carboxypeptidase E

Summary

Gene Symbol: carboxypeptidase E
Description: carboxypeptidase E
Alias: CARBE, Cph, carboxypeptidase E, carboxypeptidase H, enkephalin convertase, prohormone-processing carboxypeptidase
Species: rat
Products:     carboxypeptidase E

Top Publications

  1. Zhou A, Minami M, Zhu X, Bae S, Minthorne J, Lan J, et al. Altered biosynthesis of neuropeptide processing enzyme carboxypeptidase E after brain ischemia: molecular mechanism and implication. J Cereb Blood Flow Metab. 2004;24:612-22 pubmed
    ..investigated the impact of brain ischemia on the biosynthesis of a key neuropeptide-processing enzyme, carboxypeptidase E (CPE). The response to brain ischemia of animals that lacked an active CPE was also examined...
  2. Hook V, Loh Y. Carboxypeptidase B-like converting enzyme activity in secretory granules of rat pituitary. Proc Natl Acad Sci U S A. 1984;81:2776-80 pubmed
  3. Zheng M, Streck R, Scott R, Seidah N, Pintar J. The developmental expression in rat of proteases furin, PC1, PC2, and carboxypeptidase E: implications for early maturation of proteolytic processing capacity. J Neurosci. 1994;14:4656-73 pubmed
    ..Following endoproteolytic cleavage, the carboxyl-terminal basic amino acid residues are removed by carboxypeptidase E (CPE)...
  4. Billova S, Galanopoulou A, Seidah N, Qiu X, Kumar U. Immunohistochemical expression and colocalization of somatostatin, carboxypeptidase-E and prohormone convertases 1 and 2 in rat brain. Neuroscience. 2007;147:403-18 pubmed
    ..Taken together, our data anticipate the possibility that CPE, PC1 and PC2 might be potential target for the maturation of SST. ..
  5. Srinivasan S, Bunch D, Feng Y, Rodriguiz R, Li M, Ravenell R, et al. Deficits in reproduction and pro-gonadotropin-releasing hormone processing in male Cpefat mice. Endocrinology. 2004;145:2023-34 pubmed
    Cpe(fat/fat) mice are obese, diabetic, and infertile. These animals have a point mutation in carboxypeptidase E (CPE), an exopeptidase that removes C-terminal basic amino acids from peptide intermediates...
  6. Park J, Cawley N, Loh Y. A bi-directional carboxypeptidase E-driven transport mechanism controls BDNF vesicle homeostasis in hippocampal neurons. Mol Cell Neurosci. 2008;39:63-73 pubmed publisher
    ..In our study, overexpression of the cytoplasmic tail of the carboxypeptidase E (CPE) found in BDNF vesicles significantly reduced localization of BDNF in neurites of hippocampal neurons...
  7. Oiso S, Takeda Y, Futagawa T, Miura T, Kuchiiwa S, Nishida K, et al. Contactin-associated protein (Caspr) 2 interacts with carboxypeptidase E in the CNS. J Neurochem. 2009;109:158-67 pubmed publisher
    ..We identified carboxypeptidase E (CPE) as a Caspr2-interacting candidate protein...
  8. Pance A, Morgan K, Guest P, Bowers K, Dean G, Cutler D, et al. A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression. J Neurochem. 1999;73:21-30 pubmed
    ..The proprotein-converting enzyme carboxypeptidase H was synthesised in the A35C cell line but was secreted by the constitutive pathway...
  9. Carrel D, Du Y, Komlos D, Hadzimichalis N, Kwon M, Wang B, et al. NOS1AP regulates dendrite patterning of hippocampal neurons through a carboxypeptidase E-mediated pathway. J Neurosci. 2009;29:8248-58 pubmed publisher
    ..Furthermore, we performed a yeast two-hybrid screen and identified carboxypeptidase E (CPE) as a binding partner for the middle region of NOS1AP-L...

More Information

Publications33

  1. Wu C, Chang H, Chang M. Membrane-bound carboxypeptidase E facilitates the entry of eosinophil cationic protein into neuroendocrine cells. Biochem J. 2004;382:841-8 pubmed
    ..and immunoprecipitation, we have identified a specific protein-protein interaction between mECP and CPE (carboxypeptidase E), a well characterized metalloprotease...
  2. Davidson H, Hutton J. The insulin-secretory-granule carboxypeptidase H. Purification and demonstration of involvement in proinsulin processing. Biochem J. 1987;245:575-82 pubmed
    ..The insulin-secretory-granule carboxypeptidase shared several properties of carboxypeptidase H from bovine adrenal medulla and pituitary...
  3. Murthy S, Dupart E, Al Sweel N, Chen A, Cawley N, Loh Y. Carboxypeptidase E promotes cancer cell survival, but inhibits migration and invasion. Cancer Lett. 2013;341:204-13 pubmed publisher
    b>Carboxypeptidase E (CPE), a prohormone processing enzyme is highly expressed and secreted from (neuro)endocrine tumors and gliomas, and has been implicated in cancer progression by promoting tumor growth...
  4. Manser E, Fernandez D, Loo L, Goh P, Monfries C, Hall C, et al. Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro. Biochem J. 1990;267:517-25 pubmed
    b>Carboxypeptidase E (CPE), which cleaves C-terminal amino acid residues and is involved in neuropeptide processing, is itself subject to intracellular processing...
  5. Naggert J, Fricker L, Varlamov O, Nishina P, Rouille Y, Steiner D, et al. Hyperproinsulinaemia in obese fat/fat mice associated with a carboxypeptidase E mutation which reduces enzyme activity. Nat Genet. 1995;10:135-42 pubmed
    ..The fat mutation maps to mouse chromosome 8, very close to the gene for carboxypeptidase E (Cpe), which encodes an enzyme (CPE) that processes prohormone intermediates such as proinsulin...
  6. Pritchard L, Turnbull A, White A. Pro-opiomelanocortin processing in the hypothalamus: impact on melanocortin signalling and obesity. J Endocrinol. 2002;172:411-21 pubmed
    ..We predict that further research into hypothalamic POMC processing, and the proteolytic enzymes involved, may yield important new clues on how flux through the MC4R pathway is regulated. ..
  7. Castano L, Russo E, Zhou L, Lipes M, Eisenbarth G. Identification and cloning of a granule autoantigen (carboxypeptidase-H) associated with type I diabetes. J Clin Endocrinol Metab. 1991;73:1197-201 pubmed
    ..Analysis of the sequence reveals that the clone encodes a 136-amino acid fragment of carboxypeptidase-H (enkephalin convertase). Carboxypeptidase-H is a molecule expressed within islet secretory granules and neurendocrine cells...
  8. Fricker L, Adelman J, Douglass J, Thompson R, Von Strandmann R, Hutton J. Isolation and sequence analysis of cDNA for rat carboxypeptidase E [EC 3.4.17.10], a neuropeptide processing enzyme. Mol Endocrinol. 1989;3:666-73 pubmed
    b>Carboxypeptidase E (CPE) is the carboxypeptidase B-like enzyme associated with the biosynthesis of numerous peptide hormones and neurotransmitters...
  9. Hosaka M, Watanabe T, Sakai Y, Kato T, Takeuchi T. Interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules. J Cell Sci. 2005;118:4785-95 pubmed
    Secretogranin III (SgIII) and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes. We previously showed that SgIII binds chromogranin A (CgA) and targets CgA to the SGs in endocrine cells...
  10. Arnett D, Devereux R, Rao D, Li N, Tang W, Kraemer R, et al. Novel genetic variants contributing to left ventricular hypertrophy: the HyperGEN study. J Hypertens. 2009;27:1585-93 pubmed publisher
    ..05). Genetic variation in NPY1R, NPY2R, NPY5R, CPE, IL15, and SFRP2, detected using linkage analysis in hypertensive siblings, was associated with left ventricular phenotypes in blacks and/or whites. ..
  11. Song L, Fricker L. The pro region is not required for the expression or intracellular routeing of carboxypeptidase E. Biochem J. 1997;323 ( Pt 1):265-71 pubmed
    b>Carboxypeptidase E (CPE) is initially synthesized as a larger precursor containing an additional 14-residue propeptide that is highly conserved between human and rat...
  12. Hougaard D, Larsson L. Carboxypeptidase E in rat antropyloric mucosa: distribution in progenitor and mature endocrine cell types. Histochem Cell Biol. 2004;121:55-61 pubmed
    ..It is believed that carboxypeptidase E (CPE) is involved in gut hormone processing but its presence in gut endocrine cells has never been studied...
  13. Woronowicz A, Koshimizu H, Chang S, Cawley N, Hill J, Rodriguiz R, et al. Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits. Hippocampus. 2008;18:1051-63 pubmed publisher
    ..Here we show that absence of the proneuropeptide processing enzyme, carboxypeptidase E (CPE) in CPE knock-out (KO) mice had a profound effect on memory, synaptic physiology, and the ..
  14. Hosaka M, Suda M, Sakai Y, Izumi T, Watanabe T, Takeuchi T. Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A. J Biol Chem. 2004;279:3627-34 pubmed
    ..SgIII and CgA were solubilized from the SGM by Triton X-100 in contrast to the Triton X-100 insolubility of carboxypeptidase E. SgIII and carboxypeptidase E strongly bound to the SGM-type liposome in intragranular conditions, but CgA ..
  15. Tang S, Zhang J, Liu H, Li H. PC2/CPE-mediated pro-protein processing in tumor cells and its differentiated cells or tissues. Mol Cell Endocrinol. 2009;303:43-9 pubmed publisher
    ..The results also showed that the artificial differentiation of RGC-5 or NS20Y cells was different from maturation of its corresponding normal tissue. ..
  16. Chen H, Jawahar S, Qian Y, Duong Q, Chan G, Parker A, et al. Missense polymorphism in the human carboxypeptidase E gene alters enzymatic activity. Hum Mutat. 2001;18:120-31 pubmed
    b>Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin...
  17. Utsunomiya N, Ohagi S, Sanke T, Tatsuta H, Hanabusa T, Nanjo K. Organization of the human carboxypeptidase E gene and molecular scanning for mutations in Japanese subjects with NIDDM or obesity. Diabetologia. 1998;41:701-5 pubmed
    ..and C-peptide by the concerted action of prohormone convertase 2 (PC2), prohormone convertase 3 (PC3) and carboxypeptidase E (CPE)...
  18. Varlamov O, Fricker L. The C-terminal region of carboxypeptidase E involved in membrane binding is distinct from the region involved with intracellular routing. J Biol Chem. 1996;271:6077-83 pubmed
    b>Carboxypeptidase E (CPE) is involved in the biosynthesis of numerous peptide hormones and neurotransmitters...
  19. Rodriguez C, Brayton K, Brownstein M, Dixon J. Rat preprocarboxypeptidase H. Cloning, characterization, and sequence of the cDNA and regulation of the mRNA by corticotropin-releasing factor. J Biol Chem. 1989;264:5988-95 pubmed
    b>Carboxypeptidase H is a putative post-translational processing enzyme which removes basic amino acid residues from intermediates during protein hormone biosynthesis. A 2...
  20. Qian Y, Varlamov O, Fricker L. Glu300 of rat carboxypeptidase E is essential for enzymatic activity but not substrate binding or routing to the regulated secretory pathway. J Biol Chem. 1999;274:11582-6 pubmed
    Several recently discovered members of the carboxypeptidase E (CPE) gene family lack critical active site residues that are conserved in other family members...
  21. Skalka N, Caspi M, Caspi E, Loh Y, Rosin Arbesfeld R. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene. 2013;32:2836-47 pubmed publisher
    ..Here we identify carboxypeptidase E (|CPE) and its splice variant, ΔN-CPE, as novel regulators of the Wnt pathway...
  22. Qin X, Cheng Y, Murthy S, Selvaraj P, Loh Y. carboxypeptidase E-ΔN, a neuroprotein transiently expressed during development protects embryonic neurons against glutamate neurotoxicity. PLoS ONE. 2014;9:e112996 pubmed publisher
    ..We have identified a novel protein, carboxypeptidase E-ΔN (CPE-ΔN), which is a splice variant of CPE that has neuroprotective effects on embryonic neurons...
  23. Jung Y, Kunczt C, Pearson R, Dixon J, Fricker L. Structural characterization of the rat carboxypeptidase-E gene. Mol Endocrinol. 1991;5:1257-68 pubmed
  24. Zhang H, Li S, Wang M, Vukusic B, Pristupa Z, Liu F. Regulation of dopamine transporter activity by carboxypeptidase E. Mol Brain. 2009;2:10 pubmed publisher
    ..Here, we have identified that carboxypeptidase E (CPE), a prohormone processing exopeptidase and sorting receptor for the regulated secretory pathway, ..