Cyp11b2

Summary

Gene Symbol: Cyp11b2
Description: cytochrome P450, family 11, subfamily b, polypeptide 2
Alias: Cp45as, Cyp11b3, RNCP45AS, cytochrome P450 11B2, mitochondrial, CYPXIB2, CYPXIB3, Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), P450-Aldo-1, P450-Aldo-2, aldosterone synthase, cytochrome P450 11B3, mitochondrial, cytochrome P450, subfamily 11B, polypeptide 2, cytochrome P450-Aldo-1, cytochrome P450-Aldo-2, steroid 11-beta-hydroxylase
Species: rat
Products:     Cyp11b2

Top Publications

  1. Ye P, Kenyon C, MacKenzie S, Seckl J, Fraser R, Connell J, et al. Regulation of aldosterone synthase gene expression in the rat adrenal gland and central nervous system by sodium and angiotensin II. Endocrinology. 2003;144:3321-8 pubmed
    ..a highly sensitive QRT-PCR method for the measurement of CYP11B1 (11beta-hydroxylase) and CYP11B2 (aldosterone synthase) mRNAs to study their expression in the rat brain in response to dietary sodium manipulation and ..
  2. MacKenzie S, Lai M, Clark C, Fraser R, Gomez Sanchez C, Seckl J, et al. 11beta-hydroxylase and aldosterone synthase expression in fetal rat hippocampal neurons. J Mol Endocrinol. 2002;29:319-25 pubmed
    ..from the fetal rat can perform the biochemical reactions associated with the enzymes 11beta-hydroxylase and aldosterone synthase. Here, we demonstrate directly that these enzymes are present within primary cultures of fetal rat ..
  3. Ye P, Kenyon C, MacKenzie S, Jong A, Miller C, Gray G, et al. The aldosterone synthase (CYP11B2) and 11beta-hydroxylase (CYP11B1) genes are not expressed in the rat heart. Endocrinology. 2005;146:5287-93 pubmed
    b>Aldosterone synthase (CYP11B2) and 11beta-hydroxylase (CYP11B1) catalyze the production of aldosterone and corticosterone, respectively, in the rat adrenal cortex...
  4. Xue C, Siragy H. Local renal aldosterone system and its regulation by salt, diabetes, and angiotensin II type 1 receptor. Hypertension. 2005;46:584-90 pubmed
    b>CYP11B2 is the enzyme responsible for aldosterone synthesis mainly in the adrenal gland...
  5. Kupari M, Hautanen A, Lankinen L, Koskinen P, Virolainen J, Nikkila H, et al. Associations between human aldosterone synthase (CYP11B2) gene polymorphisms and left ventricular size, mass, and function. Circulation. 1998;97:569-75 pubmed
    ..Such variations might be associated with polymorphisms in the gene encoding aldosterone synthase (CYP11B2), the enzyme catalyzing the last steps of aldosterone biosynthesis...
  6. Pascoe L, Curnow K, Slutsker L, Rosler A, White P. Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency. Proc Natl Acad Sci U S A. 1992;89:4996-5000 pubmed
    ..It is genetically linked to the CYP11B1 and CYP11B2 genes that, respectively, encode two cytochrome P450 isozymes, P450XIB1 and P450XIB2...
  7. Tran P, Georgieff M, Engeland W. Sodium depletion increases sympathetic neurite outgrowth and expression of a novel TMEM35 gene-derived protein (TUF1) in the rat adrenal zona glomerulosa. Endocrinology. 2010;151:4852-60 pubmed publisher
    ..to 1 wk sodium restriction, VIP and NPY fibers elongated in parallel with expansion of the ZG, shown by aldosterone synthase (AS) expression, but calcitonin gene-related peptide fibers were not affected...
  8. Prasad P, Tiwari A, Kumar K, Ammini A, Gupta A, Gupta R, et al. Chronic renal insufficiency among Asian Indians with type 2 diabetes: I. Role of RAAS gene polymorphisms. BMC Med Genet. 2006;7:42 pubmed
    ..angiotensinogen (ATG), angiotensin converting enzyme I (ACE), angiotensin II type 1 receptor (AT1) and aldosterone synthase (CYP11B2) gene from the RAAS pathway and one from chymase pathway were genotyped using polymerase chain ..
  9. Giuliani L, Lenzini L, Antonello M, Aldighieri E, Belloni A, Fassina A, et al. Expression and functional role of urotensin-II and its receptor in the adrenal cortex and medulla: novel insights for the pathophysiology of primary aldosteronism. J Clin Endocrinol Metab. 2009;94:684-90 pubmed publisher
    ..007) the adrenocortical expression of immunoreactive aldosterone synthase. Urotensin II is a putative mediator of the effects of the adrenal medulla and pheochromocytoma on the ..

More Information

Publications53

  1. Russo P, Lauria F, Loguercio M, Barba G, Arnout J, Cappuccio F, et al. -344C/T Variant in the promoter of the aldosterone synthase gene (CYP11B2) is associated with metabolic syndrome in men. Am J Hypertens. 2007;20:218-22 pubmed
    The -344C/T variant in the promoter of the aldosterone synthase gene (CYP11B2) has been associated with hypertension and may influence glucose homeostasis and body mass in humans...
  2. Shojaati K, Causevic M, Kadereit B, Dick B, Imobersteg J, Schneider H, et al. Evidence for compromised aldosterone synthase enzyme activity in preeclampsia. Kidney Int. 2004;66:2322-8 pubmed
    ..for gas chromatography-mass spectroscopic measurements of steroid hormones reflecting apparent Aldo synthase (CYP11B2) and 11-hydroxylase (CYP11B1) activities...
  3. Lee S, Yoo T, Nam B, Kim D, Li J, Jung D, et al. Activation of local aldosterone system within podocytes is involved in apoptosis under diabetic conditions. Am J Physiol Renal Physiol. 2009;297:F1381-90 pubmed publisher
    ..b>Aldosterone synthase (CYP11B2) and MCR mRNA and protein expression were determined by real-time PCR and Western blot, ..
  4. Gomez Sanchez E, Gomez Sanchez C, Plonczynski M, Gomez Sanchez C. Aldosterone synthesis in the brain contributes to Dahl salt-sensitive rat hypertension. Exp Physiol. 2010;95:120-30 pubmed publisher
    ..We used real time RT-PCR to measure mRNA of aldosterone synthase and 11beta-hydroxylase, the requisite enzymes for the last step in the synthesis of aldosterone and ..
  5. Ranade K, Wu K, Risch N, Olivier M, Pei D, Hsiao C, et al. Genetic variation in aldosterone synthase predicts plasma glucose levels. Proc Natl Acad Sci U S A. 2001;98:13219-24 pubmed
    ..found, however, highly significant association between single-nucleotide polymorphisms in the aldosterone synthase gene and plasma glucose levels in a large population of Chinese and Japanese origin...
  6. Ogishima T, Mitani F, Ishimura Y. Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex. J Biol Chem. 1989;264:10935-8 pubmed
  7. White P, Dupont J, New M, Leiberman E, Hochberg Z, Rosler A. A mutation in CYP11B1 (Arg-448----His) associated with steroid 11 beta-hydroxylase deficiency in Jews of Moroccan origin. J Clin Invest. 1991;87:1664-7 pubmed
    ..encoded by the CYP11B1 gene which is located on chromosome 8q22 along with a homologous gene of unknown function, CYP11B2. To identify mutations in CYP11B1 associated with 11 beta-hydroxylase deficiency in Moroccan Jews, ..
  8. Matsukawa N, Nonaka Y, Ying Z, Higaki J, Ogihara T, Okamoto M. Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta). Biochem Biophys Res Commun. 1990;169:245-52 pubmed
    Two distinct forms of cDNA encoding rat aldosterone synthase were cloned from an adrenal capsular tissue cDNA library...
  9. Bibeau K, Battista M, Houde V, Brochu M. Fetal adrenal gland alterations in a rat model of adverse intrauterine environment. Am J Physiol Regul Integr Comp Physiol. 2010;298:R899-911 pubmed publisher
    ..associated with higher mRNA adrenal levels of angiotensin II receptor type 1 (AT(1)R) and cytochrome P450 aldosterone synthase in response to decreased serum sodium content...
  10. Imai M, Shimada H, Okada Y, Matsushima Hibiya Y, Ogishima T, Ishimura Y. Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex. FEBS Lett. 1990;263:299-302 pubmed
    Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem...
  11. Fujisawa G, Muto S, Okada K, Kusano E, Ishibashi S. Mineralocorticoid receptor antagonist spironolactone prevents pig serum-induced hepatic fibrosis in rats. Transl Res. 2006;148:149-56 pubmed
    ..actin (alpha-SMA)-positive hepatic stellate cells (HSCs), Na/H exchange isoform-1 (NHE-1) protein, CYP11B2 aldosterone synthase protein for liver tissues, and plasma aldosterone concentrations were compared among the 3 groups of rats...
  12. Zhou M, Gomez Sanchez C. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem Biophys Res Commun. 1993;194:112-7 pubmed
    A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library...
  13. Huang B, White R, Ahmad M, Tan J, Jeng A, Leenen F. Central infusion of aldosterone synthase inhibitor attenuates left ventricular dysfunction and remodelling in rats after myocardial infarction. Cardiovasc Res. 2009;81:574-81 pubmed publisher
    ..ligation, Wistar rats received an intra-cerebroventricular (icv) infusion via osmotic mini-pumps of the aldosterone synthase inhibitor FAD286 at 100 microg/kg/day or vehicle for 4 weeks...
  14. Huang B, White R, Ahmad M, Jeng A, Leenen F. Central infusion of aldosterone synthase inhibitor prevents sympathetic hyperactivity and hypertension by central Na+ in Wistar rats. Am J Physiol Regul Integr Comp Physiol. 2008;295:R166-72 pubmed publisher
    ..CNS), Wistar rats were infused ICV with artificial CSF (aCSF), Na+ -rich (800 mmol/l) aCSF, aCSF plus the aldosterone synthase inhibitor FAD286 (100 microg x kg(-1) x day(-1)), or Na+ -rich aCSF plus FAD286...
  15. Lai L, Chen J, Hao C, Lin S, Gu Y. Aldosterone promotes fibronectin production through a Smad2-dependent TGF-beta1 pathway in mesangial cells. Biochem Biophys Res Commun. 2006;348:70-5 pubmed
    ..In cultured renal mesangial cells, aldosterone synthase (CYP11B2), mineralocorticoid receptor (MR), and 11beta-HSD2 mRNA expressions were detected by RT-PCR...
  16. Peters B, Teubner P, Clausmeyer S, Puschner T, Maser Gluth C, Wrede H, et al. StAR expression and the long-term aldosterone response to high-potassium diet in Wistar-Kyoto and spontaneously hypertensive rats. Am J Physiol Endocrinol Metab. 2007;292:E16-23 pubmed
    ..8 to 51 +/- 10.2 ng/100 ml, not significant). This increase was associated with an increase in Cyp11b2 mRNA levels in WKY (3-fold; P < 0.001) but not in SHR...
  17. Tanaka H, Watanabe K, Harima M, Thanikachalam P, Yamaguchi K, Tachikawa H, et al. [Effects of various diuretics on cardiac function in rats with heart failure]. Yakugaku Zasshi. 2009;129:871-9 pubmed
    ..myocardial function by echocardiography, cardiac fibrosis, myocyte diameter and cardiac aldosterone synthetase (CYP11B2) were evaluated...
  18. Safar M, Cattan V, Lacolley P, Nzietchueng R, Labat C, Lajemi M, et al. Aldosterone synthase gene polymorphism, stroke volume and age-related changes in aortic pulse wave velocity in subjects with hypertension. J Hypertens. 2005;23:1159-66 pubmed
    ..Whether the aldosterone synthase gene polymorphism (ASGP) CYP11B2 influences the age-related changes in blood pressure (BP) and arterial ..
  19. Veeraveedu P, Watanabe K, Ma M, Palaniyandi S, Yamaguchi K, Suzuki K, et al. Torasemide, a long-acting loop diuretic, reduces the progression of myocarditis to dilated cardiomyopathy. Eur J Pharmacol. 2008;581:121-31 pubmed
    ..myocyte size and the myocardial protein levels of transforming growth factor-beta1, collagen III, and aldosterone synthase were significantly decreased, and the sarcoplasmic reticulum Ca2+ ATPase2 protein level was significantly ..
  20. Fallo F, Pezzi V, Barzon L, Mulatero P, Veglio F, Sonino N, et al. Quantitative assessment of CYP11B1 and CYP11B2 expression in aldosterone-producing adenomas. Eur J Endocrinol. 2002;147:795-802 pubmed
    ..In order to specifically quantify CYP11B1, CYP11B2 (aldosterone synthase) and CYP17(17alpha-hydroxylase) mRNA levels, we developed a real-time RT-PCR assay and examined the ..
  21. Faroqui S, Sheriff S, Amlal H. Metabolic acidosis has dual effects on sodium handling by rat kidney. Am J Physiol Renal Physiol. 2006;291:F322-31 pubmed
    ..CMA enhanced serum aldosterone levels by 54% and increased the expression of aldosterone synthase in the adrenal gland by 134% after 5d of CMA...
  22. Okamoto M, Nonaka Y. Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]. J Steroid Biochem Mol Biol. 1992;41:415-9 pubmed
    The molecular features of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)] are discussed...
  23. Li S, Hu L, Wu Q, Huang P, Cao H. [The influence of suoquan capsule on the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats]. Zhong Yao Cai. 2011;34:80-3 pubmed
    ..of the kidney and diuresis by adenine,inspectd the contents of Cort, ALD in blood and the mRNA expression of CYP11B2 with ELISA and RT-PCR technology of the mice...
  24. Patel R, Lim D, Reddy D, Nagueh S, Lutucuta S, Sole M, et al. Variants of trophic factors and expression of cardiac hypertrophy in patients with hypertrophic cardiomyopathy. J Mol Cell Cardiol. 2000;32:2369-77 pubmed
    ..interleukin-6 (IL6), insulin-like growth factor-2 (IGF2), transforming growth factor- beta 1 (TGFB1), and aldosterone synthase (CYP11B2) genes, all previously implicated in cardiac hypertrophy, with the severity of LVH in patients ..
  25. Davies E, Holloway C, Ingram M, Inglis G, Friel E, Morrison C, et al. Aldosterone excretion rate and blood pressure in essential hypertension are related to polymorphic differences in the aldosterone synthase gene CYP11B2. Hypertension. 1999;33:703-7 pubmed
    ..Because essential hypertension is partly genetic, it is possible that altered control of aldosterone synthase gene expression or translation may be responsible...
  26. LeHoux J, Tremblay A. In vivo regulation of gene expression of enzymes controlling aldosterone synthesis in rat adrenal. J Steroid Biochem Mol Biol. 1992;43:837-46 pubmed publisher
    ..To further define the contribution of P450c11 and P450c18 (aldosterone synthase) in response to these differing intakes, we evaluated their mRNA levels using gene-specific oligonucleotide ..
  27. Nomura M, Morohashi K, Kirita S, Nonaka Y, Okamoto M, Nawata H, et al. Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene. J Biochem. 1993;113:144-52 pubmed
    ..Two of them corresponded to 11 beta-hydroxylase gene (CYP11B1) and aldosterone synthase gene (CYP11B2), respectively...
  28. Yilmaz M, Sari I, Bagci B, Gümüs E, Ozdemir O. Aldosterone Synthase CYP11B2 Gene Promoter Polymorphism in a Turkish Population With Chronic Kidney Disease. Iran J Kidney Dis. 2015;9:209-14 pubmed
    ..Many studies have indicated that aldosterone synthase CYP11B2 gene polymorphism (-344C>T) influences the aldosterone level, urinary aldosterone excretion, ..
  29. Ye P, Kenyon C, MacKenzie S, Nichol K, Seckl J, Fraser R, et al. Effects of ACTH, dexamethasone, and adrenalectomy on 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) gene expression in the rat central nervous system. J Endocrinol. 2008;196:305-11 pubmed publisher
    ..sensitive quantitative RT-PCR method for the measurement of CYP11B1 (11beta-hydroxylase) and CYP11B2 (aldosterone synthase) mRNAs, we previously demonstrated that CYP11B2 expression in the central nervous system (CNS) is subject ..
  30. Keavney B, Mayosi B, Gaukrodger N, Imrie H, Baker M, Fraser R, et al. Genetic variation at the locus encompassing 11-beta hydroxylase and aldosterone synthase accounts for heritability in cortisol precursor (11-deoxycortisol) urinary metabolite excretion. J Clin Endocrinol Metab. 2005;90:1072-7 pubmed
    Genetic variation in the gene encoding aldosterone synthase (CYP11B2) has previously been shown to be associated with hypertension and left ventricular hypertrophy...
  31. Lloyd MacGilp S, Torielli L, Bechtel S, Tripodi G, Gomez Sanchez C, Zagato L, et al. Mutations in aldosterone synthase gene of Milan hypertensive rats: phenotypic consequences. Am J Physiol Endocrinol Metab. 2002;282:E608-17 pubmed
    ..Genes encoding aldosterone synthase (CYP11B2) and 11beta-hydroxylase (CYP11B1) in MHS and MNS have been cloned and sequenced...
  32. Ortlepp J, Breithardt O, Ohme F, Hanrath P, Hoffmann R. Lack of association among five genetic polymorphisms of the renin-angiotensin system and cardiac hypertrophy in patients with aortic stenosis. Am Heart J. 2001;141:671-6 pubmed
    ..Five genetic polymorphisms of the RAAS (ACE, AGTR1, AGT, CMA, CYP11B2) were analyzed in all patients and the results of genetic analysis were correlated to severity of AS and LVH to ..
  33. Ko G, Kang Y, Lee M, Song H, Kim H, Cha D. Polymorphism of the aldosterone synthase gene is not associated with progression of diabetic nephropathy, but associated with hypertension in type 2 diabetic patients. Nephrology (Carlton). 2008;13:492-9 pubmed publisher
    ..system is beneficial in diabetic nephropathy, and aldosterone synthesis is regulated at the gene-encoding aldosterone synthase (CYP11B2)...
  34. Huang B, White R, Jeng A, Leenen F. Role of central nervous system aldosterone synthase and mineralocorticoid receptors in salt-induced hypertension in Dahl salt-sensitive rats. Am J Physiol Regul Integr Comp Physiol. 2009;296:R994-R1000 pubmed publisher
    ..of aldosterone locally produced in the brain, we evaluated the effects of chronic central blockade with the aldosterone synthase inhibitor FAD286 and the MR blocker spironolactone on changes in aldosterone and corticosterone content in ..
  35. Szekeres M, Turu G, Orient A, Szalai B, Süpeki K, Cserzo M, et al. Mechanisms of angiotensin II-mediated regulation of aldosterone synthase expression in H295R human adrenocortical and rat adrenal glomerulosa cells. Mol Cell Endocrinol. 2009;302:244-53 pubmed
    ..level of early transcription factors NR4A1 (NGFIB) and NR4A2 (Nurr1) genes, and the CYP11B2 gene encoding aldosterone synthase in H295R human adrenocortical tumor cells and in primary rat adrenal glomerulosa cells...
  36. Ohmine T, Miwa Y, Takahashi Yanaga F, Morimoto S, Maehara Y, Sasaguri T. The involvement of aldosterone in cyclic stretch-mediated activation of NADPH oxidase in vascular smooth muscle cells. Hypertens Res. 2009;32:690-9 pubmed publisher
    ..CS strength-dependently enhanced NADPH oxidase activity. CS induced cytochrome P450 aldosterone synthase (CYP11B2) and increased aldosterone synthesis but did not influence the levels of 11beta-hydroxysteroid ..
  37. Somekawa S, Imagawa K, Naya N, Takemoto Y, Onoue K, Okayama S, et al. Regulation of aldosterone and cortisol production by the transcriptional repressor neuron restrictive silencer factor. Endocrinology. 2009;150:3110-7 pubmed publisher
    b>Aldosterone synthase (CYP11B2) and 11 beta-hydroxylase (CYP11B1) regulate aldosterone and cortisol production, respectively. The expression of these enzymes is promoted by calcium influx through Cav3.2, a T-type calcium channel...
  38. Fiebeler A, Nussberger J, Shagdarsuren E, Rong S, Hilfenhaus G, Al Saadi N, et al. Aldosterone synthase inhibitor ameliorates angiotensin II-induced organ damage. Circulation. 2005;111:3087-94 pubmed
    ..Aldosterone concentrations depend on the activity of aldosterone synthase (CYP11B2)...
  39. Mukai K, Imai M, Shimada H, Ishimura Y. Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses. J Biol Chem. 1993;268:9130-7 pubmed
    ..were confined to these four genes; among them, CYP11B1 and -B2 encoded steroid 11 beta-hydroxylase and aldosterone synthase, respectively, while CYP11B3 was a gene highly homologous to CYP11B1 without a known expression product...
  40. Taira M, Toba H, Murakami M, Iga I, Serizawa R, Murata S, et al. Spironolactone exhibits direct renoprotective effects and inhibits renal renin-angiotensin-aldosterone system in diabetic rats. Eur J Pharmacol. 2008;589:264-71 pubmed publisher
    Aldosterone itself has been reported to participate in mediating renal injury, and it was confirmed that the aldosterone synthase CYP11B2 gene, protein, and aldosterone production are locally present in the kidney...
  41. Kawainoto T, Mitsuuchi Y, Ohnishi T, Ichikawa Y, Yokoyama Y, Sumimoto H, et al. Cloning and expression of a cDNA for human cytochrome P-450aldo as related to primary aldosteronism. Biochem Biophys Res Commun. 1990;173:309-16 pubmed
    A cDNA clone encoding human aldosterone synthase cytochrome P-450 (P-450aldo) has been isolated from a cDNA library derived from human adrenal tumor of a patient suffering from primary aldosteronism...
  42. Garnier A, Bendall J, Fuchs S, Escoubet B, Rochais F, Hoerter J, et al. Cardiac specific increase in aldosterone production induces coronary dysfunction in aldosterone synthase-transgenic mice. Circulation. 2004;110:1819-25 pubmed
    ..Transgenic mice (TG) that overexpress the terminal enzyme of aldosterone biosynthesis, aldosterone synthase (AS), in heart have been raised by gene targeting with the alpha-myosin heavy chain promoter...
  43. Ito T, Yoshimura M, Nakamura S, Nakayama M, Shimasaki Y, Harada E, et al. Inhibitory effect of natriuretic peptides on aldosterone synthase gene expression in cultured neonatal rat cardiocytes. Circulation. 2003;107:807-10 pubmed
    ..solely in adrenal cortex, we have recently showed that aldosterone is also produced in and the expression of CYP11B2 mRNA was induced in the failing or hypertensive human ventricle...
  44. Curnow K, Slutsker L, Vitek J, Cole T, Speiser P, New M, et al. Mutations in the CYP11B1 gene causing congenital adrenal hyperplasia and hypertension cluster in exons 6, 7, and 8. Proc Natl Acad Sci U S A. 1993;90:4552-6 pubmed
    ..In principle, deletions of CYP11B1 could be generated by unequal crossing-over between CYP11B1 and the adjacent CYP11B2 gene, but no such deletions were found among the deficiency alleles in this study...