Gene Symbol: Cpsf6
Description: cleavage and polyadenylation specific factor 6
Alias: cleavage and polyadenylation specificity factor subunit 6, cleavage and polyadenylation specific factor 6, 68kDa
Brown K, Gilmartin G. A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im. Mol Cell. 2003;12:1467-76 pubmed
..These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor. ..
Gruber A, Martin G, Keller W, Zavolan M. Cleavage factor Im is a key regulator of 3' UTR length. RNA Biol. 2012;9:1405-12 pubmed publisher
..More specifically, we demonstrate that the loss-of-function of CF Im 68 and CF Im 25 but not of CF Im 59 leads to a transcriptome-wide increase in the use of proximal polyadenylation sites in HEK293 cells. ..
Chan S, Choi E, Shi Y. Pre-mRNA 3'-end processing complex assembly and function. Wiley Interdiscip Rev RNA. 2011;2:321-35 pubmed publisher
..In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges. ..
Rüegsegger U, Blank D, Keller W. Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Mol Cell. 1998;1:243-53 pubmed
Dettwiler S, Aringhieri C, Cardinale S, Keller W, Barabino S. Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization. J Biol Chem. 2004;279:35788-97 pubmed
..However, CF I(m)68 does not concentrate in splicing speckles but in foci that partially colocalize with paraspeckles, a subnuclear component in which other proteins involved in transcriptional control and RNA processing have been found. ..
Ruepp M, Aringhieri C, Vivarelli S, Cardinale S, Paro S, Schumperli D, et al
. Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export. Mol Biol Cell. 2009;20:5211-23 pubmed publisher
..These results reveal a novel function for the pre-mRNA 3' end processing factor CF I(m)68 in mRNA export. ..
Kim S, Yamamoto J, Chen Y, Aida M, Wada T, Handa H, et al
. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. Genes Cells. 2010;15:1003-13 pubmed publisher
..Thus, this study establishes that CFIm not only plays a general role in 3'-end processing but also plays a regulatory role in poly(A) site selection. ..
Yang Q, Coseno M, Gilmartin G, DOUBLIE S. Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA complex provides an insight into poly(A) site recognition and RNA looping. Structure. 2011;19:368-77 pubmed publisher
..The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection. ..
Gruber A, Martin G, Keller W, Zavolan M. Means to an end: mechanisms of alternative polyadenylation of messenger RNA precursors. Wiley Interdiscip Rev RNA. 2014;5:183-96 pubmed publisher
..Here, we review these approaches as well as the insights into the mechanisms of polyadenylation that emerged from genome-wide studies of polyadenylation across a range of cell types and states. ..