Gene Symbol: Atp1a1
Description: ATPase Na+/K+ transporting subunit alpha 1
Alias: Nkaa1b, sodium/potassium-transporting ATPase subunit alpha-1, ATPase, Na+/K+ transporting, alpha 1 polypeptide, ATPase, Na+K+ transporting, alpha 1, Na(+)/K(+) ATPase alpha-1 subunit, sodium pump subunit alpha-1
Species: rat
Products:     Atp1a1

Top Publications

  1. Li C, Capendeguy O, Geering K, Horisberger J. A third Na+-binding site in the sodium pump. Proc Natl Acad Sci U S A. 2005;102:12706-11 pubmed
    ..amp; Toyoshima, C. (2002) Proc. Natl. Acad. Sci. USA 99, 15977-15982]. ..
  2. McLean W, Smith K, Glowatzki E, Pyott S. Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti. J Assoc Res Otolaryngol. 2009;10:37-49 pubmed publisher
  3. Shull G, Greeb J, Lingrel J. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain. Biochemistry. 1986;25:8125-32 pubmed
  4. Lencesova L, O Neill A, Resneck W, Bloch R, Blaustein M. Plasma membrane-cytoskeleton-endoplasmic reticulum complexes in neurons and astrocytes. J Biol Chem. 2004;279:2885-93 pubmed
    ..These PM and ER components appear to be linked through the cytoskeletal spectrin network, to which they are probably tethered by Ank 2. ..
  5. Devarajan P, Scaramuzzino D, Morrow J. Ankyrin binds to two distinct cytoplasmic domains of Na,K-ATPase alpha subunit. Proc Natl Acad Sci U S A. 1994;91:2965-9 pubmed
  6. Fisone G, Cheng S, Nairn A, Czernik A, Hemmings H, Hoog J, et al. Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K(+)-ATPase and effects of site-directed mutagenesis. J Biol Chem. 1994;269:9368-73 pubmed
    ..The results suggest that, in intact cells, the activity of the Na+,K(+)-ATPase is regulated in part by signal transduction pathways that use protein kinase A-dependent phosphorylation of the Na+,K(+)-ATPase alpha subunit. ..
  7. Feschenko M, Sweadner K. Structural basis for species-specific differences in the phosphorylation of Na,K-ATPase by protein kinase C. J Biol Chem. 1995;270:14072-7 pubmed
    ..It is also likely to be relevant to other known species-specific effects of protein kinase C on Na,K-ATPase. ..
  8. Dostanic Larson I, Van Huysse J, Lorenz J, Lingrel J. The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation. Proc Natl Acad Sci U S A. 2005;102:15845-50 pubmed
    ..Taken together these results demonstrate that the cardiac glycoside binding site of the alpha isoforms of the Na,K-ATPase have a physiological function and supports the hypothesis for a role of the endogenous cardiac glycosides...
  9. Liu L, Askari A. Beta-subunit of cardiac Na+-K+-ATPase dictates the concentration of the functional enzyme in caveolae. Am J Physiol Cell Physiol. 2006;291:C569-78 pubmed
    ..Uneven distributions of alpha(1) and beta(1) in early and late endosomes of myocytes suggested different internalization routes of two subunits as a source of selective localization of active Na(+)-K(+)-ATPase in cardiac caveolae. ..

More Information


  1. Morth J, Pedersen B, Toustrup Jensen M, Sørensen T, Petersen J, Andersen J, et al. Crystal structure of the sodium-potassium pump. Nature. 2007;450:1043-9 pubmed
    ..The carboxy terminus of the alpha-subunit is contained within a pocket between transmembrane helices and seems to be a novel regulatory element controlling sodium affinity, possibly influenced by the membrane potential. ..
  2. Takiguchi Y, Sun G, Ogawa K, Matsunaga T. Long-lasting changes in the cochlear K+ recycling structures after acute energy failure. Neurosci Res. 2013;77:33-41 pubmed publisher
    ..These results indicate long-lasting changes in the cochlear lateral wall and spiral limbus, which may compensate for damaged K(+) recycling and protect cells from ATP shortage. ..
  3. Kava L, Rossi N, Mattingly R, Yingst D. Increased expression of Na,K-ATPase and a selective increase in phosphorylation at Ser-11 in the cortex of the 2-kidney, 1-clip hypertensive rat. Am J Hypertens. 2012;25:487-91 pubmed publisher
    ..Thus, in the kidney cortex of rats with renovascular hypertension there is increased expression of Na,K-ATPase and a selective increase in its phosphorylation at Ser-11 that could increase the capacity to reabsorb sodium and water. ..
  4. Yan X, Xun M, Dou X, Wu L, Zhang F, Zheng J. Activation of Na+-K+-ATPase with DRm217 attenuates oxidative stress-induced myocardial cell injury via closing Na+-K+-ATPase/Src/Ros amplifier. Apoptosis. 2017;22:531-543 pubmed publisher
    ..These novel results may help us to understand the important role of the Na+-K+-ATPase in oxidative stress and oxidative stress-related disease. ..
  5. Crambert G, Schaer D, Roy S, Geering K. New molecular determinants controlling the accessibility of ouabain to its binding site in human Na,K-ATPase alpha isoforms. Mol Pharmacol. 2004;65:335-41 pubmed
  6. Macphee D, Jones D, Barr K, Betts D, Watson A, Kidder G. Differential involvement of Na(+),K(+)-ATPase isozymes in preimplantation development of the mouse. Dev Biol. 2000;222:486-98 pubmed
    ..Our findings imply that the regulation of sodium transport within the preimplantation mouse embryo is more complex than had been appreciated. ..
  7. Desfrere L, Karlsson M, Hiyoshi H, Malmersjö S, Nanou E, Estrada M, et al. Na,K-ATPase signal transduction triggers CREB activation and dendritic growth. Proc Natl Acad Sci U S A. 2009;106:2212-7 pubmed publisher
    ..Thus, our results suggest a novel role for the Na,K-ATPase as a modulator of dendritic growth in developing neurons. ..
  8. Stabach P, Devarajan P, Stankewich M, Bannykh S, Morrow J. Ankyrin facilitates intracellular trafficking of alpha1-Na+-K+-ATPase in polarized cells. Am J Physiol Cell Physiol. 2008;295:C1202-14 pubmed publisher
    ..This novel ankyrin-dependent assembly pathway suggests a mechanism whereby hereditary disorders of ankyrin may be manifested as diseases of membrane protein ER retention or mislocalization. ..
  9. Efendiev R, Cinelli A, Leibiger I, Bertorello A, Pedemonte C. FRET analysis reveals a critical conformational change within the Na,K-ATPase alpha1 subunit N-terminus during GPCR-dependent endocytosis. FEBS Lett. 2006;580:5067-70 pubmed
    ..This is consistent with a protein conformational change that results in the N-terminal end of alpha1 moving closer to the internal face of the plasma membrane. ..
  10. Qiu L, Koenderink J, Swarts H, Willems P, De Pont J. Phe783, Thr797, and Asp804 in transmembrane hairpin M5-M6 of Na+,K+-ATPase play a key role in ouabain binding. J Biol Chem. 2003;278:47240-4 pubmed
    ..Most likely, the presence of this amino acid is required for adopting of the proper conformation for ouabain binding. ..
  11. Crambert G, Hasler U, Beggah A, Yu C, Modyanov N, Horisberger J, et al. Transport and pharmacological properties of nine different human Na, K-ATPase isozymes. J Biol Chem. 2000;275:1976-86 pubmed
    ..In conclusion, our study reveals several new functional characteristics of human Na,K-ATPase isozymes which help to better understand their role in ion homeostasis in different tissues and in digitalis action and toxicity. ..
  12. Kao L, Kurtz L, Shao X, Papadopoulos M, Liu L, Bok D, et al. Severe neurologic impairment in mice with targeted disruption of the electrogenic sodium bicarbonate cotransporter NBCe2 (Slc4a5 gene). J Biol Chem. 2011;286:32563-74 pubmed publisher
    ..Our findings are the first demonstration of the fundamental importance of NBCe2 in the biology of the nervous system. ..
  13. Lei J, Mariash C, Ingbar D. 3,3',5-Triiodo-L-thyronine up-regulation of Na,K-ATPase activity and cell surface expression in alveolar epithelial cells is Src kinase- and phosphoinositide 3-kinase-dependent. J Biol Chem. 2004;279:47589-600 pubmed
  14. Silva V, Oliveira L, Gonçalves P. Alteration of aluminium inhibition of synaptosomal (Na(+)/K(+))ATPase by colestipol administration. J Inorg Biochem. 2013;128:208-14 pubmed publisher
    ..The aluminium inhibitory effect on synaptosomal (Na(+)/K(+))ATPase was reduced by cholesterol depletion both in vitro and in vivo...
  15. Efendiev R, Budu C, Bertorello A, Pedemonte C. G-protein-coupled receptor-mediated traffic of Na,K-ATPase to the plasma membrane requires the binding of adaptor protein 1 to a Tyr-255-based sequence in the alpha-subunit. J Biol Chem. 2008;283:17561-7 pubmed publisher
  16. Fujii T, Fujita K, Shimizu T, Takeguchi N, Sakai H. The NH(2)-terminus of K(+)-Cl(-) cotransporter 3a is essential for up-regulation of Na(+),K(+)-ATPase activity. Biochem Biophys Res Commun. 2010;399:683-7 pubmed publisher
    ..Our results suggest that the NH(2)-terminus of KCC3a is a key region for association with alpha1NaK, and that KCC3a but not KCC3b can regulate the Na(+),K(+)-ATPase activity. ..
  17. Einholm A, Andersen J, Vilsen B. Roles of transmembrane segment M1 of Na+,K+-ATPase and Ca2-ATPase, the gatekeeper and the pivot. J Bioenerg Biomembr. 2007;39:357-66 pubmed
    ..A pivot formed between a pair of a glycine and a bulky residue in M1 and M3 seems critical to the opening of the extracytoplasmic gate in both the Ca2+-ATPase and the Na+,K+-ATPase. ..
  18. Krenek P, Hamaide M, Morel N, Wibo M. A simple method for rapid separation of endothelial and smooth muscle mRNA reveals Na/K+ -ATPase alpha-subunit distribution in rat arteries. J Vasc Res. 2006;43:502-10 pubmed
  19. Hilge M, Siegal G, Vuister G, Güntert P, Gloor S, Abrahams J. ATP-induced conformational changes of the nucleotide-binding domain of Na,K-ATPase. Nat Struct Biol. 2003;10:468-74 pubmed
  20. Correll R, Eder P, Burr A, Despa S, Davis J, Bers D, et al. Overexpression of the Na+/K+ ATPase ?2 but not ?1 isoform attenuates pathological cardiac hypertrophy and remodeling. Circ Res. 2014;114:249-256 pubmed publisher
  21. Pritchard T, Parvatiyar M, Bullard D, Lynch R, Lorenz J, Paul R. Transgenic mice expressing Na+-K+-ATPase in smooth muscle decreases blood pressure. Am J Physiol Heart Circ Physiol. 2007;293:H1172-82 pubmed
    ..Our results show that smooth muscle displays unique coordinate expression of the alpha-isoforms. Increasing smooth muscle NKA decreases blood pressure and is dependent on the degree of increased alpha-isoform expression. ..
  22. Imagawa T, Kaya S, Taniguchi K. The amino acid sequence 442GDASE446 in Na/K-ATPase is an important motif in forming the high and low affinity ATP binding pockets. J Biol Chem. 2003;278:50283-92 pubmed
    ..These data and others indicate that the sequence 442GDASE446 in the ATP binding pocket is an important motif that it is involved in both the high and low affinity ATP effects rather than in free Mg2+, Na+, and K+ effects. ..
  23. Tsimaratos M, Roger F, Chabardes D, Mordasini D, Hasler U, Doucet A, et al. C-peptide stimulates Na+,K+-ATPase activity via PKC alpha in rat medullary thick ascending limb. Diabetologia. 2003;46:124-31 pubmed
  24. Avner E. Epithelial polarity and differentiation in polycystic kidney disease. J Cell Sci Suppl. 1993;17:217-22 pubmed
    ..If such apically expressed enzyme is active, it may have pathogenic import in collecting tubule cyst formation and enlargement by mediating net basal to apical vectorial solute and fluid transport. ..
  25. Rose E, Koo J, Antflick J, Ahmed S, Angers S, Hampson D. Glutamate transporter coupling to Na,K-ATPase. J Neurosci. 2009;29:8143-55 pubmed publisher
    ..These findings demonstrate that glutamate transporters and Na,K-ATPases are part of the same macromolecular complexes and operate as a functional unit to regulate glutamatergic neurotransmission...
  26. Schack V, Morth J, Toustrup Jensen M, Anthonisen A, Nissen P, Andersen J, et al. Identification and function of a cytoplasmic K+ site of the Na+, K+ -ATPase. J Biol Chem. 2008;283:27982-90 pubmed publisher
  27. Medford R, Hyman R, Ahmad M, Allen J, Pressley T, Allen P, et al. Vascular smooth muscle expresses a truncated Na+, K(+)-ATPase alpha-1 subunit isoform. J Biol Chem. 1991;266:18308-12 pubmed
  28. Ruiz Opazo N, Barany F, Hirayama K, Herrera V. Confirmation of mutant alpha 1 Na,K-ATPase gene and transcript in Dahl salt-sensitive/JR rats. Hypertension. 1994;24:260-70 pubmed
    ..This identifies a site- and nucleotide-specific Taq polymerase misincorporation, suggesting that a structural basis might underlie a predisposition to nonrandom Taq polymerase errors. ..
  29. Comellas A, Kelly A, Trejo H, Briva A, Lee J, Sznajder J, et al. Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt. J Cell Sci. 2010;123:1343-51 pubmed publisher
    ..Collectively, these results suggest that Akt plays a major role in Na(+)/K(+)-ATPase intracellular translocation and thus in alveolar fluid reabsorption. ..
  30. Mokry M, Cuppen E. The Atp1a1 gene from inbred Dahl salt sensitive rats does not contain the A1079T missense transversion. Hypertension. 2008;51:922-7 pubmed publisher
    The existence of the A1079T transversion in the alpha1 isoform of the Na(+), K(+)-ATPase (Atp1a1) gene in Dahl salt-sensitive rat (SS/Jr) strain, discovered by Herrera and Ruiz-Opazo and proposed to underlay hypertension sensitivity, ..
  31. Liu L, Zhao X, Pierre S, Askari A. Association of PI3K-Akt signaling pathway with digitalis-induced hypertrophy of cardiac myocytes. Am J Physiol Cell Physiol. 2007;293:C1489-97 pubmed
  32. Lee K, Jung J, Kim M, Guidotti G. Interaction of the alpha subunit of Na,K-ATPase with cofilin. Biochem J. 2001;353:377-85 pubmed
  33. Petrushanko I, Yakushev S, Mitkevich V, Kamanina Y, Ziganshin R, Meng X, et al. S-glutathionylation of the Na,K-ATPase catalytic ? subunit is a determinant of the enzyme redox sensitivity. J Biol Chem. 2012;287:32195-205 pubmed publisher
    ..The rat ?2 isoform was more sensitive to GSSG than the ?1 isoform. Our findings imply that regulatory S-glutathionylation of the catalytic subunit plays a key role in the redox-induced regulation of Na,K-ATPase activity. ..
  34. Ruete M, Carrizo L, Bocanegra M, Valles P. Altered renal expression of Na(+) transporters and ROMK in protein-deprived rats. Nephron Physiol. 2009;111:p17-29 pubmed publisher
    ..A role of aldosterone may be suggested. ..
  35. Kimura T, Han W, Pagel P, Nairn A, Caplan M. Protein phosphatase 2A interacts with the Na,K-ATPase and modulates its trafficking by inhibition of its association with arrestin. PLoS ONE. 2011;6:e29269 pubmed publisher
    ..Taken together, these data demonstrate that the sodium pump belongs to a growing list of ion transport proteins that are regulated through direct interactions with the catalytic subunit of a protein phosphatase. ..
  36. Chen Y, Cai T, Wang H, Li Z, Loreaux E, Lingrel J, et al. Regulation of intracellular cholesterol distribution by Na/K-ATPase. J Biol Chem. 2009;284:14881-90 pubmed publisher
    ..Moreover, the data also suggest that the plasma membrane Na/K-ATPase-caveolin-1 interaction may represent an important sensing mechanism by which the cells regulate the sterol regulatory element-binding protein pathway. ..
  37. Orsenigo M, Faelli A, Porta C, Sironi C, Laforenza U, Paulmichl M, et al. Oxidative stress reduces transintestinal transports and (Na+, K+) -ATPase activity in rat jejunum. Arch Biochem Biophys. 2007;466:300-7 pubmed
    ..We conclude that H(2)O(2) may be a modulator of jejunal ion and water transport by multiple mechanisms, among which a significant inhibition of the basolateral (Na+, K+) -ATPase. ..
  38. Pierre S, Duran M, Carr D, Pressley T. Structure/function analysis of Na(+)-K(+)-ATPase central isoform-specific region: involvement in PKC regulation. Am J Physiol Renal Physiol. 2002;283:F1066-74 pubmed
    ..Comparisons with rat full-length alpha(1)- and alpha(2)-isoforms expressed under the same conditions suggest an involvement of the central ISR in the response to PKC but not in K(+) deocclusion. ..
  39. Xu K, Takimoto E, Juang G, Zhang Q, Rohde H, Myers A. Evidence that the H1-H2 domain of alpha1 subunit of (Na++K+)-ATPase participates in the regulation of cardiac contraction. FASEB J. 2005;19:53-61 pubmed
    ..These new findings suggest that the H1-H2 domain of 1 subunit of NKA is a critical determinant of enzyme biologic activity, which couples to enhanced myocyte calcium transient and inotropic action. ..
  40. Schneider J, Mercer R, Caplan M, Emanuel J, Sweadner K, Benz E, et al. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA. Proc Natl Acad Sci U S A. 1985;82:6357-61 pubmed
    ..In a ouabain-resistant human cell line, on the other hand, ouabain resistance appears to involve an increase in the number of gene copies coding for the Na,K-ATPase. ..
  41. Petrushanko I, Bogdanov N, Bulygina E, Grenacher B, Leinsoo T, Boldyrev A, et al. Na-K-ATPase in rat cerebellar granule cells is redox sensitive. Am J Physiol Regul Integr Comp Physiol. 2006;290:R916-25 pubmed
    ..The obtained data indicate that redox state is a potent regulator of the Na-K-ATPase function. Shifts from an "optimal redox potential range" to higher or lower levels cause suppression of the Na-K pump activity. ..
  42. Li C, Crambert G, Thuillard D, Roy S, Schaer D, Geering K. Role of the transmembrane domain of FXYD7 in structural and functional interactions with Na,K-ATPase. J Biol Chem. 2005;280:42738-43 pubmed
    ..These amino acids act in a synergistic way. These results highlight the important structural and functional role of the TM domain of FXYD7 and delineate the determinants that mediate the complex interactions of FXYD7 with Na,K-ATPase. ..
  43. Gusarova G, Trejo H, Dada L, Briva A, Welch L, Hamanaka R, et al. Hypoxia leads to Na,K-ATPase downregulation via Ca(2+) release-activated Ca(2+) channels and AMPK activation. Mol Cell Biol. 2011;31:3546-56 pubmed publisher
    ..These data suggest that during hypoxia, calcium entry via CRAC channels leads to AMPK activation, Na,K-ATPase downregulation, and alveolar epithelial dysfunction. ..
  44. Costa C, Gatto C, Kaplan J. Interactions between Na,K-ATPase alpha-subunit ATP-binding domains. J Biol Chem. 2003;278:9176-84 pubmed
    ..Although any functional significance of these associations for ion transport remains unresolved, they may play a role in cell function and in modulating interactions between the Na,K-ATPase and other proteins. ..
  45. Goel M, Sinkins W, Keightley A, Kinter M, Schilling W. Proteomic analysis of TRPC5- and TRPC6-binding partners reveals interaction with the plasmalemmal Na(+)/K(+)-ATPase. Pflugers Arch. 2005;451:87-98 pubmed
    ..These observations suggest that TRPC6 and the Na(+) pump are part of a functional complex that may be involved in ion transport and homeostasis in both the brain and kidney. ..
  46. Berger G, Guetta J, Klorin G, Badarneh R, Braun E, Brod V, et al. Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump. Am J Physiol Lung Cell Mol Physiol. 2011;301:L23-30 pubmed publisher
    ..Early decrease in AFC in remote sepsis is probably related to endocytosis of the Na-K-ATPase proteins from the cell plasma membrane into intracellular pools, with resultant inhibition of active sodium transport in ATII cells. ..
  47. Jeremias I, Scaini G, Constantino L, Vuolo F, Ferreira A, Scherer E, et al. The decrease on Na(+), K(+)-ATPase activity in the cortex, but not in hippocampus, is reverted by antioxidants in an animal model of sepsis. Mol Neurobiol. 2012;46:467-74 pubmed publisher
    ..In conclusion, the present study demonstrated that sepsis induced by CLP inhibits Na(+), K(+)-ATPase activity in a mechanism dependent on oxidative stress, but this is not associated to increase brain water content. ..
  48. Massey K, Li Q, Rossi N, Mattingly R, Yingst D. Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase. Biochem J. 2012;443:249-58 pubmed publisher
    ..Ang II increased the initial rate and slowed the second phase in ?-1.wild-type, but not ?-1.S938A, cells. Thus Ang II changes the conformation of two forms of EP2 via differential phosphorylation. ..
  49. Despa S, Bers D. Functional analysis of Na+/K+-ATPase isoform distribution in rat ventricular myocytes. Am J Physiol Cell Physiol. 2007;293:C321-7 pubmed
    ..The functional density of NKA-alpha2 is approximately 4.5 times higher in the T-tubules vs. ESL, whereas NKA-alpha1 is almost uniformly distributed between the TT and ESL...
  50. Deng Y, Rapp J. Cosegregation of blood pressure with angiotensin converting enzyme and atrial natriuretic peptide receptor genes using Dahl salt-sensitive rats. Nat Genet. 1992;1:267-72 pubmed
    ..We conclude that certain alleles at the GCA and ACE loci (or at loci closely linked to them) have a significant genetic impact on blood pressure response to high salt in specific rat strains. ..
  51. Wild G, Searles L, Koski K, Drozdowski L, Begum Hasan J, Thomson A. Oral polyamine administration modifies the ontogeny of hexose transporter gene expression in the postnatal rat intestine. Am J Physiol Gastrointest Liver Physiol. 2007;293:G453-60 pubmed
    ..Thus, 3 days of oral polyamine administration induces the precocious maturation of glucose transporters in the postnatal rat small intestine, which may be mediated by alterations in ODC expression. ..
  52. Toustrup Jensen M, Vilsen B. Interaction between the catalytic site and the A-M3 linker stabilizes E2/E2P conformational states of Na+,K+-ATPase. J Biol Chem. 2005;280:10210-8 pubmed
  53. Bondarenko A, Sagach V. Na+-K+-ATPase is involved in the sustained ACh-induced hyperpolarization of endothelial cells from rat aorta. Br J Pharmacol. 2006;149:958-65 pubmed
    ..They also suggest that the alpha1, but not alpha2 or alpha3 isoforms, is involved in ACh-mediated hyperpolarization. ..
  54. Sjöström M, Stenström K, Eneling K, Zwiller J, Katz A, Takemori H, et al. SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc Natl Acad Sci U S A. 2007;104:16922-7 pubmed
    ..These observations illustrate the existence of a distinct intracellular signaling network, with SIK1 at its core, which is triggered by a monovalent cation (Na(+)) and links sodium permeability to its active transport. ..
  55. Lai F, Madan N, Ye Q, Duan Q, Li Z, Wang S, et al. Identification of a mutant ?1 Na/K-ATPase that pumps but is defective in signal transduction. J Biol Chem. 2013;288:13295-304 pubmed publisher
    ..It has not been possible to study the pumping and signaling functions of Na/K-ATPase independently in live cells...
  56. Hundal H, Maxwell D, Ahmed A, Darakhshan F, Mitsumoto Y, Klip A. Subcellular distribution and immunocytochemical localization of Na,K-ATPase subunit isoforms in human skeletal muscle. Mol Membr Biol. 1994;11:255-62 pubmed
    ..The beta 1 subunit was enriched in the PM fraction but was also detected to a modest extent in the IM.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  57. Dostanic I, Schultz J, Lorenz J, Lingrel J. The alpha 1 isoform of Na,K-ATPase regulates cardiac contractility and functionally interacts and co-localizes with the Na/Ca exchanger in heart. J Biol Chem. 2004;279:54053-61 pubmed
    ..These results demonstrate that the alpha 1 isoform regulates cardiac contractility, and that both the alpha 1 and alpha 2 isoforms are functionally and physically coupled with the Na/Ca exchanger in heart. ..
  58. Imagawa T, Yamamoto T, Kaya S, Sakaguchi K, Taniguchi K. Thr-774 (transmembrane segment M5), Val-920 (M8), and Glu-954 (M9) are involved in Na+ transport, and Gln-923 (M8) is essential for Na,K-ATPase activity. J Biol Chem. 2005;280:18736-44 pubmed
  59. Liu Y, Shoji Kawata S, Sumpter R, Wei Y, Ginet V, Zhang L, et al. Autosis is a Na+,K+-ATPase-regulated form of cell death triggered by autophagy-inducing peptides, starvation, and hypoxia-ischemia. Proc Natl Acad Sci U S A. 2013;110:20364-71 pubmed publisher
    ..These findings have implications for understanding how cells die during certain stress conditions and how such cell death might be prevented. ..
  60. Yin J, Guo H, Yu D, Wang H, Li J, Wang Y. Mechanisms of isoform-specific Na/K pump regulation by short- and long-term adrenergic activation in rat ventricular myocytes. Cell Physiol Biochem. 2014;33:1681-97 pubmed publisher
    ..Furthermore, long-term regulation involves transcription and translation of the respective ?-isoform, whereas short-term regulation involves the translocation of the available ?-isoform to the plasma membrane. ..
  61. Belliard A, Sottejeau Y, Duan Q, Karabin J, Pierre S. Modulation of cardiac Na+,K+-ATPase cell surface abundance by simulated ischemia-reperfusion and ouabain preconditioning. Am J Physiol Heart Circ Physiol. 2013;304:H94-103 pubmed publisher
    ..They further suggest that the protection conferred by increased surface expression of Na(+),K(+)-ATPase may be independent of ion transport. ..
  62. Dada L, Welch L, Zhou G, Ben Saadon R, Ciechanover A, Sznajder J. Phosphorylation and ubiquitination are necessary for Na,K-ATPase endocytosis during hypoxia. Cell Signal. 2007;19:1893-8 pubmed
    ..We provide evidence that ubiquitination plays an important role in cellular adaptation to hypoxia by regulating Na,K-ATPase alpha(1)-subunit endocytosis. ..
  63. Barnard R, Kelly G, Manzetti S, Harris E. Neither the New Zealand genetically hypertensive strain nor Dahl salt-sensitive strain has an A1079T transversion in the alpha1 isoform of the Na(+),K(+)-ATPase gene. Hypertension. 2001;38:786-92 pubmed
    A putative 1079A-->T mutation in the alpha1 isoform of the Na(+), K(+)-ATPase (Atp1a1) gene of the Dahl salt-sensitive rat inbred by John Rapp (SS/Jr) strain was projected to cause a conformation change in the membrane hydrophobic ..
  64. Ritter L, Kleemann D, Hickmann F, Amaral A, Sitta Ã, Wajner M, et al. Disturbance of energy and redox homeostasis and reduction of Na+,K+-ATPase activity provoked by in vivo intracerebral administration of ethylmalonic acid to young rats. Biochim Biophys Acta. 2015;1852:759-67 pubmed publisher
    ..We presume that these pathomechanisms may be involved to a certain extent in the neurological damage found in patients affected by SCADD and EE. ..
  65. Capendeguy O, Iwaszkiewicz J, Michielin O, Horisberger J. The fourth extracellular loop of the alpha subunit of Na,K-ATPase. Functional evidence for close proximity with the second extracellular loop. J Biol Chem. 2008;283:27850-8 pubmed publisher
  66. Silva P, Muzi Filho H, Pereira Acácio A, Dias J, Martins J, Landim Vieira M, et al. Altered signaling pathways linked to angiotensin II underpin the upregulation of renal Na(+)-ATPase in chronically undernourished rats. Biochim Biophys Acta. 2014;1842:2357-66 pubmed publisher
  67. Samani N, Gauguier D, Vincent M, Kaiser M, Bihoreau M, Lodwick D, et al. Analysis of quantitative trait loci for blood pressure on rat chromosomes 2 and 13. Age-related differences in effect. Hypertension. 1996;28:1118-22 pubmed
  68. Yagawa Y, Kawakami K, Nagano K. Cloning and analysis of the 5'-flanking region of rat Na+/K(+)-ATPase alpha 1 subunit gene. Biochim Biophys Acta. 1990;1049:286-92 pubmed
    ..The TATA-like box, Sp1 binding site and the active transcriptional factor (ATF) consensus site in this region were conserved in both rat and horse. ..
  69. Morton M, Farr G, Hull M, Capendeguy O, Horisberger J, Caplan M. Association with {beta}-COP regulates the trafficking of the newly synthesized Na,K-ATPase. J Biol Chem. 2010;285:33737-46 pubmed publisher
    ..Although the Lys(54) ?-subunit reaches the cell surface without need for ?-subunit assembly, it is only functional as an ion-transporting ATPase in the presence of the ?-subunit. ..
  70. Swift F, Tovsrud N, Enger U, Sjaastad I, Sejersted O. The Na+/K+-ATPase alpha2-isoform regulates cardiac contractility in rat cardiomyocytes. Cardiovasc Res. 2007;75:109-17 pubmed
    ..The alpha2-isoform of the NKA is functionally coupled to the NCX and can regulate Ca2+ handling without changing global [Na+]i. ..
  71. Juel C, Nordsborg N, Bangsbo J. Purinergic effects on Na,K-ATPase activity differ in rat and human skeletal muscle. PLoS ONE. 2014;9:e91175 pubmed publisher
    ..Rat muscle is not a reliable model for purinergic effects on Na,K-ATPase in human skeletal muscle...
  72. Redina O, Khvorostova I, Dymshits G, Markel A. [A search for genetic loci responsible for emotional stress-induced arterial hypertension in the ISIAH rats]. Genetika. 2003;39:813-8 pubmed
    ..of cosegregation analysis for the following loci: the gene for the Na+, K(+)-ATPase alpha 1 subunit isoform (Atp1a1), the endothelin-2 gene (Edn2), the low affinity nerve growth factor receptor gene (Lngfr), and a region of ..
  73. Zhang S, Malmersjö S, Li J, Ando H, Aizman O, Uhlen P, et al. Distinct role of the N-terminal tail of the Na,K-ATPase catalytic subunit as a signal transducer. J Biol Chem. 2006;281:21954-62 pubmed
    ..Thus we have identified a well conserved Na,K-ATPase motif that binds to the inositol 1,4,5-trisphosphate receptor and can trigger an anti-apoptotic calcium signal. ..
  74. Deng H, Yang Z, Li Y, Bao G, Friedrich T, Gu Q, et al. Interactions of Na+,K+-ATPase and co-expressed delta-opioid receptor. Neurosci Res. 2009;65:222-7 pubmed publisher
    ..17 to 0.27muM. In conclusion, pump activity not only affects neural activity directly but our results also suggest that pump activity is affected through functional interaction with DOR that will modulate pain sensation. ..
  75. He S, Shelly D, Moseley A, James P, James J, Paul R, et al. The alpha(1)- and alpha(2)-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility. Am J Physiol Regul Integr Comp Physiol. 2001;281:R917-25 pubmed
    ..These results demonstrate that the Na-K-ATPase alpha(1)- and alpha(2)-isoforms may play different roles in skeletal muscle contraction...
  76. Fujii T, Takahashi Y, Itomi Y, Fujita K, Morii M, Tabuchi Y, et al. K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells. J Biol Chem. 2008;283:6869-77 pubmed publisher
    ..In conclusion, KCC3a forms a functional complex with alpha1NaK in the basolateral membrane of luminal parietal cells, and it up-regulates alpha1NaK in lipid rafts, whereas KCC3a is absent in basal parietal cells. ..
  77. Qiu L, Krieger E, Schaftenaar G, Swarts H, Willems P, De Pont J, et al. Reconstruction of the complete ouabain-binding pocket of Na,K-ATPase in gastric H,K-ATPase by substitution of only seven amino acids. J Biol Chem. 2005;280:32349-55 pubmed
    ..Based on the E(2)P crystal structure of Ca(2+)-ATPase we constructed a homology model for the ouabain-binding site of Na,K-ATPase involving all seven amino acids as well as several earlier postulated amino acids. ..
  78. Scherer E, Loureiro S, Vuaden F, Schmitz F, Kolling J, Siebert C, et al. Mild hyperhomocysteinemia reduces the activity and immunocontent, but does not alter the gene expression, of catalytic ? subunits of cerebral Na+,K+-ATPase. Mol Cell Biochem. 2013;378:91-7 pubmed publisher
    ..The present findings support that the homocysteine modulates the Na(+),K(+)-ATPase and this could be associated, at least in part, with the risk to the development of cerebral diseases in individuals with mild hyperhomocysteinemia. ..
  79. Yuan X, Luo S, Lin Z, Wu Y. Cyclic stretch translocates the alpha2-subunit of the Na pump to plasma membrane in skeletal muscle cells in vitro. Biochem Biophys Res Commun. 2006;348:750-7 pubmed
  80. Queiroz Madeira E, Lara L, Wengert M, Landgraf S, Líbano Soares J, Zapata Sudo G, et al. Na(+)-ATPase in spontaneous hypertensive rats: possible AT(1) receptor target in the development of hypertension. Biochim Biophys Acta. 2010;1798:360-6 pubmed publisher
    ..Our results open new possibilities towards our understanding of the pathophysiological mechanisms involved in the increased sodium reabsorption in PT found in essential hypertension. ..
  81. Beguin P, Crambert G, Monnet Tschudi F, Uldry M, Horisberger J, Garty H, et al. FXYD7 is a brain-specific regulator of Na,K-ATPase alpha 1-beta isozymes. EMBO J. 2002;21:3264-73 pubmed
    ..These data suggest that FXYD7 is a novel, tissue- and isoform-specific Na,K-ATPase regulator which could play an important role in neuronal excitability. ..
  82. Zampar G, Chesta M, Carbajal A, Chanaday N, Díaz N, Casale C, et al. Acetylated tubulin associates with the fifth cytoplasmic domain of Na(+)/K(+)-ATPase: possible anchorage site of microtubules to the plasma membrane. Biochem J. 2009;422:129-37 pubmed publisher
    ..Taken together, our results are consistent with the idea that NKA may act as a microtubule-plasma membrane anchorage site through an interaction between acetylated tubulin and CD5. ..
  83. Herrera V, Emanuel J, Ruiz Opazo N, Levenson R, Nadal Ginard B. Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications. J Cell Biol. 1987;105:1855-65 pubmed
    ..Isoform diversity of the alpha subunit may provide a biochemical basis for Na,K-ATPase functional diversity. ..
  84. Lecuona E, Minin A, Trejo H, Chen J, Comellas A, Sun H, et al. Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells. J Cell Sci. 2009;122:3915-22 pubmed publisher
  85. Cai T, Wang H, Chen Y, Liu L, Gunning W, Quintas L, et al. Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase. J Cell Biol. 2008;182:1153-69 pubmed publisher
    ..Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. ..
  86. Azarias G, Kruusmägi M, Connor S, Akkuratov E, Liu X, Lyons D, et al. A specific and essential role for Na,K-ATPase ?3 in neurons co-expressing ?1 and ?3. J Biol Chem. 2013;288:2734-43 pubmed publisher