and SETDB1, and a H3K9 demethylase, LSD1, in 67 patients with melanoma...
  4. de Vogel S, Wouters K, Gottschalk R, Van Schooten F, de Goeij A, De Bruine A, et al. Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer. Cancer Epidemiol Biomarkers Prev. 2009;18:3086-96 pubmed publisher
    ..metabolizing enzymes (MTHFR, MTR, and MTRR), DNA methyltransferase DNMT3b, and histone methyltransferases (EHMT1, EHMT2, and PRDM2), with colorectal cancers, with or without the CpG island methylator phenotype (CIMP), MLH1 ..
  5. Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, et al. The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev. 2014;28:317-27 pubmed publisher
    ..Here we establish the histone methyltransferase G9a/EHMT2 as a selective regulator of fast proliferating myeloid progenitors with no discernible function in hematopoietic ..
  6. Zimmermann N, Zschocke J, Perisic T, Yu S, Holsboer F, Rein T. Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels. Biochem J. 2012;448:93-102 pubmed publisher
    ..putative DNMT1-inhibitory mechanisms and discovered that a known stimulator of DNMT1, the histone methyltransferase G9a, exhibited decreased protein levels and interactions with DNMT1 upon AD exposure...
  7. Zhao M, Liang G, Wu X, Wang S, Zhang P, Su Y, et al. Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 2012;166:226-73 pubmed publisher
    ..EP300), histone deacetylase 1 (HDAC1), myeloid/lymphoid or mixed lineage leukemia (MLL), SET7/9 (SETD7), G9A (EHMT2), JMJD2C (KDM4C) and JARID1A (KDM5A) expression, as well as reduced HDAC2, HDAC7, LSD1 (KDM1A), JMJD2A (KDM4A) and ..
  8. Huang J, Dorsey J, Chuikov S, Pérez Burgos L, Zhang X, Jenuwein T, et al. G9a and Glp methylate lysine 373 in the tumor suppressor p53. J Biol Chem. 2010;285:9636-41 pubmed publisher
    ..describe a novel lysine methylation site in p53 that is carried out by two homologous histone methyltransferases, G9a and Glp. G9a and Glp specifically methylate p53 at Lys(373), resulting mainly in dimethylation...
  9. Tachibana M, Nozaki M, Takeda N, Shinkai Y. Functional dynamics of H3K9 methylation during meiotic prophase progression. EMBO J. 2007;26:3346-59 pubmed
    ..b>G9a is a major mammalian H3K9 methyltransferase at euchromatin and is essential for mouse embryogenesis...

More Information


  1. Ikegami K, Iwatani M, Suzuki M, Tachibana M, Shinkai Y, Tanaka S, et al. Genome-wide and locus-specific DNA hypomethylation in G9a deficient mouse embryonic stem cells. Genes Cells. 2007;12:1-11 pubmed
    ..b>G9a is a euchromatin-localized histone methyltransferase (HMT) and catalyzes methylation of histone H3 at lysines 9 and ..
  2. Tachibana M, Matsumura Y, Fukuda M, Kimura H, Shinkai Y. G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J. 2008;27:2681-90 pubmed publisher
    ..b>G9a and GLP/Eu-HMTase1 are two-related mammalian lysine methyltransferases and a G9a/GLP heteromeric complex regulates ..
  3. Zhong X, Chen X, Guan X, Zhang H, Ma Y, Zhang S, et al. Overexpression of G9a and MCM7 in oesophageal squamous cell carcinoma is associated with poor prognosis. Histopathology. 2015;66:192-200 pubmed publisher
    Histone methyltransferase G9a has been primarily understood as a co-repressor of gene expression, but it has been shown that G9a also positively regulates nuclear receptor-mediated transcription...
  4. Trojer P, Zhang J, Yonezawa M, Schmidt A, Zheng H, Jenuwein T, et al. Dynamic Histone H1 Isotype 4 Methylation and Demethylation by Histone Lysine Methyltransferase G9a/KMT1C and the Jumonji Domain-containing JMJD2/KDM4 Proteins. J Biol Chem. 2009;284:8395-405 pubmed publisher
    ..Here we report that the euchromatic histone lysine methyltransferase G9a/KMT1C mediates H1...
  5. Benlhabib H, Mendelson C. Epigenetic regulation of surfactant protein A gene (SP-A) expression in fetal lung reveals a critical role for Suv39h methyltransferases during development and hypoxia. Mol Cell Biol. 2011;31:1949-58 pubmed publisher
  6. Ueda J, Tachibana M, Ikura T, Shinkai Y. Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP. J Biol Chem. 2006;281:20120-8 pubmed
    b>G9a is a SET-domain mammalian histone methyltransferase responsible for mono- and dimethylation of lysine 9 in histone H3 (H3K9) at euchromatic regions...
  7. Wu R, Terry A, Singh P, Gilbert D. Differential subnuclear localization and replication timing of histone H3 lysine 9 methylation states. Mol Biol Cell. 2005;16:2872-81 pubmed
    ..Disruption of the Suv39h1,2 or G9a methyltransferases in murine embryonic stem cells resulted in a redistribution of methyl epitopes, but did not ..
  8. Gupta Agarwal S, Franklin A, Deramus T, Wheelock M, Davis R, McMahon L, et al. G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation. J Neurosci. 2012;32:5440-53 pubmed publisher
    ..Here, we identify an essential role for the G9a/G9a-like protein (GLP) lysine dimethyltransferase complex and the histone H3 lysine 9 dimethylation (H3K9me2) marks ..
  9. Xin Z, Tachibana M, Guggiari M, Heard E, Shinkai Y, Wagstaff J. Role of histone methyltransferase G9a in CpG methylation of the Prader-Willi syndrome imprinting center. J Biol Chem. 2003;278:14996-5000 pubmed
    ..we show that histone H3 Lys-9 methylation of the PWS-IC is reduced in mouse embryonic stem (ES) cells lacking the G9a histone H3 Lys-9/Lys-27 methyltransferase and that maintenance of CpG methylation of the PWS-IC in mouse ES cells ..
  10. Bekdash R, Zhang C, Sarkar D. Gestational choline supplementation normalized fetal alcohol-induced alterations in histone modifications, DNA methylation, and proopiomelanocortin (POMC) gene expression in ?-endorphin-producing POMC neurons of the hypothalamus. Alcohol Clin Exp Res. 2013;37:1133-42 pubmed publisher
    ..In addition, we tested whether gestational choline supplementation prevents the adverse effects of EtOH on these neurons...
  11. Maze I, Chaudhury D, Dietz D, von Schimmelmann M, Kennedy P, Lobo M, et al. G9a influences neuronal subtype specification in striatum. Nat Neurosci. 2014;17:533-9 pubmed publisher
    Cocaine-mediated repression of the histone methyltransferase (HMT) G9a has recently been implicated in transcriptional, morphological and behavioral responses to chronic cocaine administration...
  12. Zhang Y, Chen S, Laumet G, Chen H, Pan H. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-mediated Transcriptional Repression of μ-Opioid Receptors in Primary Sensory Neurons. J Biol Chem. 2016;291:8475-85 pubmed publisher
    ..G9a (encoded by Ehmt2), a histone 3 at lysine 9 methyltransferase, is a key chromatin regulator responsible for gene silencing...
  13. Coward W, Feghali Bostwick C, Jenkins G, Knox A, Pang L. A central role for G9a and EZH2 in the epigenetic silencing of cyclooxygenase-2 in idiopathic pulmonary fibrosis. FASEB J. 2014;28:3183-96 pubmed publisher
    ..This study explored the role of G9a- and enhancer of zeste homolog 2 (EZH2)-mediated methylation of histone H3 lysine 9 (H3K9me3) and histone H3 lysine ..
  14. Thienpont B, Aronsen J, Robinson E, Okkenhaug H, Loche E, Ferrini A, et al. The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy. J Clin Invest. 2017;127:335-348 pubmed publisher
    ..H3K9me2 was reduced following a miR-217-mediated decrease in expression of the H3K9 dimethyltransferases EHMT1 and EHMT2 (EHMT1/2)...
  15. Anderson A, Carter K, Anderson D, Wise M. Coexpression of nuclear receptors and histone methylation modifying genes in the testis: implications for endocrine disruptor modes of action. PLoS ONE. 2012;7:e34158 pubmed publisher
  16. Yu Y, Song C, Zhang Q, DiMaggio P, Garcia B, York A, et al. Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA. Mol Cell. 2012;46:7-17 pubmed publisher
    ..presence of H3K56 monomethylation (H3K56me1) in mammalian cells and find that the histone lysine methytransferase G9a/KMT1C is required for H3K56me1 both in vivo and in vitro...
  17. Davison J, Mellott T, Kovacheva V, Blusztajn J. Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain. J Biol Chem. 2009;284:1982-9 pubmed publisher
    ..The mRNA and protein expression of histone methyltransferases G9a and Suv39h1 were directly related to the availability of choline...
  18. Yuan X, Feng W, Imhof A, Grummt I, Zhou Y. Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a. Mol Cell. 2007;27:585-95 pubmed
    ..Here we show that a complex consisting of CSB, RNA polymerase I (Pol I), and histone methyltransferase G9a is present at active rRNA genes...
  19. Artal Martinez de Narvajas A, Gomez T, Zhang J, Mann A, Taoda Y, Gorman J, et al. Epigenetic regulation of autophagy by the methyltransferase G9a. Mol Cell Biol. 2013;33:3983-93 pubmed publisher
    ..Here we show that the methyltransferase G9a coordinates the transcriptional activation of key regulators of autophagosome formation by remodeling the chromatin ..
  20. Chen G, Wang X, Zhang Y, Ru X, Zhou L, Tian Y. H3K9 histone methyltransferase G9a ameliorates dilated cardiomyopathy via the downregulation of cell adhesion molecules. Mol Med Rep. 2015;11:3872-9 pubmed publisher
    ..H3K9 histone methyltransferase G9a has been previously characterized, although its functions in DCM are not yet understood...
  21. Ohhata T, Tachibana M, Tada M, Tada T, Sasaki H, Shinkai Y, et al. X-inactivation is stably maintained in mouse embryos deficient for histone methyl transferase G9a. Genesis. 2004;40:151-6 pubmed
    ..We previously showed that G9a is one of the histone methyltransferases specific for H3 Lys-9 and is essential for embryonic development...