Cyp24a1

Summary

Gene Symbol: Cyp24a1
Description: cytochrome P450, family 24, subfamily a, polypeptide 1
Alias: 24-OHase, 24R-Ohase, Cyp24, P450-CC24, VITD12, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial, 25-hydroxyvitamin D3 24-hydroxylase, 25-hydroxyvitaminD324-hydroxylase, Cytochrom P450 (25-hydroxyvitamin D24-hydroxylase), cytochrome P450 24A1, cytochrome P450, subfamily 24, cytochrome P450-CC24, vitamin D(3) 24-hydroxylase
Species: rat
Products:     Cyp24a1

Top Publications

  1. Sawada N, Kusudo T, Sakaki T, Hatakeyama S, Hanada M, Abe D, et al. Novel metabolism of 1 alpha,25-dihydroxyvitamin D3 with C24-C25 bond cleavage catalyzed by human CYP24A1. Biochemistry. 2004;43:4530-7 pubmed
    Our previous study revealed that human CYP24A1 catalyzes a remarkable metabolism consisting of both C-23 and C-24 hydroxylation pathways that used both 25(OH)D(3) and 1alpha,25(OH)(2)D(3) as substrates, while rat CYP24A1 showed extreme ..
  2. Tieu E, Li W, Chen J, Kim T, Ma D, Slominski A, et al. Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1. J Steroid Biochem Mol Biol. 2015;149:153-65 pubmed publisher
    ..A previous study indicated 20(OH)D3 can be metabolized by rat CYP24A1 to products including 20S,24-dihydroxyvitamin D3 [20,24(OH)2D3] and 20S,25-dihydroxyvitamin D3, with both ..
  3. Zhou Y, Liang Z, Guo Y, Zhang H, Wang K. High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96. Mol Cell Endocrinol. 2015;404:75-81 pubmed publisher
    ..High glucose upregulated the expression of CYP24A1 protein, which catalyzes the conversion of 1,25(OH)2D3 into inactive products, further impairing the ability of 1,..
  4. Matsuzaki H, Katsumata S, Kajita Y, Miwa M. Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats. Magnes Res. 2013;26:83-6 pubmed publisher
    ..These results suggest that Mg deficiency induces downregulation of 1?(OH)ase and type IIa and IIc Na/Pi cotransporters, and upregulation of 24(OH)ase in the kidney. ..
  5. Anderson P, O Loughlin P, May B, Morris H. Quantification of mRNA for the vitamin D metabolizing enzymes CYP27B1 and CYP24 and vitamin D receptor in kidney using real-time reverse transcriptase- polymerase chain reaction. J Mol Endocrinol. 2003;31:123-32 pubmed
    ..regulation of three genes, 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1), 25-hydroxyvitamin D-24-hydroxylase (CYP24) and vitamin D receptor (VDR)...
  6. Vigano P, Lattuada D, Mangioni S, Ermellino L, Vignali M, Caporizzo E, et al. Cycling and early pregnant endometrium as a site of regulated expression of the vitamin D system. J Mol Endocrinol. 2006;36:415-24 pubmed
    ..interleukin (IL)-1beta (50 and 500 pg/ml) while addition of the active form of the hormone could modulate both CYP24 and osteopontin gene expression...
  7. Ohyama Y, Noshiro M, Okuda K. Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase. FEBS Lett. 1991;278:195-8 pubmed
    ..Using the cDNA as a probe we demonstrated that the increase of 24-hydroxylation activity caused by administration of vitamin D3 into rats was accompanied by an increase of the mRNA. ..
  8. Chen K, Prahl J, DeLuca H. Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA. Proc Natl Acad Sci U S A. 1993;90:4543-7 pubmed
    ..Transient levels of 24-hydroxylase activity were measured in transfected mammalian COS-1 cells and in recombinant baculovirus-infected Spodoptera frugiperda (Sf21) insect cells. ..
  9. Dwivedi P, Hii C, Ferrante A, Tan J, Der C, Omdahl J, et al. Role of MAP kinases in the 1,25-dihydroxyvitamin D3-induced transactivation of the rat cytochrome P450C24 (CYP24) promoter. Specific functions for ERK1/ERK2 and ERK5. J Biol Chem. 2002;277:29643-53 pubmed
    ..1,25-dihydroxyvitamin D(3) (1,25D) at the genomic and signal transduction levels to induce rat cytochrome P450C24 (CYP24) gene expression...

Scientific Experts

More Information

Publications26

  1. Kasuga H, Hosogane N, Matsuoka K, Mori I, Sakura Y, Shimakawa K, et al. Characterization of transgenic rats constitutively expressing vitamin D-24-hydroxylase gene. Biochem Biophys Res Commun. 2002;297:1332-8 pubmed
    Vitamin D-24-hydroxylase (CYP24) is one of the enzymes responsible for vitamin D metabolism. CYP24 catalyzes the conversion of 25-hydroxyvitamin D(3) [25(OH)D(3)] to 24,25-dihydroxyvitamin D(3) [24,25(OH)(2)D(3)] in the kidney...
  2. Nutchey B, Kaplan J, Dwivedi P, Omdahl J, Ferrante A, May B, et al. Molecular action of 1,25-dihydroxyvitamin D3 and phorbol ester on the activation of the rat cytochrome P450C24 (CYP24) promoter: role of MAP kinase activities and identification of an important transcription factor binding site. Biochem J. 2005;389:753-62 pubmed
    Although investigations of the transcriptional regulation of the rat cytochrome P450C24 [CYP24 (25-hydroxyvitamin D3 24-hydroxylase)] gene by 1,25D (1,25-dihydroxyvitamin D3) at either the genomic, or more recently at the non-genomic, ..
  3. Hahn C, Kerry D, Omdahl J, May B. Identification of a vitamin D responsive element in the promoter of the rat cytochrome P450(24) gene. Nucleic Acids Res. 1994;22:2410-6 pubmed
    ..A VDRE at position -150 to -136 in the promoter of the rat P450(24) gene is identified in this study and found to be important in mediating the enhanced expression of the gene by 1,25-(OH)2D3. ..
  4. Vuica A, Vukojevic K, Ferhatović Hamzić L, Jeric M, Puljak L, Grkovic I, et al. Expression pattern of CYP24 in liver during ageing in long-term diabetes. Acta Histochem. 2016;118:486-95 pubmed publisher
    ..be a potential link between ageing/diabetes mellitus (DM) and bone loss, we studied the dynamic of expression of CYP24, the main calcitriol catabolising enzyme in the liver of rats during ageing and a long-term experimental DM1...
  5. Fischer D, Thomé M, Becker S, Cordes T, Diedrich K, Friedrich M, et al. Expression of 25-hydroxyvitamin D3-24-hydroxylase in benign and malignant ovarian cell lines and tissue. Anticancer Res. 2009;29:3635-9 pubmed
    ..01). In the malignant ovarian tissue, the expression was significantly higher in RNA (p<0.001), but lower at the protein level (p<0.01). An increased 24-OHase expression could indicate a decrease in available calcitriol. ..
  6. St Arnaud R, Messerlian S, Moir J, Omdahl J, Glorieux F. The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the pseudovitamin D-deficiency rickets (PDDR) disease locus. J Bone Miner Res. 1997;12:1552-9 pubmed
    ..We have used a probe derived from the rat 25-hydroxyvitamin D-24-hydroxylase (CYP24; 24-OHase) sequence to identify and clone the 1alpha-OHase cDNA. The full-length 1alpha-OHase clone of 2...
  7. Masuda S, Kaufmann M, Byford V, Gao M, St Arnaud R, Arabian A, et al. Insights into Vitamin D metabolism using cyp24 over-expression and knockout systems in conjunction with liquid chromatography/mass spectrometry (LC/MS). J Steroid Biochem Mol Biol. 2004;89-90:149-53 pubmed
    ..Our studies focused on: (1) elucidating the role of CYP24 in 25-OH-D3 and 1alpha,25-(OH)2D3 metabolism; (2) exploring how DBP influences this process; (3) measuring 25-OH-..
  8. van Driel M, Koedam M, Buurman C, Hewison M, Chiba H, Uitterlinden A, et al. Evidence for auto/paracrine actions of vitamin D in bone: 1alpha-hydroxylase expression and activity in human bone cells. FASEB J. 2006;20:2417-9 pubmed
    ..to modulate osteoblast activity, resulting in induced alkaline phosphatase (ALP) activity, osteocalcin (OC) and CYP24 mRNA expression, and mineralization...
  9. Gao X, Dwivedi P, Omdahl J, Morris H, May B. Calcitonin stimulates expression of the rat 25-hydroxyvitamin D3-24-hydroxylase (CYP24) promoter in HEK-293 cells expressing calcitonin receptor: identification of signaling pathways. J Mol Endocrinol. 2004;32:87-98 pubmed
    Regulation of the gene for renal 25-hydroxyvitamin D-24-hydroxylase (CYP24) is important for controlling the level of circulating 1,25-dihydroxyvitamin D3 (1,25(OH)2D3)...
  10. Annalora A, Goodin D, Hong W, Zhang Q, Johnson E, Stout C. Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism. J Mol Biol. 2010;396:441-51 pubmed publisher
    Cytochrome P450 (CYP) 24A1 catalyzes the side-chain oxidation of the hormonal form of vitamin D. Expression of CYP24A1 is up-regulated to attenuate vitamin D signaling associated with calcium homeostasis and cellular growth processes...
  11. Wang L, Gao Z, Wang L, Gao Y. Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats. Nephrology (Carlton). 2016;21:1063-1068 pubmed publisher
    Abnormal upregulation of CYP24 contributes to vitamin D insufficiency and resistance to vitamin D therapy in chronic kidney disease (CKD), because human CYP24 is a key enzyme involved in the inactivation of 1a,25-dihydroxyvitamin D3 (1a,..
  12. Ohyama Y, Ozono K, Uchida M, Shinki T, Kato S, Suda T, et al. Identification of a vitamin D-responsive element in the 5'-flanking region of the rat 25-hydroxyvitamin D3 24-hydroxylase gene. J Biol Chem. 1994;269:10545-50 pubmed
  13. Hamamoto H, Kusudo T, Urushino N, Masuno H, Yamamoto K, Yamada S, et al. Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats. Mol Pharmacol. 2006;70:120-8 pubmed
    Our previous studies revealed the species-based difference of CYP24A1-dependent vitamin D metabolism. Although human CYP24A1 catalyzes both C-23 and C-24 oxidation pathways, rat CYP24A1 shows almost no C-23 oxidation pathway...
  14. Nehring J, Zierold C, DeLuca H. Lithocholic acid can carry out in vivo functions of vitamin D. Proc Natl Acad Sci U S A. 2007;104:10006-9 pubmed
    ..Further, LCA induces CYP24-hydroxylase mRNA gene expression in the kidney of vitamin D-deficient rats...
  15. Takemoto F, Shinki T, Yokoyama K, Inokami T, Hara S, Yamada A, et al. Gene expression of vitamin D hydroxylase and megalin in the remnant kidney of nephrectomized rats. Kidney Int. 2003;64:414-20 pubmed
    ..nephrectomized rats, we examined changes in mRNA levels of CYP27B1 (25-hydroxyvitamin D3-1 alpha-hydroxylase), CYP24 (25-hydroxyvitamin D3-24-hydroxylase), and vitamin D receptor in relation to megalin, recently found to ..
  16. Ohyama Y, Noshiro M, Eggertsen G, Gotoh O, Kato Y, Bjorkhem I, et al. Structural characterization of the gene encoding rat 25-hydroxyvitamin D3 24-hydroxylase. Biochemistry. 1993;32:76-82 pubmed
    ..The structure of the gene is discussed in relation to present knowledge about the mechanism of regulation of the 25-hydroxyvitamin D3 24-hydroxylase and calcium homeostasis. ..
  17. Kusudo T, Sakaki T, Abe D, Fujishima T, Kittaka A, Takayama H, et al. Metabolism of A-ring diastereomers of 1alpha,25-dihydroxyvitamin D3 by CYP24A1. Biochem Biophys Res Commun. 2004;321:774-82 pubmed
    ..1beta,3alpha), was examined to compare the substrate specificity and reaction specificity of CYP24A1 between humans and rats...