C5

Summary

Gene Symbol: C5
Description: complement C5
Alias: C5a, RGD1561905, complement C5, anaphylatoxin, complement component 5
Species: rat
Products:     C5

Top Publications

  1. Mulligan M, Schmid E, Beck Schimmer B, Till G, Friedl H, Brauer R, et al. Requirement and role of C5a in acute lung inflammatory injury in rats. J Clin Invest. 1996;98:503-12 pubmed
    The complement activation product, C5a, may play a key role in the acute inflammatory response...
  2. Huber Lang M, Younkin E, Sarma J, McGuire S, Lu K, Guo R, et al. Complement-induced impairment of innate immunity during sepsis. J Immunol. 2002;169:3223-31 pubmed
    ..The appearance of these defects was prevented by in vivo blockade of C5a in CLP rats...
  3. Hasegawa K, Tamari M, Shao C, Shimizu M, Takahashi N, Mao X, et al. Variations in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma. Hum Genet. 2004;115:295-301 pubmed
    ..to BA, we screened single nucleotide polymorphisms (SNPs) in C3, C5, the C3a receptor gene (C3AR1), and the C5a receptor gene (C5R1) and performed association studies in the Japanese population...
  4. Karp C, Grupe A, Schadt E, Ewart S, Keane Moore M, Cuomo P, et al. Identification of complement factor 5 as a susceptibility locus for experimental allergic asthma. Nat Immunol. 2000;1:221-6 pubmed
    ..Interleukin 12 (IL-12) is able to prevent or reverse experimental allergic asthma. Blockade of the C5a receptor rendered human monocytes unable to produce IL-12, mimicking blunted IL-12 production by macrophages from ..
  5. Crane J, Buller K. Systemic blockade of complement C5a receptors reduces lipopolysacharride-induced responses in the paraventricular nucleus and the central amygdala. Neurosci Lett. 2007;424:10-5 pubmed
    The complement anaphylatoxin C5a is a potent mediator of the innate immune response to infection. Recent evidence also reveals that C5a contributes to central nervous system effects in addition to its well-known peripheral functions...
  6. Flierl M, Rittirsch D, Chen A, Nadeau B, Day D, Sarma J, et al. The complement anaphylatoxin C5a induces apoptosis in adrenomedullary cells during experimental sepsis. PLoS ONE. 2008;3:e2560 pubmed publisher
    ..One of the cascades crucially involved in its pathogenesis is the complement system. Especially the anaphylatoxin C5a has been shown to have numerous harmful effects during sepsis...
  7. Woodruff T, Strachan A, Dryburgh N, Shiels I, Reid R, Fairlie D, et al. Antiarthritic activity of an orally active C5a receptor antagonist against antigen-induced monarticular arthritis in the rat. Arthritis Rheum. 2002;46:2476-85 pubmed
    To determine if the new, orally active C5a receptor antagonist, the cyclic peptide AcF-[OPdChaWR], reduces the severity of pathology in a rat model of immune-mediated monarticular arthritis...
  8. Xi G, Hua Y, Keep R, Younger J, Hoff J. Systemic complement depletion diminishes perihematomal brain edema in rats. Stroke. 2001;32:162-7 pubmed
    ..Immunohistochemistry was used to detect complement C3d, C5a, C9, and myeloperoxidase. Perihematomal brain edema was reduced by systemic complement depletion at 24 hours (78...
  9. Walter L, Hurt P, Himmelbauer H, Sudbrak R, Günther E. Physical mapping of the major histocompatibility complex class II and class III regions of the rat. Immunogenetics. 2002;54:268-75 pubmed
    ..Thus, the MHC of the rat is now completely mapped in a single contig of BAC/PAC clones derived from a single RT1 haplotype and encompasses about 3.7 Mb...

More Information

Publications62

  1. Ambati J, Anand A, Fernandez S, Sakurai E, Lynn B, Kuziel W, et al. An animal model of age-related macular degeneration in senescent Ccl-2- or Ccr-2-deficient mice. Nat Med. 2003;9:1390-7 pubmed
    ..RPE or choroidal endothelial production of Ccl-2 induced by complement C5a and IgG may mediate choroidal macrophage infiltration into aged wild-type choroids...
  2. Marc M, Kristan S, Rozman A, Kern I, Flezar M, Kosnik M, et al. Complement factor C5a in acute exacerbation of Chronic Obstructive Pulmonary Disease. Scand J Immunol. 2010;71:386-91 pubmed publisher
    The complement component C5a is a potent inflammatory peptide, which may be involved in the pathogenesis of Chronic Obstructive Pulmonary Disease (COPD)...
  3. Bhatia M, Saluja A, Singh V, Frossard J, Lee H, Bhagat L, et al. Complement factor C5a exerts an anti-inflammatory effect in acute pancreatitis and associated lung injury. Am J Physiol Gastrointest Liver Physiol. 2001;280:G974-8 pubmed
    Complement factor C5a acting via C5a receptors (C5aR) is recognized as an anaphylotoxin and chemoattractant that exerts proinflammatory effects in many pathological states...
  4. Dalle Lucca J, Chavko M, Dubick M, Adeeb S, Falabella M, Slack J, et al. Blast-induced moderate neurotrauma (BINT) elicits early complement activation and tumor necrosis factor ? (TNF?) release in a rat brain. J Neurol Sci. 2012;318:146-54 pubmed publisher
    ..Complement and TNF? activation may be a novel therapeutic target for reducing the damaging effects of BINT inflammation. ..
  5. Tang K, Cheng Y, Wu S, Liu L, Cheng L. Protective effect of C5 shRNA on myocardial ischemia-reperfusion injury in rats. Can J Physiol Pharmacol. 2012;90:1394-402 pubmed publisher
    ..Complement activation generates a series of bioactive substances, including early (C3a, C3b) and terminal (C5a, C5b-9) components. The terminal complement components are key mediators of MI/R injury...
  6. Ting E, Guerrero A, Cunha T, Verri W, Taylor S, Woodruff T, et al. Role of complement C5a in mechanical inflammatory hypernociception: potential use of C5a receptor antagonists to control inflammatory pain. Br J Pharmacol. 2008;153:1043-53 pubmed
    b>C5a, a complement activation product, exhibits a broad spectrum of inflammatory activities particularly neutrophil chemoattraction...
  7. Rubins J, Charboneau D, Paton J, Mitchell T, Andrew P, Janoff E. Dual function of pneumolysin in the early pathogenesis of murine pneumococcal pneumonia. J Clin Invest. 1995;95:142-50 pubmed
    ..Moreover, both the cytotoxic and the complement-activating activities of pneumolysin may contribute independently to the acute pulmonary injury and the high rates of bacteremia which characterize pneumococcal pneumonia. ..
  8. Robbins R, Russ W, Rasmussen J, Clayton M. Activation of the complement system in the adult respiratory distress syndrome. Am Rev Respir Dis. 1987;135:651-8 pubmed
    ..Because activation of the complement system can generate C5a, a potent neutrophil chemoattractant, complement activation was assessed in both serum and bronchoalveolar lavage ..
  9. Saito H, Lai J, Rogers R, Doerschuk C. Mechanical properties of rat bone marrow and circulating neutrophils and their responses to inflammatory mediators. Blood. 2002;99:2207-13 pubmed
    ..Studies using colchicine demonstrated that rearrangement of microtubules was not required for any of these processes but was required for normal trafficking of neutrophils through the pulmonary capillaries. ..
  10. Rhein L, Perkins M, Gerard N, Gerard C. FcgammaRIII is protective against Pseudomonas aeruginosa pneumonia. Am J Respir Cell Mol Biol. 2008;38:401-6 pubmed
    ..in C5, or targeted deletion of the C5a receptor, Pseudomonas lung infections reveal a role for the C5a anaphylatoxin in disease severity...
  11. Pierangeli S, Girardi G, Vega Ostertag M, Liu X, Espinola R, Salmon J. Requirement of activation of complement C3 and C5 for antiphospholipid antibody-mediated thrombophilia. Arthritis Rheum. 2005;52:2120-4 pubmed
    ..Mice deficient in complement components C3 and C5 were resistant to the enhanced thrombosis and endothelial cell activation that was induced by aPL...
  12. Hillebrandt S, Wasmuth H, Weiskirchen R, Hellerbrand C, Keppeler H, Werth A, et al. Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans. Nat Genet. 2005;37:835-43 pubmed
    ..Small molecule inhibitors of the C5a receptor had antifibrotic effects in vivo, and common haplotype-tagging polymorphisms of the human gene C5 were ..
  13. Langlois P, Gawryl M. Accentuated formation of the terminal C5b-9 complement complex in patient plasma precedes development of the adult respiratory distress syndrome. Am Rev Respir Dis. 1988;138:368-75 pubmed
    ..a more sensitive measure of this acute inflammatory lung injury than levels of C3a desarginine, C4a desarginine, C5a desarginine, and total hemolytic complement activity...
  14. Fick R, Robbins R, Squier S, Schoderbek W, Russ W. Complement activation in cystic fibrosis respiratory fluids: in vivo and in vitro generation of C5a and chemotactic activity. Pediatr Res. 1986;20:1258-68 pubmed
    ..chromatography and a sensitive radioimmunoassay the chemotaxin present in CF specimens was identified as the anaphylatoxin C5a...
  15. Short A, Paczkowski N, Vogen S, Sanderson S, Taylor S. Response-selective C5a agonists: differential effects on neutropenia and hypotension in the rat. Br J Pharmacol. 1999;128:511-4 pubmed
    Some in vivo activities of two complement C5a agonist analogues have been evaluated by measuring changes in blood pressure and neutropenia in the rat and comparing the results with their receptor affinities in peritoneal macrophages and ..
  16. Wilken H, Rogge S, Gotze O, Werfel T, Zwirner J. Specific detection by flow cytometry of histidine-tagged ligands bound to their receptors using a tag-specific monoclonal antibody. J Immunol Methods. 1999;226:139-45 pubmed
    ..Employing this method, a recombinant C3a (rC3a) anaphylatoxin with a His-tag at its N-terminus could be shown to bind to C3a receptor (C3aR)-expressing RBL-2H3 ..
  17. Hadders M, Bubeck D, Roversi P, Hakobyan S, Forneris F, Morgan B, et al. Assembly and regulation of the membrane attack complex based on structures of C5b6 and sC5b9. Cell Rep. 2012;1:200-7 pubmed publisher
    ..Cleavage of C5 to C5b results in marked conformational changes, distinct from those observed in the homologous C3-to-C3b transition...
  18. Tvedten H, Till G, Ward P. Mediators of lung injury in mice following systemic activation of complement. Am J Pathol. 1985;119:92-100 pubmed
    ..The marked attenuation of lung injury in C5-deficient mice indicates an absolute requirement for C5 in the development of lung injury...
  19. Vakeva A, Agah A, Rollins S, Matis L, Li L, Stahl G. Myocardial infarction and apoptosis after myocardial ischemia and reperfusion: role of the terminal complement components and inhibition by anti-C5 therapy. Circulation. 1998;97:2259-67 pubmed
    ..18A effectively blocked C5b-9-mediated cell lysis and C5a-induced chemotaxis of rat polymorphonuclear leukocytes (PMNs), whereas 16C had no complement inhibitor activity...
  20. Verroust F, Adam C, Kourilsky O, Allain J, Verroust P. Circulating immune complexes and complement levels in hemophilic children. J Clin Lab Immunol. 1981;6:127-30 pubmed
    ..Complement levels were elevated in a large percentage of patients, reaching statistical significance for C3, C4, C5, Factor B and Properdin...
  21. Boor P, Konieczny A, Villa L, Schult A, Bücher E, Rong S, et al. Complement C5 mediates experimental tubulointerstitial fibrosis. J Am Soc Nephrol. 2007;18:1508-15 pubmed
    ..in wild-type and C5(-/-) mice by unilateral ureteral ligation (UUO) and (2) investigation of the effects of a C5a receptor antagonist (C5aRA) in UUO...
  22. Flierl M, Perl M, Rittirsch D, Bartl C, Schreiber H, Fleig V, et al. The role of C5a in the innate immune response after experimental blunt chest trauma. Shock. 2008;29:25-31 pubmed
    ..There was a time-dependent systemic activation of complement as determined by CH-50 and presence of C5a-dependent chemotactic plasma activity...
  23. Fowler S, Seidenfeld J, Collins J, Kolb W. Induction of acute pulmonary inflammation. A differential effect of C5 and the 200,000 molecular weight form of trypsin-activated C5 (C5'). Am Rev Respir Dis. 1987;136:691-7 pubmed
    ..in which Syrian golden hamsters were injected intratracheally with 25 to 200 micrograms of highly purified human C5 or the 200,000 molecular weight form of trypsin-activated C5 (C5')...
  24. Buckingham S, Ramos T, Barnum S. Complement C5-deficient mice are protected from seizures in experimental cerebral malaria. Epilepsia. 2014;55:e139-42 pubmed publisher
    ..in the development of experimental cerebral malaria (ECM), we observed fewer seizures in mice deficient in C5, a component required for MAC formation...
  25. Nandakumar K, Jansson A, Xu B, Rydell N, Ahooghalandari P, Hellman L, et al. A recombinant vaccine effectively induces c5a-specific neutralizing antibodies and prevents arthritis. PLoS ONE. 2010;5:e13511 pubmed publisher
    ..and validate a recombinant vaccine to attenuate inflammation in arthritis by sustained neutralization of the anaphylatoxin C5a. We constructed and expressed fusion protein of C5a and maltose binding protein...
  26. Griffiths M, Wang J, Joe B, Dracheva S, Kawahito Y, Shepard J, et al. Identification of four new quantitative trait loci regulating arthritis severity and one new quantitative trait locus regulating autoantibody production in rats with collagen-induced arthritis. Arthritis Rheum. 2000;43:1278-89 pubmed
  27. Ren G, Huynh C, Bijian K, Cybulsky A. Role of apoptosis signal-regulating kinase 1 in complement-mediated glomerular epithelial cell injury. Mol Immunol. 2008;45:2236-46 pubmed publisher
    ..e. when ASK1 is overexpressed, p38 activation is amplified, and C5b-9 assembly leads to GEC injury via ASK1 and p38. The present study thus defines a novel role for ASK1 as a mediator of C5b-9-dependent cell injury. ..
  28. Proctor L, Moore T, Monk P, Sanderson S, Taylor S, Woodruff T. Complement factors C3a and C5a have distinct hemodynamic effects in the rat. Int Immunopharmacol. 2009;9:800-6 pubmed publisher
    In the rat, C5a infusion mediates well-defined effects including hypotension and neutropenia. Conversely, the comparative effect of C3a in the rat is not yet defined...
  29. Riedemann N, Guo R, Hollmann T, Gao H, Neff T, Reuben J, et al. Regulatory role of C5a in LPS-induced IL-6 production by neutrophils during sepsis. FASEB J. 2004;18:370-2 pubmed
    ..The proinflammatory mediator IL-6 has recently been shown to be an important inducer of the C5a receptor (C5aR) during sepsis...
  30. Lajoie S, Lewkowich I, Suzuki Y, Clark J, Sproles A, Dienger K, et al. Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma. Nat Immunol. 2010;11:928-35 pubmed publisher
    ..The opposing effects of C3a and C5a were mediated through their reciprocal regulation of IL-23 production...
  31. Cui L, Carney D, Hugli T. Primary structure and functional characterization of rat C5a: an anaphylatoxin with unusually high potency. Protein Sci. 1994;3:1169-77 pubmed
    The anaphylatoxin C5a is a pro-inflammatory factor generated from C5 during complement activation. C5a derived from rat C5 exhibits significantly greater potency compared to C5a from other species...
  32. Akatsu H, Miwa T, Sakurada C, Fukuoka Y, Ember J, Yamamoto T, et al. cDNA cloning and characterization of rat C5a anaphylatoxin receptor. Microbiol Immunol. 1997;41:575-80 pubmed
    Activation of the complement cascade plays an essential role in the early stages of inflammation. C5a and its receptor are particularly active in anaphylaxis...
  33. Schieferdecker H, Pestel S, Püschel G, Gotze O, Jungermann K. Increase by anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells: direct action of prostaglandins and indirect action of thromboxane A(2) on hepatocytes. Hepatology. 1999;30:454-61 pubmed
    In the perfused rat liver the anaphylatoxin C5a enhanced glucose output, reduced flow, and elevated prostanoid overflow. Because hepatocytes (HCs) do not express C5a receptors, the metabolic C5a actions must be indirect, mediated by e.g...
  34. Kondo C, Mizuno M, Nishikawa K, Yuzawa Y, Hotta N, Matsuo S. The role of C5a in the development of thrombotic glomerulonephritis in rats. Clin Exp Immunol. 2001;124:323-9 pubmed
    ..The aim of this work was to investigate the role of complement, especially an anaphylatoxin C5a, in a rat model of thrombotic glomerulonephritis...
  35. Montalto M, Hart M, Jordan J, Wada K, Stahl G. Role for complement in mediating intestinal nitric oxide synthase-2 and superoxide dismutase expression. Am J Physiol Gastrointest Liver Physiol. 2003;285:G197-206 pubmed
    ..An inhibitory or noninhibitory anti-complement component 5 (C5) monoclonal antibody (18A or 16C, respectively) was administered before GI/R...
  36. Riedemann N, Guo R, Sarma V, Laudes I, Huber Lang M, Warner R, et al. Expression and function of the C5a receptor in rat alveolar epithelial cells. J Immunol. 2002;168:1919-25 pubmed
    ..In the current studies, primary cultures of rat AEC (RAEC) have been shown to specifically bind recombinant rat C5a at high affinity and in a saturable manner...
  37. Flierl M, Stahel P, Rittirsch D, Huber Lang M, Niederbichler A, Hoesel L, et al. Inhibition of complement C5a prevents breakdown of the blood-brain barrier and pituitary dysfunction in experimental sepsis. Crit Care. 2009;13:R12 pubmed publisher
    ..However, its pathophysiology remains unclear. The present study investigated the effect of complement C5a blockade in preventing BBB damage and pituitary dysfunction during experimental sepsis...
  38. Kelly K, Liu Y, Zhang J, Dominguez J. Renal C3 complement component: feed forward to diabetic kidney disease. Am J Nephrol. 2015;41:48-56 pubmed publisher
    ..Moreover, mRNAs encoding the receptors for the anaphylatoxins C3a and C5a were also significantly increased in DI compared to LS...
  39. Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, et al. Sublytic C5b-9 induces IL-6 and TGF-?1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBP? acetylation. FASEB J. 2014;28:1511-25 pubmed publisher
  40. Auger J, Haasken S, Binstadt B. Autoantibody-mediated arthritis in the absence of C3 and activating Fc? receptors: C5 is activated by the coagulation cascade. Arthritis Res Ther. 2012;14:R269 pubmed publisher
    ..The three main pathways of complement activation converge at C3. However, C3-independent pathways can activate C5 and other downstream complement components during IgG-initiated inflammatory responses...
  41. Ruseva M, Hughes T, Donev R, Sivasankar B, Pickering M, Wu X, et al. Crry deficiency in complement sufficient mice: C3 consumption occurs without associated renal injury. Mol Immunol. 2009;46:803-11 pubmed publisher
    ..In order to rescue viable Crry(-/-) mice without deleting C3, we have tested inhibition of C5 during gestation...
  42. Nauta A, Daha M, Tijsma O, van de Water B, Tedesco F, Roos A. The membrane attack complex of complement induces caspase activation and apoptosis. Eur J Immunol. 2002;32:783-92 pubmed
    ..Apoptosis as a consequence of complement-mediated cell damage may provide an explanation for the presence of apoptosis in inflammatory processes, for instance in hyperacute xenograft rejection. ..
  43. Arumugam T, Woodruff T, Stocks S, Proctor L, Pollitt S, Shiels I, et al. Protective effect of a human C5a receptor antagonist against hepatic ischaemia-reperfusion injury in rats. J Hepatol. 2004;40:934-41 pubmed
    Complement activation is induced by ischaemia-reperfusion (I/R) and the complement factor C5a plays an important role in organ specific I/R injuries...
  44. Daveau M, Benard M, Scotte M, Schouft M, Hiron M, Francois A, et al. Expression of a functional C5a receptor in regenerating hepatocytes and its involvement in a proliferative signaling pathway in rat. J Immunol. 2004;173:3418-24 pubmed
    Activation of the complement system generates the anaphylatoxin C5a whose activities are mediated through its binding to the widely expressed C5aR...
  45. Melendi G, Hoffman S, Karron R, Irusta P, Laham F, Humbles A, et al. C5 modulates airway hyperreactivity and pulmonary eosinophilia during enhanced respiratory syncytial virus disease by decreasing C3a receptor expression. J Virol. 2007;81:991-9 pubmed
    ..Mice with a targeted disruption of complement component C5 affected by the enhanced disease displayed enhanced airway reactivity, lung eosinophilia, and mucus production ..
  46. Costa C, Zhao L, Shen Y, Su X, Hao L, Colgan S, et al. Role of complement component C5 in cerebral ischemia/reperfusion injury. Brain Res. 2006;1100:142-51 pubmed
    We evaluated the role of complement component C5 during the course of cerebral ischemic reperfusion injury in a rat model of middle cerebral artery occlusion (MCAO)...
  47. Abe M, Shibata K, Akatsu H, Shimizu N, Sakata N, Katsuragi T, et al. Contribution of anaphylatoxin C5a to late airway responses after repeated exposure of antigen to allergic rats. J Immunol. 2001;167:4651-60 pubmed
    ..Premedication with a C5a receptor antagonist (C5aRA) before every exposure to OVA also inhibited the LAR after three...
  48. Dromer F, Perronne C, Barge J, Vilde J, Yeni P. Role of IgG and complement component C5 in the initial course of experimental cryptococcosis. Clin Exp Immunol. 1989;78:412-7 pubmed
    ..capsular polysaccharide, and mice congenitally sufficient or deficient in the fifth component of complement (C5)...
  49. Schlaf G, Schieferdecker H, Rothermel E, Jungermann K, Gotze O. Differential expression of the C5a receptor on the main cell types of rat liver as demonstrated with a novel monoclonal antibody and by C5a anaphylatoxin-induced Ca2+ release. Lab Invest. 1999;79:1287-97 pubmed
    The C5-anaphylatoxin (C5a) is a protein of 74 (human) or 77 (rat) amino acid residues, respectively, which is generated by limited proteolysis upon activation of the fifth component of complement...
  50. Woodruff T, Arumugam T, Shiels I, Reid R, Fairlie D, Taylor S. Protective effects of a potent C5a receptor antagonist on experimental acute limb ischemia-reperfusion in rats. J Surg Res. 2004;116:81-90 pubmed
    The capacity of a potent C5a receptor antagonist to inhibit various parameters of local and remote organ injury following lower limb ischemia-reperfusion (I/R) in rats was investigated...
  51. Short A, Wong A, Finch A, Haaima G, Shiels I, Fairlie D, et al. Effects of a new C5a receptor antagonist on C5a- and endotoxin-induced neutropenia in the rat. Br J Pharmacol. 1999;126:551-4 pubmed
    A new C5a receptor antagonist, the cyclic peptide Phe-[Orn-Pro-D-cyclohexylalanine-Trp-Arg], (F-[OPdChaWR]), was tested for its ability to antagonize the neutropenic effects of both C5a and endotoxin in rats...
  52. Nozaki M, Raisler B, Sakurai E, Sarma J, Barnum S, Lambris J, et al. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A. 2006;103:2328-33 pubmed
    ..Here we provide evidence that bioactive fragments of these complement components (C3a and C5a) are present in drusen of patients with AMD, and that C3a and C5a induce VEGF expression in vitro and in vivo...
  53. Johswich K, Martin M, Thalmann J, Rheinheimer C, Monk P, Klos A. Ligand specificity of the anaphylatoxin C5L2 receptor and its regulation on myeloid and epithelial cell lines. J Biol Chem. 2006;281:39088-95 pubmed
    During complement activation the pro-inflammatory anaphylatoxins C3a and C5a are generated, which interact with the C3a receptor and C5a receptor (CD88), respectively...