Gene Symbol: Cnp
Description: 2',3'-cyclic nucleotide 3' phosphodiesterase
Alias: CNPF, CNPI, CNPII, Cnp1, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNPase
Species: rat
Products:     Cnp

Top Publications

  1. Bifulco M, Laezza C, Stingo S, Wolff J. 2',3'-Cyclic nucleotide 3'-phosphodiesterase: a membrane-bound, microtubule-associated protein and membrane anchor for tubulin. Proc Natl Acad Sci U S A. 2002;99:1807-12 pubmed
    2',3'-Cyclic nucleotide-3'-phosphodiesterase (CNP) is firmly associated with tubulin from brain tissue and FRTL-5 thyroid cells as demonstrated by copolymerization with microtubules through several warm/cold cycles, the presence of CNP ..
  2. Gravel M, Robert F, Kottis V, Gallouzi I, Pelletier J, Braun P. 2',3'-Cyclic nucleotide 3'-phosphodiesterase: a novel RNA-binding protein that inhibits protein synthesis. J Neurosci Res. 2009;87:1069-79 pubmed publisher
    ..We also demonstrate that CNP1 can bridge an association between tubulin and RNA...
  3. Peirce T, Bray N, Williams N, Norton N, Moskvina V, Preece A, et al. Convergent evidence for 2',3'-cyclic nucleotide 3'-phosphodiesterase as a possible susceptibility gene for schizophrenia. Arch Gen Psychiatry. 2006;63:18-24 pubmed
    Convergent data make 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) a candidate gene for schizophrenia. Reduced expression has been reported in the schizophrenic brain...
  4. Lappe Siefke C, Goebbels S, Gravel M, Nicksch E, Lee J, Braun P, et al. Disruption of Cnp1 uncouples oligodendroglial functions in axonal support and myelination. Nat Genet. 2003;33:366-74 pubmed
    ..But long-term interactions between axons and their myelin sheaths are poorly understood. Here we show that Cnp1, which encodes 2',3'-cyclic nucleotide phosphodiesterase in oligodendrocytes, is essential for axonal survival but ..
  5. Lee J, Gravel M, Zhang R, Thibault P, Braun P. Process outgrowth in oligodendrocytes is mediated by CNP, a novel microtubule assembly myelin protein. J Cell Biol. 2005;170:661-73 pubmed
    ..Several lines of evidence suggest a role for 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in mediating process formation in OLs. In this study, we report that tubulin is a major CNP-interacting protein...
  6. Kozlov G, Lee J, Elias D, Gravel M, Gutierrez P, Ekiel I, et al. Structural evidence that brain cyclic nucleotide phosphodiesterase is a member of the 2H phosphodiesterase superfamily. J Biol Chem. 2003;278:46021-8 pubmed
    2',3'-Cyclic-nucleotide 3'-phosphodiesterase (CNP) is an enzyme abundantly present in the central nervous system of mammals and some vertebrates...
  7. Gobert R, Joubert L, Curchod M, Salvat C, Foucault I, Jorand Lebrun C, et al. Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits. Mol Cell Biol. 2009;29:1538-53 pubmed publisher
    ..Among these repressors were CNP, a well-known myelin constituent, and three phosphatases, each known to negatively control mitogen-activated ..
  8. Jackson E, Gillespie D, Mi Z, Cheng D, Bansal R, Janesko Feldman K, et al. Role of 2',3'-cyclic nucleotide 3'-phosphodiesterase in the renal 2',3'-cAMP-adenosine pathway. Am J Physiol Renal Physiol. 2014;307:F14-24 pubmed publisher
    ..this "2',3'-cAMP-adenosine pathway" are unknown, we examined whether 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) participates in the renal metabolism of 2',3'-cAMP...
  9. Dabrowska Bouta B, Sulkowski G, Struzynska L, Rafałowska U. CNPase activity in myelin from adult rat brains after prolonged lead exposure in vivo. Chem Biol Interact. 2004;150:171-8 pubmed
    ..Both, the protein content and the activity of the myelin-specific enzyme CNPase (2',3'-cyclic nucleotide 3-phosphodiesterase), were lowered...

More Information


  1. Huang Z, Liu J, Cheung P, Chen C. Long-term cognitive impairment and myelination deficiency in a rat model of perinatal hypoxic-ischemic brain injury. Brain Res. 2009;1301:100-9 pubmed publisher
    ..There were significant decreases in myelin basic protein (MBP)and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-labeled oligodendrocytes with glial fibrillary acidic protein (GFAP)-labeled glial scarring in the ipsilateral ..
  2. Chen L, Lu W, Yang Z, Yang S, Li C, Shi X, et al. Age-related changes of the oligodendrocytes in rat subcortical white matter. Anat Rec (Hoboken). 2011;294:487-93 pubmed publisher subcortical white matter were labeled with anti-2',3'-cyclic nucleotide 3'-phosphodiesterase antibody (anti-CNPase antibody, a specific marker of oligodendrocytes)...
  3. Gomes S, Carvalho S, Santiago M, Lopez L, Barradas P, Cavalcante L. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in the developing olfactory bulb and subventricular zone rostral extension. J Neurosci Res. 2003;73:471-80 pubmed
    ..We studied the expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), a protein related to axonal ensheathment by myelinating cells...
  4. Kanno T, Sasaki S, Yamada N, Kawasako K, Tsuchitani M. Hexachlorophene and cuprizone induce the spongy change of the developing rat brain by different mechanisms: the role of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase). J Vet Med Sci. 2012;74:837-43 pubmed
    ..On day 12, cerebral cortex oligodendroglia were stained strongly by anti-CNPase. Other rats were fed for 8 days with powdered chow containing 1% (w/w) CPZ, which was then withdrawn for 16 days...
  5. Zhao Y, Shi X, Qiu X, Zhang L, Lu W, Yang S, et al. Enriched environment increases the total number of CNPase positive cells in the corpus callosum of middle-aged rats. Acta Neurobiol Exp (Wars). 2011;71:322-30 pubmed, the effect of enriched environment on the total number of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) positive cells in middle-aged rat corpus callosum was investigated by means of immunohistochemical techniques and ..
  6. Hagemeyer N, Goebbels S, Papiol S, Kastner A, Hofer S, Begemann M, et al. A myelin gene causative of a catatonia-depression syndrome upon aging. EMBO Mol Med. 2012;4:528-39 pubmed publisher
    ..However, cause and effect have remained difficult to distinguish. CNP (2',3'-cyclic nucleotide 3'-phosphodiesterase) is among the oligodendrocyte/myelin-associated genes most robustly ..
  7. Georgieva L, Moskvina V, Peirce T, Norton N, Bray N, Jones L, et al. Convergent evidence that oligodendrocyte lineage transcription factor 2 (OLIG2) and interacting genes influence susceptibility to schizophrenia. Proc Natl Acad Sci U S A. 2006;103:12469-74 pubmed
    ..of relevance to oligodendrocyte function for which we have reported evidence for association with schizophrenia: CNP, NRG1, and ERBB4. We found interaction effects on disease risk between OLIG2 and CNP (minimum P = 0...
  8. Parenti R, Cicirata F, Zappala A, Catania A, La Delia F, Cicirata V, et al. Dynamic expression of Cx47 in mouse brain development and in the cuprizone model of myelin plasticity. Glia. 2010;58:1594-609 pubmed publisher
    ..Taken as a whole the evidence suggests that Cx47 play a key role in myelination. ..
  9. Schwer B, Aronova A, Ramirez A, Braun P, Shuman S. Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. RNA. 2008;14:204-10 pubmed
    ..Here we report that a mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can perform the essential 3' end-healing steps of tRNA splicing in yeast and thereby complement growth of strains ..
  10. Ulrich R, Seeliger F, Kreutzer M, Germann P, Baumgartner W. Limited remyelination in Theiler's murine encephalomyelitis due to insufficient oligodendroglial differentiation of nerve/glial antigen 2 (NG2)-positive putative oligodendroglial progenitor cells. Neuropathol Appl Neurobiol. 2008;34:603-20 pubmed publisher
    ..In contrast, intralesional 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive oligodendrocytes were decreased between 56 and 196 dpi...
  11. Krestinina O, Azarashvili T, Baburina Y, Galvita A, Grachev D, Stricker R, et al. In aging, the vulnerability of rat brain mitochondria is enhanced due to reduced level of 2',3'-cyclic nucleotide-3'-phosphodiesterase (CNP) and subsequently increased permeability transition in brain mitochondria in old animals. Neurochem Int. 2015;80:41-50 pubmed publisher regulators of mPTP function in brain mitochondria, the enzyme 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and its substrates 2', 3'-cAMP and 2', 3'-cNADP, and the neuronal protein p42(IP4)...
  12. Lewohl J, Wixey J, Harper C, Dodd P. Expression of MBP, PLP, MAG, CNP, and GFAP in the Human Alcoholic Brain. Alcohol Clin Exp Res. 2005;29:1698-705 pubmed
    ..Future studies comparing cases with alcoholic and nonalcoholic cirrhosis of the liver disease are required to elucidate this further. ..
  13. Kooi E, van Horssen J, Witte M, Amor S, Bø L, Dijkstra C, et al. Abundant extracellular myelin in the meninges of patients with multiple sclerosis. Neuropathol Appl Neurobiol. 2009;35:283-95 pubmed publisher
    ..basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG) and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)]...
  14. Wu C, Lu J, Cao Q, Guo C, Gao Q, Ling E. Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase in the amoeboid microglial cells in the developing rat brain. Neuroscience. 2006;142:333-41 pubmed
    Expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in amoeboid microglial cells (AMC) in developing rat brain from prenatal day 18 (E18) to postnatal day 10 (P10) was demonstrated by immunohistochemistry/..
  15. Connor J, Ponnuru P, Lee B, Podskalny G, Alam S, Allen R, et al. Postmortem and imaging based analyses reveal CNS decreased myelination in restless legs syndrome. Sleep Med. 2011;12:614-9 pubmed publisher
    ..Iron deficiency is associated with hypomyelination in brains of animals. Therefore we hypothesized that a myelin deficit should be present in the brains of patients with RLS...
  16. Degano A, Roth G. Passive transfer of experimental autoimmune encephalomyelitis in Wistar rats: dissociation of clinical symptoms and biochemical alterations. J Neurosci Res. 2000;59:283-90 pubmed
  17. Cho S, Jung J, Jin I, Moon I. 2', 3'-cyclic nucleotide 3'-phosphodiesterase is expressed in dissociated rat cerebellar cells and included in the postsynaptic density fraction. Mol Cells. 2003;16:128-35 pubmed
    ..Immunoblot analysis indicated that both CNP1 and CNP2 isoforms are present in the CBL-PSD fraction, whereas there is little CNP2 in the forebrain (FB)-PSD ..
  18. Yao D, Liu X, Hudson L, Webster H. Insulin-like growth factor I treatment reduces demyelination and up-regulates gene expression of myelin-related proteins in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci U S A. 1995;92:6190-4 pubmed
    ..These IGF-I actions may also benefit patients with multiple sclerosis. ..
  19. Roberson R, Woodard J, Toso L, Abebe D, Poggi S, Spong C. Postnatal inflammatory rat model for cerebral palsy: too different from humans. Am J Obstet Gynecol. 2006;195:1038-44 pubmed
    ..White matter damage was assessed with immunohistochemical Pre-OL markers (CNP, PLP). Statistical analysis included Mann-Whitney U and analysis of variance...
  20. Chen S, Wang J, Irwin R, Yao J, Liu L, Brinton R. Allopregnanolone promotes regeneration and reduces β-amyloid burden in a preclinical model of Alzheimer's disease. PLoS ONE. 2011;6:e24293 pubmed publisher
    ..APα significantly reduced microglia activation as indicated by reduced expression of OX42 while increasing CNPase, an oligodendrocyte myelin marker...
  21. Kanno T, Kurotaki T, Yamada N, Yamashita K, Wako Y, Tsuchitani M. Activity of 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in spinal cord with spongy change induced by a single oral dose of aniline in rats. Toxicol Pathol. 2010;38:359-65 pubmed publisher
    ..In all rats, immunohistochemical staining by anti-2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) occurred along the cytoplasmic boundaries of the normal oligodendroglia...
  22. Svetlov S, Prima V, Kirk D, Gutierrez H, Curley K, Hayes R, et al. Morphologic and biochemical characterization of brain injury in a model of controlled blast overpressure exposure. J Trauma. 2010;69:795-804 pubmed publisher
    ..GFAP and 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), markers of astroglia and oligodendroglia, accumulated substantially in the hippocampus 24 hours after blast and ..
  23. Padhi B, Rosales M, Pelletier G. Perinatal methylmercury exposure perturbs the expression of Plp1 and Cnp splice variants in cerebellum of rat pups. Neurotoxicology. 2015;48:223-30 pubmed publisher
    ..The Proteolipid Protein 1 (Plp1), Myelin Basic Protein (Mbp) and 2',3'-Cyclic Nucleotide 3'Phosphodiesterase (Cnp) genes expressed in oligodendrocytes and involved in myelination processes can be useful biomarkers of potential ..
  24. Azarashvili T, Krestinina O, Galvita A, Grachev D, Baburina Y, Stricker R, et al. Ca2+-dependent permeability transition regulation in rat brain mitochondria by 2',3'-cyclic nucleotides and 2',3'-cyclic nucleotide 3'-phosphodiesterase. Am J Physiol Cell Physiol. 2009;296:C1428-39 pubmed publisher
    Recent evidence indicates that 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), a marker enzyme of myelin and oligodendrocytes, is also present in neural and nonneural mitochondria...
  25. Zhang B, Cao Q, Guo A, Chu H, Chan Y, Buschdorf J, et al. Juxtanodin: an oligodendroglial protein that promotes cellular arborization and 2',3'-cyclic nucleotide-3'-phosphodiesterase trafficking. Proc Natl Acad Sci U S A. 2005;102:11527-32 pubmed
    ..At the cellular level, JN in the rat CNS colocalized with 2',3'-cyclic nucleotide-3'-phosphodiesterase (CNPase), a cytoskeleton-related oligodendroglial protein...
  26. Yamaguchi Y, Miyagi Y, Baba H. Two-dimensional electrophoresis with cationic detergents: a powerful tool for the proteomic analysis of myelin proteins. Part 2: analytical aspects. J Neurosci Res. 2008;86:766-75 pubmed
  27. Lin K, Lai S. Induction of 2',3'-cyclic nucleotide 3'-phosphodiesterase in demyelination of BALB/c mice caused by Angiostrongylus cantonensis. J Comp Pathol. 2009;141:248-53 pubmed publisher
    ..The biological role of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) in myelin-related disease requires investigation...
  28. Lin S, Huang Y, Zhang L, Heng M, Ptacek L, Fu Y. MicroRNA-23a promotes myelination in the central nervous system. Proc Natl Acad Sci U S A. 2013;110:17468-73 pubmed publisher
    ..The data presented here imply a unique role for miR-23a in the coordination of proteins and noncoding RNAs in generating and maintaining healthy myelin. ..
  29. Gravel M, DeAngelis D, Braun P. Molecular cloning and characterization of rat brain 2',3'-cyclic nucleotide 3'-phosphodiesterase isoform 2. J Neurosci Res. 1994;38:243-7 pubmed
    ..Errors in the published sequence of rat CNP1 have now been corrected...
  30. Sajad M, Zargan J, Chawla R, Umar S, Khan H. Upregulation of CSPG3 accompanies neuronal progenitor proliferation and migration in EAE. J Mol Neurosci. 2011;43:531-40 pubmed publisher
    ..polymorphonuclear cell infiltration and loss of myelin basic protein expression along with steep decrease of CNPase. Periventricular white matter (PVWM) and cortex presented with astrogliosis evidenced by increased Glial ..
  31. Lee J, Park S, Shin Y, Kim C, Lee W, Hong K. Concurrent administration of cilostazol with donepezil effectively improves cognitive dysfunction with increased neuroprotection after chronic cerebral hypoperfusion in rats. Brain Res. 2007;1185:246-55 pubmed a large increase in glial fibrillary acidic protein (GFAP) immunoreactivity with significantly decreased CNPase-positive oligodendrocytes, all of which were significantly alleviated by the combination therapy with suboptimal ..
  32. Tang F, Qu M, Wang L, Ruan Y, Lu T, Zhang H, et al. Case-control association study of the 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) gene and schizophrenia in the Han Chinese population. Neurosci Lett. 2007;416:113-6 pubmed
    ..The expression of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), which is used as a myelin marker, has been reported to be reduced in the schizophrenic brain...
  33. Cho S, Jung J, Shin S, Jin I, Ko B, Kim Kwon Y, et al. Nonspecific association of 2',3'-cyclic nucleotide 3'-phosphodiesterase with the rat forebrain postsynaptic density fraction. Exp Mol Med. 2003;35:486-93 pubmed
    ..CNP), a protein of unknown function in vivo, is abundantly expressed in myelinating glia in two isoforms, CNP1 and CNP2...
  34. Evans J, Youssef A, Yandle T, Lewis L, Nicholls M. Effects of endothelin-1 on release of adrenomedullin and C-type natriuretic peptide from individual human vascular endothelial cells. J Endocrinol. 2002;175:225-32 pubmed
    ..Three of these vasoactive factors are adrenomedullin, C-type natriuretic peptide (CNP) and endothelin-1 (ET-1), each of which is claimed to have important local effects...
  35. Kozlov G, Lee J, Gravel M, Ekiel I, Braun P, Gehring K. Assignment of the 1H, 13C and 15N resonances of the catalytic domain of the rat 2',3'-cyclic nucleotide 3'-phosphodiesterase. J Biomol NMR. 2002;22:99-100 pubmed
  36. Bernier L, Alvarez F, Norgard E, Raible D, Mentaberry A, Schembri J, et al. Molecular cloning of a 2',3'-cyclic nucleotide 3'-phosphodiesterase: mRNAs with different 5' ends encode the same set of proteins in nervous and lymphoid tissues. J Neurosci. 1987;7:2703-10 pubmed
    ..were used to identify, in a rat brain phage lambda gt11 expression library, cDNA clones encoding beta-galactosidase-CNP fusion proteins, some of which showed CNP activity...
  37. Lovato L, Cianti R, Gini B, Marconi S, Bianchi L, Armini A, et al. Transketolase and 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I isoforms are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol Cell Proteomics. 2008;7:2337-49 pubmed publisher
    ..Our immunomics approach shed new light on the autoimmune repertoire present in MuS patients revealing novel oligodendroglial and/or neuronal putative autoantigens with potential important pathogenic and diagnostic implications. ..
  38. Watanabe M, Sakurai Y, Ichinose T, Aikawa Y, Kotani M, Itoh K. Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes. J Neurosci Res. 2006;84:525-33 pubmed
    ..To provide direct evidence that CNP was recognized by mAb-Rip, rat Cnp1-transfected HEK293T cells were used for double-immunofluorescence staining with mAb-Rip and mAb-CNP...