Gene Symbol: Cpb1
Description: carboxypeptidase B1
Alias: Cpb, carboxypeptidase B, carboxypeptidase B1 (tissue), pancreatic carboxypeptidase B1
Species: rat
Products:     Cpb1

Top Publications

  1. Dubay C, Vincent M, Samani N, Hilbert P, Kaiser M, Beressi J, et al. Genetic determinants of diastolic and pulse pressure map to different loci in Lyon hypertensive rats. Nat Genet. 1993;3:354-7 pubmed
    ..and a microsatellite marker of the renin gene (REN) on rat chromosome 13, and between pulse pressure and the carboxypeptidase B gene (CPB) on chromosome 2...
  2. Clark J, Jeffs B, Davidson A, Lee W, Anderson N, Bihoreau M, et al. Quantitative trait loci in genetically hypertensive rats. Possible sex specificity. Hypertension. 1996;28:898-906 pubmed
    ..1). The sex specificity of blood pressure quantitative trait loci will be important in designing congenic strains and substrains for fine genetic mapping and for identifying genes that regulate blood pressure. ..
  3. Hadkar V, Sangsree S, Vogel S, Brovkovych V, Skidgel R. Carboxypeptidase-mediated enhancement of nitric oxide production in rat lungs and microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2004;287:L35-45 pubmed
    ..These studies reveal a novel mechanism by which the Arg supply for NO production in inflammatory conditions may be maintained. ..
  4. Xiao Yan Z, Su Xia L, Qin Sheng Y. The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B. Protein Pept Lett. 2005;12:671-6 pubmed
    ..Subsequently, the renatured pCPB was digested by trypsin. Recombinant active CPB was obtained by passing through DEAE-FF ion exchange and Sephadex-G100 chromatographic column...
  5. Oliveira E, Souza L, Sivieri D, Bispo da Silva L, Pereira H, Costa Neto C, et al. Carboxypeptidase B and other kininases of the rat coronary and mesenteric arterial bed perfusates. Am J Physiol Heart Circ Physiol. 2007;293:H3550-7 pubmed
    ..potato carboxypeptidase inhibitor-Sepharose column and shown to be structurally identical to rat pancreatic carboxypeptidase B (CPB)...
  6. Kodama H, Shimojo N, Suzuki K. Distribution of manganese in rat pancreas and identification of its primary binding protein as pro-carboxypeptidase B. Biochem J. 1991;278 ( Pt 3):857-62 pubmed
    ..On the basis of enzymic and chemical characterization of the protein, it was identified as a zymogen of carboxypeptidase B (pro-carboxypeptidase B, pro-CPB). The elution profiles of the protein by h.p.l.c.-i.c.p...
  7. Clauser E, Gardell S, Craik C, MacDonald R, Rutter W. Structural characterization of the rat carboxypeptidase A1 and B genes. Comparative analysis of the rat carboxypeptidase gene family. J Biol Chem. 1988;263:17837-45 pubmed
    Nucleotide sequencing of a rat carboxypeptidase B (CPB) cDNA and direct sequencing of the CPB mRNA via primer extension on pancreatic polyadenylated RNA has yielded the complete amino acid sequence of rat CPB...
  8. Laes J, Ravoet M, Quan X, Van Vooren P, Szpirer J, Szpirer C. Improved radiation hybrid map of rat chromosome 2: colocalization of the genes encoding corticotropin-releasing hormone and IL6-receptor with quantitative trait loci regulating the inflammatory response. Cytogenet Cell Genet. 2001;92:130-3 pubmed
    ..Our data show that they reside within regions that were genetically defined as QTLs controlling the inflammatory response. These genes are thus both functional and positional candidates. ..
  9. Wang D, Miao L, Chen H, Li Y, Chen H, Fang H. [Expression, purification and characterization of rat procarboxypeptidase B in Pichia pastoris]. Sheng Wu Gong Cheng Xue Bao. 2007;23:61-6 pubmed
    b>Carboxypeptidase B is a metalloenzyme, which is widely used for commercial and research purposes. Commercially available CPB purified from porcine or bovine pancreas is very expensive, and is not totally free from other proteases...

More Information


  1. Li S, Zhang L, Wu Q, Xin A, Zhao J, Fan L. Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B. Enzyme Microb Technol. 2011;49:139-45 pubmed publisher
    ..The Cys383 mutations changed some of the properties of rat carboxypeptidase B. Mutants C383G, C383A had higher k(cat)/K(m) values which indicated increased catalytic abilities...