CTP

Summary

Gene Symbol: CTP
Description: phosphate cytidylyltransferase 1, choline, alpha
Alias: CCT-alpha, CTP, choline-phosphate cytidylyltransferase A, CCT A, CT A, CTP:phosphocholine cytidylyl transferase, CTP:phosphocholine cytidylyltransferase A, CTP:phosphocholine cytidylyltransferase alpha, choline phosphate cytidylyltransferase 1 alpha, phosphate cytidylyltransferase 1, choline, alpha isoform, phosphorylcholine transferase A
Species: rat
Products:     CTP

Top Publications

  1. Bakovic M, Waite K, Vance D. Oncogenic Ha-Ras transformation modulates the transcription of the CTP:phosphocholine cytidylyltransferase alpha gene via p42/44MAPK and transcription factor Sp3. J Biol Chem. 2003;278:14753-61 pubmed
    We have shown previously that expression of the murine CTP:phosphocholine cytidylyltransferase (CT) alpha gene is regulated during cell proliferation (Golfman, L. S., Bakovic, M., and Vance, D. E. (2001) J. Biol. Chem. 276, 43688-43692)...
  2. Gehrig K, Ridgway N. CTP:phosphocholine cytidylyltransferase ? (CCT?) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis. Biochim Biophys Acta. 2011;1811:377-85 pubmed publisher
    b>CTP:phosphocholine cytidylyltransferase ? (CCT?) is a nuclear enzyme that catalyzes the rate-limiting step in the CDP-choline pathway for phosphatidylcholine (PC) synthesis...
  3. Ding Z, Taneva S, Huang H, Campbell S, Semenec L, Chen N, et al. A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions. J Biol Chem. 2012;287:38980-91 pubmed publisher
    b>CTP:phosphocholine cytidylyltransferase (CCT), an amphitropic enzyme that regulates phosphatidylcholine synthesis, is composed of a catalytic head domain and a regulatory tail...
  4. Mallampalli R, Ryan A, Carroll J, Osborne T, Thomas C. Lipid deprivation increases surfactant phosphatidylcholine synthesis via a sterol-sensitive regulatory element within the CTP:phosphocholine cytidylyltransferase promoter. Biochem J. 2002;362:81-8 pubmed
    ..phosphatidylcholine (DSPtdCho) synthesis compared with mice fed a standard diet by increasing expression of CTP:phosphocholine cytidylyltransferase (CCT), the rate-limiting enzyme for DSPtdCho synthesis...
  5. Helmink B, Braker J, Kent C, Friesen J. Identification of lysine 122 and arginine 196 as important functional residues of rat CTP:phosphocholine cytidylyltransferase alpha. Biochemistry. 2003;42:5043-51 pubmed
    b>CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) contains a central region that functions as a catalytic domain, converting phosphocholine and cytidine 5'-triphosphate (CTP) to CDP-choline for the subsequent synthesis of ..
  6. Xie M, Smith J, Ding Z, Zhang D, Cornell R. Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase. J Biol Chem. 2004;279:28817-25 pubmed
    b>CTP:phosphocholine cytidylyltransferase (CCT), a key enzyme that controls phosphatidylcholine synthesis, is regulated by reversible interactions with membranes containing anionic lipids. Previous work demonstrated that CCT is a homodimer...
  7. MacDonald J, Kent C. Identification of phosphorylation sites in rat liver CTP: phosphocholine cytidylyltransferase. J Biol Chem. 1994;269:10529-37 pubmed
    b>CTP:phosphocholine cytidylyltransferase (CT) is an important regulatory enzyme in phosphatidylcholine biosynthesis...
  8. Dunne S, Cornell R, Johnson J, Glover N, Tracey A. Structure of the membrane binding domain of CTP:phosphocholine cytidylyltransferase. Biochemistry. 1996;35:11975-84 pubmed
    It has been proposed that the domain of the regulatory enzyme, CTP:phosphocholine cytidylyltransferase, which mediates reversible binding of the enzyme to membranes, is an amphipathic alpha-helix of approximately 60 amino acid residues ..
  9. Ridsdale R, Tseu I, Wang J, Post M. CTP:phosphocholine cytidylyltransferase alpha is a cytosolic protein in pulmonary epithelial cells and tissues. J Biol Chem. 2001;276:49148-55 pubmed
    ..The localization of CCT alpha in lung epithelial cells and, of greater consequence, lung tissues are yet unknown...

More Information

Publications25

  1. Johnson J, Xie M, Singh L, Edge R, Cornell R. Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranes. J Biol Chem. 2003;278:514-22 pubmed
    ..Thus anionic lipids, working via surface-localized pH effects, can promote membrane binding by modifying protein charge and hydrophobicity, and this novel mechanism contributes to the membrane selectivity of CT in vivo. ..
  2. Ridsdale R, Post M. Surfactant lipid synthesis and lamellar body formation in glycogen-laden type II cells. Am J Physiol Lung Cell Mol Physiol. 2004;287:L743-51 pubmed
    ..we determined the subcellular localization of two key enzymes for PC biosynthesis, fatty acid synthase (FAS) and CTP:phosphocholine cytidylyltransferase-alpha (CCT-alpha), and compared their localization with that of surfactant ..
  3. Favale N, Pescio L, Santacreu B, Marquez M, Sterin Speziale N. Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells. Biochim Biophys Acta. 2016;1861:513-23 pubmed publisher
    ..PC biosynthetic pathway is generally controlled by CTP:phosphocholine cytidylyltransferase (CCT), which is considered the rate-limiting enzyme...
  4. Kalmar G, Kay R, Lachance A, Aebersold R, Cornell R. Cloning and expression of rat liver CTP: phosphocholine cytidylyltransferase: an amphipathic protein that controls phosphatidylcholine synthesis. Proc Natl Acad Sci U S A. 1990;87:6029-33 pubmed
    b>CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) is a key regulatory enzyme in the synthesis of phosphatidylcholine in higher eukaryotes...
  5. Bogan M, Agnes G, Pio F, Cornell R. Interdomain and membrane interactions of CTP:phosphocholine cytidylyltransferase revealed via limited proteolysis and mass spectrometry. J Biol Chem. 2005;280:19613-24 pubmed
    b>CTP:phosphocholine cytidylyltransferase (CCT) is a multi-domain enzyme that regulates phosphatidylcholine synthesis...
  6. Gehrig K, Lagace T, Ridgway N. Oxysterol activation of phosphatidylcholine synthesis involves CTP:phosphocholine cytidylyltransferase alpha translocation to the nuclear envelope. Biochem J. 2009;418:209-17 pubmed publisher
    ..and 27OH (27-hydroxycholesterol) increased PtdCho synthesis in CHO cells as a result of CCTalpha (CTP:phosphocholine cytidylyltransferase alpha) translocation and activation at the NE (nuclear envelope)...
  7. Guan C, Zhou F, Luo Z, Zhang C, Qin X. [Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003;19:57-9 pubmed
    To investigate the influence and mechanisms of 17beta-estradiol on the CTP: phosphorylcholine cytidylyltransferase (CCT) activity from cultured lung explants without serum...
  8. Ridsdale R, Tseu I, Wang J, Post M. Functions of membrane binding domain of CTP:phosphocholine cytidylyltransferase in alveolar type II cells. Am J Respir Cell Mol Biol. 2010;43:74-87 pubmed publisher
    b>CTP:phosphocholine cytidylyltransferase (CCTalpha) plays a key role in the biosynthesis of surfactant phosphatidylcholine...
  9. Wang L, Magdaleno S, Tabas I, Jackowski S. Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase alpha gene (Pcyt1a). Mol Cell Biol. 2005;25:3357-63 pubmed
    b>CTP:phosphocholine cytidylyltransferase (CCT) catalyzes a rate-controlling step in the biosynthesis of phosphatidylcholine (PtdCho). Multiple CCT isoforms, CCTalpha, CCTbeta2, and CCTbeta3, are encoded by two genes, Pcyt1a and Pcyt1b...
  10. Chen B, Mallampalli R. Calmodulin binds and stabilizes the regulatory enzyme, CTP: phosphocholine cytidylyltransferase. J Biol Chem. 2007;282:33494-506 pubmed
    b>CTP:phosphocholine cytidylyltransferase (CCTalpha) is a proteolytically sensitive enzyme essential for production of phosphatidylcholine, the major phospholipid of animal cell membranes...
  11. Chen B, Mallampalli R. Masking of a nuclear signal motif by monoubiquitination leads to mislocalization and degradation of the regulatory enzyme cytidylyltransferase. Mol Cell Biol. 2009;29:3062-75 pubmed publisher
    ..These results unravel a unique molecular mechanism whereby monoubiquitination governs the trafficking and life span of a critical regulatory enzyme in vivo. ..
  12. Lee J, Johnson J, Ding Z, Paetzel M, Cornell R. Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold. J Biol Chem. 2009;284:33535-48 pubmed publisher
    b>CTP:phosphocholine cytidylyltransferase (CCT) is the key regulatory enzyme in the synthesis of phosphatidylcholine, the most abundant phospholipid in eukaryotic cell membranes...
  13. Yang J, Wang J, Tseu I, Kuliszewski M, Lee W, Post M. Identification of an 11-residue portion of CTP-phosphocholine cytidylyltransferase that is required for enzyme-membrane interactions. Biochem J. 1997;325 ( Pt 1):29-38 pubmed
    b>CTP-phosphocholine cytidylyltransferase (CT) is a key regulatory enzyme in the biosynthesis of phosphatidylcholine (PC) in many cells. Enzyme-membrane interactions appear to play an important role in CT activation...
  14. Arsenault D, Yoo B, Rosen K, Ridgway N. ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase ? contributes to malignant transformation of intestinal epithelial cells. J Biol Chem. 2013;288:633-43 pubmed publisher
    ..As the rate-limiting enzyme in the CDP-choline pathway for PC synthesis, CTP:phosphocholine cytidylyltransferase ? (CCT?) is implicated in the provision of membranes and bioactive lipids ..
  15. Lee J, Taneva S, Holland B, Tieleman D, Cornell R. Structural basis for autoinhibition of CTP:phosphocholine cytidylyltransferase (CCT), the regulatory enzyme in phosphatidylcholine synthesis, by its membrane-binding amphipathic helix. J Biol Chem. 2014;289:1742-55 pubmed publisher
    b>CTP:phosphocholine cytidylyltransferase (CCT) interconverts between an inactive soluble and active membrane-bound form in response to changes in membrane lipid composition...
  16. MacDonald J, Kent C. Baculovirus-mediated expression of rat liver CTP:phosphocholine cytidylyltransferase. Protein Expr Purif. 1993;4:1-7 pubmed
    We describe herein the expression and purification of milligram quantities of rat liver CTP:phosphocholine cytidylyltransferase in recombinant baculovirus-infected insect cells...